FastQCFastQC Report
Thu 26 May 2016
SRR1660591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32812852
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11347363.4582059493030353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1704870.5195738547810475No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT341690.10413297813917546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1436300.081.101328
AGTGGTA1464200.079.851195
GTGGTAT1464650.079.669456
GTATCAA1463700.079.429039
TGGTATC1470000.079.162147
AAGCAGT1591150.074.594141
GCAGTGG1592600.074.0889743
CAGTGGT1611200.072.8662344
AGCAGTG1704650.069.722032
CAACGCA1387400.044.28624312-13
ATCAACG1407700.043.5925310-11
ACGCAGA1433700.042.94618614-15
TCAACGC1385450.041.91858712-13
AGAGTAC1465850.041.66935718-19
AACGCAG1416150.040.8804514-15
GAGTACT1417800.039.80793420-21
CGCAGAG1451150.039.777916-17
AGTACTT1534850.039.489820-21
TATCAAC1494750.038.897410-11
GCAGAGT1627350.037.70722616-17
Produced by FastQC (version 0.11.5)