##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660594_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32574266 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.245238987119464 34.0 31.0 34.0 30.0 34.0 2 32.39627278171057 34.0 31.0 34.0 30.0 34.0 3 32.41115993219924 34.0 31.0 34.0 30.0 34.0 4 35.84950954842697 37.0 35.0 37.0 35.0 37.0 5 35.86012341766964 37.0 35.0 37.0 35.0 37.0 6 35.89073116797167 37.0 35.0 37.0 35.0 37.0 7 35.76699069750336 37.0 35.0 37.0 35.0 37.0 8 35.84547667167696 37.0 35.0 37.0 35.0 37.0 9 37.55910211453421 39.0 38.0 39.0 35.0 39.0 10-11 37.44283158982002 39.0 37.0 39.0 34.5 39.0 12-13 37.536282598048416 39.0 38.0 39.0 35.0 39.0 14-15 38.961725553539715 40.0 39.0 41.0 36.0 41.0 16-17 38.85314341388383 40.0 38.5 41.0 35.0 41.0 18-19 38.66268188514209 40.0 38.0 41.0 34.0 41.0 20-21 38.37121025228934 40.0 38.0 41.0 33.5 41.0 22-23 38.02429204390975 40.0 37.5 41.0 32.5 41.0 24-25 37.89604643739325 40.0 38.0 41.0 32.0 41.0 26-27 37.73433599394073 40.0 38.0 41.0 32.0 41.0 28-29 37.610658088197596 40.0 37.5 41.0 31.5 41.0 30-31 37.33540376627366 40.0 37.0 41.0 30.5 41.0 32-33 37.24110933151955 40.0 37.0 41.0 30.0 41.0 34-35 37.27862435948672 40.0 37.0 41.0 30.0 41.0 36-37 37.03649799814369 40.0 37.0 41.0 30.0 41.0 38-39 36.5745518717137 39.5 35.0 41.0 29.5 41.0 40-41 36.56653514464455 39.0 35.0 41.0 29.5 41.0 42-43 36.41009378384766 39.0 35.0 41.0 29.0 41.0 44-45 36.340940268615725 39.0 35.0 41.0 29.0 41.0 46-47 36.23358171754354 39.0 35.0 41.0 28.0 41.0 48-49 36.14206751427645 39.0 35.0 41.0 27.5 41.0 50-51 35.123297728335615 38.0 34.0 40.0 26.0 40.5 52-53 35.1884126414391 38.5 34.0 39.5 26.0 40.5 54-55 35.608219844462496 39.0 35.0 41.0 25.5 41.0 56-57 35.323792729512306 39.0 34.5 41.0 24.0 41.0 58-59 34.94249503580526 38.5 34.0 41.0 22.5 41.0 60-61 34.564930242787355 38.0 33.5 40.5 21.5 41.0 62-63 34.14235763593261 37.0 33.0 40.0 20.5 41.0 64-65 33.701359610067655 36.5 32.0 40.0 20.0 41.0 66-67 33.464298949974804 36.0 32.0 39.5 20.0 41.0 68-69 33.12811100639996 35.5 32.0 39.0 20.0 41.0 70-71 32.84190868644592 35.0 31.5 39.0 20.0 41.0 72-73 32.5800652269494 35.0 31.5 38.0 20.0 40.0 74-75 32.22380954032855 35.0 31.0 37.0 19.0 39.5 76-77 31.891672570611412 35.0 31.0 37.0 18.0 39.0 78-79 31.47595862942852 35.0 31.0 36.0 16.5 39.0 80-81 30.97304699052927 35.0 30.5 36.0 13.5 37.5 82-83 30.273177575206148 35.0 29.0 35.0 10.0 37.0 84-85 29.565535736093025 34.0 28.0 35.0 9.0 37.0 86-87 29.017216934373902 34.0 26.5 35.0 8.0 36.0 88-89 28.62966866237293 34.0 25.5 35.0 7.0 36.0 90-91 28.305151081531662 34.0 25.0 35.0 7.0 36.0 92-93 28.05348458197032 34.0 24.5 35.0 7.0 35.0 94-95 27.83134738937786 34.0 24.0 35.0 7.0 35.0 96-97 27.617912725953673 34.0 23.5 35.0 7.0 35.0 98-99 27.360699148217186 34.0 23.0 35.0 7.0 35.0 100 27.256186094876245 34.0 20.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2613862732236356 1101 2 0.2399976364474199 1101 3 0.23776978537725313 1101 4 0.2294826343641958 1101 5 0.23043308724484035 1101 6 0.17279933821551907 1101 7 0.19453481706603526 1101 8 0.19625324969533864 1101 9 0.2710678386506373 1101 10-11 0.2803115230024815 1101 12-13 0.2816300739323623 1101 14-15 0.3203832706791445 1101 16-17 0.3441084773139025 1101 18-19 0.3501566429965308 1101 20-21 0.3626281231240327 1101 22-23 0.3681830154582926 1101 24-25 0.2557055186121957 1101 26-27 0.3006009836336503 1101 28-29 0.36487462379455593 1101 30-31 0.3623273464726182 1101 32-33 0.34087911749066535 1101 34-35 0.3266299453247896 1101 36-37 0.2901233187888508 1101 38-39 0.23960531218475722 1101 40-41 0.2919612878714233 1101 42-43 0.33538353978722313 1101 44-45 0.3213091920019977 1101 46-47 0.3307325757151034 1101 48-49 0.295555384634163 1101 50-51 0.2683426250700265 1101 52-53 0.3268949806845569 1101 54-55 0.41057304017754603 1101 56-57 0.4108023190624479 1101 58-59 0.378200126148279 1101 60-61 0.3188479851513435 1101 62-63 0.32432113984815203 1101 64-65 0.28433197397599486 1101 66-67 0.2891085539891378 1101 68-69 0.2913338089617312 1101 70-71 0.19480978749274414 1101 72-73 0.17704938433031003 1101 74-75 0.10121904561464845 1101 76-77 0.08835697058571768 1101 78-79 0.11536578757700866 1101 80-81 0.05596029194931873 1101 82-83 -0.012162807261020703 1101 84-85 -0.10699925959605494 1101 86-87 -0.05116700667626617 1101 88-89 0.0018730923438958769 1101 90-91 -0.010601762434248485 1101 92-93 -0.03853053206489676 1101 94-95 -0.11901622211619767 1101 96-97 -0.1897447677812636 1101 98-99 -0.031024187289087735 1101 100 -0.08222479872472732 1102 1 0.22812296226715745 1102 2 0.20587047735808284 1102 3 0.18647720235359344 1102 4 0.16412732120711837 1102 5 0.1507186471711961 1102 6 0.18508089157356977 1102 7 0.18860211349027622 1102 8 0.1715904733157103 1102 9 0.21153341771331213 1102 10-11 0.20064123684339563 1102 12-13 0.21545380800558434 1102 14-15 0.21983389872166015 1102 16-17 0.23571027152000568 1102 18-19 0.25181041759510236 1102 20-21 0.25994802996459754 1102 22-23 0.2624170398282857 1102 24-25 0.2137051123251794 1102 26-27 0.26871805630519674 1102 28-29 0.306803841087806 1102 30-31 0.28431593886116246 1102 32-33 0.2780147375326578 1102 34-35 0.25889511088516315 1102 36-37 0.20040458695978458 1102 38-39 0.22266540354480213 1102 40-41 0.24398915023420642 1102 42-43 0.2668533040247212 1102 44-45 0.24241089557337858 1102 46-47 0.1664447836194256 1102 48-49 0.1854377376573879 1102 50-51 0.1939125824270107 1102 52-53 0.25934592999055184 1102 54-55 0.29970876470784447 1102 56-57 0.2988791326714946 1102 58-59 0.2733747607768535 1102 60-61 0.27496700188854817 1102 62-63 0.2339508144235225 1102 64-65 0.1740111359341867 1102 66-67 0.24329565309533763 1102 68-69 0.2067216130342544 1102 70-71 0.17916700787829143 1102 72-73 0.17179804210265814 1102 74-75 0.07925184860011569 1102 76-77 0.06968755235157786 1102 78-79 0.11307753504806684 1102 80-81 0.03392365586640267 1102 82-83 -0.00402031565511507 1102 84-85 -0.06611217993558327 1102 86-87 -0.07929323412053435 1102 88-89 -0.001367981879507596 1102 90-91 -0.02946143116195543 1102 92-93 -0.03399279799890209 1102 94-95 -0.04182915299965728 1102 96-97 -0.06323673628945414 1102 98-99 0.014705316345210662 1102 100 -0.038298919731182934 1103 1 0.17700649241138677 1103 2 0.1494116740889524 1103 3 0.16969428687387023 1103 4 0.18651702314685537 1103 5 0.171528884425463 1103 6 0.12657596715427388 1103 7 0.1844238890814438 1103 8 0.17271705896532552 1103 9 0.21441692685707636 1103 10-11 0.19262801236971683 1103 12-13 0.2240781294739378 1103 14-15 0.25069608559823564 1103 16-17 0.2528269556342977 1103 18-19 0.2615592008303125 1103 20-21 0.29523618621518466 1103 22-23 0.22298517486132852 1103 24-25 0.24454021373743018 1103 26-27 0.23750175980349297 1103 28-29 0.2809210096429453 1103 30-31 0.24441559547424418 1103 32-33 0.2646337767854021 1103 34-35 0.26019485073733506 1103 36-37 0.23060093750699906 1103 38-39 0.28086587196761315 1103 40-41 0.2366454750647904 1103 42-43 0.28576387574988615 1103 44-45 0.25766321339567355 1103 46-47 0.2717520488252205 1103 48-49 0.26468013296832993 1103 50-51 0.19959855518187197 1103 52-53 0.2847199365772397 1103 54-55 0.35641509291401974 1103 56-57 0.3932623728247435 1103 58-59 0.30987785803355905 1103 60-61 0.29293979044589946 1103 62-63 0.2343896727771977 1103 64-65 0.21012312522243803 1103 66-67 0.22444421637818124 1103 68-69 0.21586106255586657 1103 70-71 0.1923795205804808 1103 72-73 0.12232972605125525 1103 74-75 0.13978708331397627 1103 76-77 0.16101234937531572 1103 78-79 0.22493131713074987 1103 80-81 0.16608663322952566 1103 82-83 0.04557958944919349 1103 84-85 0.03952647332252113 1103 86-87 0.13130831585304392 1103 88-89 0.12411785890003912 1103 90-91 0.14319547356501516 1103 92-93 0.07918079050253368 1103 94-95 0.07946333888102686 1103 96-97 0.06446156691585259 1103 98-99 0.20056986809648336 1103 100 0.1283889393127282 1104 1 0.20907710996767292 1104 2 0.18298801948779442 1104 3 0.16024768041893367 1104 4 0.158547818349156 1104 5 0.155553632632774 1104 6 0.166044592160425 1104 7 0.15480131189533353 1104 8 0.12536973011325614 1104 9 0.20155070015240995 1104 10-11 0.19317393503322933 1104 12-13 0.20482954967331324 1104 14-15 0.22959938485786324 1104 16-17 0.2406491060434206 1104 18-19 0.19568888280284824 1104 20-21 0.21605484965596844 1104 22-23 0.23413439572656358 1104 24-25 0.2058097461290842 1104 26-27 0.22069455656089332 1104 28-29 0.22897922571878127 1104 30-31 0.24142320617790602 1104 32-33 0.18059520917532268 1104 34-35 0.2332206457135655 1104 36-37 0.21382615487259216 1104 38-39 0.18766574498214794 1104 40-41 0.24111722950141967 1104 42-43 0.2469463542180037 1104 44-45 0.18647016529818927 1104 46-47 0.20048551898798905 1104 48-49 0.19060800177273762 1104 50-51 0.14352779150043915 1104 52-53 0.21596317091589157 1104 54-55 0.26036455005771586 1104 56-57 0.25613672381837915 1104 58-59 0.27191021653172953 1104 60-61 0.22915669332000022 1104 62-63 0.2134095864367822 1104 64-65 0.20169559521963976 1104 66-67 0.1766983018301147 1104 68-69 0.19329359383699085 1104 70-71 0.20090245942172658 1104 72-73 0.14607670570846665 1104 74-75 0.11949069912770227 1104 76-77 0.08325088293738858 1104 78-79 0.16504909861709294 1104 80-81 0.10175352472941412 1104 82-83 0.024219312563829476 1104 84-85 -0.0392343145964098 1104 86-87 0.03538765893997109 1104 88-89 -0.03050595828356606 1104 90-91 0.02305388072726089 1104 92-93 0.04451301662626861 1104 94-95 0.030757452645335803 1104 96-97 0.0632680738820035 1104 98-99 0.11967747201989098 1104 100 0.05973525460393603 1105 1 0.1319784961974193 1105 2 0.1404454119158629 1105 3 0.1456541522024466 1105 4 0.12477733300600136 1105 5 0.12818855805364393 1105 6 0.1265356971985696 1105 7 0.12790283553727022 1105 8 0.1513254215456712 1105 9 0.16699126565723788 1105 10-11 0.15169573357584198 1105 12-13 0.15649502209873134 1105 14-15 0.20458679036340754 1105 16-17 0.20064965481219588 1105 18-19 0.21492408259143048 1105 20-21 0.1686244531244796 1105 22-23 0.21295395441939746 1105 24-25 0.20554425052943515 1105 26-27 0.2022569408236592 1105 28-29 0.2078283345538523 1105 30-31 0.18391859702141033 1105 32-33 0.20546581449329437 1105 34-35 0.18931165347054701 1105 36-37 0.18303207661120524 1105 38-39 0.2072951402355656 1105 40-41 0.2178187834352059 1105 42-43 0.285106918450829 1105 44-45 0.18627509948419885 1105 46-47 0.19395136065216434 1105 48-49 0.20695206102593744 1105 50-51 0.14287437248641055 1105 52-53 0.2133642263369353 1105 54-55 0.26056179447429884 1105 56-57 0.25146982585975053 1105 58-59 0.2258626864881208 1105 60-61 0.2519809486830056 1105 62-63 0.23465609813102617 1105 64-65 0.2421463511339752 1105 66-67 0.2135324452873988 1105 68-69 0.26919485628614126 1105 70-71 0.21887453799905643 1105 72-73 0.12398976338074164 1105 74-75 0.10420343979811975 1105 76-77 0.10418444409943461 1105 78-79 0.11448942761988334 1105 80-81 0.07340261904236911 1105 82-83 -0.011629770768479375 1105 84-85 0.027080549736989923 1105 86-87 0.0874989166660356 1105 88-89 0.03718664325287335 1105 90-91 0.07137915650648807 1105 92-93 0.13453333034894754 1105 94-95 0.13409178751804873 1105 96-97 0.12426550447769813 1105 98-99 0.20316208160238602 1105 100 0.08525574864559715 1106 1 0.0911202371203288 1106 2 0.0883320933699423 1106 3 0.10242019781163236 1106 4 0.10835533477029458 1106 5 0.09999313973345636 1106 6 0.12220653459656461 1106 7 0.09981053834867737 1106 8 0.10149982736360386 1106 9 0.1357821040335878 1106 10-11 0.09962031603171795 1106 12-13 0.14032160092060053 1106 14-15 0.14824471657821192 1106 16-17 0.14806490117217663 1106 18-19 0.13082857923271973 1106 20-21 0.13593084845799552 1106 22-23 0.12967132520274305 1106 24-25 0.08564618684153658 1106 26-27 0.1177360608419562 1106 28-29 0.0747237352487744 1106 30-31 0.07001723521747039 1106 32-33 0.07295443828554937 1106 34-35 0.10074090384558332 1106 36-37 0.07816696764989217 1106 38-39 0.07880701996656825 1106 40-41 0.11151187435942944 1106 42-43 0.09966290689576596 1106 44-45 0.10209106260951017 1106 46-47 0.11444452293583396 1106 48-49 0.12821191541413413 1106 50-51 0.11886692414446287 1106 52-53 0.11597560640307591 1106 54-55 0.1521808730705061 1106 56-57 0.11327591826158567 1106 58-59 0.11003321666662913 1106 60-61 0.12539986496778965 1106 62-63 0.08953575716878959 1106 64-65 0.06308143417673051 1106 66-67 0.06491270484023914 1106 68-69 0.10754989230779444 1106 70-71 0.10779268090506378 1106 72-73 0.0067171686250659945 1106 74-75 -0.03694632570962142 1106 76-77 -0.04594693912316572 1106 78-79 0.01600470940036658 1106 80-81 -0.013727072388043382 1106 82-83 -0.0028165021356798547 1106 84-85 -0.1005173276475766 1106 86-87 -0.071308481571144 1106 88-89 -0.08103298541015747 1106 90-91 -0.07388397666508695 1106 92-93 -0.03221889346446716 1106 94-95 -0.061722578691142616 1106 96-97 -0.07549725101754134 1106 98-99 -0.008627264890350261 1106 100 -0.03369114035639953 1107 1 0.04993558772562068 1107 2 0.060952085962746594 1107 3 0.06185113363658701 1107 4 0.06714553799061918 1107 5 0.06562827847510988 1107 6 0.09365861579783541 1107 7 0.10029631903227454 1107 8 0.06466165135598345 1107 9 0.1064037603109469 1107 10-11 0.10865908373394717 1107 12-13 0.09324679748915088 1107 14-15 0.08507121043402321 1107 16-17 0.050270948910345226 1107 18-19 0.08044888607118139 1107 20-21 0.03450518761463428 1107 22-23 0.06756754853362423 1107 24-25 0.06133892041331279 1107 26-27 0.0031429521687655892 1107 28-29 -0.008353764342153625 1107 30-31 -0.0026600043674989138 1107 32-33 7.572867997538424E-4 1107 34-35 -0.00464462139655808 1107 36-37 -0.015307109989528556 1107 38-39 -0.016592906374945926 1107 40-41 -0.04585221756213542 1107 42-43 -0.016065083554344994 1107 44-45 -0.045220070710477955 1107 46-47 0.0037898280288430897 1107 48-49 -0.007251774027558611 1107 50-51 -0.09296041366177832 1107 52-53 -0.005076085248070683 1107 54-55 0.0890232033874625 1107 56-57 0.06142348994197988 1107 58-59 -0.00952871484167872 1107 60-61 0.005205642824883228 1107 62-63 0.028785546189070033 1107 64-65 0.02279680688896235 1107 66-67 0.026090605215749463 1107 68-69 0.03991061903068527 1107 70-71 0.039689698962760644 1107 72-73 0.003725003631366519 1107 74-75 -0.035235070623265585 1107 76-77 -0.03841132883904663 1107 78-79 0.012543827667755636 1107 80-81 -0.08047225631318256 1107 82-83 -0.0690606113957628 1107 84-85 -0.17478102634969517 1107 86-87 -0.1649411289778584 1107 88-89 -0.1573487703000147 1107 90-91 -0.14141611020684763 1107 92-93 -0.15797376157299325 1107 94-95 -0.21779868430461136 1107 96-97 -0.1996758324891026 1107 98-99 -0.109145449956074 1107 100 -0.1380537003370712 1108 1 0.010055579172636442 1108 2 0.03606161067141045 1108 3 -0.008273324597112719 1108 4 0.030130266262993644 1108 5 0.006332984128199826 1108 6 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84-85 -0.3101583766228231 2316 86-87 -0.3315861228614416 2316 88-89 -0.36491101336089926 2316 90-91 -0.3949549447314453 2316 92-93 -0.39555156812830106 2316 94-95 -0.4048897311836903 2316 96-97 -0.4775006062682259 2316 98-99 -0.40369001744891264 2316 100 -0.49912256522609155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1122.0 3 1069.0 4 959.0 5 1267.0 6 2088.0 7 1630.0 8 3324.0 9 10970.0 10 19166.0 11 19117.0 12 21981.0 13 27940.0 14 36513.0 15 49844.0 16 71441.0 17 105181.0 18 148253.0 19 198259.0 20 253974.0 21 310609.0 22 378537.0 23 482361.0 24 640771.0 25 780873.0 26 812231.0 27 771081.0 28 765837.0 29 854075.0 30 1045124.0 31 1107378.0 32 901298.0 33 878589.0 34 1189366.0 35 1848112.0 36 3284881.0 37 6136623.0 38 7293423.0 39 2114430.0 40 4569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.39372900289173 50.04575491556685 14.345385301351616 18.2151307801898 2 18.97373498725299 32.05145624709198 34.528703245079924 14.44610552057511 3 25.000122816597237 36.66328122716379 20.211669492676567 18.124926463562403 4 17.53041855424655 23.499769296360654 34.02115706502723 24.948655084365566 5 18.47546550067066 43.34248811640507 21.361343690537506 16.820702692386764 6 25.14078505340434 22.208239253014085 34.969746004476306 17.681229689105262 7 18.390307852050558 35.566674781343536 28.333608581453472 17.70940878515243 8 24.39273922762244 21.870662606712788 35.80581949714245 17.930778668522322 9 24.366423392398147 34.325995706563496 23.354351268190886 17.953229632847474 10-11 17.68727595095972 32.476430253180986 32.333292762334985 17.50300103352431 12-13 18.007077568138293 28.92601487156018 31.77220108555951 21.29470647474202 14-15 18.295877080076966 35.755522814440106 27.932655417968544 18.015944687514384 16-17 22.667580461171998 27.778275832796172 27.604522346389725 21.949621359642098 18-19 20.439714178045378 31.002304501619932 26.326901851066875 22.23107946926782 20-21 24.958492018638655 30.60047818840933 25.214455727947843 19.226574065004172 22-23 26.026702331124056 26.1428194548583 28.27096737414136 19.55951083987629 24-25 23.371351065181646 30.59106630266738 23.4014374414295 22.636145190721475 26-27 22.414556673621984 28.688504011150005 29.616152365579236 19.280786949648775 28-29 25.510589256961236 25.63302998058008 28.678582520909547 20.177798241549137 30-31 25.663454186436763 24.121089009991685 29.613437491530664 20.602019312040884 32-33 25.05905361057847 24.3202118256363 30.21817261045468 20.402561953330554 34-35 25.340951429606985 24.27872372229921 29.798241386674075 20.58208346141973 36-37 24.63766965259201 25.260026319913308 30.608384925077075 19.49391910241761 38-39 24.97476735335481 26.24217364121088 29.637511374769304 19.145547630665 40-41 26.518527647818342 26.33821231734368 28.288563126443172 18.854696908394804 42-43 24.78096814275975 28.033648479760497 28.45332316304972 18.73206021443003 44-45 24.349952224769307 28.287875894644237 28.802266143075595 18.55990573751086 46-47 25.122310326960413 26.787656359392138 29.475006501808387 18.615026811839066 48-49 24.767399042479255 25.51473144517121 30.701681500593793 19.01618801175574 50-51 24.3768833397972 25.949130762939337 30.366753443923923 19.307232453339545 52-53 23.771921839230096 26.753987890690077 29.78898411859714 19.685106151482685 54-55 23.212648623572214 25.803074388787728 30.19227430784849 20.79200267979157 56-57 24.25265190189811 24.806384090049107 28.907229195002166 22.033734813050614 58-59 25.52404559211817 24.97885680301531 27.759008482388474 21.73808912247804 60-61 26.324638442971576 24.70959497630081 27.4950813187817 21.47068526194591 62-63 25.612695873823498 24.65508053225929 27.248152277606387 22.484071316310825 64-65 24.11241569022869 24.79780320458905 27.565778936213047 23.52400216896921 66-67 24.51298221126021 24.92729967223 26.934541923202985 23.625176193306807 68-69 23.242846033068208 25.899390506097397 28.30409181599943 22.553671644834967 70-71 21.62875140360656 26.831368584913477 29.68234494279825 21.85753506868171 72-73 21.049533221830252 27.674813376505412 29.485354888390663 21.79029851327367 74-75 20.678196905503597 29.895345521222865 29.184555889543866 20.241901683729665 76-77 20.394311191465263 31.974995976096647 27.773934882229668 19.856757950208422 78-79 20.314648222656736 34.093558137546424 26.000817806274835 19.590975833522005 80-81 20.309092834516708 35.00382624273408 25.36061483439492 19.326466088354298 82-83 20.447857687586392 35.448144670697516 24.83111040070568 19.272887241010412 84-85 20.68259694831491 35.306265657909144 24.545379720648857 19.465757673127097 86-87 21.090881562918767 34.8710412506181 24.464495697688985 19.573581488774145 88-89 21.633771814802635 34.16081716082833 24.43512696301412 19.770284061354918 90-91 22.190989486936587 33.328854545577144 24.371742885696776 20.1084130817895 92-93 22.625400647831427 32.69989600919265 24.441115838903055 20.23358750407287 94-95 23.08897036758314 32.22249301456122 24.355723992281796 20.332812625573844 96-97 23.458134007229283 31.37931800351935 24.530323259566163 20.632224729685202 98-99 23.985336703963053 30.736821655751196 24.544536757341326 20.733304882944424 100 24.244898062002537 30.573132414164416 24.45909821814138 20.722871305691665 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4174.0 1 4682.5 2 4696.5 3 4160.5 4 4332.5 5 4723.0 6 5122.5 7 5391.0 8 5605.0 9 5850.0 10 6183.5 11 8768.0 12 83298.0 13 105316.5 14 56085.5 15 61631.5 16 71712.5 17 81511.0 18 90255.5 19 98805.5 20 108512.5 21 122397.5 22 140376.5 23 161747.0 24 184979.5 25 209762.5 26 238968.0 27 276185.0 28 324590.0 29 380216.5 30 470390.5 31 619866.5 32 811813.5 33 1007267.0 34 1154926.5 35 1226407.0 36 1223579.0 37 1159244.0 38 1081616.5 39 1012159.5 40 951871.0 41 912818.5 42 898729.0 43 892692.0 44 892599.5 45 913481.0 46 932063.0 47 945987.5 48 950498.0 49 949075.0 50 952815.5 51 956821.5 52 957740.0 53 936402.0 54 899143.5 55 865470.0 56 827577.5 57 772476.0 58 697421.5 59 608149.5 60 504937.5 61 407574.0 62 325939.0 63 252492.5 64 190706.0 65 141427.5 66 103779.5 67 76956.0 68 57356.0 69 42934.5 70 32419.0 71 24576.5 72 18919.5 73 14371.5 74 11184.0 75 9260.0 76 7127.0 77 4509.0 78 2685.5 79 1799.0 80 1204.0 81 824.5 82 571.0 83 409.0 84 311.5 85 223.0 86 161.5 87 127.0 88 97.0 89 70.5 90 64.5 91 58.0 92 39.5 93 25.5 94 20.0 95 15.5 96 12.0 97 8.0 98 6.0 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04245682773020887 2 0.02156610374582193 3 0.01650996525907905 4 0.01332954056432154 5 0.010173675133616212 6 0.01133410036008179 7 0.011214373947827405 8 0.006566533225952045 9 0.003963251236420799 10-11 0.0053339651613331825 12-13 0.008495970408051558 14-15 0.0037529625379739942 16-17 0.009581182888357331 18-19 0.03523333419086097 20-21 0.05672883005253288 22-23 0.053671201678036275 24-25 0.04506164467374338 26-27 0.03942836348177423 28-29 0.03552037058947084 30-31 0.03665009673587119 32-33 0.04499410669759988 34-35 0.045628042700946815 36-37 0.050371050571024374 38-39 0.043792237713046245 40-41 0.04068242090243875 42-43 0.03909681341706978 44-45 0.058992887207343365 46-47 0.055071079729010625 48-49 0.058144057643539845 50-51 0.06210270401795086 52-53 0.06157467984082895 54-55 0.05068418118768969 56-57 0.05166808670378022 58-59 0.05250617158956091 60-61 0.05066729669365382 62-63 0.05038486515705373 64-65 0.0461130881659774 66-67 0.05214238749078798 68-69 0.04944393835305453 70-71 0.05649091218202738 72-73 0.057752644372708196 74-75 0.07385738177492626 76-77 0.07682444786323045 78-79 0.06170975579311595 80-81 0.05981255264508493 82-83 0.061660637265011586 84-85 0.05965598733675227 86-87 0.05824229469974857 88-89 0.059711245680869676 90-91 0.06389860020176663 92-93 0.06270901084923909 94-95 0.07275067993857483 96-97 0.06650648705330767 98-99 0.06659858429350335 100 0.06897776299855843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.2574266E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.65051280731575 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3902856012399 26.53141089553488 2 12.989176673877678 9.521199720848815 3 4.942292944498659 5.434127125795631 4 2.645774422123568 3.8787595737323293 5 1.6247250017524968 2.977350224254799 6 1.0690087366380772 2.3507831035971765 7 0.7282982856278074 1.868475395146364 8 0.5569627044825671 1.6330374987068437 9 0.41679867168267803 1.3748296549120365 >10 2.3228439400967327 15.357624673261508 >50 0.20468293591418707 5.207863552638272 >100 0.10423213638941478 6.425887708172421 >500 0.0026394259070300023 0.6471793743607268 >1k 0.0017380612612679896 1.3227185823589163 >5k 2.448952614748681E-4 0.6179285765697372 >10k+ 2.9556324655633767E-4 14.8508243401095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1422763 4.367751525084249 No Hit ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG 884528 2.7154195891935062 Illumina Single End PCR Primer 1 (100% over 32bp) ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA 752903 2.3113429478349565 Illumina Single End PCR Primer 1 (100% over 34bp) ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA 303244 0.9309311835299681 Illumina Single End PCR Primer 1 (100% over 36bp) ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG 268958 0.8256763176183309 Illumina Single End PCR Primer 1 (100% over 30bp) ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC 242310 0.7438694090605142 Illumina Single End PCR Primer 1 (100% over 38bp) ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG 156786 0.4813185967106673 Illumina Single End PCR Primer 1 (100% over 28bp) ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC 109611 0.3364956865029591 Illumina Single End PCR Primer 1 (100% over 40bp) ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA 92114 0.2827815061128315 Illumina Single End PCR Primer 1 (100% over 26bp) ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 59349 0.1821959702791154 Illumina Single End PCR Primer 1 (100% over 37bp) ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG 58698 0.18019746016686913 Illumina Single End PCR Primer 1 (100% over 24bp) TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 45720 0.14035619405821761 Illumina Single End PCR Primer 1 (100% over 37bp) ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT 35596 0.10927644540018186 Illumina Single End PCR Primer 1 (100% over 39bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.2848015669792837E-4 1.2279632026090779E-5 0.0 0.0 9.209724019568086E-6 2 4.5127647695883616E-4 1.5349540032613474E-5 0.0 0.0 1.5349540032613474E-5 3 5.832825212393121E-4 2.7629172058704255E-5 0.0 3.0699080065226948E-6 1.5349540032613474E-5 4 6.416107733632433E-4 3.069908006522695E-5 0.0 3.0699080065226948E-6 1.5349540032613474E-5 5 9.670210220546489E-4 4.297871209131773E-5 0.0 3.0699080065226948E-6 1.5349540032613474E-5 6 0.0010499085382307616 5.218843611088582E-5 0.0 6.1398160130453896E-6 1.5349540032613474E-5 7 0.0013262002588178042 5.8328252123931204E-5 0.0 6.1398160130453896E-6 1.5349540032613474E-5 8 0.001498115107183075 6.13981601304539E-5 0.0 6.1398160130453896E-6 1.841944803913617E-5 9 0.04596573258166431 6.753797614349928E-5 0.0 6.1398160130453896E-6 1.841944803913617E-5 10-11 0.2522098272298753 7.674770016306738E-5 0.0 6.1398160130453896E-6 1.841944803913617E-5 12-13 1.045417262817219 7.981760816959007E-5 0.0 1.0744678022829432E-5 2.9164126061965602E-5 14-15 2.3087365959374186 9.363219419894219E-5 0.0 1.5349540032613474E-5 3.530394207501099E-5 16-17 4.309975856401492 1.0284191821851029E-4 0.0 1.841944803913617E-5 3.9908804084795036E-5 18-19 7.314884700702082 1.197264122543851E-4 0.0 1.841944803913617E-5 4.144375808805638E-5 20-21 9.47173759801679 1.2893613627395318E-4 0.0 2.1489356045658865E-5 4.297871209131773E-5 22-23 10.373085613041901 1.396808142967826E-4 0.0 2.1489356045658865E-5 4.7583574101101776E-5 24-25 10.976924238292892 1.4889053831635072E-4 0.0 2.1489356045658865E-5 5.218843611088582E-5 26-27 11.365012798753469 1.5656530833265745E-4 0.0 2.1489356045658865E-5 5.525834411740851E-5 28-29 11.587238834483639 1.6270512434570284E-4 0.0 2.1489356045658865E-5 5.679329812066986E-5 30-31 11.704306706404374 1.7191484836527092E-4 0.0 2.302431004892021E-5 5.986320612719255E-5 32-33 11.769543786496985 1.8265952638810035E-4 0.0 2.609421805544291E-5 6.446806813697659E-5 34-35 11.807909961808502 2.0568383643702057E-4 0.0 2.7629172058704255E-5 6.907293014676063E-5 36-37 11.83115377027989 2.1796346846311135E-4 0.0 2.7629172058704255E-5 7.828265416632873E-5 38-39 11.851433582570976 2.2870814648594077E-4 0.0 2.7629172058704255E-5 8.595742418263546E-5 40-41 11.873923728626764 2.3331300849572482E-4 0.0 3.376898807174964E-5 8.595742418263546E-5 42-43 11.901571320133506 2.4098777851203155E-4 0.0 3.376898807174964E-5 8.595742418263546E-5 44-45 11.934325703609101 2.5787227254790636E-4 1.5349540032613474E-6 3.837385008153369E-5 9.05622861924195E-5 46-47 11.9722605568457 2.762917205870425E-4 3.0699080065226948E-6 4.144375808805638E-5 9.823705620872623E-5 48-49 12.01686785513448 2.8703639860987195E-4 3.0699080065226948E-6 4.4513666094579076E-5 1.0898173423155567E-4 50-51 12.081225406583222 3.008509846392241E-4 3.0699080065226948E-6 4.604862009784042E-5 1.1051668823481702E-4 52-53 12.154540949594995 3.0852575465553086E-4 3.0699080065226948E-6 5.218843611088582E-5 1.1819145825112375E-4 54-55 12.227884121778828 3.131306166653149E-4 3.0699080065226948E-6 5.218843611088582E-5 1.2433127426416914E-4 56-57 12.314691910479272 3.361549267142351E-4 3.0699080065226948E-6 5.218843611088582E-5 1.3507595228699857E-4 58-59 12.415633555641746 3.4689960473706453E-4 3.0699080065226948E-6 5.525834411740851E-5 1.396808142967826E-4 60-61 12.53311433018936 3.561093287566326E-4 3.0699080065226948E-6 5.525834411740851E-5 1.45820630309828E-4 62-63 12.669037577086158 3.6071419076641665E-4 3.0699080065226948E-6 5.8328252123931204E-5 1.6730998635548688E-4 64-65 12.826961319711701 3.714588687892461E-4 3.0699080065226948E-6 5.8328252123931204E-5 1.749847563717936E-4 66-67 13.007823414961983 3.8066859280881416E-4 3.0699080065226948E-6 5.986320612719255E-5 1.8572943439462304E-4 68-69 13.211762008697296 3.883433628251209E-4 3.0699080065226948E-6 6.600302214023794E-5 1.9647411241745247E-4 70-71 13.443078042034777 4.067628108642571E-4 3.0699080065226948E-6 6.753797614349928E-5 2.1182365245006596E-4 72-73 13.704400891182015 4.2825216690991596E-4 3.0699080065226948E-6 7.521274615980603E-5 2.2410328447615673E-4 74-75 13.996134249041866 4.420667529392681E-4 3.0699080065226948E-6 8.135256217285142E-5 2.2717319248267943E-4 76-77 14.320357978288751 4.466716149490521E-4 3.0699080065226948E-6 9.209724019568085E-5 2.363829165022475E-4 78-79 14.678582473661876 4.558813389686202E-4 3.0699080065226948E-6 9.977201021198758E-5 2.4405768651855424E-4 80-81 15.072407157232645 4.6355610898492694E-4 3.0699080065226948E-6 1.0591182622503298E-4 2.5480236454138367E-4 82-83 15.502492059222455 4.789056490175404E-4 3.0699080065226948E-6 1.1051668823481702E-4 2.624771345576904E-4 84-85 15.967216268203863 4.8658041903384714E-4 3.0699080065226948E-6 1.1665650424786241E-4 2.716868585772585E-4 86-87 16.47004878022424 4.942551890501539E-4 3.0699080065226948E-6 1.2126136625764645E-4 2.885713526131333E-4 88 16.86952209452701 5.065348210762446E-4 3.0699080065226948E-6 1.227963202609078E-4 2.9164126061965605E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 251290 0.0 87.47075 8 AGTGGTA 253635 0.0 86.800545 5 TGGTATC 252720 0.0 86.77839 7 GTGGTAT 253590 0.0 86.61814 6 GTATCAA 256725 0.0 85.54016 9 AAGCAGT 262690 0.0 84.72269 1 GCAGTGG 265150 0.0 83.65787 3 CAGTGGT 266315 0.0 82.7931 4 AGCAGTG 272740 0.0 81.524956 2 CAACGCA 247730 0.0 45.625347 12-13 ATCAACG 249920 0.0 45.29017 10-11 ACGCAGA 251210 0.0 44.98816 14-15 TCAACGC 247335 0.0 44.487125 12-13 AGAGTAC 244835 0.0 44.469284 18-19 AACGCAG 250510 0.0 43.881145 14-15 CGCAGAG 252710 0.0 43.39182 16-17 TATCAAC 253460 0.0 43.285175 10-11 GAGTACT 186680 0.0 42.61353 20-21 AGTACTT 194695 0.0 42.25459 20-21 GCAGAGT 266370 0.0 41.987164 16-17 >>END_MODULE