FastQCFastQC Report
Thu 26 May 2016
SRR1660586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30622697
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13898224.538535583590172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA736860.2406254419720118No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536630.175239300444373No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT373850.12208265000303532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1802650.083.206218
AGTGGTA1837250.081.89635
GTGGTAT1836400.081.8425456
GTATCAA1835100.081.5718159
TGGTATC1838050.081.504577
AAGCAGT1919750.079.782761
GCAGTGG1955200.077.8465963
CAGTGGT1956200.077.188444
AGCAGTG2023550.075.691592
CAACGCA1770700.044.69885612-13
ATCAACG1793500.044.1734210-11
ACGCAGA1813650.043.64935314-15
AGAGTAC1826500.042.7861418-19
TCAACGC1767800.042.40274412-13
AACGCAG1798950.041.59012214-15
AGTACTT1844000.041.5563720-21
GAGTACT1760550.040.93445620-21
CGCAGAG1838400.040.56723416-17
TATCAAC1861350.040.19052510-11
GCAGAGT1967500.039.9102516-17
Produced by FastQC (version 0.11.5)