FastQCFastQC Report
Thu 26 May 2016
SRR1660588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29584962
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15150625.12105440595124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2095660.7083531153428556No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT531510.17965546144693376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2513950.083.475471
GTGGTAT2506450.082.810426
AGTGGTA2517700.082.495925
TGGTATC2512000.082.2402047
GGTATCA2523350.081.9960258
GCAGTGG2575000.081.0718463
GTATCAA2552700.080.9914559
CAGTGGT2603700.079.778714
AGCAGTG2631150.079.5946962
CAACGCA2532500.042.5575312-13
ATCAACG2556050.042.25881210-11
ACGCAGA2571100.042.09314-15
AGAGTAC2539100.041.48303218-19
TCAACGC2531600.040.74168812-13
AGTACTT2159900.040.5000120-21
AACGCAG2556850.040.41271614-15
TATCAAC2581950.039.97610-11
GAGTACT2070700.039.93894220-21
CGCAGAG2588650.039.87342516-17
GCAGAGT2711050.039.67474416-17
Produced by FastQC (version 0.11.5)