FastQCFastQC Report
Thu 26 May 2016
SRR1660589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26312590
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18753227.127090111615771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1348640.5125455152837483No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT318710.12112452631990998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3111800.088.214198
AGTGGTA3126100.087.8691565
GTGGTAT3128450.087.720676
TGGTATC3121750.087.658127
GTATCAA3137700.087.307179
AAGCAGT3223300.086.46961
GCAGTGG3238450.085.738393
CAGTGGT3233600.085.137734
AGCAGTG3319000.083.9604952
CAACGCA3093200.045.6334412-13
ATCAACG3113300.045.328310-11
ACGCAGA3123350.045.24378614-15
AGAGTAC3039900.044.83261518-19
TCAACGC3092400.044.479112-13
AACGCAG3113000.044.15428514-15
CGCAGAG3135150.043.723116-17
AGTACTT2469850.043.49851620-21
TATCAAC3159450.043.32853310-11
GAGTACT2394750.043.2054920-21
GCAGAGT3237050.043.11607716-17
Produced by FastQC (version 0.11.5)