##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660594_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32574266 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55709068010926 34.0 31.0 34.0 31.0 34.0 2 32.77235569943464 34.0 31.0 34.0 31.0 34.0 3 32.80520644732256 34.0 31.0 34.0 31.0 34.0 4 36.24398717073164 37.0 37.0 37.0 35.0 37.0 5 36.14293562286254 37.0 35.0 37.0 35.0 37.0 6 36.17372253299583 37.0 36.0 37.0 35.0 37.0 7 36.053095194838775 37.0 35.0 37.0 35.0 37.0 8 36.123598702116574 37.0 35.0 37.0 35.0 37.0 9 37.86739210025485 39.0 38.0 39.0 35.0 39.0 10-11 37.72812699755077 39.0 37.0 39.0 35.0 39.0 12-13 37.82144873195301 39.0 38.0 39.0 35.0 39.0 14-15 39.28110538238989 41.0 39.0 41.0 36.0 41.0 16-17 39.209359375895076 40.5 39.0 41.0 36.0 41.0 18-19 39.0898649412392 40.0 38.5 41.0 36.0 41.0 20-21 38.793652004315305 40.0 38.0 41.0 34.0 41.0 22-23 38.53702966016179 40.0 38.0 41.0 34.0 41.0 24-25 38.435662479701 40.0 38.0 41.0 34.0 41.0 26-27 38.24120228833399 40.0 38.0 41.0 33.5 41.0 28-29 38.118367225834035 40.0 38.0 41.0 33.0 41.0 30-31 37.98085998315357 40.0 38.0 41.0 33.0 41.0 32-33 37.90216671344183 40.0 38.0 41.0 33.0 41.0 34-35 37.94124091698643 40.0 38.0 41.0 32.5 41.0 36-37 37.90098811435997 40.0 38.0 41.0 33.0 41.0 38-39 37.60971169695735 40.0 37.0 41.0 32.5 41.0 40-41 37.48535719883911 40.0 36.5 41.0 32.0 41.0 42-43 37.31406101368485 40.0 36.0 41.0 31.0 41.0 44-45 37.14381848235659 40.0 36.0 41.0 31.0 41.0 46-47 36.93908022363421 40.0 35.0 41.0 30.0 41.0 48-49 36.725646634677815 39.0 35.0 41.0 30.0 41.0 50-51 36.72490987210579 39.0 35.0 41.0 30.0 41.0 52-53 36.46705534362616 39.0 35.0 41.0 29.5 41.0 54-55 36.04555330579053 39.0 35.0 41.0 27.0 41.0 56-57 35.57271792402015 39.0 35.0 41.0 25.5 41.0 58-59 35.19291352566471 38.0 34.0 40.5 24.0 41.0 60-61 34.95796731075997 38.0 34.0 40.0 23.5 41.0 62-63 34.74784606044538 37.5 34.0 40.0 23.0 41.0 64-65 34.4860951740248 37.0 34.0 40.0 23.0 41.0 66-67 34.13013280176443 36.5 33.5 39.5 23.0 41.0 68-69 33.80050560463895 36.0 33.0 39.0 22.0 41.0 70-71 33.46923522390343 35.5 33.0 39.0 22.0 41.0 72-73 32.96247058951382 35.0 32.0 37.5 21.5 40.0 74-75 32.57691746607583 35.0 32.0 37.0 20.0 39.5 76-77 30.83292449321805 33.5 29.5 35.5 19.0 38.5 78-79 31.93889114800008 35.0 31.5 36.0 20.0 39.0 80-81 31.875676308408607 35.0 32.0 36.0 20.0 38.0 82-83 31.66729353164857 35.0 32.0 35.5 20.0 37.0 84-85 31.365673013169353 35.0 31.5 35.0 19.0 37.0 86-87 31.186030991458104 35.0 31.0 35.0 18.0 36.0 88-89 30.842654182906223 35.0 31.0 35.0 16.0 36.0 90-91 30.307269947387304 35.0 30.5 35.0 11.5 36.0 92-93 29.791566892098196 35.0 29.5 35.0 10.0 35.5 94-95 29.319900454549 34.5 29.0 35.0 8.0 35.0 96-97 28.98596430384648 34.0 28.5 35.0 7.5 35.0 98-99 28.78862232843558 34.0 27.0 35.0 7.0 35.0 100 28.638057232049373 34.0 27.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17725765452424014 1101 2 0.18387013638668037 1101 3 0.22202025978475604 1101 4 0.14498452698011022 1101 5 0.11612928741165263 1101 6 0.14784541767626536 1101 7 0.18517912857966934 1101 8 0.18179039472660463 1101 9 0.24471624970862393 1101 10-11 0.2587886208066337 1101 12-13 0.25948948114946546 1101 14-15 0.3228025320299537 1101 16-17 0.2811218877172337 1101 18-19 0.2960664290140329 1101 20-21 0.33058579427395784 1101 22-23 0.2750119676662166 1101 24-25 0.244343371742886 1101 26-27 0.2731478033774337 1101 28-29 0.31689850366246475 1101 30-31 0.31414679683790325 1101 32-33 0.34291553000807795 1101 34-35 0.34613454860193116 1101 36-37 0.34588032565822857 1101 38-39 0.3401425544913579 1101 40-41 0.3298458818380112 1101 42-43 0.34545613303884437 1101 44-45 0.3637277706172597 1101 46-47 0.34912316808252797 1101 48-49 0.2687473757797676 1101 50-51 0.3114344879402253 1101 52-53 0.36424483589956225 1101 54-55 0.3233618765738058 1101 56-57 0.23781467748890606 1101 58-59 0.24599168137373795 1101 60-61 0.15107862236378367 1101 62-63 0.05765103120054249 1101 64-65 0.032390724762485945 1101 66-67 -0.12427667804298892 1101 68-69 -0.16489990005050004 1101 70-71 -0.2190957079824969 1101 72-73 -0.25303353117296723 1101 74-75 -0.29161359653240027 1101 76-77 -0.27797795292316607 1101 78-79 -0.28450994569558574 1101 80-81 -0.23502100868772047 1101 82-83 -0.19977698674376754 1101 84-85 -0.15413786670667307 1101 86-87 -0.10751948170319992 1101 88-89 -0.13021729941803883 1101 90-91 -0.11611364432972948 1101 92-93 -0.21402304400886507 1101 94-95 -0.02379375141581974 1101 96-97 0.055103418430199724 1101 98-99 0.02693718212787033 1101 100 0.04104517543847663 1102 1 0.13362300496169865 1102 2 0.14158848348577635 1102 3 0.18251519955213524 1102 4 0.11153545763925621 1102 5 0.10400227909487825 1102 6 0.14529325293930384 1102 7 0.14925832766672187 1102 8 0.17466646114286988 1102 9 0.2213591205601375 1102 10-11 0.21736859058999158 1102 12-13 0.23129884567230619 1102 14-15 0.23142947082524046 1102 16-17 0.2192790425566571 1102 18-19 0.2532046631655902 1102 20-21 0.29336386994059893 1102 22-23 0.20957609453809312 1102 24-25 0.22629517068203597 1102 26-27 0.26888106364504694 1102 28-29 0.2594427531571881 1102 30-31 0.23286395443155783 1102 32-33 0.28195939296583816 1102 34-35 0.27970788166643956 1102 36-37 0.2737403932548048 1102 38-39 0.31762483792335416 1102 40-41 0.27515533046505425 1102 42-43 0.29642523981796387 1102 44-45 0.26911795610998723 1102 46-47 0.2850902659346133 1102 48-49 0.23906329944777127 1102 50-51 0.24966477824840894 1102 52-53 0.33166868133397287 1102 54-55 0.23465666539109975 1102 56-57 0.2440741162282336 1102 58-59 0.29394510875795277 1102 60-61 0.17856115306783948 1102 62-63 0.09441514398425 1102 64-65 0.07474189598564607 1102 66-67 -0.01711307314342747 1102 68-69 -0.07274552569035819 1102 70-71 -0.03782138061165341 1102 72-73 -0.1235399050542938 1102 74-75 -0.2065991935685787 1102 76-77 -0.3310280887294219 1102 78-79 -0.11577028951407797 1102 80-81 -0.05585861935227143 1102 82-83 -0.031909430607775846 1102 84-85 0.010086582528089139 1102 86-87 -0.0664981111197882 1102 88-89 -0.08369460088853131 1102 90-91 -0.053716763914117394 1102 92-93 -0.11583501566692078 1102 94-95 -0.00638825483676797 1102 96-97 0.07863332100944476 1102 98-99 0.07698000140730343 1102 100 0.0727149700967793 1103 1 0.13389573106650232 1103 2 0.13078377541648933 1103 3 0.13765257613972182 1103 4 0.09021780981881733 1103 5 0.09770628626085198 1103 6 0.12403276720787204 1103 7 0.14521504529520257 1103 8 0.1339582795418579 1103 9 0.1602455999371415 1103 10-11 0.20765941608753735 1103 12-13 0.2184823121171604 1103 14-15 0.2402878618985369 1103 16-17 0.2287937256274546 1103 18-19 0.2102529022699855 1103 20-21 0.2972076179576746 1103 22-23 0.2765000229982917 1103 24-25 0.2609178230902458 1103 26-27 0.23056976289085895 1103 28-29 0.29081506000202495 1103 30-31 0.2676464163354808 1103 32-33 0.31357334877394294 1103 34-35 0.3027397467409756 1103 36-37 0.2953768056240733 1103 38-39 0.2657092454126868 1103 40-41 0.2596736773100119 1103 42-43 0.3219697663813932 1103 44-45 0.2943658181179174 1103 46-47 0.28596336082858187 1103 48-49 0.2483261988145884 1103 50-51 0.26746082186615894 1103 52-53 0.3251103795053112 1103 54-55 0.2614784754900157 1103 56-57 0.24814488500424403 1103 58-59 0.23689996227133747 1103 60-61 0.19687694655221577 1103 62-63 0.16528055879590653 1103 64-65 0.13699637437428436 1103 66-67 0.1442584708149468 1103 68-69 0.02552844398906018 1103 70-71 0.05691231058766988 1103 72-73 0.053108226441658246 1103 74-75 0.05772794136417758 1103 76-77 -0.10894444104616241 1103 78-79 0.07390768059722319 1103 80-81 0.1458818704844056 1103 82-83 0.14541860007177476 1103 84-85 0.12861635154731133 1103 86-87 0.10085511058177943 1103 88-89 0.07939065792635702 1103 90-91 0.044673664216276165 1103 92-93 -0.008645350418102993 1103 94-95 0.15980815362675216 1103 96-97 0.14744832656693063 1103 98-99 0.17449868048253236 1103 100 0.17991850200807846 1104 1 0.11777970320689946 1104 2 0.1217893253243929 1104 3 0.14794960204312702 1104 4 0.10455976507012821 1104 5 0.07242973334479785 1104 6 0.10458275654281834 1104 7 0.10204946727917275 1104 8 0.1190416372697598 1104 9 0.18724493966239208 1104 10-11 0.20628952976074544 1104 12-13 0.1835895383181665 1104 14-15 0.22242063785951416 1104 16-17 0.1825102524725679 1104 18-19 0.1503730703793167 1104 20-21 0.23395352552973492 1104 22-23 0.17944977245358018 1104 24-25 0.1633782570178255 1104 26-27 0.19072558818829322 1104 28-29 0.1956193010847329 1104 30-31 0.18638527024099005 1104 32-33 0.22031787271750858 1104 34-35 0.1807234812046019 1104 36-37 0.18121432083678002 1104 38-39 0.238435977680858 1104 40-41 0.2140287645611565 1104 42-43 0.2099898303924519 1104 44-45 0.24207720028960722 1104 46-47 0.22042166829821497 1104 48-49 0.23438118093996962 1104 50-51 0.2591798934179721 1104 52-53 0.28350782620631065 1104 54-55 0.22570088607944427 1104 56-57 0.2221985062272367 1104 58-59 0.2507755061752306 1104 60-61 0.178801349166001 1104 62-63 0.19380759798033154 1104 64-65 0.1680928039947318 1104 66-67 0.15819601430440144 1104 68-69 0.1404289277597286 1104 70-71 0.1028311432489275 1104 72-73 0.10920892350936384 1104 74-75 0.08384743876538181 1104 76-77 0.07901892089607543 1104 78-79 0.16877139076289893 1104 80-81 0.23592900949136109 1104 82-83 0.2671376992478187 1104 84-85 0.21573750175778272 1104 86-87 0.21646434101592504 1104 88-89 0.1595042396037769 1104 90-91 0.14402318318345309 1104 92-93 0.03855386673903283 1104 94-95 0.1353670974057941 1104 96-97 0.1889467894551089 1104 98-99 0.13986817272977348 1104 100 0.24838165811737412 1105 1 0.05842472127050513 1105 2 0.07186256881395536 1105 3 0.07764661511108528 1105 4 0.09409592310760218 1105 5 0.071677428365156 1105 6 0.08444228568728107 1105 7 0.08584293964885603 1105 8 0.10594928413546967 1105 9 0.12314725448681685 1105 10-11 0.1827408410547875 1105 12-13 0.14667045732571893 1105 14-15 0.16793848858120697 1105 16-17 0.15972966567136382 1105 18-19 0.16006923870000378 1105 20-21 0.2337628838198782 1105 22-23 0.1835734081913074 1105 24-25 0.1641941527366768 1105 26-27 0.1832627414123067 1105 28-29 0.17725391592120587 1105 30-31 0.1330780200101458 1105 32-33 0.1694574136360174 1105 34-35 0.14719887471577664 1105 36-37 0.16842724130633968 1105 38-39 0.1827844024566403 1105 40-41 0.24115090548468032 1105 42-43 0.28273509334326974 1105 44-45 0.22140325803856342 1105 46-47 0.2410036420444257 1105 48-49 0.19778327192160816 1105 50-51 0.21218425011208808 1105 52-53 0.20029315881003384 1105 54-55 0.19277980021548302 1105 56-57 0.1708409682290366 1105 58-59 0.20450014518747395 1105 60-61 0.1048776859400462 1105 62-63 0.14214483847171522 1105 64-65 0.1740961202830178 1105 66-67 0.1464692660846154 1105 68-69 0.1459457614858266 1105 70-71 0.1408292252934089 1105 72-73 0.10609630797814873 1105 74-75 0.1023825652013528 1105 76-77 -0.012773571156184715 1105 78-79 0.21234498897371168 1105 80-81 0.2545917007038305 1105 82-83 0.26089159406886964 1105 84-85 0.2660810946397767 1105 86-87 0.17343667933883467 1105 88-89 0.06839703568849842 1105 90-91 0.024923401888393926 1105 92-93 -0.02406405869431083 1105 94-95 0.13038056669720888 1105 96-97 0.2163187963005342 1105 98-99 0.22277226560318297 1105 100 0.21228590356205856 1106 1 0.03657479008845144 1106 2 0.06871932268728642 1106 3 0.09463657675646431 1106 4 0.06381625000224744 1106 5 0.03938160277817815 1106 6 0.04488155953891493 1106 7 0.05541692948987276 1106 8 0.057992833847428926 1106 9 0.07087300345099834 1106 10-11 0.14125045318450447 1106 12-13 0.11379169958664193 1106 14-15 0.11927978894487978 1106 16-17 0.12463360965063686 1106 18-19 0.11940521091713663 1106 20-21 0.16698318675931745 1106 22-23 0.10363358539296996 1106 24-25 0.09438114821067956 1106 26-27 0.09862525018262858 1106 28-29 0.12548312349491653 1106 30-31 0.08049400620452474 1106 32-33 0.07757139622058418 1106 34-35 0.05287640536791827 1106 36-37 0.08345601125012081 1106 38-39 0.12851212357845299 1106 40-41 0.1628715333224804 1106 42-43 0.13181304717317488 1106 44-45 0.19516125956131702 1106 46-47 0.12405208953516222 1106 48-49 0.1439473477869413 1106 50-51 0.11628462953733276 1106 52-53 0.1401105906009903 1106 54-55 0.0959893455144254 1106 56-57 0.09415194491744927 1106 58-59 0.09425198135268431 1106 60-61 0.04242401018322539 1106 62-63 0.05716300881490355 1106 64-65 0.05030523883046811 1106 66-67 0.009471560693249614 1106 68-69 0.09602590645808107 1106 70-71 0.04454506862268914 1106 72-73 0.07990088413553309 1106 74-75 0.056109623460741886 1106 76-77 -0.07148877299325562 1106 78-79 0.16342879881340622 1106 80-81 0.18314895310081525 1106 82-83 0.16053953539942611 1106 84-85 0.12680323424026696 1106 86-87 0.09146546159337987 1106 88-89 0.009511294984687169 1106 90-91 -0.03077344349883049 1106 92-93 -0.10039558036594443 1106 94-95 -0.02437615324050313 1106 96-97 0.02699241329650448 1106 98-99 0.05651843886842656 1106 100 0.06836191757016508 1107 1 -0.00783593660317905 1107 2 0.030894405744916753 1107 3 0.04183253749938132 1107 4 0.03246643947550609 1107 5 0.007368581261815166 1107 6 0.015685062893155077 1107 7 0.055072259605054796 1107 8 0.0432932818841536 1107 9 0.043099048291850295 1107 10-11 0.06550434000746463 1107 12-13 0.06062667265619126 1107 14-15 0.050838950169278974 1107 16-17 0.034483509135171175 1107 18-19 0.06280107344282726 1107 20-21 0.1384142340510266 1107 22-23 0.08314409067655504 1107 24-25 0.03343623092002446 1107 26-27 0.022141419391914496 1107 28-29 -4.384066981302226E-4 1107 30-31 -0.010202604607556509 1107 32-33 0.01137867112824864 1107 34-35 -0.008083559815695196 1107 36-37 -0.016302678602045262 1107 38-39 -6.005102470183488E-4 1107 40-41 0.005912841391925383 1107 42-43 0.02759009137728441 1107 44-45 0.02101175864961391 1107 46-47 0.037574905836649464 1107 48-49 0.018795166210942682 1107 50-51 0.007914252913792552 1107 52-53 0.06458136424921435 1107 54-55 -0.0480705271227464 1107 56-57 -0.08399940190857791 1107 58-59 -0.10085511209435083 1107 60-61 -0.11860556017266077 1107 62-63 -0.10201873818336793 1107 64-65 -0.11554645996643131 1107 66-67 -0.11200046393481244 1107 68-69 -0.09639634363409755 1107 70-71 -0.07893821122764422 1107 72-73 -0.08153884277175649 1107 74-75 -0.06959481475109186 1107 76-77 -0.1842760876612175 1107 78-79 0.04040276866803083 1107 80-81 0.05587164883294449 1107 82-83 0.06014994711275534 1107 84-85 0.04033966826102642 1107 86-87 -0.038539402358271246 1107 88-89 -0.12016729999264797 1107 90-91 -0.12171218518116689 1107 92-93 -0.14991997739007346 1107 94-95 -0.06141468326562105 1107 96-97 -0.04559260425094891 1107 98-99 -0.10286783825448609 1107 100 -0.05869936783991747 1108 1 -0.0285775227092131 1108 2 -0.0190204655070616 1108 3 -0.03378600959140954 1108 4 0.006020091691411267 1108 5 -0.022492630163561955 1108 6 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-0.695829468156866 2316 76-77 -0.6108889414739984 2316 78-79 -0.6409557121723779 2316 80-81 -0.538207868539228 2316 82-83 -0.5099322605343879 2316 84-85 -0.5260267717538376 2316 86-87 -0.496405489564129 2316 88-89 -0.5892334356278646 2316 90-91 -1.0167088152127874 2316 92-93 -0.9375690720032388 2316 94-95 -0.9016959822003194 2316 96-97 -1.8036332067736254 2316 98-99 -1.903722541112817 2316 100 -1.7991839933330773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 23.0 9 55.0 10 254.0 11 668.0 12 920.0 13 1613.0 14 3151.0 15 8602.0 16 26282.0 17 62295.0 18 110230.0 19 162566.0 20 206834.0 21 235419.0 22 273269.0 23 346568.0 24 477570.0 25 614344.0 26 648081.0 27 603757.0 28 587458.0 29 632392.0 30 720451.0 31 838428.0 32 1006198.0 33 1268569.0 34 1596080.0 35 2028624.0 36 3335901.0 37 6677600.0 38 8003016.0 39 2093466.0 40 3581.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.451283196014245 48.53579809303092 14.882613520797856 18.13030519015698 2 19.454672419820948 30.473666940799877 35.22331336544912 14.848347273930054 3 23.513969585684602 36.76321363618754 21.071019681610018 18.651797096517846 4 18.028264700284275 24.084069601095344 34.61072873623911 23.27693696238127 5 18.887916000931533 41.74459372315557 22.00227934529668 17.36521093061621 6 23.558154771622483 22.855287053897086 35.513018159795216 18.073540014685214 7 18.63649360510533 36.1312086049767 27.08981071131426 18.142487078603704 8 22.846193986381767 22.556815247962916 36.230437241471535 18.366553524183786 9 22.821537351459387 34.746027660616406 23.990939347931693 18.441495639992514 10-11 18.135461594130778 31.961418562739063 31.89076616492295 18.01235367820721 12-13 18.314116425524368 29.593059748452966 31.27408304457267 20.818740781449996 14-15 18.64600878497155 34.50761714784302 28.3686315449134 18.477742522272028 16-17 22.04603320915965 28.428933747885527 28.03514436825683 21.489888674697998 18-19 20.72726673257964 30.679517076455383 26.821285857983725 21.771930332981256 20-21 24.343505334346055 30.18652793289568 25.785227537105897 19.68473919565237 22-23 25.201300007803706 26.957539119991225 27.81559375735435 20.025567114850723 24-25 23.378127647955566 30.454131104632697 23.7655624687802 22.402178778631537 26-27 21.946613916897558 29.69670684019226 28.067618560636742 20.28906068227344 28-29 24.094331720563503 27.429215844309425 27.22164390631101 21.25480852881606 30-31 23.15925569978645 26.522802439035587 27.43679058052031 22.881151280657654 32-33 21.48150482887534 27.60450534443254 27.74727420299695 23.16671562369517 34-35 22.15629497038731 26.86689312620873 28.65173226567066 22.3250796377333 36-37 22.066824801734295 25.70021401745401 29.87386598241828 22.359095198393423 38-39 21.550200785704952 26.19340647762195 29.153730382358745 23.102662354314354 40-41 21.761629232153958 27.45120167132032 27.94236276858984 22.84480632793589 42-43 20.653381588360055 26.565525975948635 28.86627831430661 23.9148141213847 44-45 20.506480268149343 26.04209010167352 29.27213196489424 24.179297665282892 46-47 21.424014857056843 26.28735634001264 28.91815738146921 23.370471421461303 48-49 21.674420495245208 25.68422188881907 29.76205303914089 22.87930457679483 50-51 21.616939383094298 25.602169102841454 29.055414278864767 23.725477235199484 52-53 22.329386751201678 24.721622406051562 29.900989880134297 23.048000962612463 54-55 21.514993568724925 25.123145910519497 30.876826209849707 22.485034310905874 56-57 22.935930510508108 25.49429962247115 28.579876835754707 22.989893031266035 58-59 23.708276916253347 27.216135186364625 27.086603438597194 21.988984458784834 60-61 21.72060132303004 27.871333311349765 27.880519740660226 22.52754562495997 62-63 21.37612480687135 25.941796346838885 29.214478436124146 23.467600410165616 64-65 22.478944746178314 25.667509017539693 28.96402816624004 22.88951807004195 66-67 22.721905152645977 26.236093794655428 28.925459762740154 22.116541289958437 68-69 22.256742503777993 26.196151780205884 28.794701256007205 22.752404460008915 70-71 23.050217746622184 24.801458007569977 28.12335928633229 24.024964959475554 72-73 22.332966573919418 24.507436406958565 28.997376613819835 24.162220405302183 74-75 21.236992713365183 24.359037767796355 30.9819165927393 23.42205292609916 76-77 21.150037494775507 24.474282599654963 31.476581591523512 22.899098314046025 78-79 22.017440347026298 25.21330175374534 29.18814502422395 23.581112875004408 80-81 22.158994008512515 26.594616641356104 28.836744822131983 22.409644527999394 82-83 21.70894435736481 28.68189166714291 28.674766062738687 20.934397912753592 84-85 21.813851140030565 31.738287108443288 25.851560577806982 20.596301173719166 86-87 20.557674979084638 33.98337209391574 25.172806189412604 20.286146737587018 88-89 20.415754748075916 34.84003396500198 24.65100463116695 20.093206655755164 90-91 20.457188556394307 35.019470370016464 24.25057092541916 20.272770148170064 92-93 20.482575496929076 35.09815413559237 24.008745967685115 20.410524399793438 94-95 20.75898975168063 34.52405351387089 23.946099987425377 20.770856747023107 96-97 21.023038410223688 33.633999957726815 24.031754867836355 21.311206764213143 98-99 21.247452635110974 33.16167805390801 24.066670754781345 21.524198556199668 100 21.58931632415894 32.50846446841965 24.079748371920065 21.82247083550134 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5062.0 1 5134.0 2 4598.5 3 4081.0 4 4363.0 5 4928.5 6 5563.0 7 5914.5 8 6139.5 9 6435.5 10 6898.5 11 9122.5 12 68375.0 13 86161.0 14 47397.0 15 52191.5 16 60511.5 17 69487.0 18 78481.5 19 87407.0 20 97939.0 21 112883.5 22 132785.0 23 156329.5 24 181360.0 25 208530.5 26 239137.5 27 276125.5 28 324496.5 29 376993.0 30 426643.0 31 477531.5 32 537883.0 33 629472.5 34 1054451.0 35 1465428.0 36 1483986.5 37 1375599.0 38 1224076.5 39 1092100.5 40 991082.0 41 930104.0 42 910020.0 43 903199.5 44 902194.0 45 923977.0 46 943819.5 47 955373.5 48 960413.5 49 960523.0 50 962038.0 51 965732.0 52 966699.0 53 944555.5 54 903815.5 55 866770.5 56 826899.5 57 770058.5 58 695465.0 59 606228.0 60 501922.0 61 405051.5 62 322871.5 63 248100.5 64 185991.0 65 137392.5 66 100370.0 67 73902.0 68 54868.5 69 41402.5 70 31621.5 71 23768.5 72 18200.0 73 13708.0 74 10611.5 75 8914.0 76 6741.0 77 4031.5 78 2289.0 79 1472.0 80 944.0 81 597.5 82 355.0 83 223.0 84 149.5 85 100.0 86 75.0 87 50.0 88 33.0 89 23.0 90 16.5 91 10.0 92 5.5 93 5.5 94 4.5 95 3.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10064386408584003 2 0.003972460960440368 3 0.0 4 2.0261392843049785E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 3.069908006522695E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.05622861924195E-5 22-23 0.0 24-25 0.002416017601133361 26-27 0.013483035964647675 28-29 0.02149703081567517 30-31 0.0231517112311909 32-33 0.0202291588089813 34-35 0.009760772506738909 36-37 0.005185074623016831 38-39 0.004540393941647066 40-41 0.0022640571548104877 42-43 0.004161260302841513 44-45 0.00650360011181833 46-47 0.006141350967048651 48-49 0.0053876885514473295 50-51 0.008113766861239482 52-53 0.014014130049776101 54-55 0.017453961971084783 56-57 0.019314326223037536 58-59 0.018998125698365696 60-61 0.013765467501247762 62-63 0.01230419129014296 64-65 0.011400103382222027 66-67 0.011326425590065482 68-69 0.009441502074060549 70-71 0.008267262261565617 72-73 0.006294846367374786 74-75 0.0053846186434408074 76-77 0.005304801035271217 78-79 0.005369269103408193 80-81 0.006818265682486905 82-83 0.004882688684374347 84-85 0.007429177375784921 86-87 0.0077238885444110995 88-89 0.005875803924484438 90-91 0.0032341480848716592 92-93 0.002624771345576904 94-95 0.0022410328447615676 96-97 0.0013062458567754068 98-99 5.019299590664606E-4 100 4.881153730371085E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.2574266E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.69827973287971 #Duplication Level Percentage of deduplicated Percentage of total 1 72.16622743538952 26.483764016905432 2 13.072099388353408 9.594471200995983 3 4.994198907340689 5.498355256296923 4 2.6001503003831528 3.816841722839686 5 1.6148822099752207 2.963169953866082 6 1.0860279599223035 2.391321472257442 7 0.7709144280109149 1.9803863330480262 8 0.5825076225968073 1.7101622144473887 9 0.4260293807153853 1.4071090849126848 >10 2.3721131281537295 15.686345359530614 >50 0.20809249149026252 5.333098209362005 >100 0.10271491987187296 6.291160179053409 >500 0.0021130677857528718 0.512363575838721 >1k 0.0015734112242805113 1.157855061205044 >5k 1.3537096643336573E-4 0.3246634818533259 >10k+ 2.1997782040859232E-4 14.848932877587245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1166788 3.5819318231146022 No Hit ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC 933137 2.864644747482568 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp) ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 781696 2.3997348090667647 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp) ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT 310361 0.9527797188123901 TruSeq Adapter, Index 1 (97% over 35bp) ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG 307204 0.943088019235798 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp) ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT 246319 0.7561766702586636 TruSeq Adapter, Index 21 (100% over 37bp) ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC 204589 0.6280694091464717 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp) ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT 139856 0.429345054160238 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp) ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG 111330 0.3417728583661716 TruSeq Adapter, Index 21 (100% over 39bp) ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA 92073 0.2826556398845641 Illumina Multiplexing PCR Primer 2.01 (100% over 24bp) TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT 60649 0.1861868506875949 TruSeq Adapter, Index 9 (97% over 36bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT 54548 0.16745734193979997 TruSeq Adapter, Index 21 (97% over 40bp) ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG 46757 0.14353968866098163 Illumina Multiplexing PCR Primer 2.01 (100% over 22bp) ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT 46689 0.14333093491653812 TruSeq Adapter, Index 9 (97% over 36bp) TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC 36229 0.11121969716831072 TruSeq Adapter, Index 21 (100% over 38bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.22340340684883E-4 1.2279632026090779E-5 6.1398160130453896E-6 3.0699080065226948E-6 0.0 2 4.2057739689360923E-4 2.1489356045658865E-5 6.1398160130453896E-6 3.0699080065226948E-6 0.0 3 5.771427052262666E-4 2.1489356045658865E-5 6.1398160130453896E-6 6.1398160130453896E-6 3.0699080065226948E-6 4 6.385408653567206E-4 2.7629172058704255E-5 6.1398160130453896E-6 6.1398160130453896E-6 9.209724019568086E-6 5 9.486015740155127E-4 2.7629172058704255E-5 6.1398160130453896E-6 6.1398160130453896E-6 1.2279632026090779E-5 6 0.001065258078263375 3.376898807174964E-5 6.1398160130453896E-6 6.1398160130453896E-6 2.7629172058704255E-5 7 0.0013538294308765085 3.683889607827234E-5 6.1398160130453896E-6 6.1398160130453896E-6 3.069908006522695E-5 8 0.00153802391126787 3.683889607827234E-5 6.1398160130453896E-6 6.1398160130453896E-6 3.069908006522695E-5 9 0.045760048745227294 4.297871209131773E-5 6.1398160130453896E-6 6.1398160130453896E-6 3.376898807174964E-5 10-11 0.2513164839999772 4.604862009784042E-5 6.1398160130453896E-6 6.1398160130453896E-6 3.376898807174964E-5 12-13 1.0444210776691023 5.218843611088582E-5 6.1398160130453896E-6 6.1398160130453896E-6 3.9908804084795036E-5 14-15 2.311140333906526 5.525834411740851E-5 6.1398160130453896E-6 6.1398160130453896E-6 4.297871209131773E-5 16-17 4.320408938761659 6.600302214023794E-5 6.1398160130453896E-6 1.2279632026090779E-5 4.604862009784042E-5 18-19 7.349286089823175 8.442247017937411E-5 6.1398160130453896E-6 1.3814586029352128E-5 4.911852810436312E-5 20-21 9.537631638422798 1.0284191821851029E-4 6.1398160130453896E-6 1.5349540032613474E-5 5.3723390114147163E-5 22-23 10.468283460324171 1.1665650424786241E-4 6.1398160130453896E-6 1.5349540032613474E-5 5.8328252123931204E-5 24-25 11.11297028150995 1.2740118227069183E-4 6.1398160130453896E-6 1.5349540032613474E-5 7.060788415002198E-5 26-27 11.558174480431884 1.396808142967826E-4 9.209724019568086E-6 1.688449403587482E-5 7.060788415002198E-5 28-29 11.830921992225397 1.4428567630656665E-4 9.209724019568086E-6 1.841944803913617E-5 7.521274615980603E-5 30-31 11.984807270868359 1.519604463228734E-4 9.209724019568086E-6 1.841944803913617E-5 7.674770016306738E-5 32-33 12.078533097261502 1.7037989436200957E-4 9.209724019568086E-6 1.841944803913617E-5 8.74923781858968E-5 34-35 12.134294906291979 1.841944803913617E-4 9.209724019568086E-6 1.9954402042397518E-5 9.05622861924195E-5 36-37 12.166990961515449 2.0261392843049785E-4 9.209724019568086E-6 2.4559264052181558E-5 9.363219419894219E-5 38-39 12.191112763676701 2.0568383643702057E-4 9.209724019568086E-6 2.4559264052181558E-5 9.516714820220354E-5 40-41 12.214915295405275 2.133586064533273E-4 9.209724019568086E-6 3.376898807174964E-5 9.670210220546489E-5 42-43 12.242842248540612 2.2410328447615673E-4 1.2279632026090779E-5 3.6838896078272336E-5 1.0130696421524893E-4 44-45 12.275702543842431 2.3331300849572482E-4 1.2279632026090779E-5 3.9908804084795036E-5 1.0284191821851029E-4 46-47 12.313559114424866 2.4405768651855424E-4 1.2279632026090779E-5 3.9908804084795036E-5 1.0898173423155567E-4 48-49 12.357976078417238 2.5480236454138367E-4 1.2279632026090779E-5 4.4513666094579076E-5 1.1819145825112375E-4 50-51 12.42206961777742 2.594072265511677E-4 1.2279632026090779E-5 4.604862009784042E-5 1.258662282674305E-4 52-53 12.495268504284947 2.655470425642131E-4 1.2279632026090779E-5 4.604862009784042E-5 1.3200604428047588E-4 54-55 12.568556418124663 2.686169505707358E-4 1.2279632026090779E-5 4.9118528104363116E-5 1.5042549231961206E-4 56-57 12.655388766089157 2.747567665837812E-4 1.2279632026090779E-5 5.3723390114147163E-5 1.6577503235222553E-4 58-59 12.756393344365765 2.8396649060334926E-4 1.2279632026090779E-5 5.525834411740851E-5 1.7037989436200957E-4 60-61 12.874029149267708 2.9164126061965605E-4 1.2279632026090779E-5 5.679329812066986E-5 1.780546643783163E-4 62-63 13.009952396164508 3.023859386424855E-4 1.3814586029352128E-5 6.293311413371524E-5 1.841944803913617E-4 64-65 13.167920652456145 3.0852575465553086E-4 1.5349540032613474E-5 7.060788415002198E-5 1.9647411241745247E-4 66-67 13.349000711174888 3.192704326783603E-4 1.5349540032613474E-5 8.288751617611277E-5 2.1028869844680461E-4 68-69 13.553439699915264 3.330850187077124E-4 1.5349540032613474E-5 9.209724019568085E-5 2.1642851445985E-4 70-71 13.78552935007039 3.453646507338032E-4 1.5349540032613474E-5 9.823705620872623E-5 2.194984224663727E-4 72-73 14.047905177663864 3.62249144769678E-4 1.5349540032613474E-5 1.0130696421524894E-4 2.3024310048920212E-4 74-75 14.340366103721262 3.714588687892461E-4 1.5349540032613474E-5 1.0744678022829433E-4 2.3484796249898616E-4 76-77 14.665745653332603 3.8373850081533685E-4 1.5349540032613474E-5 1.1358659624133971E-4 2.4712759452507694E-4 78-79 15.025945634507927 3.883433628251209E-4 1.5349540032613474E-5 1.1665650424786241E-4 2.56337318544645E-4 80-81 15.421481791792331 4.036929028577344E-4 1.5349540032613474E-5 1.197264122543851E-4 2.6401208856095175E-4 82-83 15.854002665785316 4.159725348838252E-4 1.5349540032613474E-5 1.197264122543851E-4 2.686169505707358E-4 84-85 16.321626402878884 4.236473049001319E-4 1.5349540032613474E-5 1.197264122543851E-4 2.762917205870425E-4 86-87 16.828360767975553 4.297871209131773E-4 1.5349540032613474E-5 1.197264122543851E-4 2.8396649060334926E-4 88 17.230927014594894 4.328570289197E-4 1.5349540032613474E-5 1.197264122543851E-4 2.9164126061965605E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 183865 0.0 85.736664 8 TGGTATC 186180 0.0 84.599915 7 GTATCAA 186705 0.0 84.37965 9 AGTGGTA 187210 0.0 84.3152 5 GTGGTAT 187455 0.0 84.1323 6 AAGCAGT 196555 0.0 81.481415 1 GCAGTGG 198550 0.0 80.363754 3 CAGTGGT 200860 0.0 79.15176 4 AGCAGTG 207335 0.0 77.22537 2 CAACGCA 181610 0.0 45.218407 12-13 ATCAACG 182760 0.0 44.81045 10-11 ACGCAGA 184705 0.0 44.341267 14-15 TCAACGC 181305 0.0 43.715973 12-13 AGAGTAC 181935 0.0 43.67038 18-19 AACGCAG 183470 0.0 42.986374 14-15 CGCAGAG 186340 0.0 42.224678 16-17 TATCAAC 187095 0.0 42.139557 10-11 GAGTACT 147215 0.0 41.608322 20-21 AGTACTT 156000 0.0 41.15576 20-21 GCAGAGT 200895 0.0 40.365486 16-17 >>END_MODULE