FastQCFastQC Report
Thu 26 May 2016
SRR1660592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26588089
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11762854.424105094578253No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8666483.2595347488117703Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6371942.396539292462877Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG3204091.2050847279772534Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC2314160.8703747004908853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT2133860.802562380470443TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA1790550.6734406523161556TruSeq Adapter, Index 19 (100% over 37bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1693590.6369731950272921Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1300970.4893055683693552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA920360.3461550019634732TruSeq Adapter, Index 19 (100% over 39bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG777990.29260846840101973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC609530.22924927022773242No Hit
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG431670.16235465437173768TruSeq Adapter, Index 15 (97% over 36bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG363080.13655738853589666No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT335300.12610910095870373No Hit
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG326390.12275797632541399TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT318360.11973782696454792TruSeq Adapter, Index 19 (97% over 40bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA293260.11029750953519073TruSeq Adapter, Index 19 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1731150.085.685918
AGTGGTA1746500.085.024325
GTGGTAT1748600.084.85776
GTATCAA1749950.084.7331549
TGGTATC1755700.084.426217
AAGCAGT1805600.083.0782551
CAGTGGT1851450.080.45244
GCAGTGG1870800.079.836313
AGCAGTG1917900.078.147682
CAACGCA1715800.045.00882712-13
ATCAACG1728950.044.6711710-11
ACGCAGA1751400.044.0791914-15
AGAGTAC1744300.043.41826218-19
TCAACGC1716250.043.24446512-13
AACGCAG1745050.042.40687614-15
TATCAAC1765900.042.03447710-11
AGTACTT1477800.041.9630220-21
CGCAGAG1769550.041.72811516-17
GAGTACT1425950.041.3662520-21
GCAGAGT1867700.041.1658316-17
Produced by FastQC (version 0.11.5)