FastQCFastQC Report
Thu 26 May 2016
SRR1660586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30622697
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13264924.331728194939851No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT639620.20887121731962408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA622830.20338835602886318No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT381850.1246950913565843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1727500.082.6770868
AGTGGTA1765950.081.3239755
GTGGTAT1765600.081.148486
GTATCAA1759150.080.997289
TGGTATC1765900.080.905867
AAGCAGT1828500.079.062931
GCAGTGG1879000.077.006273
CAGTGGT1876800.076.771074
AGCAGTG1957050.074.204732
CAACGCA1682500.044.58761212-13
ATCAACG1707150.043.96994810-11
ACGCAGA1730950.043.41695414-15
AGAGTAC1760500.042.4198618-19
TCAACGC1681350.042.21470612-13
AACGCAG1711700.041.37286814-15
AGTACTT1776800.041.17721620-21
GAGTACT1691150.040.63075620-21
CGCAGAG1749650.040.41506216-17
TATCAAC1781700.039.9636510-11
GCAGAGT1900000.039.4995816-17
Produced by FastQC (version 0.11.5)