##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660589_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26312590
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04373013070929	34.0	31.0	34.0	30.0	34.0
2	32.18427824854946	34.0	31.0	34.0	30.0	34.0
3	32.20070544176761	34.0	31.0	34.0	30.0	34.0
4	35.57641148210799	37.0	35.0	37.0	33.0	37.0
5	35.54282269438318	37.0	35.0	37.0	33.0	37.0
6	35.579663233455925	37.0	35.0	37.0	33.0	37.0
7	35.47165930833871	37.0	35.0	37.0	33.0	37.0
8	35.533345672166824	37.0	35.0	37.0	33.0	37.0
9	37.228009291369645	39.0	38.0	39.0	34.0	39.0
10-11	37.075638202092605	39.0	37.0	39.0	34.0	39.0
12-13	37.20077825862069	39.0	37.5	39.0	34.0	39.0
14-15	38.63957392639797	41.0	39.0	41.0	35.0	41.0
16-17	38.595074639174626	41.0	39.0	41.0	34.0	41.0
18-19	38.51440703860776	40.5	38.5	41.0	34.0	41.0
20-21	38.42483347325368	40.5	38.5	41.0	34.0	41.0
22-23	38.17628490771908	40.0	38.0	41.0	33.5	41.0
24-25	38.22955374214397	40.0	38.0	41.0	34.0	41.0
26-27	38.29123083284466	40.0	38.0	41.0	34.0	41.0
28-29	38.226356413412745	40.0	38.0	41.0	34.0	41.0
30-31	38.133574079936636	40.0	38.0	41.0	33.5	41.0
32-33	38.031910940732175	40.0	38.0	41.0	33.0	41.0
34-35	37.92309601601363	40.0	38.0	41.0	33.0	41.0
36-37	37.72203756832756	40.0	38.0	41.0	33.0	41.0
38-39	37.19457641760086	40.0	35.5	41.0	32.0	41.0
40-41	37.06359626703414	40.0	35.0	41.0	32.0	41.0
42-43	36.88390453011277	40.0	35.0	41.0	31.5	41.0
44-45	36.734868061258894	40.0	35.0	41.0	31.0	41.0
46-47	36.556626428641195	39.5	35.0	41.0	31.0	41.0
48-49	36.35538599582937	39.0	35.0	41.0	30.5	41.0
50-51	35.1151046134189	38.0	34.0	40.0	28.5	40.5
52-53	34.99494789376492	38.5	34.5	39.5	28.0	40.5
54-55	35.108748511644045	39.0	35.0	40.5	25.5	41.0
56-57	34.893236792729255	39.0	35.0	41.0	22.5	41.0
58-59	34.72648169564455	39.0	35.0	41.0	22.0	41.0
60-61	34.51244168666027	39.0	35.0	41.0	22.0	41.0
62-63	34.24162258447382	38.0	35.0	41.0	20.5	41.0
64-65	33.955642755046156	37.5	35.0	40.5	20.0	41.0
66-67	33.65197652530594	37.0	34.0	40.0	19.0	41.0
68-69	33.32914770077746	36.5	34.0	39.5	18.0	41.0
70-71	33.0005428960053	36.0	34.0	39.0	16.0	41.0
72-73	32.64535585436477	35.5	34.0	39.0	12.0	41.0
74-75	32.28690362674294	35.0	34.0	37.5	7.5	40.0
76-77	31.926738549872894	35.0	33.5	37.0	4.5	39.0
78-79	31.57609112215863	35.0	33.0	37.0	2.0	39.0
80-81	31.252683696283796	35.0	33.0	36.0	2.0	39.0
82-83	30.86667534438837	35.0	33.0	36.0	2.0	37.0
84-85	30.60774758395126	35.0	33.0	35.5	2.0	37.0
86-87	30.322007202635696	35.0	32.5	35.0	2.0	36.5
88-89	30.132221970547178	35.0	32.0	35.0	2.0	36.0
90-91	29.941402613729778	35.0	32.0	35.0	2.0	36.0
92-93	29.762157678130507	35.0	32.0	35.0	2.0	36.0
94-95	29.5401892022032	35.0	32.0	35.0	2.0	35.5
96-97	29.38233334308785	35.0	32.0	35.0	2.0	35.0
98-99	29.218030133103582	35.0	32.0	35.0	2.0	35.0
100	29.092233147706096	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4121748698040406
1101	2	0.37807822564957405
1101	3	0.4315321524914211
1101	4	0.3140704439181903
1101	5	0.32214347850283787
1101	6	0.34764905112632505
1101	7	0.3356627547718247
1101	8	0.3505903291721566
1101	9	0.46625976426331306
1101	10-11	0.4817072270687177
1101	12-13	0.447948531525185
1101	14-15	0.5299682812106639
1101	16-17	0.5540316310198321
1101	18-19	0.5095496656304519
1101	20-21	0.5325619605106482
1101	22-23	0.5063751557056406
1101	24-25	0.5198060230439054
1101	26-27	0.5009555441081943
1101	28-29	0.49998216154709496
1101	30-31	0.5775916202662259
1101	32-33	0.5638502364150568
1101	34-35	0.4694240410601864
1101	36-37	0.5038919759285676
1101	38-39	0.545293412120543
1101	40-41	0.5450795360301157
1101	42-43	0.606232760122694
1101	44-45	0.5734419817057912
1101	46-47	0.5505071207973771
1101	48-49	0.5329660143737627
1101	50-51	0.435714620362468
1101	52-53	0.47853113810320025
1101	54-55	0.5543664659313592
1101	56-57	0.5411645065925512
1101	58-59	0.5303341617038839
1101	60-61	0.5116026871053663
1101	62-63	0.4801849629825554
1101	64-65	0.47092698643596975
1101	66-67	0.469014107731077
1101	68-69	0.4251155663115682
1101	70-71	0.34809208436411154
1101	72-73	0.3190254686899223
1101	74-75	0.3355440701311849
1101	76-77	0.375437954344779
1101	78-79	0.320408380055742
1101	80-81	0.32770201539991817
1101	82-83	0.27341383491784654
1101	84-85	0.2525864271086071
1101	86-87	0.18746220476311848
1101	88-89	0.1603980241862608
1101	90-91	0.03980543000663417
1101	92-93	0.0999820226151833
1101	94-95	0.10187395083433515
1101	96-97	0.045446976912202786
1101	98-99	0.08422684618123455
1101	100	0.06751612906815296
1102	1	0.34218664208452765
1102	2	0.3729748732641909
1102	3	0.3555248831186759
1102	4	0.2965110467387788
1102	5	0.2516951432629497
1102	6	0.2811356700244545
1102	7	0.3239875039499509
1102	8	0.3428045039400942
1102	9	0.41627706307593826
1102	10-11	0.334621451294808
1102	12-13	0.3972802666245414
1102	14-15	0.46833382686883596
1102	16-17	0.4886019007960698
1102	18-19	0.4409027579429363
1102	20-21	0.4643042901935033
1102	22-23	0.4536313268287131
1102	24-25	0.4799174521163181
1102	26-27	0.48451046764289885
1102	28-29	0.5003374010934323
1102	30-31	0.5109479187161909
1102	32-33	0.4623301201815764
1102	34-35	0.396711025426292
1102	36-37	0.4917561595562958
1102	38-39	0.5009472820644092
1102	40-41	0.418282178492575
1102	42-43	0.4637372494985499
1102	44-45	0.44877888778238884
1102	46-47	0.395740685848466
1102	48-49	0.4185272439277483
1102	50-51	0.4212977426612028
1102	52-53	0.4738536155069184
1102	54-55	0.5455103599357969
1102	56-57	0.594002933210696
1102	58-59	0.5320927034685994
1102	60-61	0.49430833845251243
1102	62-63	0.47401346639833974
1102	64-65	0.5162245330108561
1102	66-67	0.505224818410646
1102	68-69	0.47377890291818403
1102	70-71	0.4136425918350284
1102	72-73	0.4258371222170396
1102	74-75	0.37719292315583886
1102	76-77	0.4112296593373834
1102	78-79	0.4129938654077172
1102	80-81	0.3937441999290563
1102	82-83	0.27832611062603974
1102	84-85	0.2054210395362297
1102	86-87	0.22268480290318493
1102	88-89	0.21905166455166025
1102	90-91	0.25263322342135197
1102	92-93	0.2268671974137071
1102	94-95	0.2666533374910536
1102	96-97	0.24115840359994323
1102	98-99	0.2641750258999309
1102	100	0.2741132936768942
1103	1	0.3655644860297258
1103	2	0.36170034627087233
1103	3	0.3717992072771281
1103	4	0.3226101817153193
1103	5	0.2913055067572188
1103	6	0.30822300984681306
1103	7	0.28655027864247273
1103	8	0.31724649634993085
1103	9	0.3941508455847966
1103	10-11	0.3807895020820524
1103	12-13	0.39761909179995314
1103	14-15	0.45542270407590735
1103	16-17	0.44434781998357664
1103	18-19	0.46914298659201137
1103	20-21	0.4504645640631324
1103	22-23	0.45605617259479914
1103	24-25	0.43823392656941706
1103	26-27	0.3546535428567026
1103	28-29	0.4339796013148032
1103	30-31	0.4945102169709017
1103	32-33	0.4330445856913414
1103	34-35	0.4364635221554991
1103	36-37	0.433787302545511
1103	38-39	0.4621545384679777
1103	40-41	0.5210747963757711
1103	42-43	0.5334361159236423
1103	44-45	0.45546469394862754
1103	46-47	0.44010275348850314
1103	48-49	0.4633424431757689
1103	50-51	0.5842854078731605
1103	52-53	0.5813659461129319
1103	54-55	0.5675490490260344
1103	56-57	0.5644462736520879
1103	58-59	0.5025445219808589
1103	60-61	0.547196219094161
1103	62-63	0.5444926756680459
1103	64-65	0.5283519072830316
1103	66-67	0.5337546252703405
1103	68-69	0.5194327618839054
1103	70-71	0.4922434291927118
1103	72-73	0.43723907963745035
1103	74-75	0.4677661064580363
1103	76-77	0.5010703280906803
1103	78-79	0.4845204754489423
1103	80-81	0.4082161676851257
1103	82-83	0.35879098776960205
1103	84-85	0.32554444616522815
1103	86-87	0.32834807939462607
1103	88-89	0.29865462798948883
1103	90-91	0.2927920783259772
1103	92-93	0.3186594578532258
1103	94-95	0.29427132047417714
1103	96-97	0.29031606099990626
1103	98-99	0.27414792227065377
1103	100	0.18987242250128133
1104	1	0.26127025408290194
1104	2	0.30487363549916324
1104	3	0.2635920114703083
1104	4	0.292144616113184
1104	5	0.24067784946903004
1104	6	0.301100831308041
1104	7	0.2973882077580612
1104	8	0.31704569195219534
1104	9	0.4017198968568323
1104	10-11	0.38088558550621343
1104	12-13	0.38474408928915693
1104	14-15	0.4644770214568794
1104	16-17	0.44996564933988736
1104	18-19	0.4336886898378367
1104	20-21	0.38375037994736516
1104	22-23	0.3699745563059622
1104	24-25	0.4393675639524375
1104	26-27	0.41884904438521176
1104	28-29	0.46616840521099334
1104	30-31	0.47568339406502247
1104	32-33	0.450029643654716
1104	34-35	0.44562918643926963
1104	36-37	0.4394021566907327
1104	38-39	0.4186186966710892
1104	40-41	0.43259440993092113
1104	42-43	0.37152558744529074
1104	44-45	0.41228698759975657
1104	46-47	0.38640537868960223
1104	48-49	0.39595702113795284
1104	50-51	0.4001345630214814
1104	52-53	0.47656088432817967
1104	54-55	0.4711288672244436
1104	56-57	0.39676949798302275
1104	58-59	0.38511280337355913
1104	60-61	0.3720595709763188
1104	62-63	0.3147189181703496
1104	64-65	0.3137306987425461
1104	66-67	0.31654408902166864
1104	68-69	0.3336062448635033
1104	70-71	0.26605919434656755
1104	72-73	0.2722491381357628
1104	74-75	0.2772302052761333
1104	76-77	0.32260935839993365
1104	78-79	0.3157989437240438
1104	80-81	0.32031070477436785
1104	82-83	0.20791165792105204
1104	84-85	0.20500928436550225
1104	86-87	0.17443985799131312
1104	88-89	0.18966420165256537
1104	90-91	0.13068111052169584
1104	92-93	0.13973202075847624
1104	94-95	0.1509812450214305
1104	96-97	0.14881140773994872
1104	98-99	0.09765991986155598
1104	100	0.0877918817189638
1105	1	0.2398460013090542
1105	2	0.24316766492308517
1105	3	0.171422692103107
1105	4	0.23624484631141485
1105	5	0.20474600647645502
1105	6	0.18450486872671235
1105	7	0.21783122718863268
1105	8	0.14800907084136838
1105	9	0.2653332075743151
1105	10-11	0.29211109930078294
1105	12-13	0.2697752603366439
1105	14-15	0.3370871200651351
1105	16-17	0.3561058746649479
1105	18-19	0.366302812963319
1105	20-21	0.26912966973863917
1105	22-23	0.36550166507394266
1105	24-25	0.36808420121562335
1105	26-27	0.36687790514745444
1105	28-29	0.3523311553781312
1105	30-31	0.3095941613930009
1105	32-33	0.2861279616284591
1105	34-35	0.3358558551369626
1105	36-37	0.3428029463934621
1105	38-39	0.319278880360244
1105	40-41	0.3109100436913934
1105	42-43	0.331713194135709
1105	44-45	0.34289384344188534
1105	46-47	0.30171331373464483
1105	48-49	0.30938509388997915
1105	50-51	0.4166499937438246
1105	52-53	0.41530473917539723
1105	54-55	0.3921830392540713
1105	56-57	0.3517623111740491
1105	58-59	0.26917486492592246
1105	60-61	0.28345576389958893
1105	62-63	0.2527198747616026
1105	64-65	0.2553586985886227
1105	66-67	0.2476218309425704
1105	68-69	0.2169896966490512
1105	70-71	0.26717419718153224
1105	72-73	0.20036187267905348
1105	74-75	0.20729291461530153
1105	76-77	0.18203949761901939
1105	78-79	0.18515541296183713
1105	80-81	0.15024963366359856
1105	82-83	0.12668799950795773
1105	84-85	0.10643978108880603
1105	86-87	0.05409784667795847
1105	88-89	0.0709684070934351
1105	90-91	0.07044124258909079
1105	92-93	0.04331650280765942
1105	94-95	0.06448303326109439
1105	96-97	0.0035201208317090504
1105	98-99	0.021733858569781717
1105	100	0.02680270951449515
1106	1	0.2398168475791067
1106	2	0.2491600185687659
1106	3	0.2573228227725508
1106	4	0.18739573281793298
1106	5	0.16077454583826523
1106	6	0.22356343267230727
1106	7	0.2212396640987606
1106	8	0.2421379197862592
1106	9	0.27948769642266313
1106	10-11	0.2872631548242879
1106	12-13	0.28186791694744073
1106	14-15	0.3078981730663628
1106	16-17	0.29239011265957515
1106	18-19	0.30405290198090285
1106	20-21	0.386066355194167
1106	22-23	0.42388675847653445
1106	24-25	0.37686621559229394
1106	26-27	0.3895976297012709
1106	28-29	0.4267294864523592
1106	30-31	0.3699878698945369
1106	32-33	0.327915408924504
1106	34-35	0.397279492970803
1106	36-37	0.38343286282071887
1106	38-39	0.3863479374111165
1106	40-41	0.41288649546012834
1106	42-43	0.47063759451323506
1106	44-45	0.44407871440191826
1106	46-47	0.4369354653695723
1106	48-49	0.3797289828710291
1106	50-51	0.4155494388625627
1106	52-53	0.39545038413214684
1106	54-55	0.446722958627511
1106	56-57	0.4567853907506745
1106	58-59	0.45019671166942743
1106	60-61	0.4533785262903507
1106	62-63	0.4208623884735019
1106	64-65	0.4179812114780077
1106	66-67	0.3702205735047954
1106	68-69	0.4278471794311969
1106	70-71	0.4127583978200846
1106	72-73	0.2969273058206099
1106	74-75	0.33689780649205403
1106	76-77	0.3398420718593407
1106	78-79	0.34348063186396516
1106	80-81	0.32199959449743076
1106	82-83	0.3155806458451451
1106	84-85	0.29014834368913967
1106	86-87	0.26528769647802974
1106	88-89	0.25166748602575595
1106	90-91	0.27401712357373853
1106	92-93	0.2713062958507635
1106	94-95	0.31076960999874714
1106	96-97	0.1962137607387895
1106	98-99	0.15044078263185412
1106	100	0.13121259564169918
1107	1	0.26938017630113364
1107	2	0.282377703320158
1107	3	0.33726721063440834
1107	4	0.29127746730085136
1107	5	0.2711116963095819
1107	6	0.3346753392857522
1107	7	0.3260310267265041
1107	8	0.3464889536826803
1107	9	0.42867978050769295
1107	10-11	0.40528534866383836
1107	12-13	0.3761397925776464
1107	14-15	0.4704273499983529
1107	16-17	0.4339721343314409
1107	18-19	0.43468454019875935
1107	20-21	0.47105057293693875
1107	22-23	0.5355249011443419
1107	24-25	0.5060375912868054
1107	26-27	0.5239495944393582
1107	28-29	0.510875470315753
1107	30-31	0.5162334317125712
1107	32-33	0.4847988817263129
1107	34-35	0.514053821100724
1107	36-37	0.5343383001425934
1107	38-39	0.5338645671222935
1107	40-41	0.5941137878972214
1107	42-43	0.5686022603108896
1107	44-45	0.5801523629204226
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2309	100	0.17913323581770513
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2310	22-23	-0.1349760770265931
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2310	100	0.20415239206134927
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2311	2	-0.14753504502940018
2311	3	-0.15874660031409604
2311	4	-0.12863906654496304
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2311	10-11	-0.17266222773312734
2311	12-13	-0.19307304574506645
2311	14-15	-0.26951579642699386
2311	16-17	-0.20803638596186147
2311	18-19	-0.24122634066895188
2311	20-21	-0.25476301793560907
2311	22-23	-0.285392029182006
2311	24-25	-0.2973359497615249
2311	26-27	-0.3498244260310699
2311	28-29	-0.2700255229898687
2311	30-31	-0.18954993076095406
2311	32-33	-0.16277607410030726
2311	34-35	-0.1343528547097108
2311	36-37	-0.18003831664922387
2311	38-39	-0.1522747298038425
2311	40-41	-0.20117481043850916
2311	42-43	-0.18550125022748176
2311	44-45	-0.21202978339297118
2311	46-47	-0.284498067075063
2311	48-49	-0.21651094411412686
2311	50-51	-0.2697314781435054
2311	52-53	-0.2766531178401266
2311	54-55	-0.28153325230617554
2311	56-57	-0.27533892897370293
2311	58-59	-0.32573017030781415
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2311	62-63	-0.27342694598024764
2311	64-65	-0.29122274168926765
2311	66-67	-0.28524911733017433
2311	68-69	-0.2233443826623116
2311	70-71	-0.24646108595031535
2311	72-73	-0.24690355452506907
2311	74-75	-0.26199183275696214
2311	76-77	-0.23433275765981065
2311	78-79	-0.2544590261443531
2311	80-81	-0.23695846522471342
2311	82-83	-0.16865739078929565
2311	84-85	-0.18911305872181927
2311	86-87	-0.07068187637896273
2311	88-89	-0.07620376218617864
2311	90-91	-0.10633072368924346
2311	92-93	-0.08703697687485956
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2311	96-97	-0.10205405702827974
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2312	3	-0.273073889642621
2312	4	-0.22707657861064945
2312	5	-0.2944030030109417
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2312	10-11	-0.29979703797488355
2312	12-13	-0.23310010409798565
2312	14-15	-0.26448026672102287
2312	16-17	-0.2933634316246696
2312	18-19	-0.39254627904660566
2312	20-21	-0.35168791073875383
2312	22-23	-0.33266426010284533
2312	24-25	-0.286888571796716
2312	26-27	-0.3336059096093038
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2312	36-37	-0.35603526013301234
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2312	40-41	-0.28159427083089383
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2312	44-45	-0.3167463905000716
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2312	48-49	-0.33782066525115084
2312	50-51	-0.2818852849494533
2312	52-53	-0.3564195406580808
2312	54-55	-0.42053285998546386
2312	56-57	-0.3872125817023644
2312	58-59	-0.38795056206954115
2312	60-61	-0.3517718894378348
2312	62-63	-0.27344312809942295
2312	64-65	-0.31411463855003774
2312	66-67	-0.2908555804011499
2312	68-69	-0.3362231217563192
2312	70-71	-0.3389052845751763
2312	72-73	-0.28405190635778155
2312	74-75	-0.27878262194209924
2312	76-77	-0.27929218243103193
2312	78-79	-0.24518642242429323
2312	80-81	-0.22071917104392114
2312	82-83	-0.22554923325408538
2312	84-85	-0.18122124489008584
2312	86-87	-0.1003829602460975
2312	88-89	-0.11467313894931053
2312	90-91	-0.1296816355632764
2312	92-93	-0.12835776422151568
2312	94-95	-0.09932691383326997
2312	96-97	-0.09396421041285308
2312	98-99	-0.08632637749283134
2312	100	-0.10703951292211045
2313	1	-0.3196843701198553
2313	2	-0.31149114991871585
2313	3	-0.35221493397691717
2313	4	-0.335226630065641
2313	5	-0.2967831657313482
2313	6	-0.3031751125980975
2313	7	-0.30285925604070485
2313	8	-0.3312187835385174
2313	9	-0.3777643097950829
2313	10-11	-0.34815038909673746
2313	12-13	-0.4251412191391353
2313	14-15	-0.4138097544871968
2313	16-17	-0.36940800209899294
2313	18-19	-0.38885052875562565
2313	20-21	-0.4022409260523716
2313	22-23	-0.40111759112512857
2313	24-25	-0.37954674409283484
2313	26-27	-0.3898943116607825
2313	28-29	-0.3615173801324545
2313	30-31	-0.386723436106557
2313	32-33	-0.4195231386875875
2313	34-35	-0.44804445187156716
2313	36-37	-0.3942631760391393
2313	38-39	-0.366405517291291
2313	40-41	-0.45042487587268454
2313	42-43	-0.4014564203698825
2313	44-45	-0.41967774438420946
2313	46-47	-0.3009589007125584
2313	48-49	-0.36978222070660394
2313	50-51	-0.23968738379157628
2313	52-53	-0.34628011890286103
2313	54-55	-0.4583482336204341
2313	56-57	-0.4264094121822737
2313	58-59	-0.4070781995003401
2313	60-61	-0.3798780532228676
2313	62-63	-0.3594840449175365
2313	64-65	-0.3969491512043106
2313	66-67	-0.34041486978278357
2313	68-69	-0.3339319268181953
2313	70-71	-0.3503610699247872
2313	72-73	-0.4103378088222911
2313	74-75	-0.3551697268024938
2313	76-77	-0.2766904484478623
2313	78-79	-0.254135619578701
2313	80-81	-0.2202577359797253
2313	82-83	-0.2348564710016312
2313	84-85	-0.26875415474828657
2313	86-87	-0.15902339497801776
2313	88-89	-0.13630672685276934
2313	90-91	-0.18226216894909442
2313	92-93	-0.21829556199444866
2313	94-95	-0.13907639565297458
2313	96-97	-0.15510750140084895
2313	98-99	-0.10929825523066938
2313	100	-0.047636344471985836
2314	1	-0.4207538155870978
2314	2	-0.4444711959934544
2314	3	-0.43350080108642075
2314	4	-0.4418158947950843
2314	5	-0.34690101807646556
2314	6	-0.38019597637359226
2314	7	-0.43518522395727643
2314	8	-0.42670082413546595
2314	9	-0.525776723349523
2314	10-11	-0.45414233524456904
2314	12-13	-0.47229166116714794
2314	14-15	-0.5306147020310235
2314	16-17	-0.5497845186384467
2314	18-19	-0.5394813279567998
2314	20-21	-0.5153925347515198
2314	22-23	-0.5432278441121312
2314	24-25	-0.5882217634711111
2314	26-27	-0.5586279294677254
2314	28-29	-0.5124122906622048
2314	30-31	-0.5200451996474627
2314	32-33	-0.5519154042480352
2314	34-35	-0.5840851241498299
2314	36-37	-0.5130154961249787
2314	38-39	-0.5075247311016469
2314	40-41	-0.6217961237983474
2314	42-43	-0.5692830612157778
2314	44-45	-0.5559061757306907
2314	46-47	-0.5926260010270639
2314	48-49	-0.5432778984974149
2314	50-51	-0.5014395075016793
2314	52-53	-0.5717519522469701
2314	54-55	-0.5752186507562058
2314	56-57	-0.624872414409829
2314	58-59	-0.5284863657982868
2314	60-61	-0.5613546789518011
2314	62-63	-0.516681256503098
2314	64-65	-0.49458837665236643
2314	66-67	-0.48584471840887744
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2314	70-71	-0.4846775996625894
2314	72-73	-0.4715521316377007
2314	74-75	-0.4241508215863661
2314	76-77	-0.4347631904249276
2314	78-79	-0.4562103472400061
2314	80-81	-0.40832039194879854
2314	82-83	-0.38927089009004945
2314	84-85	-0.440657307251648
2314	86-87	-0.34925332555052435
2314	88-89	-0.35274905467647955
2314	90-91	-0.36500578543418527
2314	92-93	-0.31514979737004367
2314	94-95	-0.2905592775842294
2314	96-97	-0.3046964812332398
2314	98-99	-0.3062186281985504
2314	100	-0.3014304366725895
2315	1	-0.4739024810853891
2315	2	-0.46031911757417276
2315	3	-0.4864796217904228
2315	4	-0.4539329941185031
2315	5	-0.3884554722049103
2315	6	-0.42677660752787716
2315	7	-0.4260722507181214
2315	8	-0.4564996875933929
2315	9	-0.4917859403035365
2315	10-11	-0.5759976946217265
2315	12-13	-0.5499499120933606
2315	14-15	-0.5951465046607396
2315	16-17	-0.6729245712424756
2315	18-19	-0.6818353503669954
2315	20-21	-0.6961371562717247
2315	22-23	-0.7076759640922106
2315	24-25	-0.7095740900177958
2315	26-27	-0.6900864122183208
2315	28-29	-0.6705301289849928
2315	30-31	-0.6631118063419663
2315	32-33	-0.6736489193377722
2315	34-35	-0.7216596734479666
2315	36-37	-0.7095529638167122
2315	38-39	-0.7114481011704541
2315	40-41	-0.751880616762044
2315	42-43	-0.8396997695395569
2315	44-45	-0.799435709249444
2315	46-47	-0.7065198300789888
2315	48-49	-0.7126998884673625
2315	50-51	-0.6834997744340185
2315	52-53	-0.8272019926756684
2315	54-55	-0.7424970770050976
2315	56-57	-0.7837771142684673
2315	58-59	-0.7544696343183475
2315	60-61	-0.7346143146208561
2315	62-63	-0.7119229641360292
2315	64-65	-0.7562288531080128
2315	66-67	-0.6701541264030837
2315	68-69	-0.6540769569106715
2315	70-71	-0.7336554151320058
2315	72-73	-0.6569795355967116
2315	74-75	-0.6033288697442387
2315	76-77	-0.6037554046732367
2315	78-79	-0.57489959304068
2315	80-81	-0.5723926515947788
2315	82-83	-0.5118603607609202
2315	84-85	-0.4719614135636867
2315	86-87	-0.4908150957059263
2315	88-89	-0.5072358197938236
2315	90-91	-0.555008407493002
2315	92-93	-0.6265324845319391
2315	94-95	-0.45222579837031773
2315	96-97	-0.42812053111469694
2315	98-99	-0.35075491744766296
2315	100	-0.3384609529336018
2316	1	-0.6552388182334639
2316	2	-0.5924269397101902
2316	3	-0.63629501795862
2316	4	-0.5819987190454228
2316	5	-0.5762252308217768
2316	6	-0.5679229158573023
2316	7	-0.5758184863611646
2316	8	-0.5662585153015343
2316	9	-0.6445290548666733
2316	10-11	-0.6621766686576223
2316	12-13	-0.6647850834582982
2316	14-15	-0.8713979836660641
2316	16-17	-0.9767768469154845
2316	18-19	-0.9368478196404837
2316	20-21	-0.8537554070365303
2316	22-23	-0.8922518984507306
2316	24-25	-0.8239042931881926
2316	26-27	-0.8568526604491211
2316	28-29	-0.823554278243769
2316	30-31	-0.792686200824825
2316	32-33	-0.8823461698435864
2316	34-35	-0.89057362343965
2316	36-37	-0.8878263011018674
2316	38-39	-0.8294948144059546
2316	40-41	-1.0065162499188887
2316	42-43	-0.9190894432171959
2316	44-45	-0.8791326386531253
2316	46-47	-0.8634225056906573
2316	48-49	-0.9419571059096725
2316	50-51	-0.8477478365852846
2316	52-53	-0.9422365544479234
2316	54-55	-0.983775953263212
2316	56-57	-0.9099937029098513
2316	58-59	-0.9477859412453284
2316	60-61	-1.3377040895131955
2316	62-63	-1.2540589521477372
2316	64-65	-0.9607005668238457
2316	66-67	-0.8551941974195856
2316	68-69	-0.7730274673865694
2316	70-71	-0.7113485321449815
2316	72-73	-0.7671670779385167
2316	74-75	-0.718232550766686
2316	76-77	-0.7016056554716279
2316	78-79	-0.6877926977259676
2316	80-81	-0.6850082658176468
2316	82-83	-0.6552090188557429
2316	84-85	-0.6402044637132711
2316	86-87	-0.6377855468857696
2316	88-89	-0.6026406567947689
2316	90-91	-0.6651757541532071
2316	92-93	-0.6612136955633581
2316	94-95	-0.6028789779601702
2316	96-97	-0.6780320313928918
2316	98-99	-0.6419045668299361
2316	100	-0.5595079909307294
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	223123.0
3	41664.0
4	26409.0
5	25658.0
6	26990.0
7	28814.0
8	35296.0
9	45063.0
10	53402.0
11	59461.0
12	56980.0
13	56478.0
14	53312.0
15	52091.0
16	69717.0
17	100953.0
18	145622.0
19	233679.0
20	439583.0
21	482967.0
22	197170.0
23	150595.0
24	167064.0
25	182909.0
26	193586.0
27	214726.0
28	251465.0
29	300553.0
30	358610.0
31	439248.0
32	552521.0
33	718002.0
34	993759.0
35	1630081.0
36	2931168.0
37	5438371.0
38	7051551.0
39	2277659.0
40	6290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.285757055967713	40.98188717780602	20.348464503372302	25.383891262853965
2	20.105053448876234	37.097020520130116	27.33284539698359	15.46508063401006
3	31.016562339861153	25.874425591611548	22.915862090575025	20.19314997795227
4	19.03606768742251	25.54213795502271	23.54708976506683	31.87470459248795
5	20.598278668820367	36.91026695641573	23.444877433450117	19.046576941313784
6	31.238844434349357	24.753973086820373	23.93883136526641	20.06835111356386
7	20.368242573995584	24.9556297801059	35.198824475512076	19.47730317038644
8	29.846676838552817	24.34915388072859	25.768207741547055	20.035961539171538
9	30.066699661620948	23.83606112553134	26.452169018651283	19.645070194196425
10-11	19.30096320644466	30.489065224743538	30.540697577730047	19.669273991081752
12-13	19.434948920347953	25.023232640576097	29.89605435278142	25.64576408629453
14-15	19.674623072878962	36.257109910578684	23.881714015830195	20.186553000712156
16-17	25.964247935009976	24.662456636403494	23.629954487549607	25.743340941036923
18-19	20.28951511132198	30.34724348341051	23.509416980301502	25.85382442496601
20-21	25.976147234664616	30.35204565087149	23.682080410359386	19.98972670410451
22-23	25.614375265613305	24.769178481995926	29.616437497346126	20.000008755044643
24-25	19.974748171630978	29.990455387453217	24.187271959412843	25.84752448150296
26-27	20.017454301256468	25.61744224359573	34.26799741932567	20.097106035822137
28-29	22.436912333136473	24.588039813738835	32.88613442150386	20.088913431620835
30-31	21.95071970076682	24.784088437680445	32.86359212091211	20.401599740640624
32-33	21.01784892283344	25.23708679918447	33.022141118985246	20.722923158996835
34-35	20.884733466021068	25.129684907729043	33.09668263912802	20.88889898712186
36-37	20.919628310216194	25.085710965634977	33.079963324379094	20.91469739976973
38-39	21.04251519736088	25.125746375729225	33.11878966971289	20.712948757197005
40-41	20.892311567202096	25.28313662525774	33.095844160529744	20.72870764701042
42-43	20.895482311900423	25.196647614109295	33.08636034256734	20.82150973142294
44-45	20.89526340168985	25.228187362272926	33.061934314882855	20.814614921154377
46-47	20.929871605224335	25.27284451217818	32.883110232950884	20.9141736496466
48-49	20.97674184681709	25.331321545285984	32.687859719114506	21.004076888782418
50-51	21.08394324604823	25.40971095510264	32.607988627424795	20.898357171424344
52-53	21.26301420723452	25.64440259717174	32.09246150336607	21.00012169222768
54-55	21.53224682292239	26.00790520905444	31.146382451573295	21.31346551644988
56-57	21.887877431092846	26.75287409807429	29.94014992626555	21.41909854456731
58-59	21.98915827420005	27.288754012264505	29.291243681147293	21.43084403238815
60-61	21.95242280976632	27.445440526723253	29.039803170763417	21.562333492747012
62-63	21.76704404739028	27.553226865330004	29.125471468996516	21.554257618283202
64-65	21.74685500357947	27.53853687133731	29.17442769949352	21.540180425589703
66-67	21.664858722975413	27.20973558407176	29.545211835837637	21.580193857115194
68-69	21.530417509225934	27.041537312265945	30.046896069136054	21.381149109372068
70-71	21.542985527473054	26.901014344872003	30.23260469727177	21.323395430383172
72-73	21.483653663585702	26.8171562193062	30.315726307262448	21.383463809845647
74-75	21.563935526462004	26.882434721200294	30.23734833628822	21.316281416049485
76-77	21.682172655184733	27.08241911113204	29.844080229924092	21.391328003759146
78-79	21.746022842305308	27.282880466740306	29.456661402572998	21.514435288381392
80-81	21.818057665950036	27.517231993640944	29.164246146032774	21.500464194376246
82-83	21.937075900928217	27.64522546323167	28.813234118105775	21.604464517734336
84-85	21.876375444742976	27.73868253267684	28.63409230760807	21.75084971497211
86-87	21.94695954985782	27.77515888880121	28.564421226117897	21.71346033522308
88-89	22.067420631973302	27.875002283597944	28.39127126267311	21.666305821755643
90-91	22.10700884006232	27.915940283887643	28.217828199837065	21.759222676212982
92-93	22.203349663602744	27.938989678903187	28.196889544776827	21.66077111271724
94-95	22.26450535779556	28.01121170658767	28.05123773226696	21.67304520334981
96-97	22.245104148501703	27.92193031421983	28.029391952865197	21.80357358441327
98-99	22.354950526006807	27.921092813217708	27.95557500202934	21.76838165874614
100	22.53789839199973	27.93495057895124	27.792614176505907	21.734536852543123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94277.0
1	56453.0
2	15326.5
3	11480.5
4	10739.0
5	10302.5
6	10119.0
7	10119.0
8	10082.5
9	10164.0
10	10844.5
11	20372.5
12	250766.0
13	278397.5
14	79938.5
15	79188.5
16	88290.0
17	97808.5
18	104795.0
19	111502.5
20	120101.0
21	131943.0
22	148729.5
23	167599.5
24	187924.0
25	210592.0
26	235174.5
27	266778.0
28	306974.0
29	343995.5
30	373372.0
31	402751.0
32	434016.5
33	462780.5
34	492886.0
35	527529.5
36	565596.5
37	599463.5
38	633966.5
39	668735.5
40	690601.0
41	720900.0
42	758498.0
43	778994.5
44	797703.0
45	826924.5
46	854890.5
47	875327.0
48	883460.5
49	880746.5
50	875939.0
51	875101.0
52	871945.5
53	853162.5
54	819617.5
55	785117.5
56	744739.5
57	691690.5
58	623484.5
59	538411.5
60	445089.5
61	358538.0
62	286504.0
63	218373.0
64	161391.0
65	120515.0
66	87661.0
67	63917.5
68	48331.0
69	37548.5
70	30301.5
71	25021.5
72	20779.5
73	16639.0
74	13012.5
75	10758.0
76	8535.0
77	5855.5
78	3780.0
79	2489.5
80	1603.0
81	1128.5
82	774.5
83	537.5
84	376.5
85	249.5
86	183.5
87	134.0
88	108.0
89	96.0
90	78.0
91	66.0
92	52.0
93	40.0
94	35.0
95	27.5
96	22.5
97	19.0
98	13.5
99	9.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02250633632036983
2	0.03792481089850904
3	0.05737937618455652
4	0.13427032458606317
5	0.17901696488259045
6	0.11091648522627381
7	0.12380385207233495
8	0.15019806108026615
9	0.13622756254705448
10-11	0.1347681851159464
12-13	0.13470167702989327
14-15	0.14775056351351196
16-17	0.13823610674585815
18-19	0.1414094925661062
20-21	0.1505724065931936
22-23	0.1597011164617394
24-25	0.15667404843080823
26-27	0.1475985450311049
28-29	0.16595857724382132
30-31	0.15850397091278356
32-33	0.1547719171696895
34-35	0.1420080653405841
36-37	0.15031397517310155
38-39	0.14583703086621272
40-41	0.14037386665470789
42-43	0.14437765343510464
44-45	0.13762043189210943
46-47	0.14619617453089948
48-49	0.13983230081113263
50-51	0.1423292043846691
52-53	0.1573277279051587
54-55	0.15986263609929696
56-57	0.15433296380173903
58-59	0.15209449164829458
60-61	0.15305790878054953
62-63	0.14667123228842163
64-65	0.14606885905188355
66-67	0.1594388845795872
68-69	0.1537362912582912
70-71	0.14683655238803933
72-73	0.1500061377462272
74-75	0.14994533035326435
76-77	0.11402336296046875
78-79	0.03148112747547847
80-81	0.04016898374504372
82-83	0.1704431224748305
84-85	0.04562834749448838
86-87	0.18748629458369548
88-89	0.041400333452541155
90-91	0.17817136207420098
92-93	0.187347577718499
94-95	0.2457682805075441
96-97	0.2721054825845727
98-99	0.2993110902423517
100	0.3161186337034857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.631259E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.67407283639205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3436956355581	30.445260639691924
2	13.92446365780818	11.884271526820008
3	5.345687446936169	6.843667664134223
4	2.7892568234740898	4.761157953773474
5	1.5954575452605881	3.4042335746910806
6	1.0924611675481326	2.7971860460927354
7	0.6917284787918956	2.0663210040880418
8	0.5304502295424508	1.8109177385260344
9	0.3968001892454524	1.5239772159619127
>10	2.0618905845482227	15.85360581668945
>50	0.1575327929123686	4.666550759697991
>100	0.06840211737896053	4.724497040018922
>500	0.0012024479173639506	0.3374811809488532
>1k	8.286101821276933E-4	0.7497711439478213
>5k	9.7812615930968E-5	0.29247277854340303
>10k+	4.446027996754917E-5	7.838627916374224
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1875322	7.127090111615771	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	134864	0.5125455152837483	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31871	0.12112452631990998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.881201356460918E-5	1.140138618053183E-5	0.0	0.0	0.0
2	1.3301617210620468E-4	3.040369648141821E-5	0.0	3.8004620601772763E-6	0.0
3	1.8622264094868653E-4	3.420415854159549E-5	0.0	3.8004620601772763E-6	0.0
4	2.3182818567081385E-4	6.080739296283642E-5	0.0	3.8004620601772763E-6	1.5201848240709105E-5
5	3.116378889345367E-4	6.840831708319098E-5	0.0	3.8004620601772763E-6	1.5201848240709105E-5
6	3.686448198371958E-4	7.98097032637228E-5	0.0	3.8004620601772763E-6	1.900231030088638E-5
7	4.2185128867967765E-4	8.361016532390009E-5	0.0	3.8004620601772763E-6	2.280277236106366E-5
8	5.130623781239323E-4	9.121108944425463E-5	0.0	3.8004620601772763E-6	2.280277236106366E-5
9	5.966725434478324E-4	1.0261247562478646E-4	0.0	3.8004620601772763E-6	2.280277236106366E-5
10-11	8.247002670584689E-4	1.1211363077522966E-4	0.0	3.8004620601772763E-6	2.280277236106366E-5
12-13	0.0011268370008425625	1.235150169557615E-4	0.0	3.8004620601772763E-6	2.280277236106366E-5
14-15	0.0014517765069877195	1.3111594107611602E-4	0.0	3.8004620601772763E-6	2.47030033911523E-5
16-17	0.001839423637125802	1.5391871343717968E-4	0.0	5.700693090265915E-6	3.420415854159549E-5
18-19	0.002354386246279823	1.7292102373806607E-4	0.0	1.140138618053183E-5	3.610438957168412E-5
20-21	0.002799040307320564	1.8622264094868653E-4	0.0	1.140138618053183E-5	4.560554472212732E-5
22-23	0.003239893906301128	1.9002310300886383E-4	0.0	1.140138618053183E-5	4.940600678230459E-5
24-25	0.003638942422619742	1.976240271292184E-4	0.0	1.3301617210620466E-5	5.510669987257051E-5
26-27	0.004056993249239242	2.185265684601934E-4	0.0	1.5201848240709105E-5	5.7006930902659146E-5
28-29	0.004402835296715375	2.394291097911684E-4	0.0	1.5201848240709105E-5	5.7006930902659146E-5
30-31	0.004649865330626898	2.6603234421240937E-4	0.0	1.900231030088638E-5	6.46078550230137E-5
32-33	0.004915897674839307	3.0213673378409343E-4	0.0	1.900231030088638E-5	7.220877914336824E-5
34-35	0.005185730481111894	3.135381199646253E-4	0.0	1.900231030088638E-5	9.50115515044319E-5
36-37	0.005504969294166785	3.2683973717524575E-4	0.0	2.0902541330975018E-5	1.045127066548751E-4
38-39	0.005793804410740257	3.382411233557776E-4	0.0	2.6603234421240936E-5	1.1211363077522966E-4
40-41	0.006137746227186301	3.382411233557776E-4	0.0	2.6603234421240936E-5	1.1401386180531829E-4
42-43	0.006487388736722611	3.4584204747613214E-4	0.0	2.6603234421240936E-5	1.1781432386549556E-4
44-45	0.006956745801154504	3.4584204747613214E-4	0.0	2.6603234421240936E-5	1.2161478592567284E-4
46-47	0.007502112106789943	3.686448198371958E-4	0.0	2.6603234421240936E-5	1.2541524798585012E-4
48-49	0.008332513066938678	3.9144759219825946E-4	0.0	3.040369648141821E-5	1.3871686519647058E-4
50-51	0.009326333895675037	3.971482852885254E-4	0.0	3.420415854159549E-5	1.4251732725664788E-4
52-53	0.010618490996135311	4.1044990249914585E-4	0.0	3.99048516318614E-5	1.501182513770024E-4
54-55	0.01219568275110888	4.25651750739855E-4	0.0	4.370531369203868E-5	1.596194065274456E-4
56-57	0.014213728105063014	4.3515290589029817E-4	0.0	5.320646884248187E-5	1.596194065274456E-4
58-59	0.016761937916411878	4.5985590928145043E-4	0.0	5.510669987257051E-5	1.6722033064780017E-4
60-61	0.019878316805757246	4.7125729546198224E-4	0.0	6.46078550230137E-5	1.7102079270797744E-4
62-63	0.024022720682380566	5.016609919434005E-4	0.0	6.46078550230137E-5	1.748212547681547E-4
64-65	0.029461181890494244	5.263639953345528E-4	0.0	6.840831708319098E-5	1.8242217888850927E-4
66-67	0.03635141960559565	5.453663056354391E-4	0.0	7.410901017345689E-5	1.938235650690411E-4
68-69	0.04579556782513618	5.548674607858824E-4	0.0	7.600924120354552E-5	2.0142448918939566E-4
70-71	0.05808436189671941	5.795704641770347E-4	0.0	7.98097032637228E-5	2.1092564433983885E-4
72-73	0.07368525865374712	6.061736985982756E-4	0.0	7.98097032637228E-5	2.2612749258054795E-4
74-75	0.09361108123525658	6.346771640496052E-4	0.0	7.98097032637228E-5	2.394291097911684E-4
76-77	0.1187796412287806	6.536794743504915E-4	0.0	8.170993429381144E-5	2.4322957185134568E-4
78-79	0.15047549481065908	6.764822467115552E-4	0.0	8.361016532390009E-5	2.489302649416116E-4
80-81	0.1906121746281913	6.878836328920871E-4	0.0	8.361016532390009E-5	2.565311890619661E-4
82-83	0.2409492946152393	6.992850190726188E-4	0.0	8.361016532390009E-5	2.6033165112214344E-4
84-85	0.3049832798671662	7.14486867313328E-4	0.0	8.551039635398872E-5	2.6223188215223204E-4
86-87	0.3852623401953209	7.277884845239484E-4	0.0	8.741062738407735E-5	2.6983280627258663E-4
88	0.4567357299300449	7.486910258549235E-4	0.0	9.121108944425463E-5	2.6983280627258663E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	311180	0.0	88.21419	8
AGTGGTA	312610	0.0	87.869156	5
GTGGTAT	312845	0.0	87.72067	6
TGGTATC	312175	0.0	87.65812	7
GTATCAA	313770	0.0	87.30717	9
AAGCAGT	322330	0.0	86.4696	1
GCAGTGG	323845	0.0	85.73839	3
CAGTGGT	323360	0.0	85.13773	4
AGCAGTG	331900	0.0	83.960495	2
CAACGCA	309320	0.0	45.63344	12-13
ATCAACG	311330	0.0	45.3283	10-11
ACGCAGA	312335	0.0	45.243786	14-15
AGAGTAC	303990	0.0	44.832615	18-19
TCAACGC	309240	0.0	44.4791	12-13
AACGCAG	311300	0.0	44.154285	14-15
CGCAGAG	313515	0.0	43.7231	16-17
AGTACTT	246985	0.0	43.498516	20-21
TATCAAC	315945	0.0	43.328533	10-11
GAGTACT	239475	0.0	43.20549	20-21
GCAGAGT	323705	0.0	43.116077	16-17
>>END_MODULE