FastQCFastQC Report
Thu 26 May 2016
SRR1660594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32574266
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14227634.367751525084249No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG8845282.7154195891935062Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA7529032.3113429478349565Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA3032440.9309311835299681Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2689580.8256763176183309Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2423100.7438694090605142Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1567860.4813185967106673Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC1096110.3364956865029591Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA921140.2827815061128315Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT593490.1821959702791154Illumina Single End PCR Primer 1 (100% over 37bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG586980.18019746016686913Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT457200.14035619405821761Illumina Single End PCR Primer 1 (100% over 37bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT355960.10927644540018186Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2512900.087.470758
AGTGGTA2536350.086.8005455
TGGTATC2527200.086.778397
GTGGTAT2535900.086.618146
GTATCAA2567250.085.540169
AAGCAGT2626900.084.722691
GCAGTGG2651500.083.657873
CAGTGGT2663150.082.79314
AGCAGTG2727400.081.5249562
CAACGCA2477300.045.62534712-13
ATCAACG2499200.045.2901710-11
ACGCAGA2512100.044.9881614-15
TCAACGC2473350.044.48712512-13
AGAGTAC2448350.044.46928418-19
AACGCAG2505100.043.88114514-15
CGCAGAG2527100.043.3918216-17
TATCAAC2534600.043.28517510-11
GAGTACT1866800.042.6135320-21
AGTACTT1946950.042.2545920-21
GCAGAGT2663700.041.98716416-17
Produced by FastQC (version 0.11.5)