##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26312590
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48863380609814	34.0	31.0	34.0	31.0	34.0
2	32.77422507628477	34.0	31.0	34.0	31.0	34.0
3	32.85755298129146	34.0	31.0	34.0	31.0	34.0
4	36.26870779349353	37.0	37.0	37.0	35.0	37.0
5	36.18525812168244	37.0	37.0	37.0	35.0	37.0
6	36.17722440094267	37.0	37.0	37.0	35.0	37.0
7	36.10072440607329	37.0	36.0	37.0	35.0	37.0
8	36.14068117201689	37.0	36.0	37.0	35.0	37.0
9	37.91247687893894	39.0	38.0	39.0	35.0	39.0
10-11	37.81995493412089	39.0	38.0	39.0	35.0	39.0
12-13	37.87651168129021	39.0	38.0	39.0	35.0	39.0
14-15	39.38295620081489	41.0	39.0	41.0	36.0	41.0
16-17	39.35588866394376	41.0	39.0	41.0	36.0	41.0
18-19	39.310845625611165	41.0	39.0	41.0	36.0	41.0
20-21	39.24683902268838	41.0	39.0	41.0	36.0	41.0
22-23	39.108139297575796	40.5	39.0	41.0	35.5	41.0
24-25	39.14042560994565	41.0	39.0	41.0	36.0	41.0
26-27	39.1274591174795	41.0	39.0	41.0	35.5	41.0
28-29	39.071319414014354	41.0	39.0	41.0	35.0	41.0
30-31	38.98416649596258	40.5	39.0	41.0	35.0	41.0
32-33	38.92672783256988	40.0	38.5	41.0	35.0	41.0
34-35	38.79524155546831	40.0	38.0	41.0	35.0	41.0
36-37	38.66720984137251	40.0	38.0	41.0	35.0	41.0
38-39	38.356434942360295	40.0	38.0	41.0	34.0	41.0
40-41	38.245547891712675	40.0	37.5	41.0	34.0	41.0
42-43	38.14486726696232	40.0	37.0	41.0	34.0	41.0
44-45	38.05598614579561	40.0	37.0	41.0	33.5	41.0
46-47	37.92958260665332	40.0	37.0	41.0	33.0	41.0
48-49	37.782199262786364	40.0	36.5	41.0	33.0	41.0
50-51	37.61207057154009	40.0	36.0	41.0	33.0	41.0
52-53	37.295785135556784	40.0	36.0	41.0	32.5	41.0
54-55	36.73053388130929	39.0	35.0	41.0	31.0	41.0
56-57	36.21315849180944	39.0	35.0	41.0	30.0	41.0
58-59	36.14552043337429	39.0	35.0	41.0	30.0	41.0
60-61	35.946943212355755	39.0	35.0	41.0	30.0	41.0
62-63	35.644064476359034	38.5	35.0	40.5	29.5	41.0
64-65	35.52415875822182	38.0	35.0	40.0	30.0	41.0
66-67	35.282803593260866	37.0	35.0	40.0	30.0	41.0
68-69	34.972440797352135	37.0	35.0	39.5	29.0	41.0
70-71	34.62618873702665	36.0	35.0	39.0	29.0	41.0
72-73	34.2708099810775	36.0	34.5	39.0	29.0	41.0
74-75	33.90554943470027	35.0	34.0	38.5	29.0	40.0
76-77	32.34757992276701	34.5	32.0	36.5	26.5	39.0
78-79	33.21875719950032	35.0	34.0	37.0	28.5	39.0
80-81	33.12245998968555	35.0	34.0	36.0	29.0	39.0
82-83	32.871060659554985	35.0	34.0	36.0	29.0	37.5
84-85	32.63133336551058	35.0	34.0	36.0	28.0	37.0
86-87	32.41514510734215	35.0	34.0	35.0	27.5	37.0
88-89	32.223575520311755	35.0	34.0	35.0	27.0	36.0
90-91	32.04225918087121	35.0	34.0	35.0	27.0	36.0
92-93	31.865615300508235	35.0	34.0	35.0	26.0	36.0
94-95	31.726208784464013	35.0	34.0	35.0	25.5	36.0
96-97	31.548197706877204	35.0	34.0	35.0	25.0	35.5
98-99	31.39581971976153	35.0	33.5	35.0	25.0	35.0
100	31.297990011625615	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5255294469789433
1101	2	0.3858233552892685
1101	3	0.3304272791629117
1101	4	0.21952108566042483
1101	5	0.24375709706276893
1101	6	0.21102363351249664
1101	7	0.23854933890125096
1101	8	0.2185780093236218
1101	9	0.3183127290570269
1101	10-11	0.296151351047655
1101	12-13	0.3047919219321429
1101	14-15	0.40293984694577745
1101	16-17	0.39904805727413617
1101	18-19	0.36306531463370817
1101	20-21	0.3334411027233273
1101	22-23	0.2086339964665811
1101	24-25	0.3388173043164002
1101	26-27	0.39742694837893566
1101	28-29	0.4309419045713554
1101	30-31	0.38413131175612136
1101	32-33	0.3760218475433703
1101	34-35	0.41283652319847874
1101	36-37	0.3856607971739834
1101	38-39	0.31593426559801685
1101	40-41	-0.011966039730772593
1101	42-43	0.07066715596022277
1101	44-45	0.26220262376513404
1101	46-47	0.28615691458752224
1101	48-49	0.33164924588004396
1101	50-51	0.3510786833318065
1101	52-53	0.2601368306283689
1101	54-55	0.15733126445531553
1101	56-57	0.1447109405220388
1101	58-59	0.12161640230567627
1101	60-61	0.1573170384613789
1101	62-63	0.11522478293428406
1101	64-65	0.16599526161544986
1101	66-67	0.13220297385526436
1101	68-69	0.1418422868823228
1101	70-71	0.12906149794058308
1101	72-73	0.07809403573616436
1101	74-75	0.04962425588205832
1101	76-77	-0.006163219667300268
1101	78-79	0.12495995205163979
1101	80-81	0.17055199531564114
1101	82-83	0.17302700614634858
1101	84-85	0.18133943481144144
1101	86-87	0.16138185188707155
1101	88-89	0.1460070892522083
1101	90-91	0.162714548218311
1101	92-93	0.09350097913731403
1101	94-95	0.09698231523277556
1101	96-97	0.0782792183723835
1101	98-99	0.1518022516470765
1101	100	0.14219848312248118
1102	1	0.4745857515377949
1102	2	0.3196920384803832
1102	3	0.24404016966917652
1102	4	0.17882329472652003
1102	5	0.2234519643655659
1102	6	0.21755925306272417
1102	7	0.21809276002966982
1102	8	0.2304192869424
1102	9	0.2565383883462715
1102	10-11	0.21700892430242646
1102	12-13	0.2809898599354952
1102	14-15	0.31757111541613625
1102	16-17	0.2948497994858599
1102	18-19	0.2739561172398979
1102	20-21	0.2562981769798256
1102	22-23	0.27010448332904957
1102	24-25	0.32929579156390787
1102	26-27	0.3267628591853722
1102	28-29	0.26931135348144153
1102	30-31	0.3103078323117501
1102	32-33	0.2790909195438189
1102	34-35	0.3799450234415005
1102	36-37	0.4054861261508762
1102	38-39	0.36416499193530427
1102	40-41	0.3808316025851113
1102	42-43	0.3619847196832424
1102	44-45	0.34221631722711976
1102	46-47	0.3231588621421011
1102	48-49	0.3133252711725305
1102	50-51	0.30460629246395854
1102	52-53	0.3575562403814345
1102	54-55	0.3627588184911801
1102	56-57	0.2302341890265751
1102	58-59	0.17865774880344532
1102	60-61	0.1634074010243154
1102	62-63	0.14040353596826094
1102	64-65	0.17695130078406862
1102	66-67	0.1303004807051238
1102	68-69	0.12083597201143448
1102	70-71	0.10733561517085377
1102	72-73	0.034862658069272356
1102	74-75	0.09026286279317475
1102	76-77	-0.1533984477309005
1102	78-79	0.13510782995399495
1102	80-81	0.1502262321826322
1102	82-83	-0.021358322737476954
1102	84-85	0.07864023659845998
1102	86-87	0.16179600827200602
1102	88-89	0.1896524815328462
1102	90-91	0.18861294367187753
1102	92-93	0.19438853227492459
1102	94-95	0.14582823315012305
1102	96-97	0.1966720802506856
1102	98-99	0.21492721476156973
1102	100	0.2129809567198251
1103	1	0.4293284358203664
1103	2	0.2920488397422787
1103	3	0.25039955661648605
1103	4	0.18632244389497288
1103	5	0.16596963599956638
1103	6	0.17009618543592353
1103	7	0.20582557722508454
1103	8	0.2144888882052669
1103	9	0.24879989316720952
1103	10-11	0.2208580955485857
1103	12-13	0.23409764211784534
1103	14-15	0.2793764748711496
1103	16-17	0.3087119867162187
1103	18-19	0.2976487429658121
1103	20-21	0.30320793668417423
1103	22-23	0.32043233818886563
1103	24-25	0.3121698360043368
1103	26-27	0.2784158240762409
1103	28-29	0.31708077268728374
1103	30-31	0.330934798577303
1103	32-33	0.3111234830298457
1103	34-35	0.36641350099311865
1103	36-37	0.32683418450336177
1103	38-39	0.29062922966817695
1103	40-41	0.255368057467372
1103	42-43	0.28213270845305516
1103	44-45	0.29590568932687944
1103	46-47	0.2907112161009806
1103	48-49	0.3362723027428203
1103	50-51	0.33226925073835645
1103	52-53	0.35005983977553967
1103	54-55	0.3003702224182021
1103	56-57	0.2740133159673519
1103	58-59	0.2781747781459032
1103	60-61	0.24507809031410943
1103	62-63	0.24841701099198588
1103	64-65	0.2286317765405741
1103	66-67	0.22314257976407248
1103	68-69	0.243960069149324
1103	70-71	0.20895379264219116
1103	72-73	0.21173721885301688
1103	74-75	0.25837116009166294
1103	76-77	0.2650926478272524
1103	78-79	0.2539800835988615
1103	80-81	0.2834582855988117
1103	82-83	0.3008275328167187
1103	84-85	0.27317200012483767
1103	86-87	0.27813110542877695
1103	88-89	0.2544345794887022
1103	90-91	0.2598115199965463
1103	92-93	0.25407750291745046
1103	94-95	0.25031174095233766
1103	96-97	0.26438651497620214
1103	98-99	0.3162374513963577
1103	100	0.33351003220773734
1104	1	0.3877202327968092
1104	2	0.2577924330101169
1104	3	0.19685412122906598
1104	4	0.17035813168619285
1104	5	0.15994136161981487
1104	6	0.17848038494509666
1104	7	0.16368263091813873
1104	8	0.1505044404035516
1104	9	0.24105480774407795
1104	10-11	0.1870359603634526
1104	12-13	0.1967895454687678
1104	14-15	0.23725382357680047
1104	16-17	0.26498290769038135
1104	18-19	0.2683004038995094
1104	20-21	0.2544797803214607
1104	22-23	0.26690602312718426
1104	24-25	0.27579337778582413
1104	26-27	0.2353720518963982
1104	28-29	0.22006566787332815
1104	30-31	0.3132924648260911
1104	32-33	0.2927635397859518
1104	34-35	0.3695376638735155
1104	36-37	0.2586078679734385
1104	38-39	0.3071589338082177
1104	40-41	0.30514597161809576
1104	42-43	0.32216227887250426
1104	44-45	0.22229331685540643
1104	46-47	0.2522771361595275
1104	48-49	0.3200057806108134
1104	50-51	0.3204716869027848
1104	52-53	0.2733202547081106
1104	54-55	0.29242032177435107
1104	56-57	0.17147758278802172
1104	58-59	0.145032462305565
1104	60-61	0.13543355711200888
1104	62-63	0.12813680590885212
1104	64-65	0.1078378011771477
1104	66-67	0.13019478719885313
1104	68-69	0.12830910130570317
1104	70-71	0.13593201199097393
1104	72-73	0.15317053112046608
1104	74-75	0.1788631957346709
1104	76-77	-0.10766180290477934
1104	78-79	0.19359156583155368
1104	80-81	0.21612787516451704
1104	82-83	0.22321900233119152
1104	84-85	0.20086625940585634
1104	86-87	0.09608533915217521
1104	88-89	0.15941231229361108
1104	90-91	0.19457089734039812
1104	92-93	0.20646875110403684
1104	94-95	0.23096333721617412
1104	96-97	0.27678780576681916
1104	98-99	0.2905736405747241
1104	100	0.2949980106760677
1105	1	0.37762573448190295
1105	2	0.26514896467941895
1105	3	0.20513273757433836
1105	4	0.152123881631546
1105	5	0.12866516413633633
1105	6	0.1186900414552241
1105	7	0.14608508487569338
1105	8	0.1317743117068204
1105	9	0.22312542305212446
1105	10-11	0.20613523971859848
1105	12-13	0.1961501230512468
1105	14-15	0.24857944254691233
1105	16-17	0.2629148511162569
1105	18-19	0.20274841992668513
1105	20-21	0.19717949768714504
1105	22-23	0.24338320057781004
1105	24-25	0.167605096324138
1105	26-27	0.19731465147106064
1105	28-29	0.17374496358840474
1105	30-31	0.20032506237772196
1105	32-33	0.20525696651426983
1105	34-35	0.15462565238612314
1105	36-37	0.13959282245831872
1105	38-39	0.13766599822908177
1105	40-41	0.1590677482879741
1105	42-43	0.25699076625918593
1105	44-45	0.2440369084045173
1105	46-47	0.2736502956873679
1105	48-49	0.2767842651473629
1105	50-51	0.19876590234387237
1105	52-53	0.21064520657117924
1105	54-55	0.18633087913446644
1105	56-57	0.17765681583207282
1105	58-59	0.11689160723656045
1105	60-61	0.059011214007909985
1105	62-63	0.070744597265616
1105	64-65	0.020264337571127555
1105	66-67	0.08807613395671154
1105	68-69	0.12604689710292405
1105	70-71	0.0984639043539346
1105	72-73	0.08858790493177082
1105	74-75	0.008677222482504021
1105	76-77	-0.07377405939194404
1105	78-79	0.13846389259329328
1105	80-81	0.09913783802016951
1105	82-83	0.13216890112348523
1105	84-85	0.11557660034736728
1105	86-87	0.09817946128131183
1105	88-89	0.12005626858005769
1105	90-91	0.128537048284457
1105	92-93	0.1293871001083211
1105	94-95	0.16425058779150703
1105	96-97	0.11173902695750826
1105	98-99	0.16405653090778927
1105	100	0.16490512344542196
1106	1	0.4025717958664785
1106	2	0.25087493482347867
1106	3	0.2170129739193456
1106	4	0.16086351343058425
1106	5	0.16856602223088402
1106	6	0.15786860651484602
1106	7	0.13922635712748388
1106	8	0.16727348286526933
1106	9	0.18359016205931056
1106	10-11	0.18096369388194233
1106	12-13	0.23231865924825712
1106	14-15	0.2581793740981979
1106	16-17	0.23228099896696364
1106	18-19	0.22176401749523222
1106	20-21	0.2631378357590961
1106	22-23	0.28962007873047924
1106	24-25	0.2525366706590404
1106	26-27	0.23734361217690747
1106	28-29	0.230058630273831
1106	30-31	0.3083847356603968
1106	32-33	0.2481604200739156
1106	34-35	0.21242706690612323
1106	36-37	0.25113718032248045
1106	38-39	0.18491644357310122
1106	40-41	0.2697199487568156
1106	42-43	0.2769183010723637
1106	44-45	0.21844840766170392
1106	46-47	0.24977287796694725
1106	48-49	0.29063625213942856
1106	50-51	0.283138405611588
1106	52-53	0.25570896302266277
1106	54-55	0.18961690725036107
1106	56-57	0.22729551292569994
1106	58-59	0.2509250943969761
1106	60-61	0.2800247938049196
1106	62-63	0.26195976631277773
1106	64-65	0.22950160677487474
1106	66-67	0.20333193796492566
1106	68-69	0.19273634980514487
1106	70-71	0.2314349380149352
1106	72-73	0.20718743471718426
1106	74-75	0.20552155496596924
1106	76-77	-0.5711750308389156
1106	78-79	0.18750086895977347
1106	80-81	0.18257969094753435
1106	82-83	0.208474035355799
1106	84-85	0.23942426714491205
1106	86-87	0.21956062278295718
1106	88-89	0.2242321685283386
1106	90-91	0.2673627550451627
1106	92-93	0.26198886054327275
1106	94-95	0.2404355940653815
1106	96-97	0.30972420908815934
1106	98-99	0.3522369837037225
1106	100	0.3919641463944501
1107	1	0.49238166935524674
1107	2	0.36484697567266267
1107	3	0.29485341016128075
1107	4	0.19098578715345127
1107	5	0.2132744541155418
1107	6	0.21090477220383264
1107	7	0.16824749721131127
1107	8	0.22424043000020788
1107	9	0.2534560559850618
1107	10-11	0.27546882299969155
1107	12-13	0.3064807430260643
1107	14-15	0.31997102162568325
1107	16-17	0.3388335786460672
1107	18-19	0.3213208928609532
1107	20-21	0.3366351702233459
1107	22-23	0.39283138547983043
1107	24-25	0.402651439963563
1107	26-27	0.42563801964950443
1107	28-29	0.4046679424616997
1107	30-31	0.4335185156758996
1107	32-33	0.4228129223797765
1107	34-35	0.4255989260458648
1107	36-37	0.3818199260526569
1107	38-39	0.432056151266643
1107	40-41	0.4852965182966571
1107	42-43	0.3916115090575545
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2314	100	-0.3158533075333061
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2316	3	-1.2126855260697376
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2316	64-65	-0.5838905320886596
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2316	80-81	-0.5337849429301613
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2316	84-85	-0.5116056095306192
2316	86-87	-0.5037516915329228
2316	88-89	-0.5387528157137567
2316	90-91	-0.5691081844547021
2316	92-93	-0.5594824482232887
2316	94-95	-0.5867729072954759
2316	96-97	-0.5552460361793408
2316	98-99	-0.5304736558334149
2316	100	-0.5667282020603146
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1160.0
3	339.0
4	478.0
5	905.0
6	1598.0
7	2642.0
8	5007.0
9	8052.0
10	11688.0
11	16090.0
12	20280.0
13	25946.0
14	28419.0
15	29062.0
16	36606.0
17	50865.0
18	74325.0
19	123791.0
20	272651.0
21	347495.0
22	131388.0
23	95835.0
24	107225.0
25	124017.0
26	145848.0
27	179276.0
28	226484.0
29	283195.0
30	349467.0
31	434635.0
32	544823.0
33	706249.0
34	973068.0
35	1607323.0
36	2859580.0
37	5949882.0
38	8036182.0
39	2494515.0
40	6199.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.893584424971786	36.48150043997287	22.083083650424136	26.541831484631214
2	21.549661109291332	32.32696794052315	29.569467673130884	16.553903277054644
3	26.057883317453733	27.61866467725146	24.968123624470262	21.355328380824538
4	20.60181456861525	27.265248308889394	25.509442438011614	26.623494684483738
5	22.08290403947312	32.36219619581349	25.15467310515612	20.40022665955727
6	26.402383801822626	26.43717703198355	25.67404425029995	21.486394915893875
7	21.7283285301827	26.658496940057972	30.772329899869227	20.8408446298901
8	24.969259962626257	25.986233206233212	27.54683214385205	21.49767468728848
9	25.179315301154315	25.44402128410772	28.267589773564673	21.109073641173293
10-11	20.676294123839575	29.08478032759223	29.143896514938287	21.09502903362991
12-13	20.86080275639912	26.74446339185918	28.425882818833117	23.96885103290858
14-15	21.057185932665693	31.82611821945312	25.507561209291822	21.60913463858936
16-17	24.278351161934268	26.378085547640882	25.252713624922517	24.090849665502333
18-19	21.641786308379373	28.99755022215601	25.142156283360933	24.218507186103686
20-21	24.25631418267833	28.97664578059401	25.331624518909006	21.435415517818658
22-23	23.898861723608356	26.471996865378895	28.2368858405805	21.392255570432255
24-25	21.34635078243345	28.666541293257623	25.75056243200494	24.23654549230398
26-27	21.32606032880164	26.171767138733642	31.085403662985062	21.416768869479665
28-29	21.42995158980929	26.252448666046668	30.866206436412142	21.451393307731898
30-31	21.534239123151814	26.182965437597723	30.694018466494587	21.58877697275588
32-33	21.548537445016322	26.271691316888397	30.60399335340876	21.575777884686524
34-35	21.681171929183094	26.30307244541469	30.468677279687384	21.547078345714837
36-37	21.602278093927403	26.276476294643498	30.480385129157224	21.640860482271872
38-39	21.610375489452007	26.29235282425637	30.59853096939526	21.49874071689636
40-41	21.571743792610306	26.34242391189921	30.61646344962621	21.46936884586428
42-43	21.5019768103406	26.292938095413643	30.607275832595725	21.59780926165003
44-45	21.512692213119273	26.312774607136735	30.692200957792448	21.482332221951545
46-47	21.492570666741663	26.417838380790336	30.598038809558464	21.491552142909534
48-49	21.56625402516438	26.359721714966106	30.465761827322968	21.60826243254655
50-51	21.580585567593307	26.407845065803098	30.47825964680786	21.53330971979573
52-53	21.6485656157687	26.553230783462826	30.205865133100403	21.592338467668068
54-55	21.84907998860475	26.69168142316928	29.63279589231127	21.826442695914704
56-57	22.109136319812542	27.133980470451636	28.859018080871824	21.897865128863998
58-59	22.193784799925595	27.52333468165994	28.387824587956178	21.895055930458287
60-61	22.146564088991912	27.64624999809872	28.23309232751238	21.97409358539698
62-63	22.052805444773202	27.766432514861783	28.251499769959267	21.929262270405747
64-65	22.029269773952652	27.88040649053926	28.179542284363425	21.910781451144658
66-67	21.957607581042407	27.68656644946513	28.369464083760388	21.98636188573208
68-69	21.871672457937436	27.591464770286773	28.678731360158764	21.858131411617023
70-71	21.89169519230148	27.52156667207599	28.800215030143367	21.786523105479162
72-73	21.84668457813027	27.427439009524402	28.884917234334168	21.840959178011165
74-75	21.8482918874435	27.512582289057846	28.885436970246285	21.753688853252367
76-77	21.879528013015822	27.651130124400524	28.696200184018373	21.77314167856528
78-79	21.92764191726166	27.742544554204084	28.460265796670196	21.869547731864056
80-81	21.9549570553171	27.917551347907455	28.317890337214003	21.809601259561443
82-83	22.004002370169626	28.07376192950209	28.0571186546683	21.865117045659982
84-85	21.96292425095836	28.140954580995363	27.921749360103238	21.974371807943037
86-87	21.956064560371523	28.210617928666693	27.900441772148454	21.93287573881333
88-89	22.010247271378226	28.315568927080157	27.76972859082108	21.904455210720535
90-91	22.03707058473984	28.287226222495367	27.65602791917976	22.019675273585037
92-93	22.06168624359264	28.35893287637455	27.627348326584052	21.952032553448763
94-95	22.144513329930653	28.434990626160328	27.49605797072808	21.924438073180937
96-97	22.145640166931496	28.40190570369546	27.394101074808674	22.058353054564375
98-99	22.111930217737598	28.4353434717818	27.458540624903975	21.994185685576625
100	22.188490555747794	28.479507794090008	27.258079932534134	22.07392171762806
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129930.0
1	77470.5
2	20373.5
3	14727.0
4	13289.0
5	12452.5
6	11977.0
7	11451.0
8	10851.5
9	10547.0
10	10727.5
11	17514.5
12	178316.0
13	194740.0
14	53883.5
15	53262.5
16	58679.5
17	64686.5
18	70608.5
19	77372.5
20	86104.5
21	98459.0
22	116212.0
23	136824.0
24	158175.0
25	182714.0
26	211104.0
27	244792.0
28	286187.0
29	326425.0
30	359193.0
31	392106.0
32	427924.0
33	460906.5
34	493306.0
35	530622.5
36	573523.5
37	611157.5
38	650283.5
39	690302.0
40	712522.5
41	743026.5
42	784739.0
43	806197.0
44	824035.0
45	854486.0
46	885298.5
47	905026.5
48	912570.5
49	914359.5
50	913996.5
51	914032.5
52	910434.0
53	889966.5
54	853113.5
55	813713.5
56	767838.5
57	704589.0
58	629719.5
59	548982.0
60	454619.0
61	364645.5
62	289568.0
63	217496.5
64	157736.0
65	115058.0
66	82416.5
67	59426.0
68	44258.0
69	34337.0
70	28115.5
71	23721.0
72	20667.0
73	17024.0
74	13234.5
75	11171.5
76	8988.5
77	6214.5
78	4050.0
79	2538.0
80	1584.0
81	1114.5
82	722.0
83	390.0
84	239.5
85	158.5
86	89.5
87	47.5
88	25.5
89	21.0
90	18.5
91	13.0
92	6.5
93	2.5
94	3.0
95	3.0
96	1.5
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028727692712880032
2	0.011127752912199064
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007046056659568671
26-27	0.028349546737892396
28-29	0.03316853263019718
30-31	0.03010916067175447
32-33	0.03718372079677447
34-35	0.02974431631397745
36-37	2.5273072700178885E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.005535372990648203
54-55	0.03287209658950335
56-57	0.045730959970113165
58-59	0.050637356489802034
60-61	0.05499268601076519
62-63	0.03663455402907886
64-65	0.0011116351526018532
66-67	3.040369648141821E-5
68-69	0.0
70-71	0.0
72-73	7.030854811327961E-5
74-75	3.420415854159549E-5
76-77	0.0
78-79	5.548674607858823E-4
80-81	0.016666926364907447
82-83	0.04056993249239242
84-85	0.05473615482170322
86-87	0.05866583259192653
88-89	0.057949445493583114
90-91	0.036199401123188556
92-93	0.002515905883837357
94-95	0.0
96-97	0.0
98-99	4.3895336795047544E-4
100	9.50115515044319E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.631259E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.98209439891504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17518642185682	30.162764872457103
2	14.593295187981559	12.545007827221118
3	5.54057331339504	7.1443633554136525
4	2.780699487096121	4.7808115139752045
5	1.629698674186797	3.5023931127841794
6	1.116654872102367	2.8797699074227396
7	0.7343365963815611	2.2094327434375156
8	0.550293870410619	1.8922226468106835
9	0.4232862203820281	1.637435545399826
>10	2.212087260054563	17.00701282665305
>50	0.1697731409680748	5.084801290951493
>100	0.07291908868488808	5.080266536836172
>500	8.060104491216126E-4	0.22074250665868378
>1k	3.101915535117173E-4	0.29840406155361104
>5k	5.310966465759817E-5	0.16424110978595932
>10k+	2.6554832327864505E-5	5.390330142638988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1217106	4.625565176214124	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	166053	0.6310781264786173	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33631	0.127813339545822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8242217888850927E-4	1.140138618053183E-5	0.0	0.0	0.0
2	2.2802772361063658E-4	2.6603234421240936E-5	0.0	0.0	0.0
3	3.0783742687435936E-4	3.420415854159549E-5	0.0	0.0	3.8004620601772763E-6
4	3.7244528189737307E-4	3.420415854159549E-5	0.0	3.8004620601772763E-6	3.8004620601772763E-6
5	4.4845452310091863E-4	4.940600678230459E-5	0.0	3.8004620601772763E-6	3.8004620601772763E-6
6	5.130623781239323E-4	6.840831708319098E-5	0.0	3.8004620601772763E-6	7.6009241203545525E-6
7	5.928720813876551E-4	7.220877914336826E-5	0.0	3.8004620601772763E-6	7.6009241203545525E-6
8	7.14486867313328E-4	7.98097032637228E-5	0.0	7.6009241203545525E-6	7.6009241203545525E-6
9	8.361016532390008E-4	9.501155150443191E-5	0.0	7.6009241203545525E-6	7.6009241203545525E-6
10-11	0.0010983335353912328	1.045127066548751E-4	0.0	9.50115515044319E-6	9.50115515044319E-6
12-13	0.0014308739656567447	1.1401386180531829E-4	0.0	1.140138618053183E-5	1.7102079270797744E-5
14-15	0.0017710153200426108	1.3111594107611602E-4	0.0	1.140138618053183E-5	2.0902541330975018E-5
16-17	0.002171964067391313	1.501182513770024E-4	0.0	1.140138618053183E-5	3.040369648141821E-5
18-19	0.0026926273696356003	1.7102079270797744E-4	0.0	1.140138618053183E-5	3.610438957168412E-5
20-21	0.0031657848961276712	1.9192333403895246E-4	0.0	1.140138618053183E-5	3.800462060177276E-5
22-23	0.0036180398812887668	1.9762402712921836E-4	0.0	1.140138618053183E-5	4.180508266195004E-5
24-25	0.003999986318336583	2.0332472021948426E-4	0.0	1.140138618053183E-5	4.560554472212732E-5
26-27	0.004391433910534843	2.2992795464072524E-4	0.0	1.140138618053183E-5	4.940600678230459E-5
28-29	0.004725874571830443	2.489302649416116E-4	0.0	1.140138618053183E-5	5.320646884248187E-5
30-31	0.004984305991922498	2.831344234832071E-4	0.0	1.5201848240709105E-5	5.7006930902659146E-5
32-33	0.005242737412014553	3.154383509947139E-4	0.0	1.5201848240709105E-5	5.7006930902659146E-5
34-35	0.0054935679079862534	3.382411233557776E-4	0.0	1.5201848240709105E-5	6.270762399292506E-5
36-37	0.005786203486619903	3.5534320262657534E-4	0.0	1.900231030088638E-5	7.030854811327961E-5
38-39	0.006057936523922579	3.6104389571684127E-4	0.0	1.900231030088638E-5	7.600924120354552E-5
40-41	0.006394277416248267	3.78145974987639E-4	0.0	1.900231030088638E-5	7.98097032637228E-5
42-43	0.0067401194637244	3.971482852885254E-4	0.0	1.900231030088638E-5	8.9310858414166E-5
44-45	0.007190474217855407	4.180508266195004E-4	0.0	1.900231030088638E-5	1.0261247562478646E-4
46-47	0.007703536595979339	4.3135244383012085E-4	0.0	1.900231030088638E-5	1.1021339974514101E-4
48-49	0.008473130163165237	4.389533679504754E-4	0.0	1.900231030088638E-5	1.1401386180531829E-4
50-51	0.009465050760871507	4.5985590928145043E-4	0.0	1.900231030088638E-5	1.1781432386549557E-4
52-53	0.010740105782060983	4.7125729546198224E-4	0.0	1.900231030088638E-5	1.3301617210620468E-4
54-55	0.01230589615085402	4.8645914370269136E-4	0.0	2.47030033911523E-5	1.3681663416638195E-4
56-57	0.014278335960086028	4.978605298832232E-4	0.0	2.6603234421240936E-5	1.3871686519647058E-4
58-59	0.016780940226712764	5.3016445739473E-4	0.0	2.6603234421240936E-5	1.4251732725664788E-4
60-61	0.019874516343697066	5.510669987257051E-4	0.0	2.6603234421240936E-5	1.4631778931682515E-4
62-63	0.024039822761651362	5.757700021168573E-4	3.8004620601772763E-6	2.8503465451329573E-5	1.501182513770024E-4
64-65	0.029478283969765047	6.137746227186301E-4	3.8004620601772763E-6	3.040369648141821E-5	1.5581894446726834E-4
66-67	0.03636662145383636	6.213755468389846E-4	3.8004620601772763E-6	3.040369648141821E-5	1.6532009961771154E-4
68-69	0.04586777660427954	6.25176008899162E-4	3.8004620601772763E-6	3.040369648141821E-5	1.7102079270797744E-4
70-71	0.05830858915826986	6.346771640496052E-4	3.8004620601772763E-6	3.040369648141821E-5	1.7292102373806607E-4
72-73	0.07418311918363035	6.650808605310234E-4	3.8004620601772763E-6	3.040369648141821E-5	1.748212547681547E-4
74-75	0.09454409467103012	6.992850190726189E-4	3.8004620601772763E-6	3.040369648141821E-5	1.8242217888850927E-4
76-77	0.12014020664632406	7.296887155540371E-4	3.8004620601772763E-6	3.420415854159549E-5	1.8242217888850927E-4
78-79	0.1523776260717778	7.410901017345689E-4	3.8004620601772763E-6	3.420415854159549E-5	1.881228719787752E-4
80-81	0.19314708282232956	7.619926430655439E-4	3.8004620601772763E-6	3.420415854159549E-5	1.9192333403895246E-4
82-83	0.2441986896766909	7.847954154266076E-4	3.8004620601772763E-6	3.420415854159549E-5	1.9762402712921836E-4
84-85	0.3094469225568445	7.961968016071394E-4	3.8004620601772763E-6	3.420415854159549E-5	2.0522495124957293E-4
86-87	0.3910466434509108	8.208998049982917E-4	3.8004620601772763E-6	3.420415854159549E-5	2.0712518227966156E-4
88	0.46370197688634984	8.285007291186462E-4	3.8004620601772763E-6	3.420415854159549E-5	2.090254133097502E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	149940	0.0	83.4362	8
AGTGGTA	152900	0.0	82.30344	5
GTGGTAT	152495	0.0	82.23854	6
GTATCAA	152875	0.0	81.68988	9
TGGTATC	153145	0.0	81.66245	7
AAGCAGT	161500	0.0	79.05107	1
GCAGTGG	163240	0.0	77.841415	3
CAGTGGT	163705	0.0	77.204124	4
AGCAGTG	171585	0.0	74.6477	2
CAACGCA	147265	0.0	44.793922	12-13
ATCAACG	148830	0.0	44.218708	10-11
ACGCAGA	151125	0.0	43.78505	14-15
AGAGTAC	152835	0.0	42.831314	18-19
TCAACGC	147055	0.0	42.44868	12-13
AACGCAG	149410	0.0	41.669533	14-15
AGTACTT	157715	0.0	41.20164	20-21
GAGTACT	148640	0.0	40.760612	20-21
CGCAGAG	152745	0.0	40.661293	16-17
TATCAAC	154590	0.0	40.36598	10-11
GCAGAGT	166045	0.0	39.562096	16-17
>>END_MODULE