##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26804052
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50795823706058	34.0	31.0	34.0	31.0	34.0
2	32.78707991612612	34.0	31.0	34.0	31.0	34.0
3	32.867590243445285	34.0	31.0	34.0	31.0	34.0
4	36.28641247972508	37.0	37.0	37.0	35.0	37.0
5	36.20554295298338	37.0	37.0	37.0	35.0	37.0
6	36.18714722684466	37.0	37.0	37.0	35.0	37.0
7	36.14546722264231	37.0	36.0	37.0	35.0	37.0
8	36.15779961179004	37.0	37.0	37.0	35.0	37.0
9	37.928629298286694	39.0	38.0	39.0	35.0	39.0
10-11	37.87749242166819	39.0	38.0	39.0	35.0	39.0
12-13	37.892878341677594	39.0	38.0	39.0	35.0	39.0
14-15	39.393206967364485	41.0	39.0	41.0	36.0	41.0
16-17	39.37054280076758	41.0	39.0	41.0	36.0	41.0
18-19	39.34238273004395	41.0	39.0	41.0	36.0	41.0
20-21	39.29637028386604	41.0	39.0	41.0	36.0	41.0
22-23	39.202838641709846	41.0	39.0	41.0	36.0	41.0
24-25	39.20979352300913	41.0	39.0	41.0	36.0	41.0
26-27	39.164214052412675	41.0	39.0	41.0	36.0	41.0
28-29	39.094383920013286	41.0	39.0	41.0	35.0	41.0
30-31	39.000157886576254	40.5	39.0	41.0	35.0	41.0
32-33	38.9411620862398	40.0	39.0	41.0	35.0	41.0
34-35	38.809162790013986	40.0	38.0	41.0	35.0	41.0
36-37	38.69907943396021	40.0	38.0	41.0	35.0	41.0
38-39	38.47225305711241	40.0	38.0	41.0	34.0	41.0
40-41	38.36119736672649	40.0	38.0	41.0	34.0	41.0
42-43	38.26771616470525	40.0	38.0	41.0	34.0	41.0
44-45	38.174626470654516	40.0	37.0	41.0	34.0	41.0
46-47	38.04421863157108	40.0	37.0	41.0	33.0	41.0
48-49	37.89608352871424	40.0	37.0	41.0	33.0	41.0
50-51	37.73574213704704	40.0	36.5	41.0	33.0	41.0
52-53	37.47306871737153	40.0	36.0	41.0	33.0	41.0
54-55	37.041281556982504	39.5	36.0	41.0	31.5	41.0
56-57	36.633903224781086	39.0	35.0	41.0	31.0	41.0
58-59	36.580490479573754	39.0	35.0	41.0	31.0	41.0
60-61	36.37070460466201	39.0	35.0	41.0	31.0	41.0
62-63	36.059758707377526	38.5	35.0	40.5	31.0	41.0
64-65	35.94358886484775	38.0	35.0	40.0	31.0	41.0
66-67	35.69909732304653	37.0	35.0	40.0	31.0	41.0
68-69	35.38309881282129	37.0	35.0	39.5	31.0	41.0
70-71	35.03700382688409	36.0	35.0	39.0	30.0	41.0
72-73	34.68517295444734	36.0	35.0	39.0	30.0	41.0
74-75	34.325765186547166	35.0	34.0	37.5	30.0	40.0
76-77	32.76716344976498	34.5	32.0	36.0	27.5	39.0
78-79	33.65770669673376	35.0	34.0	37.0	29.5	39.0
80-81	33.576162682418314	35.0	34.0	36.0	30.0	39.0
82-83	33.341783510940814	35.0	34.0	36.0	30.0	37.0
84-85	33.11823663825156	35.0	34.0	36.0	29.0	37.0
86-87	32.91858036986348	35.0	34.0	35.0	29.0	37.0
88-89	32.744765847342784	35.0	34.0	35.0	29.0	36.0
90-91	32.58003957759819	35.0	34.0	35.0	29.0	36.0
92-93	32.417726357194056	35.0	34.0	35.0	29.0	36.0
94-95	32.294515788135314	35.0	34.0	35.0	29.0	36.0
96-97	32.13036737878288	35.0	34.0	35.0	28.5	35.0
98-99	31.99217832811248	35.0	34.0	35.0	27.0	35.0
100	31.90118311216528	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5131821846075937
1101	2	0.36275086640542753
1101	3	0.31745066212494066
1101	4	0.218013312300414
1101	5	0.21829697002855397
1101	6	0.18603958004088383
1101	7	0.22815688528496736
1101	8	0.20233896620835168
1101	9	0.3126422058556244
1101	10-11	0.2791566370985734
1101	12-13	0.30328178677781636
1101	14-15	0.3785957933158244
1101	16-17	0.38507140747217505
1101	18-19	0.34116718860412476
1101	20-21	0.3215836853791245
1101	22-23	0.21855944921640713
1101	24-25	0.34511008471136506
1101	26-27	0.3894369313997217
1101	28-29	0.42518581271962574
1101	30-31	0.3707747165055011
1101	32-33	0.39673088583761995
1101	34-35	0.4380018878117582
1101	36-37	0.3946624733540247
1101	38-39	0.34758913658158264
1101	40-41	0.034666572735496004
1101	42-43	0.12136604444034305
1101	44-45	0.3055935979699882
1101	46-47	0.3129622510421868
1101	48-49	0.3372120920406232
1101	50-51	0.38044298575736235
1101	52-53	0.3229378889971031
1101	54-55	0.21823819223640584
1101	56-57	0.20558461698632158
1101	58-59	0.20082517817533585
1101	60-61	0.25721053858475074
1101	62-63	0.2242541344770288
1101	64-65	0.2748073470121426
1101	66-67	0.240189565017495
1101	68-69	0.24801633984297666
1101	70-71	0.2860582244743384
1101	72-73	0.2238004809348766
1101	74-75	0.1697329109543162
1101	76-77	0.1088608023512947
1101	78-79	0.24266128406285503
1101	80-81	0.2993620203187817
1101	82-83	0.2621507102924099
1101	84-85	0.2802031572740873
1101	86-87	0.2720141205718676
1101	88-89	0.2558737992553404
1101	90-91	0.24941532428722013
1101	92-93	0.19249591470616423
1101	94-95	0.2414783709542121
1101	96-97	0.1804971445293262
1101	98-99	0.2590899982507935
1101	100	0.26133617188302694
1102	1	0.4890507793446659
1102	2	0.32704541597708925
1102	3	0.2527119811741372
1102	4	0.176379923296885
1102	5	0.2269543660040796
1102	6	0.23593335851529673
1102	7	0.21399899627034102
1102	8	0.2537751300974378
1102	9	0.30253270084782
1102	10-11	0.2599253130054464
1102	12-13	0.3083897627284955
1102	14-15	0.3516398669950149
1102	16-17	0.3263184353781554
1102	18-19	0.2948404433078693
1102	20-21	0.26849251309443645
1102	22-23	0.3022149690394116
1102	24-25	0.3637585742018814
1102	26-27	0.39152175384836596
1102	28-29	0.3068037935873491
1102	30-31	0.3759796258066572
1102	32-33	0.3332596126609957
1102	34-35	0.4339238076265204
1102	36-37	0.44023230881789743
1102	38-39	0.3881206879116661
1102	40-41	0.3877456541000228
1102	42-43	0.3675490896601019
1102	44-45	0.32275581795422426
1102	46-47	0.32015548725828324
1102	48-49	0.30563902184292147
1102	50-51	0.29420340317674487
1102	52-53	0.33577196723619807
1102	54-55	0.34381439140290126
1102	56-57	0.21614208032246296
1102	58-59	0.1565347976241327
1102	60-61	0.14057833671062525
1102	62-63	0.11568217880292053
1102	64-65	0.1672620429529914
1102	66-67	0.17544083581296377
1102	68-69	0.15303736394778866
1102	70-71	0.10354677513517885
1102	72-73	0.035326103565594735
1102	74-75	0.0977473576444936
1102	76-77	-0.16491020434850157
1102	78-79	0.15994632744101978
1102	80-81	0.15164107407645133
1102	82-83	-0.012230577097319895
1102	84-85	0.10521594798333922
1102	86-87	0.17713094400995288
1102	88-89	0.21014598012229868
1102	90-91	0.207719022449389
1102	92-93	0.20432604320639314
1102	94-95	0.19449384184854068
1102	96-97	0.29782523462645116
1102	98-99	0.3016334540054544
1102	100	0.34019428574599075
1103	1	0.4245745445629723
1103	2	0.2921657009708625
1103	3	0.2647595838806822
1103	4	0.1777693349519538
1103	5	0.17322767160408148
1103	6	0.18492974659129402
1103	7	0.21560020600199437
1103	8	0.20179925512702113
1103	9	0.2373123284942622
1103	10-11	0.2316218301622328
1103	12-13	0.2427106147643272
1103	14-15	0.2507102343197687
1103	16-17	0.2880149101438789
1103	18-19	0.2960996511432725
1103	20-21	0.30105289489573295
1103	22-23	0.30496288105715763
1103	24-25	0.3124817740737882
1103	26-27	0.2838366927406213
1103	28-29	0.27214473775811143
1103	30-31	0.32836513522727984
1103	32-33	0.3201490881419744
1103	34-35	0.3808857731990969
1103	36-37	0.32221864549335066
1103	38-39	0.26917126913763667
1103	40-41	0.2790953501116391
1103	42-43	0.27087745503390437
1103	44-45	0.2794994589352555
1103	46-47	0.2704737245173945
1103	48-49	0.3353081021338582
1103	50-51	0.32535930740110786
1103	52-53	0.2864288270714326
1103	54-55	0.2706081112757559
1103	56-57	0.2625391285230947
1103	58-59	0.2483476878313411
1103	60-61	0.2804067780122992
1103	62-63	0.24848779292538126
1103	64-65	0.2483315087154665
1103	66-67	0.24247557830057076
1103	68-69	0.24483987347159086
1103	70-71	0.16085628215920877
1103	72-73	0.19030311222355323
1103	74-75	0.21350397015520173
1103	76-77	0.24598266228107235
1103	78-79	0.25577367389083605
1103	80-81	0.28513066464233816
1103	82-83	0.276586242419917
1103	84-85	0.24987168568601703
1103	86-87	0.25159976514104443
1103	88-89	0.21010298908520753
1103	90-91	0.20748419474207225
1103	92-93	0.19398131870969593
1103	94-95	0.21196375769475395
1103	96-97	0.23673046173897916
1103	98-99	0.26211511361298534
1103	100	0.2780053806231457
1104	1	0.37700606417666194
1104	2	0.25816341163753975
1104	3	0.17374400497060805
1104	4	0.14960198475021969
1104	5	0.1337396295077582
1104	6	0.16525051509870536
1104	7	0.15640842924076281
1104	8	0.14216858711170488
1104	9	0.20463290336842022
1104	10-11	0.15463647517172063
1104	12-13	0.18611788755154635
1104	14-15	0.22565148974206295
1104	16-17	0.23894800258322846
1104	18-19	0.24739307436897917
1104	20-21	0.24020412343242015
1104	22-23	0.27945376946525613
1104	24-25	0.2560552514262895
1104	26-27	0.23265617644779013
1104	28-29	0.21846724152975838
1104	30-31	0.30377284797251747
1104	32-33	0.25792149601828385
1104	34-35	0.34341144415123637
1104	36-37	0.23086525899653054
1104	38-39	0.31774058127410854
1104	40-41	0.3207599963979959
1104	42-43	0.3477930099257023
1104	44-45	0.2723115115220409
1104	46-47	0.2841353825804802
1104	48-49	0.34973933921756384
1104	50-51	0.33322106780857297
1104	52-53	0.2789684515780948
1104	54-55	0.3016625768496084
1104	56-57	0.20193924366861182
1104	58-59	0.2317120784506983
1104	60-61	0.23987579944029136
1104	62-63	0.19141347978195
1104	64-65	0.20875315561958274
1104	66-67	0.2352161094246057
1104	68-69	0.20209435604895987
1104	70-71	0.22888921888381475
1104	72-73	0.24918948829812138
1104	74-75	0.2782607632394942
1104	76-77	-0.03449240671088205
1104	78-79	0.2912243657821918
1104	80-81	0.32190220036088135
1104	82-83	0.2832729173168005
1104	84-85	0.2236459856080799
1104	86-87	0.14518307770487837
1104	88-89	0.22244748041324414
1104	90-91	0.23688078472803653
1104	92-93	0.24213391528630268
1104	94-95	0.28745481516826743
1104	96-97	0.3270706275136135
1104	98-99	0.35651051399901945
1104	100	0.37988924787026335
1105	1	0.3973376260566681
1105	2	0.28779502867432427
1105	3	0.2026756846481419
1105	4	0.15829224684598842
1105	5	0.12730271292922168
1105	6	0.13422643246381227
1105	7	0.1297497217655561
1105	8	0.1295988459594355
1105	9	0.1976666275889869
1105	10-11	0.20754167360517783
1105	12-13	0.2371126180953098
1105	14-15	0.2706190224525784
1105	16-17	0.26556221791621937
1105	18-19	0.23250242625055506
1105	20-21	0.2219201168794669
1105	22-23	0.270361571942594
1105	24-25	0.19877208607043428
1105	26-27	0.2617066172156086
1105	28-29	0.22187154525580155
1105	30-31	0.2419749650351264
1105	32-33	0.24429338259737676
1105	34-35	0.21460313755434157
1105	36-37	0.20995309149952845
1105	38-39	0.15169819848602373
1105	40-41	0.1609348385425733
1105	42-43	0.28504686248120237
1105	44-45	0.2843641391029692
1105	46-47	0.291731096815937
1105	48-49	0.3195681005997315
1105	50-51	0.25955479367561196
1105	52-53	0.2614722721396774
1105	54-55	0.22056519818143983
1105	56-57	0.19156468830937712
1105	58-59	0.15860030026928484
1105	60-61	0.12380812086738757
1105	62-63	0.18231174956523688
1105	64-65	0.13086020281568267
1105	66-67	0.19891252333549403
1105	68-69	0.18400299598143022
1105	70-71	0.1639768205066332
1105	72-73	0.19099216658288043
1105	74-75	0.12481460546713663
1105	76-77	-0.023117797841344156
1105	78-79	0.20719252639695185
1105	80-81	0.15533881234225078
1105	82-83	0.18693142309928135
1105	84-85	0.16622164470172862
1105	86-87	0.14081330571868733
1105	88-89	0.16151979492516944
1105	90-91	0.18934114593387363
1105	92-93	0.1910476465567612
1105	94-95	0.22239424373798755
1105	96-97	0.20073237385076936
1105	98-99	0.23172116876319393
1105	100	0.2618328285409923
1106	1	0.42121135229991324
1106	2	0.24821136266527333
1106	3	0.19067947699389265
1106	4	0.16066791724890805
1106	5	0.16996298496204787
1106	6	0.15175778991667954
1106	7	0.14009574828185123
1106	8	0.16635720691279943
1106	9	0.19605397838373761
1106	10-11	0.1887405876256949
1106	12-13	0.23751855192257665
1106	14-15	0.2512888740459047
1106	16-17	0.2356748552621326
1106	18-19	0.22393131535979904
1106	20-21	0.28849975601062994
1106	22-23	0.31350219267650203
1106	24-25	0.264241608480404
1106	26-27	0.2508040267708225
1106	28-29	0.2566125270136155
1106	30-31	0.33192900661402547
1106	32-33	0.2744756197161422
1106	34-35	0.24652505568817418
1106	36-37	0.2915467569365404
1106	38-39	0.237101524092445
1106	40-41	0.3320374615638144
1106	42-43	0.35743697278514475
1106	44-45	0.3258196056914855
1106	46-47	0.34441993892516365
1106	48-49	0.37970787326444366
1106	50-51	0.3987842138325277
1106	52-53	0.4085030898908215
1106	54-55	0.3244380236768265
1106	56-57	0.35731349796463974
1106	58-59	0.3463936220929469
1106	60-61	0.3464296401224942
1106	62-63	0.32160498928721637
1106	64-65	0.3374493855033478
1106	66-67	0.3719317836899023
1106	68-69	0.33062230830084616
1106	70-71	0.3146191615235665
1106	72-73	0.30712240303140703
1106	74-75	0.3111643168604914
1106	76-77	-0.49079078856613023
1106	78-79	0.28968109723046354
1106	80-81	0.2902607054856574
1106	82-83	0.3202073554793543
1106	84-85	0.3395258123680449
1106	86-87	0.31764531531905504
1106	88-89	0.2662169917741082
1106	90-91	0.30547139009930646
1106	92-93	0.29709194392360416
1106	94-95	0.30963442006714104
1106	96-97	0.3667894228761881
1106	98-99	0.3863186845153379
1106	100	0.4146827665705466
1107	1	0.4924910598277279
1107	2	0.351794807689096
1107	3	0.29348065048949934
1107	4	0.2019514200129322
1107	5	0.23451772446403396
1107	6	0.21442741591529568
1107	7	0.17012104546045492
1107	8	0.22714918502018833
1107	9	0.26955878619489226
1107	10-11	0.2767725048282088
1107	12-13	0.305040983701808
1107	14-15	0.3246068853515851
1107	16-17	0.3663150473427237
1107	18-19	0.34498009053088907
1107	20-21	0.3414489523646722
1107	22-23	0.38833388428412263
1107	24-25	0.38890250635267876
1107	26-27	0.4174130036697363
1107	28-29	0.42535069615665577
1107	30-31	0.44506445666731054
1107	32-33	0.39919958689738166
1107	34-35	0.40685696836049345
1107	36-37	0.396336728302245
1107	38-39	0.4377291897337159
1107	40-41	0.48061308431985594
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2316	98-99	-0.4963817958393797
2316	100	-0.5095473525056953
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	848.0
3	244.0
4	365.0
5	641.0
6	1200.0
7	2295.0
8	4347.0
9	7148.0
10	10217.0
11	14152.0
12	18140.0
13	22429.0
14	24513.0
15	25423.0
16	30713.0
17	40274.0
18	55534.0
19	90330.0
20	192679.0
21	244595.0
22	98529.0
23	78097.0
24	91892.0
25	110494.0
26	135868.0
27	171111.0
28	219954.0
29	277441.0
30	348056.0
31	436400.0
32	551107.0
33	714924.0
34	997336.0
35	1638179.0
36	3017626.0
37	6400723.0
38	8303860.0
39	2420326.0
40	6042.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.884040826227306	35.03354915753923	18.300841527811468	30.781568488421996
2	22.01254360413758	29.726276963056957	30.795999219284877	17.465180213520586
3	23.748857075788393	27.267157219363696	26.008634813870678	22.975350890977232
4	20.844456651554026	27.30471497369129	26.378022994433824	25.472805380320857
5	23.132603234764655	30.50030644620448	25.628259488528077	20.738830830502792
6	25.493843244297544	26.255806398226657	26.125937973855596	22.124412383620207
7	22.863580476563765	26.638188882785336	28.847742871115155	21.65048776953574
8	23.59150027018303	25.641212753952274	28.146815265094993	22.6204717107697
9	23.977426994993145	25.160001928066695	28.847992833322365	22.014578243617795
10-11	21.58768569766989	28.017594130917225	28.331263123948574	22.063457047464315
12-13	21.832036812941567	26.58838298030462	27.568225505606392	24.011354701147425
14-15	22.04559780737629	29.812554086971627	25.607829741562956	22.534018364089132
16-17	24.34651671321933	26.33094988772593	25.273425077671092	24.049108321383645
18-19	22.57385002834646	27.953486286327156	25.188540523649184	24.284123161677197
20-21	24.199790762978672	27.979965491784604	25.487637093078312	22.33260665215841
22-23	23.873588217184476	26.400551304705722	27.444997868232758	22.280862609877044
24-25	22.22604923985611	27.716844290301633	25.889713352814	24.167393117028258
26-27	22.188232317417796	26.122222593334055	29.380268323807474	22.30927676544067
28-29	22.286675400485436	26.191476862978053	29.156471307572918	22.3653764289636
30-31	22.41115978363822	26.115424173734304	28.95533302532555	22.518083017301926
32-33	22.46810268572171	26.181766182440587	28.893542206253652	22.456588925584047
34-35	22.591078241162517	26.240018462476378	28.722441940154287	22.44646135620682
36-37	22.501488863991273	26.234067066052436	28.68518880750342	22.579255262452875
38-39	22.522816699504986	26.208281867234103	28.909244766425612	22.3596566668353
40-41	22.476034593575626	26.255539647512993	28.921854800162304	22.346570958749073
42-43	22.40629140698578	26.1602462194895	28.908806399868197	22.52465597365652
44-45	22.414217447421755	26.204103394516622	29.032877193343754	22.34880196471787
46-47	22.388978726052315	26.305242953565376	28.919588351790992	22.386189968591317
48-49	22.496193859047878	26.181683276841877	28.78652451502482	22.53559834908543
50-51	22.473327913257293	26.286932289192695	28.865160013866557	22.37457978368345
52-53	22.54159129205615	26.40158346331336	28.613747093999443	22.44307815063105
54-55	22.68286323464247	26.41899029264697	28.241803011711042	22.65634346099952
56-57	22.807465541424083	26.748266834837942	27.811940055839614	22.632327567898365
58-59	22.86580342098991	26.970198489171693	27.525432754306966	22.638565335531432
60-61	22.80694923923941	27.027097547640228	27.41946869065817	22.746484522462186
62-63	22.740293408765897	27.06631317573911	27.535642472161864	22.657750943333134
64-65	22.770142736711975	27.191737605729582	27.42054322535991	22.617576432198533
66-67	22.72206566108847	27.04018946988549	27.50098366670244	22.736761202323596
68-69	22.618199666229568	27.018569804296753	27.74734021557636	22.615890313897317
70-71	22.70658742193158	26.98995845851963	27.750895648165436	22.552558471383357
72-73	22.65953579892517	26.890367721183427	27.78976975515362	22.660326724737782
74-75	22.658992152788294	26.917340417637742	27.90740747580505	22.516259953768913
76-77	22.708600177316473	27.030416893684585	27.696484098747458	22.564498830251488
78-79	22.712138829119947	27.075389414654737	27.562527198684833	22.649944557540483
80-81	22.724145976722802	27.195736955565607	27.530731598942417	22.549385468769177
82-83	22.784503841620822	27.329071965420376	27.285851911673735	22.600572281285068
84-85	22.717357789918637	27.349185717847913	27.224633845529027	22.708822646704423
86-87	22.67939012739662	27.43266687451975	27.23474915328239	22.65319384480124
88-89	22.787103798916082	27.488727977660876	27.119279906068083	22.604888317354956
90-91	22.7898776958947	27.446403537059304	27.030829075156852	22.732889691889138
92-93	22.791672509376582	27.5130478586357	27.069804665608054	22.625474966379663
94-95	22.887096004870756	27.560355940966613	26.972907435280817	22.579640618881815
96-97	22.848412993677226	27.54312295767819	26.875141862879538	22.733322185765047
98-99	22.796482271218125	27.53734268755427	27.00645436767371	22.6597206735539
100	22.899265748224863	27.59375916023943	26.71350317178158	22.79347191975413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69618.0
1	42956.0
2	13016.0
3	9015.5
4	7880.5
5	7296.5
6	7100.5
7	6698.5
8	6283.0
9	6168.0
10	6521.5
11	11416.0
12	115104.0
13	125154.5
14	34308.0
15	34443.5
16	38375.0
17	42634.0
18	46787.5
19	51040.0
20	56930.0
21	66775.5
22	80552.0
23	96503.0
24	116007.5
25	139199.0
26	164738.0
27	196842.5
28	233432.0
29	271845.5
30	311011.0
31	346559.0
32	383807.0
33	426259.0
34	467259.0
35	512348.0
36	571543.0
37	620907.0
38	665410.0
39	710649.5
40	730363.0
41	755402.5
42	798690.0
43	825352.0
44	854112.5
45	899574.0
46	933103.5
47	946614.0
48	960162.5
49	965652.5
50	962943.0
51	968014.5
52	975002.0
53	970040.0
54	944007.0
55	912732.0
56	870994.0
57	802462.5
58	721826.5
59	637291.0
60	530000.5
61	423392.5
62	336362.0
63	251476.5
64	182452.0
65	134136.5
66	95128.5
67	67726.5
68	50458.5
69	39604.0
70	33164.0
71	29342.0
72	26279.5
73	21850.5
74	16830.5
75	13561.5
76	10896.5
77	8165.0
78	5548.5
79	3121.0
80	1694.5
81	1175.5
82	776.0
83	419.0
84	232.5
85	140.0
86	81.5
87	38.0
88	22.0
89	15.5
90	9.0
91	8.5
92	7.0
93	5.0
94	2.5
95	2.5
96	1.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02817484460931504
2	0.011128914389510958
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007172423035144089
26-27	0.02871580759506063
28-29	0.03348933959686394
30-31	0.030562543305019705
32-33	0.03740106160068635
34-35	0.030144696033271388
36-37	2.2011597351027375E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0057323422592972136
54-55	0.033396070116562974
56-57	0.04595573833389072
58-59	0.05091954007550799
60-61	0.055495340779073254
62-63	0.03717721484796403
64-65	0.0011043106467634072
66-67	1.3057727242134884E-5
68-69	0.0
70-71	0.0
72-73	7.83463634528093E-5
74-75	3.3577012908346843E-5
76-77	0.0
78-79	5.092513624432605E-4
80-81	0.016859391259202154
82-83	0.040792339904429376
84-85	0.055260301688714826
86-87	0.059319389471412756
88-89	0.058623599148367574
90-91	0.036623194134976306
92-93	0.0026395262925172657
94-95	1.865389606019269E-6
96-97	0.0
98-99	4.085203237182199E-4
100	1.1565415557319467E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.6804052E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.499721970546325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.72047025791011	26.74939746902101
2	15.76426076834474	12.453678348416164
3	6.011834766437365	7.123974054214206
4	3.0579846798213737	4.831581785725376
5	1.7743258039349514	3.504268797029834
6	1.150534022755351	2.726746440989435
7	0.8009318277686102	2.214560915995511
8	0.5561427100202818	1.757398593739784
9	0.4215891003843198	1.498738702589404
>10	2.393313875068705	17.37871988319703
>50	0.21864712525462318	6.008204823098837
>100	0.1235269921093384	8.483141629608589
>500	0.0057447870844771305	1.4602528515668356
>1k	6.55528189154148E-4	0.3964421472234413
>5k	9.438729214758657E-6	0.021418921475961892
>10k+	2.831618764272716E-5	3.39147463610856
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	788555	2.9419246015490494	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89192	0.3327556594801413	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31917	0.11907528011063401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8280818138988837E-4	1.865389606019269E-5	0.0	0.0	0.0
2	3.357701290834684E-4	3.3577012908346843E-5	0.0	0.0	0.0
3	4.327703885964704E-4	5.969246739261661E-5	0.0	0.0	3.730779212038538E-6
4	5.782707778659734E-4	7.461558424077076E-5	0.0	0.0	1.1192337636115613E-5
5	7.34963504771592E-4	9.700025951300199E-5	0.0	0.0	1.1192337636115613E-5
6	8.87925452465172E-4	1.268464932093103E-4	0.0	0.0	1.1192337636115613E-5
7	9.923872704022511E-4	1.492311684815415E-4	0.0	0.0	1.1192337636115613E-5
8	0.0011005798675513688	1.678850645417342E-4	0.0	3.730779212038538E-6	1.1192337636115613E-5
9	0.0012423494776088331	1.7534662296581129E-4	0.0	3.730779212038538E-6	1.1192337636115613E-5
10-11	0.0015408118145719162	1.8840435020794617E-4	0.0	3.730779212038538E-6	1.6788506454173422E-5
12-13	0.001900832008533635	2.0146207745008106E-4	0.0	9.326948030096344E-6	1.865389606019269E-5
14-15	0.00232241005949399	2.163851942982352E-4	0.0	1.3057727242134884E-5	1.865389606019269E-5
16-17	0.002861507655633559	2.4063525917648572E-4	0.0	1.4923116848154153E-5	2.4250064878250497E-5
18-19	0.0035796826539509774	2.7421227208483255E-4	0.0	1.4923116848154153E-5	2.6115454484269768E-5
20-21	0.004316511548328589	3.0592389538716013E-4	0.0	1.6788506454173422E-5	3.544240251436611E-5
22-23	0.004961936352011255	3.46962466719584E-4	0.0	2.4250064878250497E-5	4.476935054446245E-5
24-25	0.005709957584024983	3.7307792120385385E-4	0.0	2.6115454484269768E-5	5.2230908968539536E-5
26-27	0.0063385938812534764	3.9732798608210426E-4	3.730779212038538E-6	2.9846233696308306E-5	5.40962985745588E-5
28-29	0.006860902970938871	4.3650116780850895E-4	3.730779212038538E-6	2.9846233696308306E-5	5.596168818057807E-5
30-31	0.007439173748804845	4.7194357032287503E-4	3.730779212038538E-6	3.730779212038538E-5	6.528863621067441E-5
32-33	0.00793723277361199	4.8686668717102925E-4	3.730779212038538E-6	4.103857133242392E-5	7.461558424077077E-5
34-35	0.008424099460783019	5.167129208673376E-4	3.730779212038538E-6	4.103857133242392E-5	8.021175305882857E-5
36-37	0.008942677771256376	5.372322065335495E-4	3.730779212038538E-6	4.8500129756500995E-5	8.95387010889249E-5
38-39	0.009584371795727005	5.502899337756844E-4	3.730779212038538E-6	5.9692467392616605E-5	1.0446181793707907E-4
40-41	0.010211142703349479	5.596168818057807E-4	3.730779212038538E-6	6.715402581669369E-5	1.0632720754309834E-4
42-43	0.010796875039639529	5.931938947141275E-4	3.730779212038538E-6	6.715402581669369E-5	1.2311571399727176E-4
44-45	0.011416184388837926	6.081170115622818E-4	3.730779212038538E-6	6.715402581669369E-5	1.3057727242134882E-4
46-47	0.012080263088580786	6.286362972284937E-4	3.730779212038538E-6	6.715402581669369E-5	1.3430805163338737E-4
48-49	0.012751803346747723	6.584825309248019E-4	3.730779212038538E-6	6.901941542271296E-5	1.3430805163338737E-4
50-51	0.013619209513546685	6.659440893488791E-4	3.730779212038538E-6	7.461558424077076E-5	1.3617344123940662E-4
52-53	0.014704866264249897	6.715402581669368E-4	3.730779212038538E-6	7.83463634528093E-5	1.4363499966348373E-4
54-55	0.01602369671570552	7.088480502873223E-4	3.730779212038538E-6	8.207714266484784E-5	1.4550038926950298E-4
56-57	0.01763539333530617	7.442904528016884E-4	3.730779212038538E-6	8.580792187688638E-5	1.5296194769358007E-4
58-59	0.019566071577536114	7.704059072859581E-4	3.730779212038538E-6	8.580792187688638E-5	1.566927269056186E-4
60-61	0.022056366701571836	8.058483098003241E-4	3.730779212038538E-6	8.767331148290564E-5	1.6415428532969568E-4
62-63	0.025236855979834688	8.636753875869216E-4	3.730779212038538E-6	9.140409069494418E-5	1.678850645417342E-4
64-65	0.029172828048535347	8.860600628591528E-4	3.730779212038538E-6	9.326948030096345E-5	1.7534662296581129E-4
66-67	0.034353014984450855	9.084447381313839E-4	3.730779212038538E-6	9.700025951300199E-5	1.790774021778498E-4
68-69	0.040812859190095585	9.233678549795381E-4	3.730779212038538E-6	9.700025951300199E-5	1.8280818138988837E-4
70-71	0.04933768968960364	9.588102574939043E-4	3.730779212038538E-6	9.700025951300199E-5	1.8653896060192692E-4
72-73	0.060276334339300644	0.001025964283310598	7.461558424077076E-6	9.700025951300199E-5	1.977312982380425E-4
74-75	0.07408954437187332	0.0010800605818851567	7.461558424077076E-6	9.700025951300199E-5	2.051928566621196E-4
76-77	0.09174545699284571	0.001137887659671754	7.461558424077076E-6	9.886564911902127E-5	2.051928566621196E-4
78-79	0.11417676700522741	0.001165868503762043	7.461558424077076E-6	1.0073103872504053E-4	2.107890254801774E-4
80-81	0.14226020752384752	0.0011807916206101974	7.461558424077076E-6	1.0073103872504053E-4	2.163851942982352E-4
82-83	0.17798614925832856	0.0012050416854884477	7.461558424077076E-6	1.025964283310598E-4	2.163851942982352E-4
84-85	0.22308567376305644	0.001214368633518544	7.461558424077076E-6	1.1005798675513688E-4	2.2011597351027375E-4
86-87	0.2805396736284499	0.0012498110360329101	7.461558424077076E-6	1.137887659671754E-4	2.2011597351027375E-4
88	0.3307410387056405	0.0012721957113051416	7.461558424077076E-6	1.1565415557319468E-4	2.2011597351027375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	102745	0.0	79.218475	8
AGTGGTA	104970	0.0	77.90638	5
GTATCAA	104490	0.0	77.625694	9
TGGTATC	106475	0.0	76.394775	7
GTGGTAT	106785	0.0	76.37541	6
AAGCAGT	113870	0.0	72.98749	1
GCAGTGG	116550	0.0	70.89962	3
CAGTGGT	116555	0.0	70.47731	4
AGCAGTG	126515	0.0	65.842766	2
CAACGCA	99510	0.0	43.765495	12-13
ATCAACG	101130	0.0	43.031895	10-11
ACGCAGA	103235	0.0	42.409344	14-15
AGAGTAC	104925	0.0	41.296814	18-19
TCAACGC	99240	0.0	40.821144	12-13
AACGCAG	101545	0.0	39.825127	14-15
AGTACTT	109825	0.0	39.01702	20-21
GAGTACT	101210	0.0	38.87847	20-21
CGCAGAG	105525	0.0	38.287453	16-17
TATCAAC	109940	0.0	36.95077	10-11
GTACTTT	107340	0.0	36.78131	22-23
>>END_MODULE