##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660590_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26804052 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06663432827246 34.0 31.0 34.0 30.0 34.0 2 32.20709555406026 34.0 31.0 34.0 30.0 34.0 3 32.21911071505159 34.0 31.0 34.0 30.0 34.0 4 35.60754832888699 37.0 35.0 37.0 35.0 37.0 5 35.57905498765634 37.0 35.0 37.0 33.0 37.0 6 35.60244327984441 37.0 35.0 37.0 35.0 37.0 7 35.56899303881369 37.0 35.0 37.0 33.0 37.0 8 35.57928849712722 37.0 35.0 37.0 33.0 37.0 9 37.266635805661025 39.0 38.0 39.0 34.0 39.0 10-11 37.203233171611515 39.0 37.0 39.0 34.0 39.0 12-13 37.23259626939986 39.0 38.0 39.0 34.0 39.0 14-15 38.64491827578905 41.0 39.0 41.0 34.5 41.0 16-17 38.608895625183834 41.0 39.0 41.0 34.0 41.0 18-19 38.54839697371129 41.0 38.5 41.0 34.0 41.0 20-21 38.494640362583986 40.0 38.5 41.0 34.0 41.0 22-23 38.34820966621017 40.0 38.5 41.0 34.0 41.0 24-25 38.342874689244745 40.0 38.0 41.0 34.0 41.0 26-27 38.32323456169985 40.0 38.0 41.0 34.0 41.0 28-29 38.22102622767632 40.0 38.0 41.0 34.0 41.0 30-31 38.12227002096549 40.0 38.0 41.0 33.5 41.0 32-33 38.02976730906208 40.0 38.0 41.0 33.0 41.0 34-35 37.918726560446906 40.0 38.0 41.0 33.0 41.0 36-37 37.75131981910795 40.0 38.0 41.0 33.0 41.0 38-39 37.40829263426291 40.0 37.0 41.0 32.5 41.0 40-41 37.291604884962915 40.0 36.5 41.0 32.0 41.0 42-43 37.14484360424312 40.0 36.0 41.0 32.0 41.0 44-45 37.00897588170624 40.0 36.0 41.0 31.5 41.0 46-47 36.84547388581398 40.0 35.5 41.0 31.0 41.0 48-49 36.668879373163435 39.5 35.0 41.0 31.0 41.0 50-51 35.45766220346088 38.0 34.0 40.0 29.5 40.5 52-53 35.4716660003495 38.5 35.0 39.5 29.5 40.5 54-55 35.8729299771542 39.0 35.0 40.5 29.0 41.0 56-57 35.87458769293538 39.0 35.0 41.0 29.0 41.0 58-59 35.73861336338253 39.0 35.0 41.0 28.5 41.0 60-61 35.51548463642736 39.0 35.0 41.0 28.0 41.0 62-63 35.236969824562344 38.5 35.0 41.0 28.0 41.0 64-65 34.94158381352193 37.5 35.0 40.5 28.0 41.0 66-67 34.62819576681913 37.0 35.0 40.0 28.0 41.0 68-69 34.29312245402299 36.5 35.0 39.5 27.0 41.0 70-71 33.95620223763183 36.0 34.0 39.0 26.0 41.0 72-73 33.59883729519701 35.5 34.0 39.0 26.0 41.0 74-75 33.240974517584135 35.0 34.0 37.5 26.0 40.0 76-77 32.89092449529646 35.0 34.0 37.0 26.0 39.0 78-79 32.55258841088653 35.0 34.0 37.0 25.0 39.0 80-81 32.2364851963427 35.0 34.0 36.0 24.5 39.0 82-83 31.86151000602446 35.0 33.0 36.0 23.5 37.0 84-85 31.613757968384782 35.0 33.0 35.5 22.5 37.0 86-87 31.33544650264072 35.0 33.0 35.0 20.0 36.5 88-89 31.157764729004406 35.0 33.0 35.0 19.5 36.0 90-91 30.976123759198796 35.0 33.0 35.0 18.5 36.0 92-93 30.807088999081184 35.0 33.0 35.0 16.0 36.0 94-95 30.592406793569868 35.0 33.0 35.0 4.5 35.5 96-97 30.44598889376875 35.0 33.0 35.0 2.0 35.0 98-99 30.289735857847162 35.0 33.0 35.0 2.0 35.0 100 30.16692961198553 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4294226389909426 1101 2 0.3911182715308854 1101 3 0.45365388702015963 1101 4 0.3960404405132607 1101 5 0.3571006803481964 1101 6 0.3798992787516653 1101 7 0.34047541072345666 1101 8 0.3825845509593435 1101 9 0.46194540576306764 1101 10-11 0.5015862489601162 1101 12-13 0.4561097384547921 1101 14-15 0.5473096825544417 1101 16-17 0.5591859879782461 1101 18-19 0.5113484937169943 1101 20-21 0.5239218671149786 1101 22-23 0.5154964438013181 1101 24-25 0.5597160203076257 1101 26-27 0.5278396086255412 1101 28-29 0.5106769054074292 1101 30-31 0.573872392631138 1101 32-33 0.527713492846658 1101 34-35 0.4913896748534867 1101 36-37 0.48245352587899504 1101 38-39 0.5292719546431783 1101 40-41 0.534895328768684 1101 42-43 0.5751046663970243 1101 44-45 0.5312167430674677 1101 46-47 0.5097696370598257 1101 48-49 0.5028815006351053 1101 50-51 0.47776993775698173 1101 52-53 0.49729108657705723 1101 54-55 0.5601352198520644 1101 56-57 0.6212328722370799 1101 58-59 0.612725392232015 1101 60-61 0.5958613151192438 1101 62-63 0.6011228849819332 1101 64-65 0.5591998509708063 1101 66-67 0.565599726541997 1101 68-69 0.5027014120729802 1101 70-71 0.45863122109089716 1101 72-73 0.4340471881423369 1101 74-75 0.44147638175272874 1101 76-77 0.4734831607664134 1101 78-79 0.3887928794953055 1101 80-81 0.4193645389212648 1101 82-83 0.3343317733810238 1101 84-85 0.317256806312912 1101 86-87 0.20768751270583508 1101 88-89 0.19851505717334916 1101 90-91 0.10724073096648468 1101 92-93 0.1400718368764906 1101 94-95 0.12386570814046038 1101 96-97 0.08255052414595809 1101 98-99 0.12109268185587041 1101 100 0.11006199772003455 1102 1 0.3245817937123121 1102 2 0.37336947396952525 1102 3 0.3769486761134857 1102 4 0.2899355320934802 1102 5 0.2763347117279764 1102 6 0.2889980457074799 1102 7 0.33333588960083915 1102 8 0.3271241332270236 1102 9 0.426083647570735 1102 10-11 0.3605537074894656 1102 12-13 0.41360051494736894 1102 14-15 0.4589907932987529 1102 16-17 0.46842839113238455 1102 18-19 0.42057038049022566 1102 20-21 0.4282632273378937 1102 22-23 0.427319274398414 1102 24-25 0.4905174865373283 1102 26-27 0.4796926975290674 1102 28-29 0.5040835212293615 1102 30-31 0.5194599611096322 1102 32-33 0.4636629342541809 1102 34-35 0.40838437035163366 1102 36-37 0.4799290815639381 1102 38-39 0.49142188747921267 1102 40-41 0.3703428362175316 1102 42-43 0.4523495072862289 1102 44-45 0.4174288716657131 1102 46-47 0.3732225606896833 1102 48-49 0.42024603287755014 1102 50-51 0.42973834113798404 1102 52-53 0.4775153983384257 1102 54-55 0.5210468884383772 1102 56-57 0.5821124725393645 1102 58-59 0.567405736753912 1102 60-61 0.5477050699128014 1102 62-63 0.5054726031987542 1102 64-65 0.5088255255280174 1102 66-67 0.4895678814153328 1102 68-69 0.4647573363181081 1102 70-71 0.3815059501146152 1102 72-73 0.40852259250058154 1102 74-75 0.3521044498613932 1102 76-77 0.38856734948605975 1102 78-79 0.4126397074467789 1102 80-81 0.3955902773049971 1102 82-83 0.2768145785939282 1102 84-85 0.2089590351849715 1102 86-87 0.23350746599983196 1102 88-89 0.225444448927 1102 90-91 0.27344863384339035 1102 92-93 0.26268086925750467 1102 94-95 0.30681389406175086 1102 96-97 0.2685768992903945 1102 98-99 0.27064666642915824 1102 100 0.2756078319250541 1103 1 0.3594069199641936 1103 2 0.3660593191142709 1103 3 0.366297630007935 1103 4 0.2795911511695408 1103 5 0.25688371091249707 1103 6 0.3068400183744089 1103 7 0.2463078192620216 1103 8 0.2528271292430162 1103 9 0.37350058552313925 1103 10-11 0.3746300714408406 1103 12-13 0.37827563391413577 1103 14-15 0.4206468649336301 1103 16-17 0.4336307575384808 1103 18-19 0.44042631000564825 1103 20-21 0.45923374243653825 1103 22-23 0.43180237926782894 1103 24-25 0.42432074706673717 1103 26-27 0.34973565027745224 1103 28-29 0.46199051666473423 1103 30-31 0.48539766894123915 1103 32-33 0.39933838770014063 1103 34-35 0.4174448369692527 1103 36-37 0.39707368186848413 1103 38-39 0.42954413791428436 1103 40-41 0.44887574019773524 1103 42-43 0.46399449912696866 1103 44-45 0.4372770750673496 1103 46-47 0.3994121309420393 1103 48-49 0.4268321483126627 1103 50-51 0.5304931397212727 1103 52-53 0.5362662289811269 1103 54-55 0.49221935286970364 1103 56-57 0.48325168459746237 1103 58-59 0.4747957509091023 1103 60-61 0.4600253070046705 1103 62-63 0.45974729785829993 1103 64-65 0.48439803910563484 1103 66-67 0.4774817643734579 1103 68-69 0.4523604021631229 1103 70-71 0.4474474885260804 1103 72-73 0.4121432152212421 1103 74-75 0.41817448277532066 1103 76-77 0.4194830313979878 1103 78-79 0.39219410964624757 1103 80-81 0.3535950473939664 1103 82-83 0.2535624614165357 1103 84-85 0.2227306022384461 1103 86-87 0.23792363400739092 1103 88-89 0.1689723586241847 1103 90-91 0.2344373042838228 1103 92-93 0.23831402428170279 1103 94-95 0.21292362450037317 1103 96-97 0.20350476918099503 1103 98-99 0.19383738664176065 1103 100 0.12478962621993972 1104 1 0.2316379079430888 1104 2 0.2878051410486222 1104 3 0.22552992751653278 1104 4 0.24959120289127412 1104 5 0.2067772772781069 1104 6 0.269536169016547 1104 7 0.26257915407395416 1104 8 0.2694678865526754 1104 9 0.33809340454199344 1104 10-11 0.3223309352261552 1104 12-13 0.31302932692688756 1104 14-15 0.3810344563073542 1104 16-17 0.3453936702738929 1104 18-19 0.38067908220986624 1104 20-21 0.31995102754969196 1104 22-23 0.2912960574170427 1104 24-25 0.35817648609437214 1104 26-27 0.3489533186163527 1104 28-29 0.4019249981559483 1104 30-31 0.39276185816908793 1104 32-33 0.3789448467105032 1104 34-35 0.3473399194282436 1104 36-37 0.36962114221811504 1104 38-39 0.3195506661671246 1104 40-41 0.3392904499240359 1104 42-43 0.2877312684645119 1104 44-45 0.34449444729930434 1104 46-47 0.29419550091699165 1104 48-49 0.31022332109332496 1104 50-51 0.26713960581751195 1104 52-53 0.3996211367434839 1104 54-55 0.4206543064833923 1104 56-57 0.3923781949933698 1104 58-59 0.37928113256864293 1104 60-61 0.4016641409122741 1104 62-63 0.2938181508176072 1104 64-65 0.31195546881983915 1104 66-67 0.3313268228626569 1104 68-69 0.3392141188708919 1104 70-71 0.2870603577990707 1104 72-73 0.3144138317475651 1104 74-75 0.3141969439088612 1104 76-77 0.3330203310126336 1104 78-79 0.29589408866662126 1104 80-81 0.31074229626634775 1104 82-83 0.21026572767648588 1104 84-85 0.21741360780144348 1104 86-87 0.17501330689004035 1104 88-89 0.16916583832356125 1104 90-91 0.14812784570372273 1104 92-93 0.12168875924821876 1104 94-95 0.12576035320403278 1104 96-97 0.131971741781733 1104 98-99 0.11560325883023737 1104 100 0.12138821091929586 1105 1 0.2623601811809735 1105 2 0.26120909302854045 1105 3 0.1974690627150153 1105 4 0.2554511809932123 1105 5 0.2462997519841963 1105 6 0.21918178480051864 1105 7 0.26076992545318944 1105 8 0.1775459794301142 1105 9 0.31323037971994694 1105 10-11 0.33199990283632275 1105 12-13 0.3139529972704267 1105 14-15 0.3812567392743844 1105 16-17 0.4429314567879459 1105 18-19 0.4166741233379696 1105 20-21 0.3255900230226487 1105 22-23 0.4118102559859622 1105 24-25 0.37589885886892915 1105 26-27 0.394965022781399 1105 28-29 0.3932340812136843 1105 30-31 0.38143973992401214 1105 32-33 0.3309878929516472 1105 34-35 0.3865384977003643 1105 36-37 0.3735546367559479 1105 38-39 0.3604769166058688 1105 40-41 0.38816115777942173 1105 42-43 0.4329835377882887 1105 44-45 0.4268782064325407 1105 46-47 0.38823309546747 1105 48-49 0.40257760465009795 1105 50-51 0.5139753875283546 1105 52-53 0.493503934936264 1105 54-55 0.4726085274742573 1105 56-57 0.45751050953522565 1105 58-59 0.46967975447224575 1105 60-61 0.4626037352517116 1105 62-63 0.4352029621956177 1105 64-65 0.4263263652710165 1105 66-67 0.4486885529596307 1105 68-69 0.41913151092038703 1105 70-71 0.44995326569920735 1105 72-73 0.35776245294019304 1105 74-75 0.3502361404009804 1105 76-77 0.2884273237255002 1105 78-79 0.31858152631587444 1105 80-81 0.32632863459672734 1105 82-83 0.3154757706269251 1105 84-85 0.24704807809797913 1105 86-87 0.20635142584159638 1105 88-89 0.2535444282856254 1105 90-91 0.2624007303437139 1105 92-93 0.22134478709154237 1105 94-95 0.24117176332429224 1105 96-97 0.1176285734023601 1105 98-99 0.15049707266373957 1105 100 0.12001514748749287 1106 1 0.2327992682463318 1106 2 0.25248016374077764 1106 3 0.25922374949620774 1106 4 0.17025032301674514 1106 5 0.16616197148447043 1106 6 0.2081406196014015 1106 7 0.2500235521478942 1106 8 0.25022627077792237 1106 9 0.3249415558201463 1106 10-11 0.3207757814264909 1106 12-13 0.3133085617314961 1106 14-15 0.34486595373519435 1106 16-17 0.30924917078105096 1106 18-19 0.34541477941775867 1106 20-21 0.39674329192835955 1106 22-23 0.4225648917800271 1106 24-25 0.3685258188806628 1106 26-27 0.40834619239804226 1106 28-29 0.4151267810512067 1106 30-31 0.39693040155689374 1106 32-33 0.3365470701015454 1106 34-35 0.4138303176170197 1106 36-37 0.39367267562575137 1106 38-39 0.38241383970326837 1106 40-41 0.38825413257825403 1106 42-43 0.43535620014215226 1106 44-45 0.43886911488903735 1106 46-47 0.43655985051083945 1106 48-49 0.40747600417414986 1106 50-51 0.43304339101955946 1106 52-53 0.47674632980730536 1106 54-55 0.5949813917331923 1106 56-57 0.5686031821339341 1106 58-59 0.5664805200582421 1106 60-61 0.5775071908293157 1106 62-63 0.558244108769216 1106 64-65 0.4959932065063981 1106 66-67 0.49551410323381617 1106 68-69 0.5388300876134338 1106 70-71 0.49441684153111964 1106 72-73 0.3976849674178169 1106 74-75 0.4513423563817085 1106 76-77 0.4617830734910271 1106 78-79 0.44783641555775233 1106 80-81 0.4478310406099908 1106 82-83 0.42326734630049145 1106 84-85 0.4232658985633613 1106 86-87 0.3618957755784358 1106 88-89 0.3200030501617057 1106 90-91 0.34102657803477854 1106 92-93 0.3704859372297342 1106 94-95 0.4121349403527894 1106 96-97 0.28676457902041363 1106 98-99 0.23842983955411867 1106 100 0.27106387685732614 1107 1 0.34252750816068556 1107 2 0.3488698911469612 1107 3 0.40036167045939663 1107 4 0.35408703834977473 1107 5 0.33844973439427406 1107 6 0.38287076064630554 1107 7 0.37124845818178187 1107 8 0.38551728000978613 1107 9 0.4953445986867919 1107 10-11 0.454127803501045 1107 12-13 0.41642815684087253 1107 14-15 0.5520610851077521 1107 16-17 0.526607784047151 1107 18-19 0.4969354147623193 1107 20-21 0.5313503870877341 1107 22-23 0.5647318564415826 1107 24-25 0.545082922372707 1107 26-27 0.5898516550891131 1107 28-29 0.592758977440937 1107 30-31 0.6246925082944799 1107 32-33 0.565801341790646 1107 34-35 0.6006763889494096 1107 36-37 0.6157854568974415 1107 38-39 0.6092112115355874 1107 40-41 0.6659284563990084 1107 42-43 0.6210754208270117 1107 44-45 0.6365340309915197 1107 46-47 0.6386357098133075 1107 48-49 0.6663973266681111 1107 50-51 0.658211011785319 1107 52-53 0.6373236485153697 1107 54-55 0.7108088611428443 1107 56-57 0.6557054186970817 1107 58-59 0.6216562562871886 1107 60-61 0.5612948263061313 1107 62-63 0.5458668812125467 1107 64-65 0.5781643201299786 1107 66-67 0.5812132674106678 1107 68-69 0.5483837382522623 1107 70-71 0.5300916423303619 1107 72-73 0.5403040432010755 1107 74-75 0.5740825503318305 1107 76-77 0.5385794124480512 1107 78-79 0.5048895928158075 1107 80-81 0.5167116725795111 1107 82-83 0.48012850990566847 1107 84-85 0.490096814719994 1107 86-87 0.46833228001780114 1107 88-89 0.4532335580424025 1107 90-91 0.44697277615946973 1107 92-93 0.5097066835920536 1107 94-95 0.5663363535744246 1107 96-97 0.46226924819510273 1107 98-99 0.445740332449688 1107 100 0.4753707856291953 1108 1 0.11074267291511575 1108 2 0.1467221900707827 1108 3 0.11010699540501889 1108 4 0.12179947214644216 1108 5 0.10600430915312131 1108 6 0.17895258042729978 1108 7 0.1795871337852688 1108 8 0.1681808802353899 1108 9 0.10378570091181416 1108 10-11 0.108189871887312 1108 12-13 0.16839164382894722 1108 14-15 0.1857674435360721 1108 16-17 0.20487038305368088 1108 18-19 0.20881639498190196 1108 20-21 0.1520539022990448 1108 22-23 0.2026716046618091 1108 24-25 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-0.9121451814773778 2316 58-59 -0.9889516593654619 2316 60-61 -1.4075681136926619 2316 62-63 -1.3020323199639563 2316 64-65 -1.0430911382275454 2316 66-67 -0.8951378394666918 2316 68-69 -0.8437864916121143 2316 70-71 -0.791752327431638 2316 72-73 -0.8433018911238932 2316 74-75 -0.794518146876463 2316 76-77 -0.7556180404452348 2316 78-79 -0.765659532208737 2316 80-81 -0.7532084350251012 2316 82-83 -0.7230623812515518 2316 84-85 -0.7064731565770508 2316 86-87 -0.682378466361186 2316 88-89 -0.6603351942354401 2316 90-91 -0.6938685873715755 2316 92-93 -0.6914703967914981 2316 94-95 -0.6083375697159603 2316 96-97 -0.7314848963962959 2316 98-99 -0.703949486704829 2316 100 -0.6506656970611573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 203803.0 3 40004.0 4 26771.0 5 25483.0 6 27063.0 7 28918.0 8 33002.0 9 39751.0 10 45586.0 11 50358.0 12 49275.0 13 48950.0 14 47518.0 15 46129.0 16 54303.0 17 69769.0 18 95840.0 19 150607.0 20 274806.0 21 306584.0 22 141906.0 23 121028.0 24 139168.0 25 158410.0 26 177825.0 27 205248.0 28 245546.0 29 299741.0 30 362941.0 31 451209.0 32 572783.0 33 751220.0 34 1051342.0 35 1706556.0 36 3100818.0 37 5957706.0 38 7431758.0 39 2258164.0 40 6163.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.912842741231286 36.871645429574585 17.56387696169323 30.651634867500903 2 21.272070789400015 31.713398310477796 29.853037626826783 17.161493273295406 3 25.6776082376508 26.526635731402386 25.114928416441522 22.680827614505287 4 20.016044662050994 26.584306708127954 25.57292291056924 27.82672571925181 5 22.465057269394237 32.34617919673907 25.0013184173087 20.187445116557996 6 27.362045829800124 25.621452227160248 25.460997580388295 21.55550436265133 7 22.24775814339119 25.98979201489014 30.650226291484113 21.112223550234557 8 25.48533136729287 25.046712392152738 27.461296941059437 22.00665929949496 9 25.84135423624716 24.583850750684785 28.163172138215064 21.41162287485299 10-11 20.97612972574433 28.607462296649288 28.955383594704703 21.461024382901677 12-13 21.17135743150036 25.9548207420066 28.234409038758056 24.639412787734983 14-15 21.42117529325876 31.61135080604371 25.037224269848444 21.93024963084909 16-17 24.931715857344933 25.682279634307342 24.721778345640928 24.6642261627068 18-19 21.97914704458036 28.52342930343969 24.599989643648094 24.897434008331857 20-21 24.809487718521073 28.572589258930602 24.88949722542977 21.728425797118554 22-23 24.484436572097046 25.767025587638905 28.04213423520675 21.706403605057304 24-25 21.629853306805284 28.27537465836966 25.324540878945513 24.77023115587954 26-27 21.610700305398435 25.992484198638238 30.620704193191106 21.776111302772218 28-29 22.761672721069313 25.574284451447575 29.871347330443328 21.792695497039784 30-31 22.535031378734686 25.634637094960077 29.774181400430084 22.05615012587516 32-33 22.21328747372985 25.87041635398628 29.783229001913362 22.133067170370506 34-35 22.204705755457624 25.858767708219165 29.720093183402156 22.21643335292105 36-37 22.214056764529854 25.828724936412307 29.664095858342527 22.293122440715315 38-39 22.308988687011812 25.796034511688497 29.851830801757078 22.043145999542613 40-41 22.157667586763715 25.937747959809776 29.836993811737596 22.06759064168891 42-43 22.100089970838777 25.81931243239624 29.852007478776937 22.228590117988045 44-45 22.127810553311544 25.835768639207917 29.939734200156337 22.096686607324198 46-47 22.13725595799564 25.880534033772136 29.767909219586603 22.214300788645623 48-49 22.210418152968185 25.857013784011336 29.626805566805036 22.305762496215443 50-51 22.248718371375205 25.943919631603503 29.679082120174797 22.128279876846488 52-53 22.358399315381387 26.10789652243183 29.319370475669178 22.21433368651761 54-55 22.50512326744775 26.220010640761743 28.811775626856186 22.463090464934325 56-57 22.63499813304002 26.68498602853316 28.26077274020963 22.41924309821719 58-59 22.722752373171467 26.978243836580702 27.854111505986584 22.444892284261243 60-61 22.67773300052677 27.00705307091923 27.732750182023054 22.58246374653095 62-63 22.553594568372603 27.052666060707338 27.898955425373828 22.49478394554623 64-65 22.60272873483585 27.101242637066996 27.820982070375806 22.475046557721345 66-67 22.54767904408928 26.88065633104578 27.997083122598344 22.574581502266597 68-69 22.437668493196753 26.840680866645865 28.307893935459283 22.4137567046981 70-71 22.525587412597343 26.792805791521573 28.298859350138866 22.382747445742222 72-73 22.469360405505686 26.676574197170755 28.363104933799242 22.490960463524317 74-75 22.51090526099001 26.71515533395205 28.4286713338709 22.34526807118704 76-77 22.622843819970715 26.85402559305193 28.101043280612316 22.42208730636504 78-79 22.616982259589662 26.95866137783589 27.897781200828316 22.526575161746134 80-81 22.662072416469993 27.090791187861264 27.80435095837095 22.442785437297793 82-83 22.75939948616415 27.158274812973342 27.54733653056573 22.53498917029678 84-85 22.66154321497297 27.194620745235397 27.490916679344597 22.652919360447036 86-87 22.689265080162535 27.247439018450788 27.47535322861987 22.587942672766808 88-89 22.821703445621925 27.305106863566813 27.3501485268254 22.52304116398586 90-91 22.827955091788844 27.29519477536074 27.221363346142258 22.65548678670816 92-93 22.853146674882836 27.317771427099807 27.296669711598177 22.532412186419183 94-95 22.97002255416219 27.34810535089326 27.159735124541566 22.522136970402983 96-97 22.886225354547985 27.31501365691389 27.129522191507743 22.669238797030385 98-99 22.948897109244307 27.274678388624903 27.165471688263963 22.610952813866824 100 23.133495759170625 27.30188248346705 26.893874209525137 22.670747547837188 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61599.0 1 37628.0 2 11088.5 3 7974.0 4 7135.5 5 6803.5 6 6954.0 7 7071.0 8 7119.5 9 7536.0 10 8286.0 11 14716.5 12 143891.5 13 158700.0 14 47851.0 15 47631.0 16 52942.5 17 58210.0 18 62378.5 19 66300.0 20 71902.0 21 81186.0 22 94405.0 23 110174.0 24 129434.0 25 152056.0 26 176944.0 27 208528.0 28 244366.5 29 280908.5 30 318306.5 31 353123.0 32 388487.5 33 428521.5 34 470632.5 35 515482.5 36 570673.5 37 616832.5 38 657960.5 39 701532.5 40 721719.5 41 745832.5 42 787274.5 43 814982.5 44 844727.5 45 886882.0 46 917557.0 47 933013.5 48 945011.0 49 945864.5 50 941920.0 51 946858.5 52 952685.0 53 946272.0 54 921605.0 55 893167.0 56 855415.0 57 794901.0 58 717822.5 59 630932.0 60 524448.0 61 420117.5 62 335414.5 63 252742.0 64 184987.5 65 137238.5 66 98279.5 67 70301.5 68 52413.0 69 41078.0 70 34263.0 71 29959.5 72 26511.5 73 22124.5 74 17234.5 75 14207.5 76 11382.0 77 8109.0 78 5456.5 79 3532.5 80 2276.0 81 1580.5 82 1058.5 83 694.5 84 467.0 85 306.5 86 209.0 87 161.0 88 118.0 89 88.0 90 80.0 91 58.0 92 51.0 93 42.0 94 33.5 95 33.0 96 28.0 97 17.0 98 13.5 99 13.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023444216568450173 2 0.03851283380587383 3 0.05834192531785866 4 0.13531909279984983 5 0.1808942916541126 6 0.11008783298883318 7 0.12329852217866165 8 0.15026086354406415 9 0.13614359500571033 10-11 0.1345803985158662 12-13 0.13461584091838055 14-15 0.1477071451734238 16-17 0.13818619662430143 18-19 0.14128460875989943 20-21 0.1505649220498453 22-23 0.15993290865127407 24-25 0.1564614185944722 26-27 0.14739375971961255 28-29 0.1663908874673128 30-31 0.15899088690023433 32-33 0.15482920268920533 34-35 0.14224155362778731 36-37 0.15007432458346223 38-39 0.145407119789202 40-41 0.13987250882814287 42-43 0.1444296556356479 44-45 0.1375930027295873 46-47 0.14593129426849344 48-49 0.13948637317969687 50-51 0.14217253421236462 52-53 0.1577765182667158 54-55 0.1597146580673698 56-57 0.15405506600270735 58-59 0.1520758876307209 60-61 0.15280525496667444 62-63 0.1467296810198697 64-65 0.14608798699539904 66-67 0.15877077092672406 68-69 0.153342487173208 70-71 0.14600031368391614 72-73 0.15010790159637058 74-75 0.14973482367516672 76-77 0.1140667090184723 78-79 0.03204552804180503 80-81 0.0404845506194362 82-83 0.17050034076937323 84-85 0.04509765911512185 86-87 0.1878260794300802 88-89 0.041338899058993024 90-91 0.17844876588062134 92-93 0.1876693867031746 94-95 0.24529873319153386 96-97 0.2719849968952455 98-99 0.3000180718945031 100 0.3170640021143072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6804052E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.888944826961406 #Duplication Level Percentage of deduplicated Percentage of total 1 69.0046327774037 27.525219896625885 2 14.866482646072754 11.860166120803505 3 5.88361325107543 7.040733730659805 4 3.0241892086705064 4.825268659637996 5 1.6943410109355543 3.379273755163317 6 1.152909539863974 2.759300701566691 7 0.7772928722823516 2.1703774747802753 8 0.5756128262552888 1.836847061455083 9 0.4112410130472058 1.4763573072021337 >10 2.2730664321276244 16.6825987061354 >50 0.2141830592817883 5.936043267857005 >100 0.11602750727992812 8.09021361232164 >500 0.005524057481314903 1.4268131746478327 >1k 8.183064693055369E-4 0.5679388135672458 >5k 3.742385899887246E-5 0.09734537367462265 >10k+ 2.8067894248418422E-5 4.3255023439015075 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1046329 3.903622482153071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82152 0.30649097382739 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 30551 0.11397903570698938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3057727242134882E-4 1.1192337636115613E-5 0.0 0.0 0.0 2 2.2011597351027375E-4 2.6115454484269768E-5 0.0 0.0 0.0 3 3.021931161751216E-4 5.596168818057807E-5 0.0 0.0 0.0 4 4.4769350544462456E-4 8.207714266484784E-5 0.0 3.730779212038538E-6 3.730779212038538E-6 5 5.745399986539348E-4 1.0073103872504053E-4 0.0 3.730779212038538E-6 3.730779212038538E-6 6 6.93924933439168E-4 1.3430805163338737E-4 0.0 7.461558424077076E-6 3.730779212038538E-6 7 7.722712968919774E-4 1.4550038926950298E-4 0.0 7.461558424077076E-6 7.461558424077076E-6 8 8.618099979809023E-4 1.566927269056186E-4 0.0 7.461558424077076E-6 7.461558424077076E-6 9 9.811949327661355E-4 1.6042350611765715E-4 0.0 7.461558424077076E-6 7.461558424077076E-6 10-11 0.0012255609711546598 1.921351294199847E-4 0.0 1.3057727242134884E-5 7.461558424077076E-6 12-13 0.0015370810353598778 2.1825058390425448E-4 0.0 1.865389606019269E-5 7.461558424077076E-6 14-15 0.0019437359694720784 2.443660383885242E-4 0.0 1.865389606019269E-5 7.461558424077076E-6 16-17 0.002454852721521358 2.760776616908518E-4 0.0 1.865389606019269E-5 7.461558424077076E-6 18-19 0.003141316096536449 3.376355186894877E-4 0.0 1.865389606019269E-5 1.3057727242134884E-5 20-21 0.0038184525235214436 3.861356484459887E-4 0.0 1.865389606019269E-5 1.6788506454173422E-5 22-23 0.004450819599961976 4.215780509603548E-4 1.865389606019269E-6 1.865389606019269E-5 2.6115454484269768E-5 24-25 0.0051689945982793944 4.327703885964704E-4 3.730779212038538E-6 1.865389606019269E-5 3.7307792120385385E-5 26-27 0.005844265635658369 4.607512326867594E-4 3.730779212038538E-6 1.865389606019269E-5 4.103857133242392E-5 28-29 0.006347920829283572 4.7753973914093284E-4 3.730779212038538E-6 1.865389606019269E-5 4.8500129756500995E-5 30-31 0.006944845503209739 5.12982141655299E-4 3.730779212038538E-6 1.865389606019269E-5 5.596168818057807E-5 32-33 0.007441039138410864 5.596168818057807E-4 3.730779212038538E-6 2.0519285666211956E-5 6.155785699863588E-5 34-35 0.007991329072186549 5.969246739261662E-4 3.730779212038538E-6 2.6115454484269768E-5 6.715402581669369E-5 36-37 0.008580792187688637 6.342324660465515E-4 3.730779212038538E-6 2.9846233696308306E-5 7.461558424077076E-5 38-39 0.009218755432947229 6.510209725007249E-4 3.730779212038538E-6 2.9846233696308306E-5 7.461558424077076E-5 40-41 0.00982314166529747 6.696748685609175E-4 3.730779212038538E-6 2.9846233696308306E-5 7.83463634528093E-5 42-43 0.01040887400158752 7.088480502873223E-4 5.5961688180578075E-6 3.171162330232757E-5 8.580792187688638E-5 44-45 0.011080414259754458 7.405596735896498E-4 7.461558424077076E-6 3.3577012908346843E-5 8.580792187688638E-5 46-47 0.011751954517921395 7.704059072859581E-4 7.461558424077076E-6 3.3577012908346843E-5 9.513486990698271E-5 48-49 0.012444014061754544 8.002521409822664E-4 7.461558424077076E-6 3.3577012908346843E-5 9.700025951300199E-5 50-51 0.013287170163675253 8.24502205860517E-4 9.326948030096344E-6 4.103857133242392E-5 1.0073103872504053E-4 52-53 0.014361634576742353 8.431561019207096E-4 1.1192337636115613E-5 4.290396093844319E-5 1.1005798675513688E-4 54-55 0.015656214963319724 8.87925452465172E-4 1.1192337636115613E-5 4.476935054446245E-5 1.1938493478523322E-4 56-57 0.01724925768686018 9.476179198577887E-4 1.1192337636115613E-5 5.2230908968539536E-5 1.1938493478523322E-4 58-59 0.019153820474605857 9.886564911902125E-4 1.1192337636115613E-5 5.9692467392616605E-5 1.2125032439125249E-4 60-61 0.021606807806521192 0.001018502724886521 1.1192337636115613E-5 6.528863621067441E-5 1.4550038926950298E-4 62-63 0.02472200844857337 0.0010446181793707906 1.1192337636115613E-5 6.715402581669369E-5 1.622888957236764E-4 64-65 0.028626268893971702 0.0010651374650370027 1.1192337636115613E-5 7.088480502873223E-5 1.678850645417342E-4 66-67 0.03376728264816081 0.001089387529915253 1.1192337636115613E-5 7.088480502873223E-5 1.6975045414775348E-4 68-69 0.04011893425665642 0.0011210991532175805 1.3057727242134884E-5 7.088480502873223E-5 1.7161584375377276E-4 70-71 0.04848334125004682 0.0011584069453379662 1.4923116848154153E-5 7.275019463475149E-5 1.7161584375377276E-4 72-73 0.059125388952386754 0.001206907075094467 1.4923116848154153E-5 7.461558424077076E-5 1.8467357099590762E-4 74-75 0.07258044418060375 0.0012498110360329101 1.4923116848154153E-5 7.648097384679002E-5 1.9026973981396545E-4 76-77 0.08984649037391809 0.0012796572697292186 1.4923116848154153E-5 8.580792187688638E-5 1.9773129823804253E-4 78-79 0.1118711454521876 0.0013169650618496038 1.4923116848154153E-5 9.140409069494418E-5 2.0892363587415814E-4 80-81 0.13920283395958194 0.0013505420747579508 1.4923116848154153E-5 9.513486990698271E-5 2.1451980469221595E-4 82-83 0.17413598511150477 0.0013897152564843556 1.4923116848154153E-5 9.886564911902127E-5 2.1825058390425448E-4 84-85 0.21826177624189058 0.0014120999317565866 1.4923116848154153E-5 1.0073103872504053E-4 2.2571214232833156E-4 86-87 0.2742178682536506 0.0014531385030890106 1.4923116848154153E-5 1.0073103872504053E-4 2.275775319343508E-4 88 0.32315636456756613 0.0014736577887552226 1.4923116848154153E-5 1.0073103872504053E-4 2.3130831114638936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 167430 0.0 83.774124 8 AGTGGTA 169180 0.0 83.13379 5 GTATCAA 168995 0.0 82.82751 9 GTGGTAT 170910 0.0 82.20057 6 TGGTATC 170390 0.0 82.19626 7 AAGCAGT 178435 0.0 79.72449 1 GCAGTGG 180390 0.0 78.60791 3 CAGTGGT 180265 0.0 78.145226 4 AGCAGTG 189935 0.0 74.91534 2 CAACGCA 163505 0.0 44.619987 12-13 ATCAACG 166295 0.0 43.926857 10-11 ACGCAGA 166820 0.0 43.84887 14-15 AGAGTAC 165080 0.0 43.10218 18-19 TCAACGC 163330 0.0 42.894314 12-13 AACGCAG 165145 0.0 42.401634 14-15 CGCAGAG 169600 0.0 41.237976 16-17 GAGTACT 133375 0.0 41.078327 20-21 AGTACTT 141405 0.0 40.900623 20-21 GCAGAGT 181305 0.0 40.037365 16-17 TATCAAC 175125 0.0 39.93327 10-11 >>END_MODULE