FastQCFastQC Report
Thu 26 May 2016
SRR1660587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27156469
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13826745.091508767211231No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1238740.4561491407443287No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT358460.13199801491129057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2185750.086.209318
AGTGGTA2208400.085.568185
GTGGTAT2209150.085.383216
TGGTATC2209350.085.090227
GTATCAA2212650.085.00749
AAGCAGT2297400.083.255611
GCAGTGG2313850.082.3122253
CAGTGGT2326500.081.3661964
AGCAGTG2393200.079.833372
CAACGCA2155050.045.2708812-13
ATCAACG2176000.044.8692510-11
ACGCAGA2193300.044.62495814-15
AGAGTAC2172500.043.82915518-19
TCAACGC2153350.043.71592712-13
AACGCAG2178850.043.23021714-15
CGCAGAG2206850.042.60249716-17
TATCAAC2243100.041.8806310-11
AGTACTT1860650.041.8740820-21
GAGTACT1777350.041.7082420-21
GCAGAGT2339000.041.49733416-17
Produced by FastQC (version 0.11.5)