##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660586_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30622697 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.907954612880765 34.0 31.0 34.0 30.0 34.0 2 32.061692084142685 34.0 31.0 34.0 30.0 34.0 3 32.073944205502215 34.0 31.0 34.0 30.0 34.0 4 35.45467193826853 37.0 35.0 37.0 33.0 37.0 5 35.431155263692155 37.0 35.0 37.0 33.0 37.0 6 35.45927114127146 37.0 35.0 37.0 33.0 37.0 7 35.42997052153832 37.0 35.0 37.0 33.0 37.0 8 35.441047240221856 37.0 35.0 37.0 33.0 37.0 9 37.09591281264351 39.0 37.0 39.0 34.0 39.0 10-11 37.03153525308369 39.0 37.0 39.0 34.0 39.0 12-13 37.05638801180706 39.0 37.0 39.0 34.0 39.0 14-15 38.450058023955236 41.0 38.0 41.0 34.0 41.0 16-17 38.42096071747044 40.5 38.0 41.0 34.0 41.0 18-19 38.36851550012071 40.0 38.0 41.0 34.0 41.0 20-21 38.31953583317629 40.0 38.0 41.0 34.0 41.0 22-23 38.176624400522265 40.0 38.0 41.0 33.5 41.0 24-25 38.16730005851542 40.0 38.0 41.0 34.0 41.0 26-27 38.14673861025369 40.0 38.0 41.0 34.0 41.0 28-29 38.040395854094754 40.0 38.0 41.0 33.0 41.0 30-31 37.9501654606059 40.0 38.0 41.0 33.0 41.0 32-33 37.867402518465305 40.0 38.0 41.0 33.0 41.0 34-35 37.759657387460024 40.0 38.0 41.0 33.0 41.0 36-37 37.58705854680272 40.0 38.0 41.0 32.5 41.0 38-39 37.21466931864296 40.0 36.0 41.0 32.0 41.0 40-41 37.09705440053173 40.0 36.0 41.0 31.5 41.0 42-43 36.94562529877757 40.0 36.0 41.0 31.0 41.0 44-45 36.81347175266764 40.0 35.5 41.0 31.0 41.0 46-47 36.654077317226495 40.0 35.0 41.0 31.0 41.0 48-49 36.489711748772486 39.0 35.0 41.0 30.5 41.0 50-51 35.31092253239484 38.0 34.0 40.0 28.5 40.5 52-53 35.329609358052295 38.5 35.0 39.5 28.5 40.5 54-55 35.65506743249949 39.0 35.0 40.5 28.0 41.0 56-57 35.56604766066164 39.0 35.0 41.0 27.5 41.0 58-59 35.418168621790564 39.0 35.0 41.0 27.0 41.0 60-61 35.205887515394224 39.0 35.0 41.0 26.5 41.0 62-63 34.93629756059697 38.5 35.0 41.0 26.0 41.0 64-65 34.64455545832557 38.0 35.0 40.5 26.0 41.0 66-67 34.33590305582817 37.0 35.0 40.0 26.0 41.0 68-69 34.00809892740669 37.0 34.5 40.0 26.0 41.0 70-71 33.67174209051541 36.0 34.0 39.0 25.0 41.0 72-73 33.31499216087988 36.0 34.0 39.0 24.5 41.0 74-75 32.957227003878856 35.0 34.0 38.5 24.0 40.5 76-77 32.60303334810778 35.0 34.0 37.0 23.0 39.5 78-79 32.25833510026893 35.0 34.0 37.0 21.5 39.0 80-81 31.935248747032308 35.0 33.0 36.0 20.0 39.0 82-83 31.547674246327816 35.0 33.0 36.0 18.5 37.5 84-85 31.288420644334494 35.0 33.0 35.5 17.5 37.0 86-87 30.997020331030935 35.0 33.0 35.0 11.0 37.0 88-89 30.80870744337117 35.0 33.0 35.0 7.5 36.0 90-91 30.619703858873045 35.0 33.0 35.0 3.5 36.0 92-93 30.442985834983773 35.0 33.0 35.0 2.0 36.0 94-95 30.224138063345627 35.0 32.0 35.0 2.0 36.0 96-97 30.0726460180826 35.0 32.0 35.0 2.0 35.0 98-99 29.908545710392524 35.0 32.0 35.0 2.0 35.0 100 29.782033470141446 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.411600408767395 1101 2 0.3673040704195145 1101 3 0.4265119541289977 1101 4 0.3364313851949987 1101 5 0.33706075077060405 1101 6 0.3683882677277879 1101 7 0.32799762636341256 1101 8 0.3646642047557265 1101 9 0.4559605871726262 1101 10-11 0.49660375433128223 1101 12-13 0.45438155820266246 1101 14-15 0.552254118746653 1101 16-17 0.5589287049701994 1101 18-19 0.5112113339416524 1101 20-21 0.5120206236085068 1101 22-23 0.5451854901174613 1101 24-25 0.5558043880963481 1101 26-27 0.5082842810905035 1101 28-29 0.5032159085593619 1101 30-31 0.5509131974306243 1101 32-33 0.5611146148252075 1101 34-35 0.5006422682363905 1101 36-37 0.5380239854874915 1101 38-39 0.5697479078498731 1101 40-41 0.5677126782361341 1101 42-43 0.6248230354486495 1101 44-45 0.5766870226522016 1101 46-47 0.5657777036524223 1101 48-49 0.5596733975454242 1101 50-51 0.5345633438226898 1101 52-53 0.5759227664230124 1101 54-55 0.6580479817485312 1101 56-57 0.7034321051479822 1101 58-59 0.6560492314162687 1101 60-61 0.6520054977777434 1101 62-63 0.6365262273233583 1101 64-65 0.6219519593613683 1101 66-67 0.6116478323828431 1101 68-69 0.544837438347848 1101 70-71 0.514286854974678 1101 72-73 0.5172598720972559 1101 74-75 0.49018120982957925 1101 76-77 0.5528587584510873 1101 78-79 0.5155087926382578 1101 80-81 0.5635650122587919 1101 82-83 0.45279213237621363 1101 84-85 0.41988117600211083 1101 86-87 0.36107527229591696 1101 88-89 0.3631770956716487 1101 90-91 0.259687354902848 1101 92-93 0.2990019795371417 1101 94-95 0.3075526151681949 1101 96-97 0.25368658077773887 1101 98-99 0.2936866868899095 1101 100 0.3043065649108101 1102 1 0.3492858731823141 1102 2 0.3925180211711137 1102 3 0.37707880080471057 1102 4 0.30160899406180164 1102 5 0.2616936077797618 1102 6 0.2686197625617055 1102 7 0.3299373861258914 1102 8 0.35787024820223934 1102 9 0.4427483941784729 1102 10-11 0.3591090042675731 1102 12-13 0.41282978266451664 1102 14-15 0.48944163007738695 1102 16-17 0.5011186723504224 1102 18-19 0.43787636071086666 1102 20-21 0.43988529082064787 1102 22-23 0.4695004749590552 1102 24-25 0.5093530372257362 1102 26-27 0.5197107797993397 1102 28-29 0.5583388551722166 1102 30-31 0.5634011304078186 1102 32-33 0.5072968782400338 1102 34-35 0.4506125971300676 1102 36-37 0.519709310021625 1102 38-39 0.5338356086542575 1102 40-41 0.4402647895912253 1102 42-43 0.48398182880414 1102 44-45 0.4682470831030443 1102 46-47 0.4563551517620894 1102 48-49 0.4943277513945077 1102 50-51 0.5061533544363215 1102 52-53 0.5566052870226841 1102 54-55 0.635340545229873 1102 56-57 0.6603731168678948 1102 58-59 0.6559430597854785 1102 60-61 0.6125694069027432 1102 62-63 0.5776858024166245 1102 64-65 0.6394225608707131 1102 66-67 0.6049269362845138 1102 68-69 0.5531475569761355 1102 70-71 0.47285260994026856 1102 72-73 0.48810525211399636 1102 74-75 0.4478951455978333 1102 76-77 0.4891815581213308 1102 78-79 0.5118184986209471 1102 80-81 0.4855545115997586 1102 82-83 0.41857952437708335 1102 84-85 0.3020917924763644 1102 86-87 0.2997115490892348 1102 88-89 0.30901611556023667 1102 90-91 0.32819162198543594 1102 92-93 0.31602267096422665 1102 94-95 0.3764939527523019 1102 96-97 0.35384665044368546 1102 98-99 0.3265721603517875 1102 100 0.3260994948673357 1103 1 0.3721626396079465 1103 2 0.3882123231172727 1103 3 0.3934489362630771 1103 4 0.31863552630677816 1103 5 0.3094852344781387 1103 6 0.3297471076306664 1103 7 0.2928582106865605 1103 8 0.299760413418646 1103 9 0.40102778992915233 1103 10-11 0.40346929930553443 1103 12-13 0.4237344506259504 1103 14-15 0.494832667856862 1103 16-17 0.4760926945472903 1103 18-19 0.48124817360615424 1103 20-21 0.4839460171613723 1103 22-23 0.4715566369178319 1103 24-25 0.46986991935776956 1103 26-27 0.4194313481268779 1103 28-29 0.48083891251427957 1103 30-31 0.5089020773360176 1103 32-33 0.45708494614951434 1103 34-35 0.46989841194201887 1103 36-37 0.43560845332883247 1103 38-39 0.4473174241197242 1103 40-41 0.4779873019323446 1103 42-43 0.5343384362576771 1103 44-45 0.4718406802799535 1103 46-47 0.44519906616243077 1103 48-49 0.47793133986125014 1103 50-51 0.5631163104386161 1103 52-53 0.526777964638903 1103 54-55 0.5296130024019661 1103 56-57 0.4959808504568244 1103 58-59 0.45686229508604725 1103 60-61 0.47991138949164736 1103 62-63 0.4655261113018412 1103 64-65 0.4746546309419273 1103 66-67 0.479142891718638 1103 68-69 0.47699008651196806 1103 70-71 0.4664056139465558 1103 72-73 0.4094084788759318 1103 74-75 0.44536930316021284 1103 76-77 0.4725265466590898 1103 78-79 0.4381869571435999 1103 80-81 0.38455086603948274 1103 82-83 0.28409665481486 1103 84-85 0.2672839079807865 1103 86-87 0.2927231517595992 1103 88-89 0.2535377984800924 1103 90-91 0.28032264873280965 1103 92-93 0.24858492131212628 1103 94-95 0.21691934479776975 1103 96-97 0.21231466994287018 1103 98-99 0.1788921014979259 1103 100 0.15551800952837525 1104 1 0.26398230671192024 1104 2 0.30676020504660784 1104 3 0.24687275971918865 1104 4 0.2747497444380542 1104 5 0.21333532724544568 1104 6 0.30026152236773385 1104 7 0.27476380252049637 1104 8 0.28518985236858185 1104 9 0.36589714854805777 1104 10-11 0.36755702188532524 1104 12-13 0.3389559611322497 1104 14-15 0.41798997290423046 1104 16-17 0.40870165775402256 1104 18-19 0.39709585166720984 1104 20-21 0.3623289555945277 1104 22-23 0.3248516997482298 1104 24-25 0.3844741577656947 1104 26-27 0.38622507676039675 1104 28-29 0.4568981958976437 1104 30-31 0.43324094802217417 1104 32-33 0.408603295559125 1104 34-35 0.40499957711590184 1104 36-37 0.4172834251761941 1104 38-39 0.40211839156224727 1104 40-41 0.408811234147322 1104 42-43 0.35545437233930244 1104 44-45 0.38843255524133014 1104 46-47 0.3889708699003833 1104 48-49 0.3935845022648792 1104 50-51 0.33726641656899403 1104 52-53 0.4448201213314462 1104 54-55 0.48013725877527946 1104 56-57 0.44136721329439155 1104 58-59 0.4359377319034863 1104 60-61 0.4678012485542453 1104 62-63 0.38430244031464156 1104 64-65 0.42131469996502346 1104 66-67 0.42083812091715345 1104 68-69 0.43050137119042375 1104 70-71 0.3798771110316963 1104 72-73 0.3906543167902612 1104 74-75 0.36586237113515807 1104 76-77 0.43311878283690675 1104 78-79 0.389243715993004 1104 80-81 0.4036044196658679 1104 82-83 0.30116421798929593 1104 84-85 0.2928365036399576 1104 86-87 0.2538417063276093 1104 88-89 0.26657979864256376 1104 90-91 0.2659358407466641 1104 92-93 0.2320217031297389 1104 94-95 0.20145212751758024 1104 96-97 0.22369111440061928 1104 98-99 0.21053979963539504 1104 100 0.1998208549424625 1105 1 0.259695996798019 1105 2 0.2446110617331172 1105 3 0.1868679303453007 1105 4 0.23212724018041797 1105 5 0.2231573150306474 1105 6 0.2008920222784738 1105 7 0.23745050041290483 1105 8 0.16970139855946087 1105 9 0.3008926073139122 1105 10-11 0.3299151431749223 1105 12-13 0.32929983126404494 1105 14-15 0.3884697806141446 1105 16-17 0.3943214956678318 1105 18-19 0.40279000019567235 1105 20-21 0.2987315793125802 1105 22-23 0.3571202343232187 1105 24-25 0.3625304984527915 1105 26-27 0.3972871600512917 1105 28-29 0.38495669295375734 1105 30-31 0.37203228164649005 1105 32-33 0.3171608442432827 1105 34-35 0.3520269514940111 1105 36-37 0.3503018638062656 1105 38-39 0.40348888058785093 1105 40-41 0.40277535518278285 1105 42-43 0.40523936700390806 1105 44-45 0.3956774202080595 1105 46-47 0.37131078865585465 1105 48-49 0.3747721714408456 1105 50-51 0.47436023428353735 1105 52-53 0.4469550866559544 1105 54-55 0.3900481408257548 1105 56-57 0.40494314432325496 1105 58-59 0.3655729395545606 1105 60-61 0.35922574018503184 1105 62-63 0.33337278229988954 1105 64-65 0.2943127258343736 1105 66-67 0.3019344776102315 1105 68-69 0.29100191994302804 1105 70-71 0.32739915137188547 1105 72-73 0.27706823294339245 1105 74-75 0.2761961342532686 1105 76-77 0.24629530759930418 1105 78-79 0.2546111588461244 1105 80-81 0.23416249078847073 1105 82-83 0.2387313500525039 1105 84-85 0.18722354565504062 1105 86-87 0.1845963808214357 1105 88-89 0.18370777848986464 1105 90-91 0.19973029118414587 1105 92-93 0.1925962965272987 1105 94-95 0.2611492219024747 1105 96-97 0.15306438370459574 1105 98-99 0.18912082588385104 1105 100 0.19133523733926694 1106 1 0.21563747774515107 1106 2 0.24857079818581695 1106 3 0.2458351180216809 1106 4 0.1600157017213064 1106 5 0.127036160819209 1106 6 0.22692610441295358 1106 7 0.2180481703287569 1106 8 0.2409676648143062 1106 9 0.2975857421217327 1106 10-11 0.29098052390150286 1106 12-13 0.3065076062686174 1106 14-15 0.2973870401793093 1106 16-17 0.27996339149879645 1106 18-19 0.31068400731911794 1106 20-21 0.36732263267125376 1106 22-23 0.3674235116939286 1106 24-25 0.3208206368471096 1106 26-27 0.3617054076334796 1106 28-29 0.36798532065397893 1106 30-31 0.3282234898146683 1106 32-33 0.30540926508665933 1106 34-35 0.3580236561907384 1106 36-37 0.3566495181125191 1106 38-39 0.3310836181720518 1106 40-41 0.3552824378708408 1106 42-43 0.3813478919268434 1106 44-45 0.38037596488454284 1106 46-47 0.3754244805295528 1106 48-49 0.3488961668402837 1106 50-51 0.4015114477566897 1106 52-53 0.4038528983820342 1106 54-55 0.4586074619998044 1106 56-57 0.4253323479084443 1106 58-59 0.4094301433836449 1106 60-61 0.4137105805450503 1106 62-63 0.4013052724272512 1106 64-65 0.35900975109316846 1106 66-67 0.3179823372455246 1106 68-69 0.4139895712466952 1106 70-71 0.3917396374197537 1106 72-73 0.27406061791008085 1106 74-75 0.33645974825446245 1106 76-77 0.33100128010573826 1106 78-79 0.34032134832234817 1106 80-81 0.3037027841008353 1106 82-83 0.2824833920164913 1106 84-85 0.28132903608866044 1106 86-87 0.24783209407536688 1106 88-89 0.19828907794406447 1106 90-91 0.20995943217659985 1106 92-93 0.22919081724892365 1106 94-95 0.28197121302719097 1106 96-97 0.16916668698820558 1106 98-99 0.13622881136896225 1106 100 0.09746254420702627 1107 1 0.3962816853286988 1107 2 0.3875868409388943 1107 3 0.44619547219551947 1107 4 0.3761107226830305 1107 5 0.3376850749981344 1107 6 0.4181343669266937 1107 7 0.40162959944552057 1107 8 0.42901089356880107 1107 9 0.5040143513279602 1107 10-11 0.49409629141943157 1107 12-13 0.4754502089713597 1107 14-15 0.582996734520016 1107 16-17 0.5590134123684152 1107 18-19 0.5285980179024463 1107 20-21 0.5871254207304233 1107 22-23 0.5993574593219861 1107 24-25 0.579526695314506 1107 26-27 0.6411182624432072 1107 28-29 0.6558624720608961 1107 30-31 0.6746592466697692 1107 32-33 0.6599028611170965 1107 34-35 0.6661452328769712 1107 36-37 0.6500888545759551 1107 38-39 0.6197743121630523 1107 40-41 0.7088448711801334 1107 42-43 0.6906894865179183 1107 44-45 0.6631037363777637 1107 46-47 0.6960293701888531 1107 48-49 0.7390294826347343 1107 50-51 0.7615579367518137 1107 52-53 0.7470812918317193 1107 54-55 0.787010256455531 1107 56-57 0.7487023968689286 1107 58-59 0.7469799743355878 1107 60-61 0.6684764353774639 1107 62-63 0.6840400014828987 1107 64-65 0.6630259299802503 1107 66-67 0.704944307357593 1107 68-69 0.6526903300510938 1107 70-71 0.6632651056626813 1107 72-73 0.6672262035694558 1107 74-75 0.7010630353577696 1107 76-77 0.6730594657630817 1107 78-79 0.6347481242402893 1107 80-81 0.6175186679340783 1107 82-83 0.5735647757978732 1107 84-85 0.6059619772384401 1107 86-87 0.585021322058612 1107 88-89 0.5320789357889701 1107 90-91 0.5377559585523173 1107 92-93 0.5644290242931156 1107 94-95 0.583600005751471 1107 96-97 0.5479715403362384 1107 98-99 0.5324056957619696 1107 100 0.5392388209923489 1108 1 0.11646254984880855 1108 2 0.16254307147037395 1108 3 0.1208734514913985 1108 4 0.09530050500058707 1108 5 0.09907599643909037 1108 6 0.17354539780728828 1108 7 0.20355311471471538 1108 8 0.15863328012513733 1108 9 0.08538938072960178 1108 10-11 0.11943107379411799 1108 12-13 0.16559799551626497 1108 14-15 0.19154436263226415 1108 16-17 0.20557703483097356 1108 18-19 0.19456115614836023 1108 20-21 0.1542882643428385 1108 22-23 0.19800412640677933 1108 24-25 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-0.8880009151438415 2316 80-81 -0.8922839398042584 2316 82-83 -0.853814273069279 2316 84-85 -0.8505880283235001 2316 86-87 -0.8169843330275484 2316 88-89 -0.8040348287706074 2316 90-91 -0.8101280485445947 2316 92-93 -0.8188183410927863 2316 94-95 -0.7447715467378337 2316 96-97 -0.8399293597487052 2316 98-99 -0.8005401011835893 2316 100 -0.7257053279698162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 297369.0 3 50111.0 4 33156.0 5 31172.0 6 33098.0 7 34655.0 8 39877.0 9 48166.0 10 56084.0 11 61951.0 12 61576.0 13 60298.0 14 58837.0 15 57205.0 16 67509.0 17 86378.0 18 116280.0 19 181116.0 20 354573.0 21 443763.0 22 192377.0 23 154905.0 24 173770.0 25 198590.0 26 216090.0 27 246398.0 28 290746.0 29 348089.0 30 420878.0 31 521442.0 32 657318.0 33 851799.0 34 1179912.0 35 1929824.0 36 3376910.0 37 6575625.0 38 8381228.0 39 2724071.0 40 9551.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.80671599180566 33.88623591620016 26.978048985945506 22.328999106048677 2 21.742577480240254 32.122893475495026 26.94946314337156 19.185065900893164 3 25.581386990246923 25.726817874618575 26.36928655514036 22.322508579994143 4 20.63079814129866 27.0243840102295 25.54527020257312 26.799547645898713 5 22.07303820135485 31.220673949193912 25.743631621733005 20.962656227718238 6 27.053525257506926 26.058176816246263 25.99849827810009 20.889799648146713 7 21.677345208551987 26.11818654425252 31.018522333061956 21.185945914133537 8 26.498147622026398 25.98366668309533 26.0332470114525 21.484938683425778 9 26.25700289683932 25.523270787572223 26.63544479831801 21.584281517270444 10-11 21.164843317100537 28.88113806298701 28.656107492526377 21.297911127386072 12-13 21.21056722924191 26.30453945747953 28.434410406967604 24.05048290631095 14-15 21.23293212986246 31.554467334891438 25.816377528768363 21.396223006477737 16-17 24.04174032654414 26.101695120326944 25.83310232954823 24.023462223580687 18-19 21.434302315533174 28.792718194805435 25.793889134525394 23.979090355135995 20-21 24.102466180587157 28.769076736459958 25.7673820149687 21.36107506798419 22-23 23.975255109925776 26.177321043639466 28.48082748555657 21.36659636087819 24-25 21.451171732897755 28.509275077101044 25.804918823032207 24.23463436696899 26-27 21.496843785384108 25.912363791231574 31.12923616965454 21.46155625372978 28-29 21.618521109152436 25.93953493149278 31.039547793074295 21.402396166280493 30-31 21.552315665863116 25.916078096421234 31.030711799774373 21.500894437941266 32-33 21.579510130879733 25.954060982251715 30.958210177314417 21.50821870955414 34-35 21.55358087277614 26.041252286186623 30.924238691986083 21.480928149051152 36-37 21.568888094093587 25.990989676912875 30.89591468747484 21.5442075415187 38-39 21.614647036523767 25.94756499507751 30.92099393786541 21.51679403053332 40-41 21.570007957855903 26.022002400600787 30.93172807121815 21.47626157032516 42-43 21.5816412425735 26.009837223363043 30.8662512920909 21.542270241972556 44-45 21.618136661874335 26.00295015322782 30.862591586509097 21.51632159838875 46-47 21.601891954337617 26.07454958081661 30.833786683554678 21.489771781291097 48-49 21.650280763041852 26.042856637621504 30.760482155690372 21.54638044364627 50-51 21.662324669827534 26.050187323470887 30.738737802456395 21.548750204245184 52-53 21.683661024801097 26.199477872168742 30.550345799260548 21.566515303769616 54-55 21.93013762493463 26.433577737239222 29.89062292994666 21.745661707879485 56-57 22.265806531542353 26.85560993232751 28.956782867349585 21.92180066878055 58-59 22.29364830129064 27.235630860278615 28.55043263047874 21.92028820795201 60-61 22.272641554296186 27.386335412908657 28.37021010803513 21.970812924760025 62-63 22.20769873923079 27.486326465165018 28.342629110996707 21.963345684607486 64-65 22.18768703298066 27.51560125475226 28.422153515215225 21.874558197051858 66-67 22.14222195968857 27.314909031107554 28.634655789923535 21.90821321928034 68-69 22.092124339276378 27.11609478522198 28.938240820252304 21.85354005524934 70-71 22.04622523006002 27.035300669863094 29.16648434538812 21.751989754688765 72-73 22.03273349190851 26.96784830798009 29.223960971936624 21.775457228174773 74-75 22.078176813093357 27.03377200558314 29.140441428596915 21.74760975272659 76-77 22.1635976161481 27.18335818576147 28.891760536135703 21.761283661954735 78-79 22.283542869970297 27.295736404835814 28.58142620977584 21.839294515418054 80-81 22.33165086368311 27.45182603052777 28.34802590848412 21.868497197305004 82-83 22.34549019492391 27.634040651754855 28.15040141784256 21.870067735478678 84-85 22.36654906612202 27.71315257304641 28.000491099900586 21.91980726093098 86-87 22.410723303463275 27.751118503511385 27.92232895419991 21.915829238825427 88-89 22.44760811774067 27.833350490121695 27.82528609175995 21.893755300377684 90-91 22.52021089843661 27.80256463996058 27.7212039955568 21.956020466046006 92-93 22.549698286852742 27.811660444158303 27.69705278248914 21.941588486499818 94-95 22.621782439072362 27.860689245357 27.650411461377484 21.867116854193153 96-97 22.630833467602447 27.831299229290117 27.602028547722423 21.935838755385014 98-99 22.668513293466006 27.80932407327523 27.604381514970296 21.91778111828847 100 22.66522269528323 27.88331979146062 27.555608874199617 21.895848639056535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 126071.0 1 73843.5 2 16393.0 3 10094.5 4 8907.5 5 8872.0 6 9287.0 7 9744.5 8 10162.5 9 10836.5 10 12126.5 11 19654.5 12 171347.5 13 196133.5 14 71175.5 15 69392.0 16 75978.0 17 83130.0 18 89670.0 19 96347.5 20 104567.5 21 117046.0 22 135616.0 23 158695.5 24 184591.0 25 214052.0 26 247752.5 27 288186.5 28 335387.5 29 380235.5 30 421363.0 31 462241.0 32 504316.0 33 545747.5 34 586329.5 35 632782.0 36 682805.0 37 726973.0 38 772021.0 39 810782.5 40 837078.0 41 870749.0 42 909503.5 43 938147.5 44 964675.5 45 996542.0 46 1022804.0 47 1036023.0 48 1043031.0 49 1047359.5 50 1043296.5 51 1036129.5 52 1030705.0 53 1013500.5 54 976246.0 55 929617.0 56 877425.5 57 811105.5 58 731845.0 59 644998.5 60 545356.5 61 446412.0 62 358837.0 63 277019.0 64 207747.5 65 152562.0 66 109540.0 67 79664.0 68 58006.0 69 43367.0 70 33993.0 71 27484.5 72 23211.5 73 18729.0 74 14738.0 75 12425.0 76 10016.5 77 7110.5 78 4948.5 79 3516.5 80 2424.0 81 1720.5 82 1188.5 83 826.5 84 637.0 85 507.5 86 400.5 87 324.0 88 267.5 89 229.5 90 217.5 91 193.5 92 149.5 93 128.5 94 101.5 95 74.0 96 65.5 97 58.0 98 41.0 99 26.5 100 19.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022649866535269573 2 0.03830818689810372 3 0.05806477463431781 4 0.13501423470310273 5 0.1808397215960436 6 0.11041156825605532 7 0.1240811676385003 8 0.15039171762043035 9 0.13643801524078691 10-11 0.1348558554460438 12-13 0.13537181261336975 14-15 0.14812869029791856 16-17 0.13842673622117607 18-19 0.14176249727448892 20-21 0.15115095838880555 22-23 0.1603777746943713 24-25 0.15689506381492133 26-27 0.1480094976611629 28-29 0.16663783728781303 30-31 0.15918911387850654 32-33 0.1550794170742048 34-35 0.14258868185254878 36-37 0.15018598786383838 38-39 0.14593423956093743 40-41 0.13993542110285062 42-43 0.14483701419244688 44-45 0.1379744573118429 46-47 0.14627059138520687 48-49 0.14011339367006112 50-51 0.1425103086119423 52-53 0.15799882028679577 54-55 0.1602030676788527 56-57 0.15461081040641195 58-59 0.15256330949556793 60-61 0.1531641710068842 62-63 0.14699880941250865 64-65 0.146518773313794 66-67 0.1594862790824727 68-69 0.15390218568926178 70-71 0.1463195746605859 72-73 0.15032150825905372 74-75 0.15020394839814405 76-77 0.11475965033386837 78-79 0.031848925651453884 80-81 0.040290376775109 82-83 0.1708112123501075 84-85 0.04523932036423833 86-87 0.18931709378831002 88-89 0.04196233924138034 90-91 0.17990740658799584 92-93 0.18935791318445924 94-95 0.24727737076848588 96-97 0.2739912163843701 98-99 0.30185127064412387 100 0.31879295282188896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0622697E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.45031021829677 #Duplication Level Percentage of deduplicated Percentage of total 1 75.80781534685245 36.7291217052636 2 12.011391634767547 11.639113017158849 3 4.437282828226537 6.449631886616909 4 2.1645158922057774 4.194858657992134 5 1.3206948869761557 3.199403848885657 6 0.8390797637894316 2.4392204912095883 7 0.5953913761136495 2.019282781180345 8 0.42359221842113465 1.6418539510848398 9 0.3264372732109715 1.4234388438497818 >10 1.8801736679497325 17.134582139295176 >50 0.16276995232499858 5.375455965984823 >100 0.030049940907971896 2.0137821585003652 >500 2.2438481779772586E-4 0.07441134330424332 >1k 5.270774258224038E-4 0.5110032201539741 >5k 2.6878004917999282E-5 0.09379764450027603 >10k+ 2.6878004908561658E-5 5.061042345019418 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1389822 4.538535583590172 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73686 0.2406254419720118 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53663 0.175239300444373 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 37385 0.12208265000303532 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017111490865745758 2.285886184355349E-5 0.0 0.0 0.0 2 0.0017372735001100655 2.6124413535489705E-5 0.0 0.0 3.265551691936213E-6 3 0.0017764601204132999 3.265551691936213E-5 0.0 3.265551691936213E-6 3.265551691936213E-6 4 0.001792787878872981 3.592106861129835E-5 0.0 3.265551691936213E-6 9.79665507580864E-6 5 0.0018319744991762157 3.592106861129835E-5 0.0 9.79665507580864E-6 1.3062206767744853E-5 6 0.0018613644644036415 5.224882707097941E-5 0.0 9.79665507580864E-6 1.6327758459681065E-5 7 0.0018809577745552588 7.510768891453291E-5 0.0 9.79665507580864E-6 1.959331015161728E-5 8 0.0019070821880907485 8.163879229840533E-5 0.0 9.79665507580864E-6 2.285886184355349E-5 9 0.0019495343600859194 9.79665507580864E-5 0.0 9.79665507580864E-6 2.285886184355349E-5 10-11 0.001988720980389154 1.2082541260163989E-4 0.0 1.3062206767744853E-5 2.4491637689521596E-5 12-13 0.0020736253243794954 1.4041872275325716E-4 0.0 2.1226085997585385E-5 2.6124413535489705E-5 14-15 0.0021568968925238688 1.6164480875084257E-4 0.0 2.4491637689521596E-5 2.7757189381457814E-5 16-17 0.002240168460668242 1.730742396726193E-4 0.0 2.6124413535489705E-5 2.938996522742592E-5 18-19 0.0023201744771206793 1.910347739782685E-4 0.0 3.1022741073394025E-5 3.265551691936214E-5 20-21 0.002375688855883595 2.0736253243794952E-4 0.0 3.7553844457266454E-5 4.4084947841138876E-5 22-23 0.00242303935541667 2.2858861843553493E-4 0.0 4.245217199517077E-5 4.571772368710698E-5 24-25 0.0025193731303287887 2.547130319710246E-4 1.6327758459681066E-6 4.571772368710698E-5 5.0616051225011305E-5 26-27 0.002648362422160269 2.775718938145781E-4 3.265551691936213E-6 5.0616051225011305E-5 5.3881602916947516E-5 28-29 0.0028491938512143463 2.857357730444187E-4 3.265551691936213E-6 5.3881602916947516E-5 5.551437876291562E-5 30-31 0.0033880098803838214 3.134929624258765E-4 3.265551691936213E-6 6.367825799275616E-5 6.694380968469237E-5 32-33 0.004452579731955026 3.445157034992705E-4 3.265551691936213E-6 6.531103383872426E-5 7.837324060646912E-5 34-35 0.006367825799275616 3.5921068611298343E-4 3.265551691936213E-6 6.531103383872426E-5 8.980267152824586E-5 36-37 0.009300291218634335 3.8696787549444126E-4 3.265551691936213E-6 6.531103383872426E-5 9.143544737421396E-5 38-39 0.01372021543367 4.065611856460586E-4 3.265551691936213E-6 6.531103383872426E-5 9.470099906615019E-5 40-41 0.020034159630028667 4.2942004748961203E-4 3.265551691936213E-6 6.531103383872426E-5 1.0613042998792693E-4 42-43 0.028245389359402275 4.5717723687106987E-4 3.265551691936213E-6 6.531103383872426E-5 1.1429430921776747E-4 44-45 0.038879658444192555 4.75137771176719E-4 3.265551691936213E-6 6.694380968469237E-5 1.1919263675567178E-4 46-47 0.052650489929087566 4.914655296364E-4 3.265551691936213E-6 7.510768891453291E-5 1.273565159855123E-4 48-49 0.06954155605562762 5.094260639420492E-4 3.265551691936213E-6 7.510768891453291E-5 1.3715317106132097E-4 50-51 0.08937651703244819 5.241210465557622E-4 3.265551691936213E-6 7.837324060646912E-5 1.436842744451934E-4 52-53 0.1127105166471784 5.339177016315708E-4 3.265551691936213E-6 7.837324060646912E-5 1.4858260198309772E-4 54-55 0.13960886593365698 5.518782359372201E-4 3.265551691936213E-6 8.000601645243722E-5 1.5348092952100203E-4 56-57 0.1705091488186034 5.877993045485183E-4 3.265551691936213E-6 8.490434399034154E-5 1.5348092952100203E-4 58-59 0.20567587498906448 6.204548214678806E-4 3.265551691936213E-6 8.490434399034154E-5 1.6164480875084257E-4 60-61 0.2453425313910137 6.43313683311434E-4 3.265551691936213E-6 8.653711983630965E-5 1.6980868798068308E-4 62-63 0.2889392792542081 6.710708726928919E-4 3.265551691936213E-6 8.816989568227775E-5 1.6980868798068308E-4 64-65 0.3367143005072349 6.906641828445091E-4 3.265551691936213E-6 8.980267152824586E-5 1.730742396726193E-4 66-67 0.38895986202652233 7.216869239179032E-4 3.265551691936213E-6 9.143544737421396E-5 1.779725672105236E-4 68-69 0.44685646074870544 7.396474582235523E-4 3.265551691936213E-6 9.470099906615019E-5 1.8287089474842793E-4 70-71 0.5098914050581502 7.51076889145329E-4 3.265551691936213E-6 9.79665507580864E-5 1.8776922228633226E-4 72-73 0.5781267404370033 7.837324060646911E-4 3.265551691936213E-6 1.0449765414195882E-4 1.9430032567020468E-4 74-75 0.651502054178964 8.082240437542127E-4 3.265551691936213E-6 1.0776320583389503E-4 1.99198653208109E-4 76-77 0.7300124479564944 8.212862505219576E-4 3.265551691936213E-6 1.0776320583389503E-4 2.0409698074601334E-4 78-79 0.8152515109952595 8.376140089816388E-4 3.265551691936213E-6 1.1429430921776747E-4 2.0899530828391764E-4 80-81 0.9061024246166169 8.702695259010008E-4 3.265551691936213E-6 1.2245818844760798E-4 2.1552641166779006E-4 82-83 1.0041097947708526 8.963939394364905E-4 3.265551691936213E-6 1.289892918314804E-4 2.204247392056944E-4 84-85 1.1078922930922772 9.241511288179483E-4 3.265551691936213E-6 1.3388761936938473E-4 2.3511972181940736E-4 86-87 1.2187087897581326 9.50275542353438E-4 3.265551691936213E-6 1.3715317106132094E-4 2.3838527351134357E-4 88 1.30577329619269 9.633377491211829E-4 3.265551691936213E-6 1.3715317106132094E-4 2.3838527351134357E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 180265 0.0 83.20621 8 AGTGGTA 183725 0.0 81.8963 5 GTGGTAT 183640 0.0 81.842545 6 GTATCAA 183510 0.0 81.571815 9 TGGTATC 183805 0.0 81.50457 7 AAGCAGT 191975 0.0 79.78276 1 GCAGTGG 195520 0.0 77.846596 3 CAGTGGT 195620 0.0 77.18844 4 AGCAGTG 202355 0.0 75.69159 2 CAACGCA 177070 0.0 44.698856 12-13 ATCAACG 179350 0.0 44.17342 10-11 ACGCAGA 181365 0.0 43.649353 14-15 AGAGTAC 182650 0.0 42.78614 18-19 TCAACGC 176780 0.0 42.402744 12-13 AACGCAG 179895 0.0 41.590122 14-15 AGTACTT 184400 0.0 41.55637 20-21 GAGTACT 176055 0.0 40.934456 20-21 CGCAGAG 183840 0.0 40.567234 16-17 TATCAAC 186135 0.0 40.190525 10-11 GCAGAGT 196750 0.0 39.91025 16-17 >>END_MODULE