FastQCFastQC Report
Thu 26 May 2016
SRR1660590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26804052
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10463293.903622482153071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA821520.30649097382739No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT305510.11397903570698938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1674300.083.7741248
AGTGGTA1691800.083.133795
GTATCAA1689950.082.827519
GTGGTAT1709100.082.200576
TGGTATC1703900.082.196267
AAGCAGT1784350.079.724491
GCAGTGG1803900.078.607913
CAGTGGT1802650.078.1452264
AGCAGTG1899350.074.915342
CAACGCA1635050.044.61998712-13
ATCAACG1662950.043.92685710-11
ACGCAGA1668200.043.8488714-15
AGAGTAC1650800.043.1021818-19
TCAACGC1633300.042.89431412-13
AACGCAG1651450.042.40163414-15
CGCAGAG1696000.041.23797616-17
GAGTACT1333750.041.07832720-21
AGTACTT1414050.040.90062320-21
GCAGAGT1813050.040.03736516-17
TATCAAC1751250.039.9332710-11
Produced by FastQC (version 0.11.5)