FastQCFastQC Report
Thu 26 May 2016
SRR1660587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27156469
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9833983.621229254804813No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1324320.48766281065480194No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT367890.13547048403089518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1244750.080.854988
AGTGGTA1265900.079.945815
GTGGTAT1268900.079.4827656
TGGTATC1274550.078.905547
GTATCAA1276950.078.713099
AAGCAGT1358250.075.458721
GCAGTGG1363400.074.948993
CAGTGGT1379050.073.6248254
AGCAGTG1461950.070.332442
CAACGCA1209450.044.31535312-13
ATCAACG1227350.043.64224610-11
ACGCAGA1246100.043.09491714-15
AGAGTAC1265750.041.97585718-19
TCAACGC1207250.041.6015312-13
AACGCAG1231100.040.70779814-15
AGTACTT1328400.039.72474320-21
GAGTACT1231950.039.5900120-21
CGCAGAG1262300.039.5694916-17
TATCAAC1295950.038.81941610-11
GCAGAGT1398050.038.16570316-17
Produced by FastQC (version 0.11.5)