FastQCFastQC Report
Thu 26 May 2016
SRR1660590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26804052
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7885552.9419246015490494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA891920.3327556594801413No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319170.11907528011063401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1027450.079.2184758
AGTGGTA1049700.077.906385
GTATCAA1044900.077.6256949
TGGTATC1064750.076.3947757
GTGGTAT1067850.076.375416
AAGCAGT1138700.072.987491
GCAGTGG1165500.070.899623
CAGTGGT1165550.070.477314
AGCAGTG1265150.065.8427662
CAACGCA995100.043.76549512-13
ATCAACG1011300.043.03189510-11
ACGCAGA1032350.042.40934414-15
AGAGTAC1049250.041.29681418-19
TCAACGC992400.040.82114412-13
AACGCAG1015450.039.82512714-15
AGTACTT1098250.039.0170220-21
GAGTACT1012100.038.8784720-21
CGCAGAG1055250.038.28745316-17
TATCAAC1099400.036.9507710-11
GTACTTT1073400.036.7813122-23
Produced by FastQC (version 0.11.5)