##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660592_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26588089 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82830706637096 34.0 31.0 34.0 30.0 34.0 2 31.942757337693582 34.0 31.0 34.0 30.0 34.0 3 31.897997407786622 34.0 31.0 34.0 30.0 34.0 4 35.234296605521365 37.0 35.0 37.0 33.0 37.0 5 35.21513219697738 37.0 35.0 37.0 33.0 37.0 6 35.18245718975892 37.0 35.0 37.0 32.0 37.0 7 35.072376130529726 37.0 35.0 37.0 32.0 37.0 8 35.0876498119139 37.0 35.0 37.0 32.0 37.0 9 36.72260443388767 39.0 37.0 39.0 33.0 39.0 10-11 36.70278817330572 39.0 37.0 39.0 33.0 39.0 12-13 36.68125125502627 39.0 37.0 39.0 33.0 39.0 14-15 38.118339381216906 40.0 38.0 41.0 33.0 41.0 16-17 38.078891942929786 40.0 38.0 41.0 33.0 41.0 18-19 38.05814451350753 40.0 38.0 41.0 32.0 41.0 20-21 37.979323335347644 40.0 38.0 41.0 32.0 41.0 22-23 37.88285132113106 40.0 38.0 41.0 31.0 41.0 24-25 37.753533696987404 40.0 38.0 41.0 31.0 41.0 26-27 37.64024097406925 40.0 37.5 41.0 30.5 41.0 28-29 37.53869548503467 40.0 37.0 41.0 30.0 41.0 30-31 37.30737308724971 40.0 37.0 41.0 30.0 41.0 32-33 37.045568713118115 40.0 36.5 41.0 30.0 41.0 34-35 36.97914116730992 40.0 36.0 41.0 30.0 41.0 36-37 36.81037856838827 40.0 36.0 41.0 30.0 41.0 38-39 36.55069939400308 40.0 35.0 41.0 29.5 41.0 40-41 36.564520488854996 39.5 35.0 41.0 29.5 41.0 42-43 36.47193371061756 39.5 35.0 41.0 29.5 41.0 44-45 36.359398093635086 39.0 35.0 41.0 29.0 41.0 46-47 36.18939048233214 39.0 35.0 41.0 28.5 41.0 48-49 35.96110427492551 39.0 34.5 41.0 27.5 41.0 50-51 35.617371259739656 38.5 33.5 40.5 27.0 41.0 52-53 35.534502724133354 38.0 34.0 40.0 26.5 41.0 54-55 35.655219485687745 38.5 34.0 40.0 27.0 41.0 56-57 35.64511629624829 38.5 34.0 41.0 26.5 41.0 58-59 35.321436865959036 38.0 34.0 40.0 26.0 41.0 60-61 35.120083000323945 37.5 33.5 40.0 26.0 41.0 62-63 34.728058831155565 37.0 33.0 40.0 25.5 41.0 64-65 34.25975125553401 36.5 33.0 40.0 24.5 41.0 66-67 33.92875255156548 36.0 32.5 39.0 24.0 41.0 68-69 33.54486994157421 35.0 32.0 39.0 24.0 41.0 70-71 33.24006368039463 35.0 32.0 38.5 23.5 40.5 72-73 32.96697397093864 35.0 32.0 37.0 23.5 40.0 74-75 32.7078633406109 35.0 32.0 37.0 23.5 39.0 76-77 32.47165672568646 35.0 32.0 36.0 23.0 39.0 78-79 32.18251990581196 35.0 32.0 36.0 23.0 38.5 80-81 31.802597979117643 35.0 31.0 35.5 21.0 37.0 82-83 31.27095574638704 35.0 31.0 35.0 19.5 37.0 84-85 30.654670217178825 35.0 30.0 35.0 16.0 36.5 86-87 29.996805656096605 35.0 29.0 35.0 10.5 36.0 88-89 29.473042383753118 34.0 28.5 35.0 8.0 36.0 90-91 29.170452641406456 34.0 28.0 35.0 8.0 35.5 92-93 28.927955954262075 34.0 27.0 35.0 7.5 35.0 94-95 28.720995292290468 34.0 27.0 35.0 7.0 35.0 96-97 28.540172368160796 34.0 26.5 35.0 7.0 35.0 98-99 28.383653089923087 34.0 25.5 35.0 7.0 35.0 100 26.885592116078744 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3079015231586695 1101 2 0.2866260303036725 1101 3 0.29436534534839254 1101 4 0.21443296426924263 1101 5 0.18028818991946594 1101 6 0.13178603807360645 1101 7 0.14580142221275594 1101 8 0.10336426309336133 1101 9 0.18409089768499598 1101 10-11 0.1674150730553876 1101 12-13 0.11887138778618578 1101 14-15 0.25155546076317137 1101 16-17 0.23274929193883054 1101 18-19 0.23863879749990957 1101 20-21 0.3081522186218777 1101 22-23 0.34932397150197403 1101 24-25 0.2912894855272299 1101 26-27 0.3694825696796329 1101 28-29 0.3812519651608923 1101 30-31 0.36300823988352704 1101 32-33 0.3138539374322207 1101 34-35 0.27758835605160925 1101 36-37 0.23987863929234265 1101 38-39 0.33276345891744796 1101 40-41 0.43177495613011985 1101 42-43 0.39627696435003656 1101 44-45 0.37939881654737917 1101 46-47 0.33600581171146615 1101 48-49 0.34729280155289644 1101 50-51 0.5750211253895614 1101 52-53 0.5596447531523481 1101 54-55 0.5164649398113923 1101 56-57 0.6348468082135099 1101 58-59 0.6202498390750151 1101 60-61 0.683733765574182 1101 62-63 0.6642954444922893 1101 64-65 0.6398794388751057 1101 66-67 0.6113259369153781 1101 68-69 0.6288198237372882 1101 70-71 0.5463064745650641 1101 72-73 0.5682465118548023 1101 74-75 0.5740617628247477 1101 76-77 0.5879500500183639 1101 78-79 0.6440328302624856 1101 80-81 0.6577749257416983 1101 82-83 0.6864839999048051 1101 84-85 0.6767816048670987 1101 86-87 0.471643523499619 1101 88-89 0.4153432308650693 1101 90-91 0.2972930556929114 1101 92-93 0.31512342047218667 1101 94-95 0.31677710502460243 1101 96-97 0.3493648222502834 1101 98-99 0.33001894958639255 1101 100 -0.06873376563268252 1102 1 0.19334603888130886 1102 2 0.18593408860158434 1102 3 0.1811794622243177 1102 4 0.12439759670063921 1102 5 0.08589304862436364 1102 6 0.08285412043592544 1102 7 0.0715102575867661 1102 8 0.033372788043358526 1102 9 0.06295831219988912 1102 10-11 0.10342738270069418 1102 12-13 0.01790985831753744 1102 14-15 0.0922545576822742 1102 16-17 0.14312826318683847 1102 18-19 0.1674686166957784 1102 20-21 0.2052291089651206 1102 22-23 0.3425415792988744 1102 24-25 0.3034247656479323 1102 26-27 0.2942284874995522 1102 28-29 0.34103330899976214 1102 30-31 0.3376324088342102 1102 32-33 0.25504941177462825 1102 34-35 0.1682619547796591 1102 36-37 0.21128420682275362 1102 38-39 0.23725136649271406 1102 40-41 0.3524484431002577 1102 42-43 0.3731819234765581 1102 44-45 0.3031389892763201 1102 46-47 0.2668855178552363 1102 48-49 0.2861490604392074 1102 50-51 0.544583269873165 1102 52-53 0.4879662228388284 1102 54-55 0.4488190845520492 1102 56-57 0.5127948326486376 1102 58-59 0.5382096004395365 1102 60-61 0.585921349706787 1102 62-63 0.6083186126325302 1102 64-65 0.5852836615212524 1102 66-67 0.5670586180711581 1102 68-69 0.5817762453559894 1102 70-71 0.5819746212679675 1102 72-73 0.5392104258357051 1102 74-75 0.5639941414714755 1102 76-77 0.5904522719878145 1102 78-79 0.6543384391572538 1102 80-81 0.644414683657736 1102 82-83 0.7156427843037321 1102 84-85 0.6723189329135373 1102 86-87 0.5223947646146812 1102 88-89 0.46382348371306037 1102 90-91 0.39265337541422696 1102 92-93 0.3957318187578309 1102 94-95 0.3768174792715584 1102 96-97 0.3847352411959619 1102 98-99 0.3863313322805624 1102 100 -0.07638317003889838 1103 1 0.14038123239999578 1103 2 0.10882822578607687 1103 3 0.08137375945097958 1103 4 0.0401826419273803 1103 5 0.03531759865143158 1103 6 0.008390853919209462 1103 7 0.03331994971700425 1103 8 -0.012498138640104628 1103 9 0.013261078928927361 1103 10-11 0.048915024481637204 1103 12-13 -0.008013152520788935 1103 14-15 0.09840784974975492 1103 16-17 0.14861190220844378 1103 18-19 0.138724646036529 1103 20-21 0.20707817532913708 1103 22-23 0.27752259217410113 1103 24-25 0.259444519093158 1103 26-27 0.29458380447348986 1103 28-29 0.27753692538178854 1103 30-31 0.26662859831355235 1103 32-33 0.23029283989330196 1103 34-35 0.18446806541136596 1103 36-37 0.21415170888834467 1103 38-39 0.27813244761318145 1103 40-41 0.3496722924816922 1103 42-43 0.349774338726462 1103 44-45 0.25897146724818043 1103 46-47 0.2239844428721014 1103 48-49 0.24562418717205503 1103 50-51 0.4702204548542923 1103 52-53 0.4771144492227748 1103 54-55 0.41902430070297925 1103 56-57 0.4989624035592115 1103 58-59 0.5221901432444085 1103 60-61 0.5857974962739476 1103 62-63 0.5327622163815491 1103 64-65 0.527931795147289 1103 66-67 0.49194741521556296 1103 68-69 0.5391061693034445 1103 70-71 0.5301158106233075 1103 72-73 0.48813795223345835 1103 74-75 0.47812983473892956 1103 76-77 0.5522452399441562 1103 78-79 0.5947694424275731 1103 80-81 0.6626037505753857 1103 82-83 0.7167235053288614 1103 84-85 0.7446139167796808 1103 86-87 0.5465376829995172 1103 88-89 0.4517642424237138 1103 90-91 0.4230490517586034 1103 92-93 0.4235932383050631 1103 94-95 0.4482035081655269 1103 96-97 0.4624854118776227 1103 98-99 0.4674202856264742 1103 100 0.05530284353248405 1104 1 0.05447807806453753 1104 2 0.09441256949447308 1104 3 0.04208932842591295 1104 4 -0.008629266205247177 1104 5 -0.03173965764615616 1104 6 -0.028866579466225062 1104 7 -0.03219359558817558 1104 8 -0.06346160264830303 1104 9 -0.07424088811230689 1104 10-11 -0.020793578910335953 1104 12-13 -0.05901261423964854 1104 14-15 0.01202587518812237 1104 16-17 0.06160284472507982 1104 18-19 0.059556635004511804 1104 20-21 0.13528555543886256 1104 22-23 0.25282159057022824 1104 24-25 0.23797515255691337 1104 26-27 0.27307530724623064 1104 28-29 0.2907138636800255 1104 30-31 0.2802247082820202 1104 32-33 0.18474478213322953 1104 34-35 0.11403960811662017 1104 36-37 0.16627226494812675 1104 38-39 0.3113329715201303 1104 40-41 0.30988746491453156 1104 42-43 0.32373971568074467 1104 44-45 0.2574736224653975 1104 46-47 0.20674038242691495 1104 48-49 0.19474154160437962 1104 50-51 0.43563009313253787 1104 52-53 0.45164558552685463 1104 54-55 0.3951978935212779 1104 56-57 0.5034931773957112 1104 58-59 0.5488508339982445 1104 60-61 0.5940650794503384 1104 62-63 0.5496499325244244 1104 64-65 0.5756812986628645 1104 66-67 0.5676359355099976 1104 68-69 0.558071765696063 1104 70-71 0.4894673475795841 1104 72-73 0.5447112921054824 1104 74-75 0.5362620766234301 1104 76-77 0.5713940417509917 1104 78-79 0.6035848518192317 1104 80-81 0.6513513554927641 1104 82-83 0.718585683816503 1104 84-85 0.6702874689859648 1104 86-87 0.5267501440683269 1104 88-89 0.4012201541661682 1104 90-91 0.44228456526046145 1104 92-93 0.4627318512758798 1104 94-95 0.4303710138252477 1104 96-97 0.437421791363267 1104 98-99 0.469788256680868 1104 100 0.1060912688022384 1105 1 0.026194462576217603 1105 2 -0.0034597348264142624 1105 3 0.023521559064914044 1105 4 -0.05888570319149977 1105 5 -0.09114465216448053 1105 6 -0.05485134700216321 1105 7 -0.08792106019331669 1105 8 -0.07156971782258381 1105 9 -0.10452969477161389 1105 10-11 -0.03649439240694363 1105 12-13 -0.11249430080319911 1105 14-15 -0.00397641190463105 1105 16-17 0.056239484820295615 1105 18-19 0.07002322091074831 1105 20-21 0.1493977745241608 1105 22-23 0.2385961234243794 1105 24-25 0.2144220089159461 1105 26-27 0.25618086580252 1105 28-29 0.28474391212213135 1105 30-31 0.28132873511563616 1105 32-33 0.24990662924955842 1105 34-35 0.17855440481907436 1105 36-37 0.17627462964284035 1105 38-39 0.2763088786948771 1105 40-41 0.3063522055262595 1105 42-43 0.323660387667033 1105 44-45 0.2696520366828068 1105 46-47 0.23749834618163845 1105 48-49 0.2343177067140232 1105 50-51 0.5161388613622293 1105 52-53 0.4536615835191924 1105 54-55 0.36311497300341244 1105 56-57 0.510566488843402 1105 58-59 0.49505997192797224 1105 60-61 0.5626885931585264 1105 62-63 0.5108349376622243 1105 64-65 0.5514735001162023 1105 66-67 0.4944711246984852 1105 68-69 0.4961328317720515 1105 70-71 0.4805496608538391 1105 72-73 0.5241813798414867 1105 74-75 0.5294310023367998 1105 76-77 0.5768120011243809 1105 78-79 0.6363329026738249 1105 80-81 0.7034786964676769 1105 82-83 0.665778543071287 1105 84-85 0.6766028308337972 1105 86-87 0.5255325202984125 1105 88-89 0.4492754184137233 1105 90-91 0.42746657056653703 1105 92-93 0.46589362295511094 1105 94-95 0.4928147501194893 1105 96-97 0.4924693296369753 1105 98-99 0.505101039595985 1105 100 0.047993254371437644 1106 1 9.645990737290333E-4 1106 2 0.014088552115062214 1106 3 -0.02141359594781278 1106 4 -0.07378518125903355 1106 5 -0.0696315714206861 1106 6 -0.052755791680660025 1106 7 -0.09016302087916017 1106 8 -0.09351436742075236 1106 9 -0.08214764495843951 1106 10-11 -0.04169336190019379 1106 12-13 -0.08277146784728018 1106 14-15 -0.039086400164840995 1106 16-17 -0.0070036941575182254 1106 18-19 0.016507522958534082 1106 20-21 0.07234099164986674 1106 22-23 0.18145750080717704 1106 24-25 0.1586102151174842 1106 26-27 0.12121485611655203 1106 28-29 0.15959866355716912 1106 30-31 0.17644536875077677 1106 32-33 0.07639839229209144 1106 34-35 0.013077290501286143 1106 36-37 0.03513048360964177 1106 38-39 0.13953743752674796 1106 40-41 0.1957724106128964 1106 42-43 0.19125946139264016 1106 44-45 0.1453684069754928 1106 46-47 0.12430012262731083 1106 48-49 0.13912295259450502 1106 50-51 0.3959886331813536 1106 52-53 0.3188635248870426 1106 54-55 0.2879060707698926 1106 56-57 0.4045429164671006 1106 58-59 0.47180459638320826 1106 60-61 0.48580934727933567 1106 62-63 0.4649827995822804 1106 64-65 0.44577428513422745 1106 66-67 0.4179102467062137 1106 68-69 0.3777043802551887 1106 70-71 0.3956305525189734 1106 72-73 0.42301324768153137 1106 74-75 0.44858694178312675 1106 76-77 0.5075822336338263 1106 78-79 0.5685250848614523 1106 80-81 0.556632748107333 1106 82-83 0.5843554855630266 1106 84-85 0.5887229069304851 1106 86-87 0.46773803176911244 1106 88-89 0.38992294268643946 1106 90-91 0.3414860229388381 1106 92-93 0.348093671539079 1106 94-95 0.3467665790987837 1106 96-97 0.31808934199329286 1106 98-99 0.3250270943465523 1106 100 -0.1151525286813957 1107 1 -0.02822097397292822 1107 2 -0.015238140919606735 1107 3 -0.04771430800702703 1107 4 -0.09398441607870467 1107 5 -0.09955260629078566 1107 6 -0.07774247835009618 1107 7 -0.07189398793637736 1107 8 -0.07979056468771262 1107 9 -0.08341717628734102 1107 10-11 -0.07251031906879746 1107 12-13 -0.11725733851478992 1107 14-15 -0.0476568861693778 1107 16-17 -0.043753018082824724 1107 18-19 0.00792026522699274 1107 20-21 0.06327914297111903 1107 22-23 0.11116792780350693 1107 24-25 0.08617951118208111 1107 26-27 0.14096506747175397 1107 28-29 0.1523351829400852 1107 30-31 0.15074483533128102 1107 32-33 0.08597080680002733 1107 34-35 0.02368175208073353 1107 36-37 0.046834570733267356 1107 38-39 0.14447676564078904 1107 40-41 0.20339254644407134 1107 42-43 0.1526104072435288 1107 44-45 0.13942540291265004 1107 46-47 0.09455104663727099 1107 48-49 0.14408046094997928 1107 50-51 0.35832645953652076 1107 52-53 0.3141130968455528 1107 54-55 0.2816519785802072 1107 56-57 0.3671617196492676 1107 58-59 0.3548597447472588 1107 60-61 0.40875663264768747 1107 62-63 0.45157139402999036 1107 64-65 0.508518223233871 1107 66-67 0.46831428729944236 1107 68-69 0.4406775799022782 1107 70-71 0.41240700657472473 1107 72-73 0.43823336060249574 1107 74-75 0.4964159287698635 1107 76-77 0.5301290129309706 1107 78-79 0.550541522067423 1107 80-81 0.5668889083940627 1107 82-83 0.6091801173282327 1107 84-85 0.6310565732791709 1107 86-87 0.5324146884801735 1107 88-89 0.3849944760473214 1107 90-91 0.32788165894090326 1107 92-93 0.3933114200225738 1107 94-95 0.3989331815720796 1107 96-97 0.3655341281658373 1107 98-99 0.4037001897026684 1107 100 -0.04070076335879591 1108 1 -0.014091565203447232 1108 2 -0.02891167169872233 1108 3 -0.027076144313745942 1108 4 -0.1192644494796653 1108 5 -0.1432833702927283 1108 6 -0.16428659103058862 1108 7 -0.12172619937798146 1108 8 -0.14625696179298586 1108 9 -0.15341530885707044 1108 10-11 -0.09908981199198053 1108 12-13 -0.1526738393562468 1108 14-15 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-0.8538511802718389 2316 70-71 -0.8329407610891764 2316 72-73 -0.8328460522681489 2316 74-75 -0.9514393424156395 2316 76-77 -1.1292993663980084 2316 78-79 -0.9549068517474986 2316 80-81 -1.1353876073544242 2316 82-83 -1.15385845371463 2316 84-85 -1.1008320593160619 2316 86-87 -1.0010838862795488 2316 88-89 -0.7379237855910503 2316 90-91 -0.7434028556977559 2316 92-93 -0.6805839332652361 2316 94-95 -0.5580668168880827 2316 96-97 -0.7102750419616193 2316 98-99 -0.6945661044757685 2316 100 0.17932505174210078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13825.0 3 515.0 4 363.0 5 487.0 6 703.0 7 741.0 8 2015.0 9 6910.0 10 19377.0 11 27322.0 12 24555.0 13 26872.0 14 34913.0 15 48965.0 16 68027.0 17 91960.0 18 123760.0 19 165967.0 20 211008.0 21 247543.0 22 288642.0 23 338741.0 24 413204.0 25 515739.0 26 581988.0 27 530255.0 28 539092.0 29 649754.0 30 788423.0 31 926546.0 32 977985.0 33 871560.0 34 957315.0 35 1441147.0 36 2642417.0 37 5301305.0 38 6072747.0 39 1630659.0 40 4742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.490103486688557 47.441646527473644 13.800223847577378 20.268026138260414 2 20.43707786370346 28.548267014561183 35.41786520738446 15.596789914350898 3 22.24840895022328 38.12388751463242 20.068464517286728 19.559239017857575 4 18.751050202314364 24.12862044370255 34.78294903094622 22.337380323036868 5 19.61401521028999 41.14874153123613 21.18325354456223 18.05398971391165 6 22.355398272077853 22.979357195860857 35.57905608487527 19.086188447186018 7 19.45984218662741 37.46170195791543 24.02438023930914 19.054075616148022 8 21.434935157992008 22.825420922599434 36.37715354315493 19.36249037625363 9 21.450815792150422 36.23221084589227 22.866316597505673 19.450656764451644 10-11 18.91720427491577 31.832397294485816 30.365562320137535 18.88483611046088 12-13 19.2024193980086 30.262543933484242 29.830290850273027 20.704745818234127 14-15 19.509891562730708 32.969290529059954 28.18396055789328 19.336857350316063 16-17 21.860296759795425 28.878644828658274 27.938717503059646 21.32234090848665 18-19 21.5027943158595 30.105603269073043 26.732550252140136 21.659052162927317 20-21 24.172123693588752 29.596635583281746 25.63959057742625 20.591650145703248 22-23 25.365488741475406 26.765589834075996 26.790841343836462 21.07808008061214 24-25 24.876279376027146 29.277034465493333 23.68230987014641 22.164376288333106 26-27 23.772113263218674 29.68399063168284 25.88955722731428 20.654338877784205 28-29 27.1102629957873 26.636153407736053 24.742701385928275 21.510882210548374 30-31 27.34166789796106 24.96117753242883 25.602042741860153 22.095111827749957 32-33 26.78032215777187 25.056206873360026 26.215490925549574 21.947980043318537 34-35 27.00771473983467 24.92618097363546 25.820095463135612 22.246008823394252 36-37 26.233305190615226 25.795298384573854 26.8086921705533 21.162704254257612 38-39 26.518232569982263 26.779868789517764 25.98242114889481 20.719477491605158 40-41 28.349992773674497 26.679906148097523 24.541736169866823 20.42836490836116 42-43 26.581418131134903 28.489402915626975 24.60367044828797 20.32550850495015 44-45 25.957844129789887 29.11055508758857 24.848933940766 20.08266684185554 46-47 26.78463868911868 27.584812659945595 25.520265885112714 20.110282765823012 48-49 26.597211183336345 26.044822040781256 26.90265630612327 20.455310469759134 50-51 26.27560025540741 26.416738632516495 26.682195231604577 20.625465880471513 52-53 25.64049022959572 27.31361977689461 26.22030997475851 20.825580018751165 54-55 24.643639570891345 26.2587060980286 27.29811404948898 21.799540281591074 56-57 25.260341916033173 24.88920976952956 26.696664385520215 23.153783928917054 58-59 26.592306405116428 24.707004029209756 25.766644719177396 22.93404484649642 60-61 27.51852768248866 24.248890604812328 25.73891116678746 22.493670545911556 62-63 26.967022151463997 24.144911335806107 25.558047148311868 23.33001936441802 64-65 25.320418716261127 24.199432609048046 25.921662155824414 24.558486518866406 66-67 25.936156130965855 24.354513320457766 24.91063667591676 24.798693872659623 68-69 24.71684642152023 25.460954414707604 26.00645269844368 23.815746465328484 70-71 23.100325670581366 26.575020921690296 27.214635888441514 23.110017519286828 72-73 22.46838457684577 27.248535009316825 27.104773194853067 23.178307218984337 74-75 22.02022328508903 29.431680095263165 27.03348463304242 21.514611986605388 76-77 21.65298633052944 31.436682592889564 25.8446842834024 21.065646793178598 78-79 21.45692977013474 33.690255546136754 24.140436055444926 20.712378628283577 80-81 21.361443154532772 34.651286390659116 23.609385864992603 20.37788458981551 82-83 21.53429998285278 35.07907190911006 23.187014591435535 20.199613516601627 84-85 21.76158249821569 35.00223052687183 22.930369545531843 20.30581742938064 86-87 22.31504384621032 34.36895150176792 22.974264695988133 20.341739956033624 88-89 23.150060213431907 33.288768292224816 23.069733605719723 20.491437888623558 90-91 23.898295996813662 32.1838967105817 23.13043489062038 20.78737240198426 92-93 24.33614914617541 31.59116543033757 23.227966037100224 20.8447193863868 94-95 24.807810117717512 31.19895251794878 23.152138787984068 20.841098576349633 96-97 25.119119694583524 30.473551636426276 23.323772741693453 21.083555927296743 98-99 25.415351454502694 30.223125705976077 23.311872112292466 21.049650727228762 100 26.219689019313204 30.262381485100658 22.561133888523347 20.95679560706279 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21147.0 1 15458.5 2 8672.5 3 7343.0 4 7008.5 5 6717.0 6 6425.0 7 6176.5 8 6099.0 9 6193.5 10 6357.0 11 7129.5 12 23265.5 13 28364.5 14 18433.0 15 19975.5 16 22738.5 17 26102.5 18 29634.0 19 33926.0 20 39613.5 21 47894.5 22 59383.0 23 73424.0 24 89260.0 25 106829.5 26 126843.5 27 152315.5 28 184892.0 29 224803.0 30 318067.5 31 442696.0 32 556705.0 33 674387.5 34 781952.0 35 864270.0 36 907224.5 37 897576.5 38 856244.0 39 806041.5 40 756907.5 41 721047.0 42 702326.5 43 697246.5 44 705158.5 45 726325.0 46 749448.0 47 774713.5 48 794829.0 49 814742.5 50 834132.0 51 851116.5 52 869113.0 53 872039.5 54 862876.0 55 851418.5 56 828404.0 57 783176.0 58 715198.0 59 632237.5 60 537560.5 61 442527.5 62 358187.5 63 283733.5 64 220087.0 65 167759.0 66 125053.0 67 92892.5 68 70188.0 69 54391.5 70 42776.5 71 33288.5 72 26554.0 73 20818.0 74 16008.5 75 12857.5 76 10082.0 77 7019.5 78 4636.5 79 3312.5 80 2354.0 81 1680.5 82 1192.5 83 872.0 84 645.5 85 471.5 86 366.5 87 285.0 88 207.5 89 167.5 90 148.0 91 115.0 92 89.0 93 62.5 94 42.0 95 43.0 96 35.0 97 24.5 98 17.5 99 11.0 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07862167153118826 2 0.0661311160798356 3 0.06376163401589335 4 0.05953041604456793 5 0.06195255326548666 6 0.05952665496192675 7 0.056149202750148765 8 0.05452817613180097 9 0.053038787405894426 10-11 0.05317606692229743 12-13 0.05467297781348633 14-15 0.053232483161915096 16-17 0.0571063982823286 18-19 0.058911717950094124 20-21 0.06507425185766454 22-23 0.06544471849782059 24-25 0.0636864123630698 26-27 0.06570047211742071 28-29 0.06400234330492877 30-31 0.06444615105658778 32-33 0.06747382258273621 34-35 0.06785745301213637 36-37 0.07053158277001405 38-39 0.07125935226108203 40-41 0.06983954356403727 42-43 0.06804550714419527 44-45 0.07021753236947567 46-47 0.06813953421022474 48-49 0.06959695373368127 50-51 0.0706105655054788 52-53 0.06972483054348133 54-55 0.07063689308396703 56-57 0.0708832439969642 58-59 0.06999750903496675 60-61 0.07017051883646094 62-63 0.07047892761303756 64-65 0.06921144276296051 66-67 0.07207550719421768 68-69 0.07106377596374075 70-71 0.07285405130094157 72-73 0.07330350067656234 74-75 0.07839036494875581 76-77 0.07899401871266491 78-79 0.07547928698448392 80-81 0.07453901632418938 82-83 0.07936072427017977 84-85 0.07663205881400502 86-87 0.07629544191761957 88-89 0.07870817643193537 90-91 0.07866116289892064 92-93 0.07847122822554115 94-95 0.08095354276871873 96-97 0.07980453202183879 98-99 0.08286793383307842 100 0.0820254513214545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6588089E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.796338267773045 #Duplication Level Percentage of deduplicated Percentage of total 1 73.72700173218134 30.815187038669407 2 12.755762375845233 10.662883182483196 3 4.904556153196958 6.149774640969233 4 2.4758779141330756 4.139305232352575 5 1.4430374590667494 3.015684088611078 6 0.9770073841034583 2.450119866966013 7 0.6737193242137295 1.971130054066075 8 0.46829084498583845 1.56582740517835 9 0.35025600684168484 1.317547668804704 >10 2.0309057369414325 16.01834824743584 >50 0.14717581626245152 4.142039260635707 >100 0.04299148051247703 3.0787279971671904 >500 0.001927706107425193 0.5442922055930017 >1k 0.0010545147792459558 0.9770356235906053 >5k 2.0870143876949051E-4 0.6160189404906066 >10k+ 2.2684938996509388E-4 12.536078546986456 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG 823816 3.0984400571248276 Illumina Single End PCR Primer 1 (100% over 32bp) ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA 618828 2.3274632486749987 Illumina Single End PCR Primer 1 (100% over 34bp) ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG 295623 1.1118625336330112 Illumina Single End PCR Primer 1 (100% over 30bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 285583 1.0741012639155827 No Hit ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA 210162 0.7904366500352845 Illumina Single End PCR Primer 1 (100% over 36bp) ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG 201346 0.757278945470658 Illumina Single End PCR Primer 1 (100% over 28bp) ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC 176823 0.6650459158610459 Illumina Single End PCR Primer 1 (100% over 38bp) ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA 139282 0.5238511124285766 Illumina Single End PCR Primer 1 (100% over 26bp) ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG 100194 0.3768379141502046 Illumina Single End PCR Primer 1 (100% over 24bp) ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC 90858 0.34172444661216533 Illumina Single End PCR Primer 1 (100% over 40bp) ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG 56395 0.21210625554924237 Illumina Single End PCR Primer 1 (100% over 22bp) ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 42638 0.16036504165455442 Illumina Single End PCR Primer 1 (100% over 37bp) ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT 41109 0.154614346296193 No Hit TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 32142 0.12088871825274843 Illumina Single End PCR Primer 1 (100% over 37bp) ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT 29028 0.10917670690811965 Illumina Single End PCR Primer 1 (100% over 39bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.0619692524724134E-4 3.7610826411781606E-6 0.0 0.0 3.7610826411781606E-6 2 6.393840490002873E-4 1.5044330564712642E-5 0.0 1.1283247923534482E-5 3.7610826411781606E-6 3 7.935884372885919E-4 1.5044330564712642E-5 0.0 1.1283247923534482E-5 3.7610826411781606E-6 4 9.064209165239367E-4 2.6327578488247126E-5 0.0 1.1283247923534482E-5 1.1283247923534482E-5 5 0.0012073075278181897 3.384974377060344E-5 0.0 1.1283247923534482E-5 1.1283247923534482E-5 6 0.001402883825159454 4.513299169413793E-5 0.0 1.5044330564712642E-5 1.1283247923534482E-5 7 0.0016887261058889942 4.889407433531609E-5 0.0 1.5044330564712642E-5 1.5044330564712642E-5 8 0.0019143910643596837 5.265515697649425E-5 0.0 1.8805413205890802E-5 1.5044330564712642E-5 9 0.05041355172235207 5.265515697649425E-5 0.0 1.8805413205890802E-5 1.5044330564712642E-5 10-11 0.26052643347177 6.205786357943965E-5 0.0 1.8805413205890802E-5 2.0685954526479883E-5 12-13 1.0011813936684204 7.146057018238505E-5 0.0 3.0088661129425285E-5 2.2566495847068964E-5 14-15 2.0775957234083275 7.710219414415229E-5 0.0 4.137190905295977E-5 3.0088661129425285E-5 16-17 3.9076482706222326 1.0154923131181035E-4 0.0 4.137190905295977E-5 3.7610826411781605E-5 18-19 7.001625803193303 1.2599626847946838E-4 0.0 4.513299169413793E-5 4.325245037354885E-5 20-21 9.428652431545569 1.5232384696771552E-4 0.0 4.513299169413793E-5 5.0774615655905175E-5 22-23 10.568854722879859 1.6548763621183907E-4 0.0 4.513299169413793E-5 5.8296780938261485E-5 24-25 11.401372246045964 1.9369575602067528E-4 0.0 4.513299169413793E-5 6.017732225885057E-5 26-27 12.013875837409753 2.10620627905977E-4 0.0 4.513299169413793E-5 6.017732225885057E-5 28-29 12.432300042323462 2.2378441715010055E-4 0.0 4.889407433531609E-5 6.205786357943965E-5 30-31 12.706249779741597 2.3318712375304597E-4 0.0 4.889407433531609E-5 6.393840490002873E-5 32-33 12.889094812342474 2.407092890354023E-4 0.0 4.889407433531609E-5 6.76994875412069E-5 34-35 12.999674403075753 2.501119956383477E-4 0.0 5.0774615655905175E-5 7.898273546474136E-5 36-37 13.065959723543877 2.670368675236494E-4 0.0 5.265515697649425E-5 9.778814867063218E-5 38-39 13.108736396963316 2.7267849148541665E-4 0.0 5.265515697649425E-5 9.778814867063218E-5 40-41 13.1424563833828 2.8396173940895117E-4 0.0 5.8296780938261485E-5 9.966868999122126E-5 42-43 13.1749540179439 2.952449873324856E-4 0.0 6.393840490002873E-5 1.0154923131181034E-4 44-45 13.21001106924232 3.027671526148419E-4 0.0 6.393840490002873E-5 1.1095193791475574E-4 46-47 13.248137163975944 3.2909473110308903E-4 0.0 6.769948754120689E-5 1.2223518583829023E-4 48-49 13.291084966655557 3.422585203472126E-4 0.0 7.146057018238505E-5 1.241157271588793E-4 50-51 13.351950567037743 3.573028509119253E-4 0.0 7.710219414415229E-5 1.2787680980005746E-4 52-53 13.420548953330192 3.629444748736925E-4 0.0 8.086327678533046E-5 1.335184337618247E-4 54-55 13.487861049359358 3.7610826411781605E-4 0.0 8.274381810591953E-5 1.3916005772359195E-4 56-57 13.566740355051467 4.024358426060632E-4 0.0 8.274381810591953E-5 1.429211403647701E-4 58-59 13.659120066884086 4.250023384531322E-4 0.0 8.274381810591953E-5 1.617265535706609E-4 60-61 13.766993558657036 4.381661276972557E-4 0.0 8.462435942650861E-5 1.711292601736063E-4 62-63 13.89166968712945 4.5509099958255747E-4 0.0 8.838544206768678E-5 1.8617359073831895E-4 64-65 14.035942936703725 4.7953803675021545E-4 0.0 9.402706602945401E-5 1.9181521470008618E-4 66-67 14.200725369920342 5.133877805208189E-4 0.0 9.402706602945401E-5 1.9745683866185344E-4 68-69 14.388662908417373 5.303126524061207E-4 0.0 9.402706602945401E-5 2.10620627905977E-4 70-71 14.602151361837251 5.491180656120114E-4 0.0 9.966868999122126E-5 2.1626225186774424E-4 72-73 14.842935872525477 5.773261854208477E-4 0.0 1.053103139529885E-4 2.1814279318833332E-4 74-75 15.114416459189677 6.092953878708621E-4 0.0 1.053103139529885E-4 2.1814279318833332E-4 76-77 15.416918455478315 6.29981342397342E-4 0.0 1.053103139529885E-4 2.2190387582951147E-4 78-79 15.753005039211356 6.393840490002873E-4 0.0 1.053103139529885E-4 2.313065824324569E-4 80-81 16.122490036798055 6.450256729620546E-4 0.0 1.0907139659416666E-4 2.3694820639422412E-4 82-83 16.52965167974276 6.694727101297126E-4 0.0 1.1471302055593389E-4 2.501119956383477E-4 84-85 16.974232333884544 6.826364993738361E-4 0.0 1.1847410319711206E-4 2.6139524356188213E-4 86-87 17.4580486021391 7.033224539003161E-4 1.8805413205890803E-6 1.2035464451770114E-4 2.7455903280600575E-4 88 17.84572407591986 7.108446191826723E-4 7.522165282356321E-6 1.2035464451770114E-4 2.896033633707184E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 99870 0.0 82.07339 8 GTGGTAT 99700 0.0 82.05525 6 AGTGGTA 100200 0.0 81.802704 5 TGGTATC 100145 0.0 81.59495 7 GTATCAA 102975 0.0 79.53213 9 AAGCAGT 110320 0.0 75.37036 1 CAGTGGT 111665 0.0 73.555534 4 GCAGTGG 112910 0.0 73.30395 3 AGCAGTG 118535 0.0 70.10366 2 CAACGCA 96750 0.0 43.973595 12-13 ATCAACG 97455 0.0 43.507683 10-11 TCAACGC 95435 0.0 43.151546 12-13 ACGCAGA 100225 0.0 42.462395 14-15 AACGCAG 99440 0.0 41.60898 14-15 AGAGTAC 98115 0.0 41.49998 18-19 TATCAAC 100670 0.0 40.757534 10-11 CGCAGAG 101685 0.0 40.579563 16-17 CAGAGTA 105450 0.0 38.091843 18-19 GCAGAGT 111375 0.0 37.825436 16-17 GTACATG 59460 0.0 36.847233 22-23 >>END_MODULE