FastQCFastQC Report
Thu 26 May 2016
SRR1660589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26312590
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12171064.625565176214124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660530.6310781264786173No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT336310.127813339545822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1499400.083.43628
AGTGGTA1529000.082.303445
GTGGTAT1524950.082.238546
GTATCAA1528750.081.689889
TGGTATC1531450.081.662457
AAGCAGT1615000.079.051071
GCAGTGG1632400.077.8414153
CAGTGGT1637050.077.2041244
AGCAGTG1715850.074.64772
CAACGCA1472650.044.79392212-13
ATCAACG1488300.044.21870810-11
ACGCAGA1511250.043.7850514-15
AGAGTAC1528350.042.83131418-19
TCAACGC1470550.042.4486812-13
AACGCAG1494100.041.66953314-15
AGTACTT1577150.041.2016420-21
GAGTACT1486400.040.76061220-21
CGCAGAG1527450.040.66129316-17
TATCAAC1545900.040.3659810-11
GCAGAGT1660450.039.56209616-17
Produced by FastQC (version 0.11.5)