FastQCFastQC Report
Thu 26 May 2016
SRR1660594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32574266
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11667883.5819318231146022No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9331372.864644747482568Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7816962.3997348090667647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT3103610.9527797188123901TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG3072040.943088019235798Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT2463190.7561766702586636TruSeq Adapter, Index 21 (100% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC2045890.6280694091464717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1398560.429345054160238Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG1113300.3417728583661716TruSeq Adapter, Index 21 (100% over 39bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA920730.2826556398845641Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT606490.1861868506875949TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT545480.16745734193979997TruSeq Adapter, Index 21 (97% over 40bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG467570.14353968866098163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT466890.14333093491653812TruSeq Adapter, Index 9 (97% over 36bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC362290.11121969716831072TruSeq Adapter, Index 21 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1838650.085.7366648
TGGTATC1861800.084.5999157
GTATCAA1867050.084.379659
AGTGGTA1872100.084.31525
GTGGTAT1874550.084.13236
AAGCAGT1965550.081.4814151
GCAGTGG1985500.080.3637543
CAGTGGT2008600.079.151764
AGCAGTG2073350.077.225372
CAACGCA1816100.045.21840712-13
ATCAACG1827600.044.8104510-11
ACGCAGA1847050.044.34126714-15
TCAACGC1813050.043.71597312-13
AGAGTAC1819350.043.6703818-19
AACGCAG1834700.042.98637414-15
CGCAGAG1863400.042.22467816-17
TATCAAC1870950.042.13955710-11
GAGTACT1472150.041.60832220-21
AGTACTT1560000.041.1557620-21
GCAGAGT2008950.040.36548616-17
Produced by FastQC (version 0.11.5)