FastQCFastQC Report
Thu 26 May 2016
SRR1660593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25946227
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9160113.5304208199519724No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8353903.2196974149651894Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6394772.4646242399713842Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG2951881.1376914261946447Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT2520020.971247187500518TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG2172240.8372084311140884TruSeq Adapter, Index 20 (100% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC2029210.7820828824167769Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1374830.5298766560548476Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC1081030.41664246597395455TruSeq Adapter, Index 20 (100% over 39bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA990360.38169711534551826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG541310.20862763591793132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG456510.17594465661616235TruSeq Adapter, Index 15 (97% over 36bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG385800.1486921393233783TruSeq Adapter, Index 15 (97% over 36bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC374130.1441943755444674No Hit
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC312770.12054546504969682TruSeq Adapter, Index 20 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT279500.10772279144863721TruSeq Adapter, Index 20 (97% over 44bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC263690.10162941995381447TruSeq Adapter, Index 20 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1375500.085.163998
GTGGTAT1398850.083.836476
TGGTATC1397500.083.779577
GTATCAA1398900.083.668859
AGTGGTA1404350.083.652045
AAGCAGT1482200.080.272371
GCAGTGG1485550.079.667123
CAGTGGT1484550.079.496184
AGCAGTG1574750.075.4796142
CAACGCA1352750.045.246512-13
ATCAACG1365350.044.7502510-11
ACGCAGA1378450.044.39609514-15
TCAACGC1349250.043.47776412-13
AGAGTAC1378450.043.44411518-19
AACGCAG1369900.042.71260514-15
CGCAGAG1388650.041.98697316-17
TATCAAC1409050.041.5763210-11
AGTACTT1218900.041.20488720-21
GAGTACT1155700.041.1563720-21
GCAGAGT1517350.040.136916-17
Produced by FastQC (version 0.11.5)