FastQCFastQC Report
Thu 26 May 2016
SRR1660591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32812852
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14848104.52508669468902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1556000.4742044367249759No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT331820.1011250104075074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2371600.085.565848
GTGGTAT2394050.084.804886
AGTGGTA2400650.084.7337955
GTATCAA2393850.084.5742659
TGGTATC2404800.084.20957
AAGCAGT2536700.081.324491
GCAGTGG2535900.081.041173
CAGTGGT2537300.080.3436054
AGCAGTG2648100.077.873412
CAACGCA2321050.045.0390112-13
ATCAACG2346400.044.62441610-11
ACGCAGA2364300.044.31231314-15
TCAACGC2323550.043.6894512-13
AGAGTAC2331500.043.5154218-19
AACGCAG2346900.043.18448314-15
CGCAGAG2382500.042.47108516-17
GAGTACT1905600.042.00590520-21
TATCAAC2423550.041.74900410-11
AGTACTT2013000.041.4977220-21
GCAGAGT2534900.040.89170516-17
Produced by FastQC (version 0.11.5)