##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29584962
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.527551125467056	34.0	31.0	34.0	31.0	34.0
2	32.80903565128798	34.0	31.0	34.0	31.0	34.0
3	32.88957011335692	34.0	31.0	34.0	31.0	34.0
4	36.30358223884148	37.0	37.0	37.0	35.0	37.0
5	36.22426177190966	37.0	37.0	37.0	35.0	37.0
6	36.20922869530811	37.0	37.0	37.0	35.0	37.0
7	36.15741169449533	37.0	36.0	37.0	35.0	37.0
8	36.17629841809498	37.0	37.0	37.0	35.0	37.0
9	37.95245537242874	39.0	38.0	39.0	35.0	39.0
10-11	37.89133457396362	39.0	38.0	39.0	35.0	39.0
12-13	37.92000716445064	39.0	38.0	39.0	35.0	39.0
14-15	39.434036927274065	41.0	39.0	41.0	36.0	41.0
16-17	39.40494170653321	41.0	39.0	41.0	36.0	41.0
18-19	39.3610157755146	41.0	39.0	41.0	36.0	41.0
20-21	39.30255969231936	41.0	39.0	41.0	36.0	41.0
22-23	39.18926331559932	41.0	39.0	41.0	35.5	41.0
24-25	39.20975350585206	41.0	39.0	41.0	36.0	41.0
26-27	39.18048365247182	41.0	39.0	41.0	36.0	41.0
28-29	39.111175434330455	41.0	39.0	41.0	35.0	41.0
30-31	39.014246917065506	41.0	39.0	41.0	35.0	41.0
32-33	38.94380978079336	40.0	39.0	41.0	35.0	41.0
34-35	38.799979513240544	40.0	38.0	41.0	35.0	41.0
36-37	38.67197174361759	40.0	38.0	41.0	35.0	41.0
38-39	38.39545876381386	40.0	38.0	41.0	34.0	41.0
40-41	38.276550667869714	40.0	37.5	41.0	34.0	41.0
42-43	38.1729787586004	40.0	37.0	41.0	34.0	41.0
44-45	38.07036282487029	40.0	37.0	41.0	33.5	41.0
46-47	37.93400084475349	40.0	37.0	41.0	33.0	41.0
48-49	37.78218221135454	40.0	36.0	41.0	33.0	41.0
50-51	37.6181591512607	40.0	36.0	41.0	33.0	41.0
52-53	37.34420887544152	40.0	36.0	41.0	33.0	41.0
54-55	36.883888240248545	39.5	35.0	41.0	31.5	41.0
56-57	36.463787176742024	39.0	35.0	41.0	31.0	41.0
58-59	36.432940779170174	39.0	35.0	41.0	31.0	41.0
60-61	36.26049010980646	39.0	35.0	41.0	31.0	41.0
62-63	35.983033187603894	38.5	35.0	41.0	30.5	41.0
64-65	35.88975983812317	38.0	35.0	40.5	31.0	41.0
66-67	35.672341711981915	37.5	35.0	40.0	31.0	41.0
68-69	35.382473011119636	37.0	35.0	40.0	30.5	41.0
70-71	35.05538176794008	36.5	35.0	39.0	30.0	41.0
72-73	34.71332750064036	36.0	35.0	39.0	30.0	41.0
74-75	34.357010632631535	35.5	34.5	39.0	29.5	40.5
76-77	32.767369990199754	34.5	32.0	36.5	27.0	39.0
78-79	33.656621123934514	35.0	34.0	37.0	29.0	39.0
80-81	33.555178201682324	35.0	34.0	37.0	29.0	39.0
82-83	33.29172579298902	35.0	34.0	36.0	29.0	38.5
84-85	33.03756501360387	35.0	34.0	36.0	29.0	37.0
86-87	32.80788701029935	35.0	34.0	35.5	29.0	37.0
88-89	32.602354094624154	35.0	34.0	35.0	29.0	36.5
90-91	32.40704334857689	35.0	34.0	35.0	29.0	36.0
92-93	32.219408833447204	35.0	34.0	35.0	27.5	36.0
94-95	32.073048969946285	35.0	34.0	35.0	27.0	36.0
96-97	31.89128017132488	35.0	34.0	35.0	27.0	36.0
98-99	31.73588445035015	35.0	34.0	35.0	26.5	35.0
100	31.637739267672544	35.0	34.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5039669144800669
1101	2	0.37858735694393175
1101	3	0.3200214839114466
1101	4	0.20504903655039897
1101	5	0.21600221393130425
1101	6	0.19310582149952182
1101	7	0.23650627507737454
1101	8	0.2048922747369204
1101	9	0.30808060644637436
1101	10-11	0.2801409555242813
1101	12-13	0.306388257110747
1101	14-15	0.4150601554102451
1101	16-17	0.3987722245532268
1101	18-19	0.3411769649929397
1101	20-21	0.32261374678658683
1101	22-23	0.19496307122085454
1101	24-25	0.3007775335689118
1101	26-27	0.35261570658500574
1101	28-29	0.37538436654129725
1101	30-31	0.34967406837936466
1101	32-33	0.32842356585529586
1101	34-35	0.3787246702583076
1101	36-37	0.3422329020076873
1101	38-39	0.2978193152599431
1101	40-41	-0.03948598716844742
1101	42-43	0.05445319639191126
1101	44-45	0.2513975327293849
1101	46-47	0.26945405680037027
1101	48-49	0.26115921411916077
1101	50-51	0.31036135849740987
1101	52-53	0.2632378124875103
1101	54-55	0.1611834761637283
1101	56-57	0.17255650894077945
1101	58-59	0.15407153871370838
1101	60-61	0.18133647273745623
1101	62-63	0.12327788328419587
1101	64-65	0.18096802346615704
1101	66-67	0.1765379331292749
1101	68-69	0.1956013951329325
1101	70-71	0.2302608271346216
1101	72-73	0.14972244023677916
1101	74-75	0.07356605811781947
1101	76-77	0.020429603033434773
1101	78-79	0.16166188501069456
1101	80-81	0.20112969170827455
1101	82-83	0.20413056917556816
1101	84-85	0.22508015012835614
1101	86-87	0.1980767520127813
1101	88-89	0.16440729895283823
1101	90-91	0.1660243137277888
1101	92-93	0.08268101466564559
1101	94-95	0.1078052859265739
1101	96-97	0.10103865144826685
1101	98-99	0.17927662148156642
1101	100	0.1393198537689564
1102	1	0.4577104695948364
1102	2	0.3071159627374058
1102	3	0.24863540403362805
1102	4	0.17873026454200414
1102	5	0.2005047173134784
1102	6	0.20611487244952542
1102	7	0.2112588090847538
1102	8	0.23597462034518912
1102	9	0.27833524435705215
1102	10-11	0.2295662474053799
1102	12-13	0.2822968555649723
1102	14-15	0.33470961622301587
1102	16-17	0.2908318849768463
1102	18-19	0.2763684476711106
1102	20-21	0.2574711453816576
1102	22-23	0.2987457663527593
1102	24-25	0.3375034609858787
1102	26-27	0.33666473258163165
1102	28-29	0.2648764278578284
1102	30-31	0.31880462339811544
1102	32-33	0.3039018973198253
1102	34-35	0.3814831997900683
1102	36-37	0.3926306877283565
1102	38-39	0.37327355713459553
1102	40-41	0.3725497104901123
1102	42-43	0.3507247221340535
1102	44-45	0.3390370906615985
1102	46-47	0.3005660757637969
1102	48-49	0.2908187304743066
1102	50-51	0.26595365025200124
1102	52-53	0.31305566116330397
1102	54-55	0.29388270338276357
1102	56-57	0.21980009590203053
1102	58-59	0.15503927855239397
1102	60-61	0.14130801466802723
1102	62-63	0.1068427662368876
1102	64-65	0.21593495422961695
1102	66-67	0.2065031775932411
1102	68-69	0.20951550753645876
1102	70-71	0.17710531715445654
1102	72-73	0.14889119367559545
1102	74-75	0.19479769917007417
1102	76-77	-0.10751353924667484
1102	78-79	0.24646187556057697
1102	80-81	0.2241097833903538
1102	82-83	0.05343582398172941
1102	84-85	0.1357817105528838
1102	86-87	0.1784198504842962
1102	88-89	0.22593039524568326
1102	90-91	0.22327136828788952
1102	92-93	0.1982420894954373
1102	94-95	0.1806884931132302
1102	96-97	0.2621581077282009
1102	98-99	0.27749296598121376
1102	100	0.2623419920928427
1103	1	0.4397456714449177
1103	2	0.31983430740618957
1103	3	0.2712538998224403
1103	4	0.21536739047648723
1103	5	0.18090136373361787
1103	6	0.19386246203367108
1103	7	0.21929473619950102
1103	8	0.23103136481221043
1103	9	0.2557452652165111
1103	10-11	0.24980259943342986
1103	12-13	0.2540269626905598
1103	14-15	0.28645539048154234
1103	16-17	0.3081524605359718
1103	18-19	0.3050737254814777
1103	20-21	0.3360386296380824
1103	22-23	0.3340180949868099
1103	24-25	0.3519098438637869
1103	26-27	0.2983416773318197
1103	28-29	0.3016258714433562
1103	30-31	0.3521777555380652
1103	32-33	0.3166928421397799
1103	34-35	0.38530011547199194
1103	36-37	0.3507660947090159
1103	38-39	0.29465693099447776
1103	40-41	0.2688096694922848
1103	42-43	0.2996814419968885
1103	44-45	0.2794276765952546
1103	46-47	0.279644644503648
1103	48-49	0.3188001431925329
1103	50-51	0.30804323852516546
1103	52-53	0.2705840709164917
1103	54-55	0.21870776016123727
1103	56-57	0.17789929808844818
1103	58-59	0.17541625949552042
1103	60-61	0.2071871488234791
1103	62-63	0.19403413762253052
1103	64-65	0.19139360888182466
1103	66-67	0.1684042050136938
1103	68-69	0.18234413024261187
1103	70-71	0.14012468079287288
1103	72-73	0.1761683840805759
1103	74-75	0.17907172257979198
1103	76-77	0.20289162545286388
1103	78-79	0.2110320807388817
1103	80-81	0.22940782430974593
1103	82-83	0.22551464897758677
1103	84-85	0.23149053980392154
1103	86-87	0.23657976636805955
1103	88-89	0.22578019161399254
1103	90-91	0.224634844353659
1103	92-93	0.20852051391242554
1103	94-95	0.2128480860313431
1103	96-97	0.2636382780348434
1103	98-99	0.27499478859691706
1103	100	0.2700597642054312
1104	1	0.39126961201537114
1104	2	0.2641941327929942
1104	3	0.18017307729338938
1104	4	0.1586277672249281
1104	5	0.15130447808120095
1104	6	0.1581725039288031
1104	7	0.15527670483032097
1104	8	0.13851061039252244
1104	9	0.19155249821836406
1104	10-11	0.1699818514111726
1104	12-13	0.16614990814057506
1104	14-15	0.22677025921873195
1104	16-17	0.25164156532942883
1104	18-19	0.28331689300907215
1104	20-21	0.23834438264630364
1104	22-23	0.2782333832901642
1104	24-25	0.2746452096737144
1104	26-27	0.2555575383995503
1104	28-29	0.231632936893007
1104	30-31	0.2998931671974745
1104	32-33	0.2819710321416622
1104	34-35	0.35826982652868367
1104	36-37	0.2310614310250827
1104	38-39	0.2969861664249578
1104	40-41	0.293658361829074
1104	42-43	0.2941135797186405
1104	44-45	0.19033130874102966
1104	46-47	0.25997139833248184
1104	48-49	0.2701298826166081
1104	50-51	0.2305253561092826
1104	52-53	0.23438059603144268
1104	54-55	0.2370989869009037
1104	56-57	0.1161842429823281
1104	58-59	0.1204916379730463
1104	60-61	0.16558620149920955
1104	62-63	0.09577875794234814
1104	64-65	0.09130351918107493
1104	66-67	0.1475659893731489
1104	68-69	0.14212199341963583
1104	70-71	0.14203956661383899
1104	72-73	0.1487844853191973
1104	74-75	0.176484925995247
1104	76-77	-0.13517467014128215
1104	78-79	0.1543623697122385
1104	80-81	0.22285753384680618
1104	82-83	0.21098740691588347
1104	84-85	0.19171779119243837
1104	86-87	0.06564207881503137
1104	88-89	0.15260380833510112
1104	90-91	0.1856726995747664
1104	92-93	0.20462682696742718
1104	94-95	0.23696468512044078
1104	96-97	0.31842761739686765
1104	98-99	0.34588863423836
1104	100	0.3536577413915154
1105	1	0.37771342097858707
1105	2	0.2594851646317764
1105	3	0.18021177499640828
1105	4	0.13553375299004955
1105	5	0.12218906299717958
1105	6	0.11428265067143428
1105	7	0.1268297937856886
1105	8	0.12543801482657813
1105	9	0.16939604759808446
1105	10-11	0.16216919702862498
1105	12-13	0.19879495699658634
1105	14-15	0.22313399364490039
1105	16-17	0.24419963319581228
1105	18-19	0.22373454472841559
1105	20-21	0.20495839320219034
1105	22-23	0.2457579202940181
1105	24-25	0.16357143032420396
1105	26-27	0.2116339360257271
1105	28-29	0.19576408834109316
1105	30-31	0.2364086269953063
1105	32-33	0.21738804501895714
1105	34-35	0.16695600497554608
1105	36-37	0.13175106525309133
1105	38-39	0.11569552433631713
1105	40-41	0.17034253036278812
1105	42-43	0.24531983444448002
1105	44-45	0.24252587175868712
1105	46-47	0.24098151545420876
1105	48-49	0.26999816445297853
1105	50-51	0.22242018049755785
1105	52-53	0.24211579980254072
1105	54-55	0.2349622354938532
1105	56-57	0.22821493158831885
1105	58-59	0.1885721376687215
1105	60-61	0.15032376040979045
1105	62-63	0.18941829321659753
1105	64-65	0.1531981386674417
1105	66-67	0.21331414684791383
1105	68-69	0.20612884669528597
1105	70-71	0.18327324982274718
1105	72-73	0.21898421705175508
1105	74-75	0.159182957273039
1105	76-77	0.01116847266990817
1105	78-79	0.23290380781141806
1105	80-81	0.23946060667191915
1105	82-83	0.23616525689605083
1105	84-85	0.22355086294631832
1105	86-87	0.22139758348689753
1105	88-89	0.2297814464035568
1105	90-91	0.2348172392315533
1105	92-93	0.24851225480568928
1105	94-95	0.2785044367074221
1105	96-97	0.2517052418462775
1105	98-99	0.25069283621166605
1105	100	0.2770131377991696
1106	1	0.3921274294094488
1106	2	0.24525645198046675
1106	3	0.18223440843544125
1106	4	0.1408904589246589
1106	5	0.15737661220714472
1106	6	0.12972089084060912
1106	7	0.10787567140206988
1106	8	0.13153620029588353
1106	9	0.19779499651278343
1106	10-11	0.15453330980981406
1106	12-13	0.2086694981439976
1106	14-15	0.2249624483099879
1106	16-17	0.2151743777565187
1106	18-19	0.16514522529505626
1106	20-21	0.23458956449786683
1106	22-23	0.2919489660849237
1106	24-25	0.22388674058655766
1106	26-27	0.20858257646491296
1106	28-29	0.2163244752568403
1106	30-31	0.32038788597294143
1106	32-33	0.2630797544186336
1106	34-35	0.22109995099572188
1106	36-37	0.24534919740860062
1106	38-39	0.18087862242188635
1106	40-41	0.2721942910916795
1106	42-43	0.2979331502753908
1106	44-45	0.2908107884867732
1106	46-47	0.2942475429459961
1106	48-49	0.2907445337198169
1106	50-51	0.3057952419477701
1106	52-53	0.29075199858170464
1106	54-55	0.23373169153962436
1106	56-57	0.2620145105489584
1106	58-59	0.24337155873689653
1106	60-61	0.24446050718262313
1106	62-63	0.24336062408227832
1106	64-65	0.23387384971503877
1106	66-67	0.236350819984672
1106	68-69	0.2230281934509577
1106	70-71	0.2062060264436809
1106	72-73	0.21412095971401612
1106	74-75	0.19792909167414763
1106	76-77	-0.5969891671210803
1106	78-79	0.1667068658656916
1106	80-81	0.16642501627875816
1106	82-83	0.1840354039729135
1106	84-85	0.22394644912986195
1106	86-87	0.1901618216522607
1106	88-89	0.16838963882732116
1106	90-91	0.21040375960012625
1106	92-93	0.18965002444978296
1106	94-95	0.1880172179872801
1106	96-97	0.24024040592221851
1106	98-99	0.3011277764355107
1106	100	0.3304957273106659
1107	1	0.48190433601288163
1107	2	0.3659102449777336
1107	3	0.30561071329269396
1107	4	0.19116211533943073
1107	5	0.22955697761151583
1107	6	0.20070782573574775
1107	7	0.16021052732591556
1107	8	0.21101891973469833
1107	9	0.2504557214744523
1107	10-11	0.2723385487832459
1107	12-13	0.30210272145145467
1107	14-15	0.34198355496853594
1107	16-17	0.3515794543660391
1107	18-19	0.35341585620845706
1107	20-21	0.35751974748436055
1107	22-23	0.3970400656738775
1107	24-25	0.39052524339542316
1107	26-27	0.40997630296545395
1107	28-29	0.3975464735385046
1107	30-31	0.4303272379630485
1107	32-33	0.4125868044227303
1107	34-35	0.4361954265507393
1107	36-37	0.4168598436825306
1107	38-39	0.44704831361202224
1107	40-41	0.486220777667981
1107	42-43	0.39007814759852266
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2314	96-97	-0.32933191437898657
2314	98-99	-0.34020348890353347
2314	100	-0.325251919387032
2315	1	-1.5302903451167715
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2315	14-15	-0.4572094219924949
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2315	100	-0.35323241530863925
2316	1	-4.292871103402305
2316	2	-2.2696756167481418
2316	3	-1.2378544444428314
2316	4	-0.7248685847425662
2316	5	-0.6649605274527914
2316	6	-0.9370313469109988
2316	7	-0.6445142918795597
2316	8	-0.5179829100240312
2316	9	-0.5089424114646164
2316	10-11	-0.4709759373155933
2316	12-13	-0.41473580562233536
2316	14-15	-0.6015810730504754
2316	16-17	-0.5985676732114698
2316	18-19	-0.6184775776596894
2316	20-21	-0.6127316574266928
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2316	30-31	-0.5819038011334428
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2316	34-35	-0.5688182138122855
2316	36-37	-0.5930888047985903
2316	38-39	-0.6310795296611218
2316	40-41	-0.6433367149915199
2316	42-43	-0.6717014785131212
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2316	46-47	-0.6504085347509516
2316	48-49	-0.635490881793622
2316	50-51	-0.6000055811456164
2316	52-53	-0.6129193612035095
2316	54-55	-0.6669002751287465
2316	56-57	-0.5800452612363429
2316	58-59	-0.5209797803036906
2316	60-61	-0.4730795274869166
2316	62-63	-0.5885575662112927
2316	64-65	-0.5380051382652908
2316	66-67	-0.49531462591765063
2316	68-69	-0.45895906938707043
2316	70-71	-0.4707517319221992
2316	72-73	-0.45769789713803277
2316	74-75	-0.5193173888947697
2316	76-77	-0.44628590767561604
2316	78-79	-0.542427911819324
2316	80-81	-0.45241969844528995
2316	82-83	-0.45270240210940216
2316	84-85	-0.4328364962186342
2316	86-87	-0.41951864436109787
2316	88-89	-0.42571521479862895
2316	90-91	-0.48211510539749725
2316	92-93	-0.48598752589284544
2316	94-95	-0.503142326218228
2316	96-97	-0.47079591261121934
2316	98-99	-0.46549039328692743
2316	100	-0.4869874343013407
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	678.0
3	174.0
4	349.0
5	743.0
6	1479.0
7	2738.0
8	5046.0
9	8321.0
10	12309.0
11	16989.0
12	22689.0
13	28460.0
14	30329.0
15	31702.0
16	40613.0
17	55262.0
18	74906.0
19	115411.0
20	234529.0
21	298748.0
22	124538.0
23	98262.0
24	112731.0
25	131262.0
26	156465.0
27	194093.0
28	247322.0
29	311473.0
30	385893.0
31	481529.0
32	607422.0
33	787110.0
34	1092428.0
35	1883530.0
36	3273491.0
37	6221216.0
38	9106534.0
39	3376948.0
40	11240.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.773858965704772	36.41547623938074	20.478098115856252	28.332566679058235
2	20.78462471629753	31.454856390999993	30.78243179959764	16.978087093104847
3	23.19031878425262	28.019299129064283	26.947055061284175	21.843327025398917
4	19.412183121952296	27.809415472630995	27.243763233496804	25.53463817191991
5	21.55651239301913	31.91134739331421	26.574173054540346	19.957967159126316
6	25.091429896039752	26.903374085793992	26.74149792722397	21.263698090942285
7	21.905774291682377	27.25062144747727	30.174431185681428	20.669173075158927
8	23.619242776110376	26.361281113019512	28.402994061645238	21.61648204922487
9	23.81383488003128	25.94564765707659	29.051120633516447	21.18939682937568
10-11	20.49952945689097	29.083354915243763	29.194413026455805	21.22270260140946
12-13	20.7177771598963	27.29158989624526	28.42478384795627	23.56584909590217
14-15	20.889026323576147	31.359146244636044	25.987336066208233	21.76449136557958
16-17	23.50860379675323	26.964334110011702	25.877117908753778	23.649944184481292
18-19	21.455621609383847	29.020549358826287	25.803188457703612	23.720640574086254
20-21	23.508208325567566	28.967319613254872	26.06932197513047	21.455150086047095
22-23	23.10337765517495	26.942098151080945	28.487165202375447	21.467358991368656
24-25	21.045218141504268	28.652081025759564	26.57968695972689	23.72301387300928
26-27	21.06429511607723	26.629584774092073	30.914589759480776	21.391530350349925
28-29	21.145052130349143	26.692463526642303	30.688234082742692	21.474250260265862
30-31	21.20184565067159	26.673202052846317	30.534457586107827	21.590494710374266
32-33	21.230183016930212	26.77238857699951	30.45865377943696	21.538774626633316
34-35	21.30016677847162	26.800627379046215	30.348944895786406	21.550260946695758
36-37	21.242071438995428	26.80621367814671	30.327456826148687	21.62425805670918
38-39	21.23666239625388	26.833879320176244	30.47370147036187	21.45575681320801
40-41	21.156978332437944	26.909034055882852	30.49020985729169	21.443777754387515
42-43	21.116454366241875	26.846179488079112	30.48160751397957	21.555758631699444
44-45	21.125714138148968	26.904660212171304	30.51117321022755	21.458452439452177
46-47	21.097610333249712	27.0095631692885	30.423566202315893	21.469260295145894
48-49	21.17510071501866	26.95517067082932	30.3264915466175	21.543237067534513
50-51	21.167177770922944	27.014126298353876	30.309868236437147	21.508827694286037
52-53	21.228390386601724	27.14099820153445	30.10590325286675	21.524708158997072
54-55	21.359290088409956	27.242866735397993	29.68913540437767	21.708707771814385
56-57	21.52732699607743	27.636617057613922	29.13072368624508	21.705332260063564
58-59	21.552840576423407	27.89612200070876	28.823486860489467	21.72755056237836
60-61	21.49479170867706	27.988310697899895	28.723981942382782	21.79291565104026
62-63	21.408837016478795	28.057121642544192	28.775036645818435	21.759004695158577
64-65	21.41719632193071	28.161593720960447	28.69661268957972	21.724597267529123
66-67	21.35119594913009	28.030829454728913	28.837087257259213	21.780887338881787
68-69	21.308607731184512	27.971962580178406	29.033733421729597	21.68569626690749
70-71	21.326605050227883	27.935754996068614	29.108132706068712	21.629507247634795
72-73	21.27989997071821	27.90015405474752	29.128513946134145	21.69143202840013
74-75	21.293605864656744	27.969463802444988	29.119531502398143	21.617398830500125
76-77	21.327270929061868	28.11047044779033	28.960704766157885	21.601553856989913
78-79	21.337889543958337	28.186599688171153	28.77973328145087	21.69577748641964
80-81	21.36210972155184	28.316399409092437	28.672911499345545	21.648579370010182
82-83	21.411939015210557	28.430971793908462	28.453906682208626	21.703182508672356
84-85	21.355401063930344	28.483189890378885	28.365976279280787	21.79543276640998
86-87	21.356021215454724	28.561552139237197	28.329451030391407	21.752975614916675
88-89	21.42950644913554	28.616116780722628	28.227011735516662	21.727365034625173
90-91	21.460395641208372	28.591800424615236	28.14730120996543	21.800502724210954
92-93	21.465019215156037	28.653496753452746	28.13909039754634	21.742393633844877
94-95	21.530622914618295	28.71263158480027	28.038892327103415	21.71785317347802
96-97	21.536045914137052	28.726967775047335	27.930193048752265	21.806793262063344
98-99	21.524437641709024	28.779059106337296	27.937440305134746	21.75906294681893
100	21.603359682324857	28.82415174747693	27.70660004718617	21.865888523012046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179192.0
1	108201.5
2	30337.0
3	21391.0
4	18375.0
5	16593.0
6	15903.0
7	18377.5
8	17949.5
9	17390.0
10	20641.5
11	35229.0
12	226345.5
13	233231.5
14	58878.0
15	57216.0
16	62342.5
17	69080.0
18	75778.0
19	83483.0
20	95145.5
21	113728.0
22	140023.5
23	170500.0
24	201172.5
25	234523.5
26	270633.5
27	312330.5
28	369014.0
29	423708.0
30	465266.5
31	512578.5
32	561337.5
33	594238.5
34	625218.5
35	671521.0
36	719201.5
37	748396.5
38	777521.0
39	812514.5
40	835425.5
41	865686.5
42	906051.0
43	914872.0
44	909561.0
45	920647.5
46	934366.5
47	939152.0
48	935007.0
49	926842.5
50	915890.5
51	906893.5
52	895496.5
53	874666.5
54	842660.5
55	802361.5
56	755475.0
57	698370.0
58	630862.0
59	554517.5
60	470495.5
61	391011.0
62	321381.5
63	256752.0
64	204393.0
65	166228.5
66	135668.0
67	112024.5
68	93526.0
69	78465.5
70	65928.0
71	54803.5
72	44964.5
73	35711.5
74	27653.5
75	21991.5
76	16184.5
77	10357.0
78	6466.5
79	4108.5
80	2584.0
81	1724.5
82	1114.5
83	681.0
84	424.5
85	242.0
86	133.5
87	82.0
88	50.0
89	32.0
90	20.0
91	15.0
92	13.0
93	7.5
94	3.5
95	1.5
96	0.5
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028382662786587323
2	0.01134022075133982
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007175942967241263
26-27	0.028838975693124096
28-29	0.03351533796122503
30-31	0.03059324531158769
32-33	0.03747173986567906
34-35	0.03009130111439724
36-37	2.3491664447633902E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.005703911331709671
54-55	0.03345956638375942
56-57	0.04612309456405589
58-59	0.0510428237156431
60-61	0.05539131671015836
62-63	0.037138800448687406
64-65	0.0010478296372325915
66-67	2.7040764831808806E-5
68-69	0.0
70-71	0.0
72-73	8.450239009940253E-5
74-75	2.7040764831808806E-5
76-77	0.0
78-79	4.833536713685825E-4
80-81	0.01703399179623756
82-83	0.040941408003160526
84-85	0.05508541805799853
86-87	0.05901646924542272
88-89	0.058271158164746
90-91	0.03682276150971565
92-93	0.002579012945833765
94-95	1.6900478019880504E-6
96-97	0.0
98-99	4.427925241208693E-4
100	7.436210328747423E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.9584962E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.72679654699521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83266682559415	37.438097245737936
2	11.97754411043755	11.672547100039024
3	4.085764748360637	5.972586828967614
4	2.0742844026100866	4.042929362663487
5	1.208361516218461	2.9439792877997797
6	0.802709954789131	2.3468090791954626
7	0.5382907809232724	1.8360429755620027
8	0.4028344908798111	1.5703067423370365
9	0.2959871322657575	1.2980254296997915
>10	1.5768262542560807	14.105302126330347
>50	0.12476078178612815	4.196140569093722
>100	0.0788111074739397	7.133193536260976
>500	7.560840992512317E-4	0.22310233489275613
>1k	3.050550614030162E-4	0.3401957764842941
>5k	5.5288711081410534E-5	0.20763316724303627
>10k+	4.1466533309408834E-5	4.673108437692681
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1061518	3.588032325341503	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	225995	0.763884706020579	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	52391	0.17708658878791192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4955271532882144E-4	1.0140286811928304E-5	0.0	3.380095603976101E-6	4.394124285168931E-5
2	5.644759658640089E-4	3.380095603976101E-5	0.0	3.380095603976101E-6	4.394124285168931E-5
3	7.165802680429334E-4	5.408152966361762E-5	0.0	6.760191207952202E-6	4.394124285168931E-5
4	8.619243790139058E-4	7.098200768349812E-5	0.0	6.760191207952202E-6	6.760191207952202E-5
5	0.001034309254816687	7.774219889145032E-5	0.0	1.6900478019880505E-5	7.436210328747423E-5
6	0.0012100742262234443	9.126258130735473E-5	0.0	1.6900478019880505E-5	8.112229449542643E-5
7	0.0014230202492739386	1.0140286811928303E-4	0.0	2.0280573623856608E-5	9.464267691133083E-5
8	0.001608925507492624	1.0478296372325913E-4	0.0	2.0280573623856608E-5	1.0816305932723524E-4
9	0.0017711700964834769	1.0816305932723524E-4	0.0	2.0280573623856608E-5	1.1492325053518744E-4
10-11	0.0021243900870989794	1.1154315493121133E-4	0.0	2.366066922783271E-5	1.233734895451277E-4
12-13	0.0026364745711013587	1.2506353734711574E-4	0.0	2.873081263379686E-5	1.33513776357056E-4
14-15	0.0031908102501534393	1.402739675650082E-4	0.0	3.0420860435784908E-5	1.3520382415904404E-4
16-17	0.003784017028651245	1.504142543769365E-4	0.0	3.5491003841749065E-5	1.4534411097097234E-4
18-19	0.00449890724889219	1.571744455848887E-4	0.0	4.5631290653677366E-5	1.5886449338687675E-4
20-21	0.005136055270241685	1.67314732396817E-4	0.0	4.7321338455665414E-5	1.740749236047692E-4
22-23	0.0056887009014917785	1.774550192087453E-4	0.0	5.0701434059641516E-5	1.8590525821868557E-4
24-25	0.006133183473414636	1.8759530602067363E-4	0.0	5.408152966361762E-5	1.9266544942663776E-4
26-27	0.006587806332149421	1.9266544942663776E-4	1.6900478019880506E-6	5.408152966361762E-5	2.129460230504944E-4
28-29	0.006956236752982816	2.0449578404055412E-4	3.380095603976101E-6	5.408152966361762E-5	2.416768356842912E-4
30-31	0.007302696552390367	2.3491664447633905E-4	6.760191207952202E-6	5.5771577465605666E-5	2.67027552714112E-4
32-33	0.0076947876424515935	2.501270746942315E-4	6.760191207952202E-6	6.0841720871569817E-5	2.906882219419447E-4
34-35	0.008105469258334691	2.704076483180881E-4	6.760191207952202E-6	6.422181647554593E-5	2.9575836534790885E-4
36-37	0.00848742006158399	2.805479351300164E-4	1.0140286811928304E-5	7.098200768349813E-5	3.244891779817057E-4
38-39	0.008926832490100883	2.940683175459208E-4	1.0140286811928304E-5	8.619243790139057E-5	3.532199906155026E-4
40-41	0.009422016496083381	3.075886999618252E-4	1.0140286811928304E-5	8.957253350536668E-5	3.650503252294189E-4
42-43	0.010000012844363295	3.3124936918965794E-4	1.0140286811928304E-5	9.126258130735473E-5	3.819508032492994E-4
44-45	0.010698002586584359	3.481498472095384E-4	1.0140286811928304E-5	9.295262910934279E-5	4.039214246751441E-4
46-47	0.011598798065043991	3.616702296254428E-4	1.3520382415904405E-5	9.464267691133083E-5	4.1913185489303657E-4
48-49	0.012719299757762068	3.7181051643737115E-4	1.6900478019880505E-5	9.464267691133083E-5	4.275820939029768E-4
50-51	0.014186261249887696	3.785707076453233E-4	1.6900478019880505E-5	9.464267691133083E-5	4.427925241208692E-4
52-53	0.016111225696352087	3.870209466552636E-4	1.6900478019880505E-5	1.0647301152524718E-4	4.816636235665944E-4
54-55	0.018522923909789032	3.9209109006122775E-4	1.6900478019880505E-5	1.1323320273319939E-4	5.070143405964152E-4
56-57	0.02142980612920848	4.258920461009888E-4	1.6900478019880505E-5	1.166132983371755E-4	5.272949142202717E-4
58-59	0.025298325547959126	4.5293281093279755E-4	1.6900478019880505E-5	1.1999339394115158E-4	5.492655356461164E-4
60-61	0.030147072691862845	4.749034323586422E-4	1.6900478019880505E-5	1.2506353734711574E-4	5.779963482799133E-4
62-63	0.03601660870816734	5.019441971904511E-4	1.6900478019880505E-5	1.2844363295109185E-4	6.050371131117221E-4
64-65	0.0439006817044416	5.188446752103315E-4	1.6900478019880505E-5	1.2844363295109185E-4	6.253176867355787E-4
66-67	0.053927735313636704	5.374352010322001E-4	1.6900478019880505E-5	1.2844363295109185E-4	6.726390251912441E-4
68-69	0.06633437622803098	5.458854400421404E-4	1.6900478019880505E-5	1.2844363295109185E-4	6.979897422210649E-4
70-71	0.08206365112113377	5.72926204873949E-4	1.6900478019880505E-5	1.3182372855506793E-4	7.334807460628139E-4
72-73	0.10177974877912638	5.948968262997938E-4	1.6900478019880505E-5	1.368938719610321E-4	7.791120367164914E-4
74-75	0.1261147470799523	6.168674477256385E-4	1.6900478019880505E-5	1.436540631689843E-4	8.078428493502881E-4
76-77	0.15624660934159726	6.439082125574472E-4	1.8590525821868557E-5	1.5210430217892456E-4	8.36573661984085E-4
78-79	0.19366764777321666	6.608086905773277E-4	2.0280573623856608E-5	1.5210430217892456E-4	8.771348092317982E-4
80-81	0.2397704617636487	6.793992163991963E-4	2.0280573623856608E-5	1.537943499809126E-4	9.02485526261619E-4
82-83	0.2977847326624925	7.047499334290171E-4	2.0280573623856608E-5	1.6224458899085286E-4	9.278362432914398E-4
84-85	0.36879547115862443	7.233404592508856E-4	2.0280573623856608E-5	1.6900478019880505E-4	9.633272471331888E-4
86-87	0.4562368543856842	7.65591654300587E-4	2.0280573623856608E-5	1.6900478019880505E-4	0.0010140286811928302
88	0.5336021726172911	7.909423713304077E-4	2.0280573623856608E-5	1.6900478019880505E-4	0.0010444495416286153
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	148115	0.0	77.271416	1
GTGGTAT	145515	0.0	76.27835	6
AGTGGTA	146445	0.0	76.0378	5
TGGTATC	147715	0.0	75.4604	7
GGTATCA	148555	0.0	75.05585	8
GCAGTGG	151085	0.0	74.029144	3
GTATCAA	154345	0.0	73.199234	9
CAGTGGT	155050	0.0	72.06634	4
AGCAGTG	158710	0.0	71.24492	2
CAACGCA	153255	0.0	39.899906	12-13
ATCAACG	154965	0.0	39.37775	10-11
ACGCAGA	156200	0.0	38.959618	14-15
AGAGTAC	157995	0.0	38.16692	18-19
AGTACTT	158675	0.0	37.809036	20-21
GAGTACT	147735	0.0	37.00752	20-21
TCAACGC	153170	0.0	36.931	12-13
AACGCAG	154085	0.0	36.40217	14-15
TATCAAC	157930	0.0	35.969643	10-11
CGCAGAG	157365	0.0	35.45531	16-17
GCAGAGT	170910	0.0	35.288864	16-17
>>END_MODULE