FastQCFastQC Report
Thu 26 May 2016
SRR1660592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26588089
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG8238163.0984400571248276Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA6188282.3274632486749987Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2956231.1118625336330112Illumina Single End PCR Primer 1 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2855831.0741012639155827No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA2101620.7904366500352845Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG2013460.757278945470658Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1768230.6650459158610459Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1392820.5238511124285766Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG1001940.3768379141502046Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC908580.34172444661216533Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG563950.21210625554924237Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT426380.16036504165455442Illumina Single End PCR Primer 1 (100% over 37bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT411090.154614346296193No Hit
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT321420.12088871825274843Illumina Single End PCR Primer 1 (100% over 37bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT290280.10917670690811965Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA998700.082.073398
GTGGTAT997000.082.055256
AGTGGTA1002000.081.8027045
TGGTATC1001450.081.594957
GTATCAA1029750.079.532139
AAGCAGT1103200.075.370361
CAGTGGT1116650.073.5555344
GCAGTGG1129100.073.303953
AGCAGTG1185350.070.103662
CAACGCA967500.043.97359512-13
ATCAACG974550.043.50768310-11
TCAACGC954350.043.15154612-13
ACGCAGA1002250.042.46239514-15
AACGCAG994400.041.6089814-15
AGAGTAC981150.041.4999818-19
TATCAAC1006700.040.75753410-11
CGCAGAG1016850.040.57956316-17
CAGAGTA1054500.038.09184318-19
GCAGAGT1113750.037.82543616-17
GTACATG594600.036.84723322-23
Produced by FastQC (version 0.11.5)