##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660593_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25946227 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856051247836536 34.0 31.0 34.0 30.0 34.0 2 31.96266250965892 34.0 31.0 34.0 30.0 34.0 3 31.960611036047744 34.0 31.0 34.0 30.0 34.0 4 35.350722553996 37.0 35.0 37.0 33.0 37.0 5 35.34604661402215 37.0 35.0 37.0 33.0 37.0 6 35.34089715626091 37.0 35.0 37.0 33.0 37.0 7 35.26135117834281 37.0 35.0 37.0 33.0 37.0 8 35.27318862199117 37.0 35.0 37.0 33.0 37.0 9 36.91958167944804 39.0 37.0 39.0 33.0 39.0 10-11 36.90150448464048 39.0 37.0 39.0 33.0 39.0 12-13 36.888236621070185 39.0 37.0 39.0 33.0 39.0 14-15 38.29924451057951 40.0 38.0 41.0 33.0 41.0 16-17 38.237114378903726 40.0 38.0 41.0 33.0 41.0 18-19 38.170193859014645 40.0 38.0 41.0 33.0 41.0 20-21 38.04649992077846 40.0 38.0 41.0 32.0 41.0 22-23 37.89131618250315 40.0 38.0 41.0 32.0 41.0 24-25 37.75472805737805 40.0 38.0 41.0 31.0 41.0 26-27 37.61661651229676 40.0 37.0 41.0 31.0 41.0 28-29 37.515851148608235 40.0 37.0 41.0 31.0 41.0 30-31 37.288185099128285 40.0 37.0 41.0 30.0 41.0 32-33 37.070580396910884 40.0 36.5 41.0 30.0 41.0 34-35 37.068172763616076 40.0 36.0 41.0 30.0 41.0 36-37 36.91540615905349 40.0 36.0 41.0 30.0 41.0 38-39 36.64284859220572 40.0 35.0 41.0 29.5 41.0 40-41 36.65228568685536 40.0 35.0 41.0 30.0 41.0 42-43 36.57158934900246 39.5 35.0 41.0 30.0 41.0 44-45 36.50113415333952 39.0 35.0 41.0 29.0 41.0 46-47 36.36419480181068 39.0 35.0 41.0 28.5 41.0 48-49 36.15148065651318 39.0 34.5 41.0 27.5 41.0 50-51 35.68628997580265 38.5 34.0 40.5 27.0 41.0 52-53 35.66888796586879 38.5 34.0 40.0 27.5 41.0 54-55 35.87821157966436 39.0 34.5 40.5 27.0 41.0 56-57 35.82864959518007 39.0 34.5 41.0 27.0 41.0 58-59 35.49607455450074 38.5 34.0 41.0 26.0 41.0 60-61 35.24861666784924 38.0 34.0 40.0 25.5 41.0 62-63 34.85575365158101 37.5 33.5 40.0 25.0 41.0 64-65 34.40927817751691 37.0 33.0 40.0 24.0 41.0 66-67 34.11903033146206 36.5 33.0 40.0 24.0 41.0 68-69 33.7649779677022 36.0 32.0 39.0 23.5 41.0 70-71 33.478259902682574 35.0 32.0 39.0 23.5 41.0 72-73 33.214842759218904 35.0 32.0 38.5 23.5 40.5 74-75 32.93598934442376 35.0 32.0 37.0 23.0 40.0 76-77 32.66633641954955 35.0 32.0 37.0 23.0 39.0 78-79 32.32278598734221 35.0 32.0 36.5 22.0 39.0 80-81 31.867306892057947 35.0 31.0 36.0 20.0 38.5 82-83 31.241078519817158 35.0 30.5 35.5 18.0 37.0 84-85 30.553185632731882 35.0 29.5 35.0 13.0 37.0 86-87 29.899852568159524 35.0 29.0 35.0 10.0 36.5 88-89 29.393585317819042 34.0 28.5 35.0 8.0 36.0 90-91 29.088415032366747 34.0 27.5 35.0 8.0 36.0 92-93 28.83428309248971 34.0 27.0 35.0 7.0 35.5 94-95 28.62296670340547 34.0 26.5 35.0 7.0 35.0 96-97 28.447502887414036 34.0 26.0 35.0 7.0 35.0 98-99 28.29464946868768 34.0 25.0 35.0 7.0 35.0 100 27.138374415671304 33.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32303282034985514 1101 2 0.2865952623560588 1101 3 0.27068104250336944 1101 4 0.19980229943143968 1101 5 0.20359825128163322 1101 6 0.17598550238172095 1101 7 0.17636681752546224 1101 8 0.17359726996198077 1101 9 0.21275974777633166 1101 10-11 0.22202808803247365 1101 12-13 0.20422501636338808 1101 14-15 0.29658887989125304 1101 16-17 0.31948352572070604 1101 18-19 0.2880331072713247 1101 20-21 0.37096910846984343 1101 22-23 0.40669765664363666 1101 24-25 0.3372854071693965 1101 26-27 0.40283406742351957 1101 28-29 0.4148296297085068 1101 30-31 0.42324591179788484 1101 32-33 0.41071652463441666 1101 34-35 0.38645448779287506 1101 36-37 0.3058754724188404 1101 38-39 0.35773501137290964 1101 40-41 0.43296398577187745 1101 42-43 0.4097294253417445 1101 44-45 0.42989771301884616 1101 46-47 0.3953534762357833 1101 48-49 0.39814536660792044 1101 50-51 0.5778561502004749 1101 52-53 0.5901178744912201 1101 54-55 0.49861798966187365 1101 56-57 0.5867627454312583 1101 58-59 0.5864037182067818 1101 60-61 0.6458208632269233 1101 62-63 0.5993338971047137 1101 64-65 0.579894005945846 1101 66-67 0.5817422680611699 1101 68-69 0.5702052663413966 1101 70-71 0.48068629189025813 1101 72-73 0.5068652989015234 1101 74-75 0.48143540558806563 1101 76-77 0.5018346284203901 1101 78-79 0.5051612705468997 1101 80-81 0.4851388602087745 1101 82-83 0.5068428427579192 1101 84-85 0.5149518562056947 1101 86-87 0.29386096957266616 1101 88-89 0.257246466196424 1101 90-91 0.20894999549056692 1101 92-93 0.20519400659817677 1101 94-95 0.1498557131639764 1101 96-97 0.17831439872021804 1101 98-99 0.22735947325569228 1101 100 -0.06217574091705913 1102 1 0.23015558209828768 1102 2 0.21429155372315023 1102 3 0.2021685783853684 1102 4 0.1427150828404109 1102 5 0.12891654351007276 1102 6 0.13798872214299251 1102 7 0.1281820482339313 1102 8 0.08109259958509085 1102 9 0.1326457977307669 1102 10-11 0.15257964290928783 1102 12-13 0.10266638912512605 1102 14-15 0.1794567645925298 1102 16-17 0.19947564535330997 1102 18-19 0.222767076008914 1102 20-21 0.3083902361324107 1102 22-23 0.37804853531257265 1102 24-25 0.3119750524328495 1102 26-27 0.341550474373598 1102 28-29 0.3723172632561216 1102 30-31 0.3821355059732454 1102 32-33 0.31161300076581 1102 34-35 0.20778849467980365 1102 36-37 0.25591530268957996 1102 38-39 0.29361114113211784 1102 40-41 0.3496987933887894 1102 42-43 0.3490025186685628 1102 44-45 0.33909978343687897 1102 46-47 0.25032378081147755 1102 48-49 0.3162807477331384 1102 50-51 0.5323849527153897 1102 52-53 0.4798216716192414 1102 54-55 0.4215052763972764 1102 56-57 0.44728874646568073 1102 58-59 0.49538173725834866 1102 60-61 0.568802985391109 1102 62-63 0.5017246044511836 1102 64-65 0.48034808403923535 1102 66-67 0.46644303722488445 1102 68-69 0.5026426876607317 1102 70-71 0.4764837287900008 1102 72-73 0.45365327137227496 1102 74-75 0.4380383438806419 1102 76-77 0.44790932069442135 1102 78-79 0.5208304659714642 1102 80-81 0.4925745300682216 1102 82-83 0.5454888912870359 1102 84-85 0.4809335034986262 1102 86-87 0.3788182582260369 1102 88-89 0.3696762094550827 1102 90-91 0.26646170386999657 1102 92-93 0.2903904708732483 1102 94-95 0.27508342150688847 1102 96-97 0.28858367962014597 1102 98-99 0.2890882020210519 1102 100 -0.12308888333292956 1103 1 0.19978421877220498 1103 2 0.16798168809150837 1103 3 0.16086283542328772 1103 4 0.1253999518140887 1103 5 0.11779147337018969 1103 6 0.07365564262270397 1103 7 0.1130198133061171 1103 8 0.06397431756761307 1103 9 0.12139799097879944 1103 10-11 0.13430923786397386 1103 12-13 0.09784771400892822 1103 14-15 0.19345256981998205 1103 16-17 0.24667074695791058 1103 18-19 0.2098862393823353 1103 20-21 0.27380347119026993 1103 22-23 0.2951487202087648 1103 24-25 0.29527598864550697 1103 26-27 0.3305052464530789 1103 28-29 0.30717788403710955 1103 30-31 0.3072982111276801 1103 32-33 0.2717505901372803 1103 34-35 0.23693132542052098 1103 36-37 0.24993982160471973 1103 38-39 0.33378960862658147 1103 40-41 0.37160714251592 1103 42-43 0.3608345495160066 1103 44-45 0.27400758383245716 1103 46-47 0.2493645712430279 1103 48-49 0.282489150095131 1103 50-51 0.4725437802592154 1103 52-53 0.5003369761078389 1103 54-55 0.4442492261682389 1103 56-57 0.4744966983759582 1103 58-59 0.5187659008821655 1103 60-61 0.5442915537735971 1103 62-63 0.4852302442222154 1103 64-65 0.49720298053044587 1103 66-67 0.5209809500186608 1103 68-69 0.5372527594691405 1103 70-71 0.5074416240493562 1103 72-73 0.4523986153228492 1103 74-75 0.4437540867091556 1103 76-77 0.4723986930554176 1103 78-79 0.5261211491723756 1103 80-81 0.5609919657357629 1103 82-83 0.6371851153341446 1103 84-85 0.633064611163217 1103 86-87 0.5201175507734384 1103 88-89 0.4538424253299702 1103 90-91 0.44944093101604565 1103 92-93 0.4333306515817448 1103 94-95 0.42827333558110325 1103 96-97 0.39397797165915804 1103 98-99 0.4708455553050648 1103 100 0.10174777692397186 1104 1 0.09415156860302787 1104 2 0.10727755084332102 1104 3 0.10820330587919358 1104 4 0.08401916596915981 1104 5 0.03365044958778185 1104 6 0.0334024364339669 1104 7 0.03147689678738175 1104 8 0.018932264044309477 1104 9 0.08595709987821465 1104 10-11 0.08419852917481307 1104 12-13 0.06707224584803129 1104 14-15 0.13080800884468147 1104 16-17 0.16532508748150576 1104 18-19 0.10118667978820639 1104 20-21 0.19194497579445624 1104 22-23 0.27428944353172113 1104 24-25 0.2517159459399352 1104 26-27 0.26453053697133555 1104 28-29 0.3195744602802293 1104 30-31 0.3077668054317044 1104 32-33 0.21698779025557968 1104 34-35 0.1520687120559785 1104 36-37 0.21415596656382974 1104 38-39 0.29849643491414923 1104 40-41 0.29836572514224713 1104 42-43 0.3599353833107415 1104 44-45 0.3093512065100299 1104 46-47 0.2420671438037587 1104 48-49 0.25235170413526475 1104 50-51 0.4165303316952702 1104 52-53 0.4647407754139792 1104 54-55 0.4057086646586967 1104 56-57 0.47629904196713113 1104 58-59 0.5137711944130885 1104 60-61 0.5015454062879883 1104 62-63 0.5163361301013083 1104 64-65 0.4600421197169098 1104 66-67 0.46674154551393343 1104 68-69 0.4423681434196496 1104 70-71 0.4048130021040848 1104 72-73 0.450563287932944 1104 74-75 0.4173697132481564 1104 76-77 0.4930556740860652 1104 78-79 0.5261014403855526 1104 80-81 0.5788585509190831 1104 82-83 0.6105567623386179 1104 84-85 0.538147602908694 1104 86-87 0.3727830464910049 1104 88-89 0.23952753167416674 1104 90-91 0.23831631083441351 1104 92-93 0.2670145596283966 1104 94-95 0.2731579578198904 1104 96-97 0.24546023046140775 1104 98-99 0.2867441936767072 1104 100 -0.017444173008126285 1105 1 0.13678953669063887 1105 2 0.10180032471205891 1105 3 0.12662801721440786 1105 4 0.07519969334866516 1105 5 0.07170051809890055 1105 6 0.0903924569674075 1105 7 0.09409687453448612 1105 8 0.09177863530061359 1105 9 0.09825566845340461 1105 10-11 0.11729015371036411 1105 12-13 0.0855522498864687 1105 14-15 0.18965593359587274 1105 16-17 0.2106503143405618 1105 18-19 0.25995689999912486 1105 20-21 0.2743003102118209 1105 22-23 0.3650426638295059 1105 24-25 0.32497754189093797 1105 26-27 0.3401004575536106 1105 28-29 0.37150044368256374 1105 30-31 0.34985170350858397 1105 32-33 0.32184197567534767 1105 34-35 0.23940186554163034 1105 36-37 0.25340715084512766 1105 38-39 0.3075228664800491 1105 40-41 0.3500902477741761 1105 42-43 0.40459029775151834 1105 44-45 0.3162456559841047 1105 46-47 0.29505845235193107 1105 48-49 0.3052566466428317 1105 50-51 0.5376031124823726 1105 52-53 0.47563211314697895 1105 54-55 0.37398673259804127 1105 56-57 0.47868595180653273 1105 58-59 0.4994105372584201 1105 60-61 0.6033343126590083 1105 62-63 0.5690736817262092 1105 64-65 0.5627856678420216 1105 66-67 0.49536556842204504 1105 68-69 0.516105266016929 1105 70-71 0.504367693723502 1105 72-73 0.5214145955525353 1105 74-75 0.5389326314891534 1105 76-77 0.5848203586116227 1105 78-79 0.5883300138636471 1105 80-81 0.65444462307779 1105 82-83 0.6416013060427481 1105 84-85 0.6081056615620781 1105 86-87 0.47725522423179356 1105 88-89 0.3767524907945976 1105 90-91 0.4180207178287354 1105 92-93 0.4499752940042825 1105 94-95 0.4232614634886609 1105 96-97 0.4409357349710028 1105 98-99 0.45201070710583124 1105 100 0.014187570585484366 1106 1 0.016903633395870088 1106 2 2.143966941403619E-4 1106 3 0.004280024896843315 1106 4 -0.02100549911266114 1106 5 -0.04995690791704277 1106 6 -0.020945752463703116 1106 7 -0.03562160370812251 1106 8 -0.05257337038659671 1106 9 -0.02701783836691618 1106 10-11 0.009676081705215722 1106 12-13 -0.01880505738070326 1106 14-15 0.042067756505531406 1106 16-17 0.052122141232224806 1106 18-19 0.06666859911619127 1106 20-21 0.10603965324032316 1106 22-23 0.21624374627369747 1106 24-25 0.15095196117658105 1106 26-27 0.16069763389379688 1106 28-29 0.19268268620955809 1106 30-31 0.24098184654186383 1106 32-33 0.12333610683921137 1106 34-35 0.06254833085193212 1106 36-37 0.11546607605815495 1106 38-39 0.18459709690326775 1106 40-41 0.2539404844163613 1106 42-43 0.2458958267606235 1106 44-45 0.21518142353988878 1106 46-47 0.17159957422475713 1106 48-49 0.21327123580046248 1106 50-51 0.40245763648252364 1106 52-53 0.3599339303773448 1106 54-55 0.31296866605298135 1106 56-57 0.3552964309393545 1106 58-59 0.43153722566295016 1106 60-61 0.43427983438380835 1106 62-63 0.45986071175617127 1106 64-65 0.4697644950066717 1106 66-67 0.42690534245836886 1106 68-69 0.4046506096243192 1106 70-71 0.4579873296100416 1106 72-73 0.4038513507464856 1106 74-75 0.44585707618032444 1106 76-77 0.42742573676376594 1106 78-79 0.48472595825204934 1106 80-81 0.5126775148479332 1106 82-83 0.5509144189964843 1106 84-85 0.5684155920685363 1106 86-87 0.4659016517327004 1106 88-89 0.3662045764178181 1106 90-91 0.3765326196364249 1106 92-93 0.35855448864467476 1106 94-95 0.3450235092584357 1106 96-97 0.3724783202036903 1106 98-99 0.3612579073560873 1106 100 -0.01106017125976777 1107 1 0.036887509579024425 1107 2 0.03813740420952172 1107 3 0.001038919759245971 1107 4 -0.00267344647211587 1107 5 -0.0027799037198334986 1107 6 0.009770457462217053 1107 7 0.004654416951822782 1107 8 -0.025605341185517716 1107 9 0.010898488666619244 1107 10-11 0.003964267435620172 1107 12-13 -0.0407975264309286 1107 14-15 0.028948684402948288 1107 16-17 0.020679207977792657 1107 18-19 0.08194782723160898 1107 20-21 0.133947034197476 1107 22-23 0.175849150495047 1107 24-25 0.16014003903940477 1107 26-27 0.14133775088785683 1107 28-29 0.161854194737451 1107 30-31 0.1689929901716809 1107 32-33 0.12547053910494554 1107 34-35 0.06660378855834637 1107 36-37 0.061816151988537626 1107 38-39 0.18350680243000994 1107 40-41 0.2283682981698405 1107 42-43 0.21544145371904477 1107 44-45 0.15899991507701827 1107 46-47 0.14899181860200628 1107 48-49 0.21027611800099777 1107 50-51 0.36200623600353055 1107 52-53 0.31730290708045317 1107 54-55 0.29822869110272876 1107 56-57 0.3481788651732316 1107 58-59 0.34620499508989866 1107 60-61 0.39289986066433613 1107 62-63 0.4172639448163409 1107 64-65 0.44812416610535166 1107 66-67 0.39029608214750766 1107 68-69 0.4200728774331637 1107 70-71 0.3555965527409768 1107 72-73 0.38430972566684574 1107 74-75 0.4141791561755852 1107 76-77 0.4569295216527962 1107 78-79 0.4624074665775666 1107 80-81 0.47064936726267703 1107 82-83 0.5078481387263238 1107 84-85 0.5347529141852547 1107 86-87 0.41771360766148646 1107 88-89 0.29838276790011165 1107 90-91 0.24971786834494836 1107 92-93 0.24827770515519987 1107 94-95 0.2451346163401915 1107 96-97 0.2221146918238297 1107 98-99 0.25863049994440246 1107 100 -0.012129185719739866 1108 1 0.0026179967004082982 1108 2 -0.039739485782565254 1108 3 -0.02219253996118553 1108 4 -0.10299936335230342 1108 5 -0.10094170779768774 1108 6 -0.11062332225134952 1108 7 -0.07242793475253251 1108 8 -0.09265233154358299 1108 9 -0.06624849393303123 1108 10-11 -0.05733095781922515 1108 12-13 -0.10653123395307063 1108 14-15 -0.07388191107187936 1108 16-17 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2316 62-63 -1.2059988919960887 2316 64-65 -0.995163509462607 2316 66-67 -1.0341357205842883 2316 68-69 -0.8919442304667271 2316 70-71 -0.8164667018887641 2316 72-73 -0.7448608158648824 2316 74-75 -0.7873007168334247 2316 76-77 -1.0257809848747854 2316 78-79 -0.8880801186094871 2316 80-81 -1.0362826454383942 2316 82-83 -1.0347339342807729 2316 84-85 -0.9351318302190847 2316 86-87 -0.8012415480948647 2316 88-89 -0.6319780293152455 2316 90-91 -0.6681658892222799 2316 92-93 -0.5515242457761431 2316 94-95 -0.4747985089703768 2316 96-97 -0.5450755765576183 2316 98-99 -0.42357638547763443 2316 100 0.2952704856971202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10876.0 3 743.0 4 662.0 5 737.0 6 1168.0 7 949.0 8 2050.0 9 6591.0 10 15428.0 11 20625.0 12 20513.0 13 25096.0 14 34162.0 15 47556.0 16 65917.0 17 89147.0 18 119288.0 19 155931.0 20 194571.0 21 230944.0 22 270835.0 23 326985.0 24 407001.0 25 501344.0 26 556911.0 27 530929.0 28 550696.0 29 655160.0 30 798835.0 31 905411.0 32 888711.0 33 800053.0 34 928486.0 35 1370648.0 36 2418439.0 37 4812411.0 38 6192274.0 39 1981838.0 40 6306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.814206491018126 49.10625602078237 13.69785918095181 19.381678307247697 2 19.446933994461833 28.805239922605757 37.16924614043047 14.578579942501936 3 21.183691529455718 38.50978520264307 21.433683778296167 18.872839489605038 4 17.86022004267339 24.79007656253543 35.74284029931529 21.60686309547589 5 18.904019904517117 41.36586581387154 22.27745641915047 17.45265786246087 6 21.546484767960226 23.748801139923515 36.41721996494964 18.287494127166624 7 18.822206125592402 37.8704043066818 25.03292729081188 18.274462276913916 8 20.498483794780725 23.46514008144523 37.35737329495355 18.6790028288205 9 20.722550343163004 36.50411560786983 24.186233526203594 18.587100522763574 10-11 18.202992709589846 32.17860325001546 31.406562248263846 18.211841792130848 12-13 18.577157345432653 30.726543224990742 30.794073627360273 19.902225802216336 14-15 18.921851555027914 33.3035139877228 29.079339110968107 18.69529534628118 16-17 21.285289544001053 29.335050889682567 28.745365497554076 20.634294068762305 18-19 21.133464150579027 30.515736062180075 27.414147639677918 20.936652147562977 20-21 23.700608687098878 30.021766909357456 26.27035391913607 20.007270484407595 22-23 24.82601852526774 27.364781242250448 27.336048835064798 20.473151397417013 24-25 24.483946337326234 29.705975325113897 24.329414710233095 21.48066362732677 26-27 23.32710188822525 29.765849810313576 26.80008496285671 20.106963338604462 28-29 25.965876645140074 26.959621986136245 26.076086466483073 20.998414902240604 30-31 26.19978579561357 25.35011649499116 26.9808921063898 21.469205603005477 32-33 25.867188620441944 25.374193922998167 27.598926819039203 21.159690637520686 34-35 26.221085243817477 25.31930174602448 27.126949305911634 21.332663704246407 36-37 25.420575963057797 26.375615980195295 28.023823795693463 20.179984261053452 38-39 25.565835949200665 27.447748510482 27.1789377447681 19.807477795549232 40-41 27.343173191215392 27.361930941780237 25.797032769541474 19.497863097462897 42-43 25.55530366687145 29.095176165001984 25.923997479073368 19.425522689053203 44-45 24.955089137316104 29.60811755285485 26.223872835408656 19.212920474420393 46-47 25.734653786722518 28.154141327939602 26.937400326583717 19.173804558754163 48-49 25.511872135146916 26.624175745173723 28.293822930611235 19.570129189068126 50-51 25.04924404715495 26.85157588375518 28.14047870993469 19.958701359155178 52-53 24.4508241453767 27.675649444398985 27.59497794869195 20.278548461532363 54-55 23.666929059542355 26.54092945087531 28.547951459130793 21.244190030451534 56-57 24.480825081766756 25.105659168941756 27.8162608212768 22.59725492801469 58-59 25.743588463848894 24.959054313206988 26.907767578112985 22.389589644831133 60-61 26.63078641020534 24.589741496571875 26.782621614971404 21.99685047825139 62-63 26.15679071060256 24.531402264336137 26.506381984682793 22.80542504037851 64-65 24.41637640623487 24.73568875470498 26.921190370763448 23.926744468296697 66-67 24.88611111614674 24.96032706780707 26.01116269009075 24.142399125955443 68-69 23.673083120614745 26.094305791258677 27.1718832741775 23.060727813949082 70-71 21.952040480672892 27.361311513197585 28.413740041910607 22.27290796421892 72-73 21.359482500039682 28.214434553401365 28.183783434969182 22.24229951158977 74-75 20.921151885879937 30.519093142053134 27.962779053429166 20.596975918637764 76-77 20.582568516525964 32.53713898932373 26.774282189064063 20.106010305086247 78-79 20.40017359860524 34.71622446678438 25.045627685224446 19.837974249385933 80-81 20.405178712635827 35.490375543221234 24.52630637795257 19.578139366190367 82-83 20.601594951861806 35.825295578633956 24.109641951585555 19.46346751791868 84-85 21.006567168001904 35.55032293883073 23.887583241981766 19.5555266511856 86-87 21.588972654492437 34.73833547378795 23.95830538791088 19.71438648380873 88-89 22.387658013615855 33.59034168473538 24.056433392904463 19.965566908744304 90-91 23.12763786018136 32.56185428081651 24.047338361394324 20.26316949760781 92-93 23.5991315795402 31.81917861885319 24.23693619866927 20.34475360293734 94-95 24.063965025229216 31.335521558264173 24.189056508008175 20.411456908498433 96-97 24.373814498870487 30.63490764167215 24.338434869058336 20.65284299039903 98-99 24.71760630989492 30.293049069523807 24.37626885001871 20.61307577056256 100 25.316825541872873 30.239300945235048 23.860220988315692 20.583652524576383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12722.0 1 8074.5 2 3262.5 3 3084.5 4 3096.0 5 3130.5 6 3165.5 7 3259.0 8 3319.0 9 3378.0 10 3570.5 11 4331.0 12 18556.5 13 24239.5 14 17463.5 15 20095.0 16 23428.5 17 27527.0 18 31959.5 19 36998.5 20 44099.5 21 54776.0 22 69550.5 23 89242.5 24 110765.0 25 134923.5 26 165455.5 27 201544.0 28 241602.0 29 285368.0 30 363813.5 31 476099.5 32 602990.0 33 739744.5 34 865412.5 35 963174.5 36 1015659.0 37 1007670.0 38 966854.0 39 913755.0 40 865949.0 41 827161.5 42 798842.0 43 777057.0 44 759070.0 45 762643.5 46 767811.0 47 772312.5 48 771957.5 49 769072.5 50 765880.0 51 762566.0 52 760797.5 53 743208.5 54 714298.5 55 685773.5 56 651560.0 57 608284.5 58 553814.0 59 483068.0 60 398984.5 61 321479.5 62 256559.0 63 198068.5 64 149276.0 65 111586.0 66 82590.5 67 61994.5 68 47258.5 69 35895.5 70 27374.5 71 21174.5 72 16996.5 73 13446.0 74 10800.0 75 9220.0 76 7087.5 77 4531.0 78 2855.5 79 1996.0 80 1361.0 81 943.0 82 675.5 83 501.5 84 358.5 85 260.0 86 223.0 87 183.5 88 138.5 89 100.5 90 72.0 91 58.0 92 49.0 93 44.5 94 33.5 95 21.0 96 15.0 97 10.0 98 14.0 99 17.0 100 27.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07863185657012868 2 0.060182160589283364 3 0.056358868670963215 4 0.05179558476845207 5 0.05249703550346646 6 0.051101842283273025 7 0.04845405846483961 8 0.04525898890809828 9 0.04307370007978424 10-11 0.04368650594169241 12-13 0.04618012476341936 14-15 0.04301974233093698 16-17 0.04811104134716774 18-19 0.05246042131674867 20-21 0.0644814369349347 22-23 0.06674188119914314 24-25 0.06391488057203847 26-27 0.06648172776720099 28-29 0.06396305713350924 30-31 0.06469534086786491 32-33 0.0703782480589567 34-35 0.07107199054413577 36-37 0.0750012708976916 38-39 0.07276587844544796 40-41 0.07044569524501579 42-43 0.06808697079540697 44-45 0.07229567520549328 46-47 0.06980783757114281 48-49 0.07096985623381774 50-51 0.07315707212459059 52-53 0.07266374413512994 54-55 0.07498585439802095 56-57 0.07548110944994045 58-59 0.07516892533160988 60-61 0.07489913658737357 62-63 0.0746871597169022 64-65 0.07242864251515259 66-67 0.07654292086475618 68-69 0.07464476434280792 70-71 0.07928320368121346 72-73 0.07932752611776656 74-75 0.08730363763486691 76-77 0.08643453246593426 78-79 0.08056470021633588 80-81 0.07922539180744854 82-83 0.08622640972038054 84-85 0.08347263746671145 86-87 0.08262665704728475 88-89 0.08493335081050513 90-91 0.08677947664606496 92-93 0.08625146153234534 94-95 0.09249321683649804 96-97 0.08909965984649713 98-99 0.09111151305351642 100 0.09126567805022287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5946227E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.70241010323016 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4609719148423 32.54123931307541 2 13.637201511440896 11.919571462267601 3 4.661849184459949 6.112021346960331 4 2.1649254457668237 3.7844983869528033 5 1.219675404016934 2.6651377349585488 6 0.7752459658641507 2.0328070278641928 7 0.5554129424341591 1.6991018930829567 8 0.4076205076978807 1.4251198875119735 9 0.30924792071156615 1.216339150905726 >10 1.5750226609269433 12.263945715426857 >50 0.15295087133801177 4.712878086824335 >100 0.07730178620408815 5.026008583811521 >500 0.0012649459947303627 0.3874477616639277 >1k 8.998133912511818E-4 0.8351509598385853 >5k 1.9566843539570291E-4 0.6013813232765098 >10k+ 2.1345647497644272E-4 12.777351365578662 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG 789300 3.0420607975101737 Illumina Single End PCR Primer 1 (100% over 32bp) ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA 616631 2.376572902102491 Illumina Single End PCR Primer 1 (100% over 34bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 377258 1.453999458187119 No Hit ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG 266229 1.0260798227040873 Illumina Single End PCR Primer 1 (100% over 30bp) ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA 246808 0.9512288626781844 Illumina Single End PCR Primer 1 (100% over 36bp) ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC 213747 0.8238076387753794 Illumina Single End PCR Primer 1 (100% over 38bp) ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG 171014 0.6591093186689533 Illumina Single End PCR Primer 1 (100% over 28bp) ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA 107725 0.4151856067550785 Illumina Single End PCR Primer 1 (100% over 26bp) ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC 106245 0.40948150187693955 Illumina Single End PCR Primer 1 (100% over 40bp) ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG 72508 0.2794548895297956 Illumina Single End PCR Primer 1 (100% over 24bp) ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 44840 0.17281896130793892 Illumina Single End PCR Primer 1 (100% over 37bp) TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 37797 0.1456743595128494 Illumina Single End PCR Primer 1 (100% over 37bp) ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG 37398 0.1441365636707025 Illumina Single End PCR Primer 1 (100% over 22bp) ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT 30707 0.1183486138466298 Illumina Single End PCR Primer 1 (100% over 39bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.8114387113008762E-4 0.0 0.0 0.0 3.8541249176614384E-6 2 1.9270624588307194E-4 3.8541249176614384E-6 0.0 0.0 3.8541249176614384E-6 3 2.4666399473033206E-4 1.9270624588307195E-5 0.0 3.8541249176614384E-6 3.8541249176614384E-6 4 2.5437224456565494E-4 3.854124917661439E-5 0.0 3.8541249176614384E-6 7.708249835322877E-6 5 4.779114897900184E-4 3.854124917661439E-5 0.0 3.8541249176614384E-6 1.5416499670645754E-5 6 5.048903642136485E-4 4.6249499011937265E-5 0.0 3.8541249176614384E-6 1.5416499670645754E-5 7 7.862414832029335E-4 5.0103623929598706E-5 0.0 3.8541249176614384E-6 1.5416499670645754E-5 8 8.864487310621309E-4 5.781187376492158E-5 0.0 3.8541249176614384E-6 1.9270624588307195E-5 9 0.054481909836062097 8.093662327089022E-5 0.0 3.8541249176614384E-6 1.9270624588307195E-5 10-11 0.3028205218431181 9.635312294153597E-5 0.0 9.635312294153596E-6 1.9270624588307195E-5 12-13 1.1943297189221385 1.0406137277685885E-4 0.0 1.3489437211815035E-5 2.5051811964799353E-5 14-15 2.4933760118571384 1.0791549769452028E-4 0.0 1.5416499670645754E-5 2.697887442363007E-5 16-17 4.496792539431649 1.2525905982399674E-4 0.0 1.9270624588307195E-5 3.083299934129151E-5 18-19 7.647302630937439 1.4452968441230397E-4 0.0 2.3124749505968632E-5 3.854124917661438E-5 20-21 10.037588509496969 1.7150855883593403E-4 0.0 2.3124749505968632E-5 4.8176561470767985E-5 22-23 11.098218635025432 1.869250585065798E-4 0.0 3.083299934129151E-5 5.781187376492158E-5 24-25 11.828563744547521 2.081227455537177E-4 0.0 3.083299934129151E-5 5.781187376492158E-5 26-27 12.326559079283474 2.177580578478713E-4 0.0 3.468712425895295E-5 5.781187376492158E-5 28-29 12.641972954295051 2.2739337014202488E-4 0.0 3.661418671778367E-5 6.166599868258302E-5 30-31 12.830428100393942 2.408828073538399E-4 0.0 3.854124917661439E-5 6.552012360024446E-5 32-33 12.944652800578673 2.6593461931863924E-4 0.0 4.04683116354451E-5 7.130131097673662E-5 34-35 13.008274767656971 2.7749699407162364E-4 0.0 4.2395374094275824E-5 7.322837343556734E-5 36-37 13.041751696691776 2.8520524390694647E-4 0.0 4.2395374094275824E-5 7.708249835322878E-5 38-39 13.063479325915093 2.986946811187615E-4 0.0 4.8176561470767985E-5 8.093662327089022E-5 40-41 13.083744314732158 3.0062174357759223E-4 0.0 5.781187376492158E-5 8.671781064738237E-5 42-43 13.10587277294691 3.0254880603642297E-4 0.0 5.781187376492158E-5 9.249899802387453E-5 44-45 13.132290872195021 3.237464930835608E-4 0.0 5.9738936223752295E-5 9.442606048270525E-5 46-47 13.16334355665662 3.2952768046005303E-4 0.0 6.552012360024446E-5 9.828018540036669E-5 48-49 13.20016201199504 3.507253675071909E-4 0.0 6.93742485179059E-5 1.0791549769452028E-4 50-51 13.259760272659296 3.584336173425138E-4 0.0 6.93742485179059E-5 1.1176962261218172E-4 52-53 13.327928180077974 3.699959920954981E-4 1.9270624588307192E-6 6.93742485179059E-5 1.1755080998867388E-4 54-55 13.390721510298974 3.815583668484824E-4 3.8541249176614384E-6 6.93742485179059E-5 1.194778724475046E-4 56-57 13.465626428073723 3.969748665191282E-4 3.8541249176614384E-6 8.09366232708902E-5 1.2333199736516603E-4 58-59 13.552750848899919 4.200996160250968E-4 3.8541249176614384E-6 8.671781064738237E-5 1.310402472004889E-4 60-61 13.655257082272502 4.2973492831925044E-4 3.8541249176614384E-6 9.057193556504381E-5 1.3296730965931962E-4 62-63 13.774590425035594 4.509326153663883E-4 3.8541249176614384E-6 9.635312294153597E-5 1.387484970358118E-4 64-65 13.911629617670421 4.644220525782034E-4 3.8541249176614384E-6 9.635312294153597E-5 1.4260262195347323E-4 66-67 14.069721967667977 4.798385522488491E-4 3.8541249176614384E-6 9.828018540036669E-5 1.4452968441230397E-4 68-69 14.25128593841409 4.87546802084172E-4 3.8541249176614384E-6 1.0406137277685885E-4 1.6572737145944188E-4 70-71 14.456369706470232 5.183798014254636E-4 3.8541249176614384E-6 1.1176962261218172E-4 1.695814963771033E-4 72-73 14.689002759437816 5.434316133902629E-4 3.8541249176614384E-6 1.1947787244750459E-4 1.695814963771033E-4 74-75 14.95056294697491 5.569210506020779E-4 3.8541249176614384E-6 1.2525905982399674E-4 1.7343562129476474E-4 76-77 15.24436674357316 5.684834253550622E-4 3.8541249176614384E-6 1.291131847416582E-4 1.869250585065798E-4 78-79 15.56834641121424 5.819728625668773E-4 5.781187376492158E-6 1.310402472004889E-4 1.9270624588307194E-4 80-81 15.92586274682635 5.896811124022002E-4 7.708249835322877E-6 1.310402472004889E-4 1.9656037080073338E-4 82-83 16.316449401294456 5.954622997786923E-4 7.708249835322877E-6 1.3296730965931962E-4 2.0234155817722556E-4 84-85 16.740896470226673 6.108787994493381E-4 7.708249835322877E-6 1.3489437211815035E-4 2.081227455537177E-4 86-87 17.202994485479525 6.224411742023223E-4 7.708249835322877E-6 1.387484970358118E-4 2.1197687047137915E-4 88 17.569490932149787 6.282223615788145E-4 7.708249835322877E-6 1.4645674687113468E-4 2.1583099538904056E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 92675 0.0 81.74634 8 GTGGTAT 93235 0.0 81.30824 6 TGGTATC 93325 0.0 81.01179 7 AGTGGTA 94145 0.0 80.67515 5 GTATCAA 96025 0.0 78.8437 9 GCAGTGG 103000 0.0 74.50324 3 AAGCAGT 104090 0.0 74.045586 1 CAGTGGT 102825 0.0 74.01687 4 AGCAGTG 111830 0.0 68.87529 2 CAACGCA 88995 0.0 44.270657 12-13 ATCAACG 89680 0.0 43.816097 10-11 ACGCAGA 90940 0.0 43.328156 14-15 TCAACGC 87735 0.0 43.323875 12-13 AACGCAG 90530 0.0 42.148754 14-15 AGAGTAC 89780 0.0 41.846878 18-19 CGCAGAG 92265 0.0 41.209335 16-17 TATCAAC 93965 0.0 40.300385 10-11 CGTACCG 2325 0.0 39.205936 3 GAGTACT 56000 0.0 38.698696 20-21 CAGAGTA 97235 0.0 37.92801 18-19 >>END_MODULE