FastQCFastQC Report
Thu 26 May 2016
SRR1660593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25946227
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG7893003.0420607975101737Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA6166312.376572902102491Illumina Single End PCR Primer 1 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3772581.453999458187119No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2662291.0260798227040873Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA2468080.9512288626781844Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2137470.8238076387753794Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1710140.6591093186689533Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1077250.4151856067550785Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC1062450.40948150187693955Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG725080.2794548895297956Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT448400.17281896130793892Illumina Single End PCR Primer 1 (100% over 37bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT377970.1456743595128494Illumina Single End PCR Primer 1 (100% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG373980.1441365636707025Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT307070.1183486138466298Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA926750.081.746348
GTGGTAT932350.081.308246
TGGTATC933250.081.011797
AGTGGTA941450.080.675155
GTATCAA960250.078.84379
GCAGTGG1030000.074.503243
AAGCAGT1040900.074.0455861
CAGTGGT1028250.074.016874
AGCAGTG1118300.068.875292
CAACGCA889950.044.27065712-13
ATCAACG896800.043.81609710-11
ACGCAGA909400.043.32815614-15
TCAACGC877350.043.32387512-13
AACGCAG905300.042.14875414-15
AGAGTAC897800.041.84687818-19
CGCAGAG922650.041.20933516-17
TATCAAC939650.040.30038510-11
CGTACCG23250.039.2059363
GAGTACT560000.038.69869620-21
CAGAGTA972350.037.9280118-19
Produced by FastQC (version 0.11.5)