FastQCFastQC Report
Thu 26 May 2016
SRR1660588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29584962
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10615183.588032325341503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2259950.763884706020579No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT523910.17708658878791192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1481150.077.2714161
GTGGTAT1455150.076.278356
AGTGGTA1464450.076.03785
TGGTATC1477150.075.46047
GGTATCA1485550.075.055858
GCAGTGG1510850.074.0291443
GTATCAA1543450.073.1992349
CAGTGGT1550500.072.066344
AGCAGTG1587100.071.244922
CAACGCA1532550.039.89990612-13
ATCAACG1549650.039.3777510-11
ACGCAGA1562000.038.95961814-15
AGAGTAC1579950.038.1669218-19
AGTACTT1586750.037.80903620-21
GAGTACT1477350.037.0075220-21
TCAACGC1531700.036.93112-13
AACGCAG1540850.036.4021714-15
TATCAAC1579300.035.96964310-11
CGCAGAG1573650.035.4553116-17
GCAGAGT1709100.035.28886416-17
Produced by FastQC (version 0.11.5)