##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30622697
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.502221440521716	34.0	31.0	34.0	31.0	34.0
2	32.78337871416094	34.0	31.0	34.0	31.0	34.0
3	32.869030640900114	34.0	31.0	34.0	31.0	34.0
4	36.28932575729695	37.0	37.0	37.0	35.0	37.0
5	36.21314288548785	37.0	37.0	37.0	35.0	37.0
6	36.2009077123416	37.0	37.0	37.0	35.0	37.0
7	36.13973870426893	37.0	36.0	37.0	35.0	37.0
8	36.16730410779952	37.0	37.0	37.0	35.0	37.0
9	37.93155308952703	39.0	38.0	39.0	35.0	39.0
10-11	37.85119444247513	39.0	38.0	39.0	35.0	39.0
12-13	37.89596742573001	39.0	38.0	39.0	35.0	39.0
14-15	39.4030206581739	41.0	39.0	41.0	36.0	41.0
16-17	39.38633122353659	41.0	39.0	41.0	36.0	41.0
18-19	39.36644718131783	41.0	39.0	41.0	36.0	41.0
20-21	39.316984637244715	41.0	39.0	41.0	36.0	41.0
22-23	39.18991240386175	41.0	39.0	41.0	35.5	41.0
24-25	39.21493613054396	41.0	39.0	41.0	36.0	41.0
26-27	39.1948043472461	41.0	39.0	41.0	36.0	41.0
28-29	39.132207476696124	41.0	39.0	41.0	36.0	41.0
30-31	39.04562176545064	41.0	39.0	41.0	35.0	41.0
32-33	38.99086941950279	40.0	39.0	41.0	35.0	41.0
34-35	38.86500837924237	40.0	38.0	41.0	35.0	41.0
36-37	38.744694956162746	40.0	38.0	41.0	35.0	41.0
38-39	38.44466806761011	40.0	38.0	41.0	34.5	41.0
40-41	38.33459353694418	40.0	38.0	41.0	34.0	41.0
42-43	38.23873579782996	40.0	37.0	41.0	34.0	41.0
44-45	38.14596594806787	40.0	37.0	41.0	34.0	41.0
46-47	38.01808898478145	40.0	37.0	41.0	33.0	41.0
48-49	37.8713154167969	40.0	37.0	41.0	33.0	41.0
50-51	37.69674192968699	40.0	36.0	41.0	33.0	41.0
52-53	37.36654633979496	40.0	36.0	41.0	33.0	41.0
54-55	36.804985383880464	39.5	36.0	41.0	31.5	41.0
56-57	36.29406768123657	39.0	35.0	41.0	30.5	41.0
58-59	36.21855527617309	39.0	35.0	41.0	30.5	41.0
60-61	36.014950822261014	39.0	35.0	41.0	30.0	41.0
62-63	35.71377021429562	38.5	35.0	40.5	30.0	41.0
64-65	35.598068191054494	38.0	35.0	40.0	30.0	41.0
66-67	35.36023371814703	37.0	35.0	40.0	30.0	41.0
68-69	35.052822241620326	37.0	35.0	40.0	30.0	41.0
70-71	34.712079801462295	36.0	35.0	39.0	29.0	41.0
72-73	34.36385346790324	36.0	34.5	39.0	29.0	41.0
74-75	34.00321024957403	35.0	34.0	38.5	29.0	40.0
76-77	32.446302296626584	34.5	32.0	36.5	26.5	39.0
78-79	33.32278151398618	35.0	34.0	37.0	28.5	39.0
80-81	33.23314522231664	35.0	34.0	36.5	29.0	39.0
82-83	32.985731873975695	35.0	34.0	36.0	29.0	37.5
84-85	32.75196567434932	35.0	34.0	36.0	29.0	37.0
86-87	32.54359731933474	35.0	34.0	35.0	29.0	37.0
88-89	32.36131286868691	35.0	34.0	35.0	28.0	36.0
90-91	32.190611721756575	35.0	34.0	35.0	27.0	36.0
92-93	32.026667262521	35.0	34.0	35.0	27.0	36.0
94-95	31.902117847425394	35.0	34.0	35.0	27.0	36.0
96-97	31.739077537814516	35.0	34.0	35.0	26.0	35.5
98-99	31.600470886022872	35.0	34.0	35.0	25.0	35.0
100	31.509579838771224	35.0	34.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5235743344076198
1101	2	0.3990594021402032
1101	3	0.3372455254573694
1101	4	0.2209736162420768
1101	5	0.2475086525801089
1101	6	0.20555383932610027
1101	7	0.23223814757547245
1101	8	0.20954999420843023
1101	9	0.30489090896156057
1101	10-11	0.29552295435686204
1101	12-13	0.304890940774591
1101	14-15	0.3887183434110639
1101	16-17	0.4062715715806533
1101	18-19	0.34096357427499413
1101	20-21	0.3324759719082948
1101	22-23	0.23651571727425136
1101	24-25	0.34932398671002574
1101	26-27	0.41092059015199567
1101	28-29	0.42153669779693814
1101	30-31	0.3834900332512703
1101	32-33	0.38709120108066486
1101	34-35	0.42426863260778447
1101	36-37	0.3720950189493095
1101	38-39	0.36594844248345737
1101	40-41	0.03299858713300807
1101	42-43	0.12060605248166922
1101	44-45	0.29740451353263353
1101	46-47	0.34764628396987973
1101	48-49	0.37685421604755476
1101	50-51	0.4058752431595849
1101	52-53	0.32161743138739496
1101	54-55	0.26670226833026334
1101	56-57	0.33139456104261455
1101	58-59	0.27981067054391673
1101	60-61	0.30043255713327
1101	62-63	0.2652892409308265
1101	64-65	0.34122213901413545
1101	66-67	0.268239785970664
1101	68-69	0.27919989401231504
1101	70-71	0.310287768087953
1101	72-73	0.27808401397933835
1101	74-75	0.21367116206273096
1101	76-77	0.16931675252084943
1101	78-79	0.30307620789736944
1101	80-81	0.32337586063839296
1101	82-83	0.3093159172169919
1101	84-85	0.33886115154226104
1101	86-87	0.31927666717087533
1101	88-89	0.28092815678642324
1101	90-91	0.2837763047185362
1101	92-93	0.2063754212935578
1101	94-95	0.2620934939730546
1101	96-97	0.23220404762232505
1101	98-99	0.3076514622103268
1101	100	0.3160253395836037
1102	1	0.48234485282105055
1102	2	0.32450636911231356
1102	3	0.2619053633491504
1102	4	0.17296779744008717
1102	5	0.20736800920388987
1102	6	0.21952180348060324
1102	7	0.23080821232281323
1102	8	0.2341284338499392
1102	9	0.28888166164302476
1102	10-11	0.24755517971125585
1102	12-13	0.2809589941593629
1102	14-15	0.3458939293489678
1102	16-17	0.3087438112247014
1102	18-19	0.2793756729919892
1102	20-21	0.25856851256612856
1102	22-23	0.3037302105723896
1102	24-25	0.33238767122033863
1102	26-27	0.3256241433652818
1102	28-29	0.27483815143707346
1102	30-31	0.35142917165885734
1102	32-33	0.3143784912354164
1102	34-35	0.40277694804313313
1102	36-37	0.4021936023594037
1102	38-39	0.3865698505297317
1102	40-41	0.39371603969075863
1102	42-43	0.3755641333997488
1102	44-45	0.33265841437734167
1102	46-47	0.33876035034957397
1102	48-49	0.35992132164374624
1102	50-51	0.2943985319135862
1102	52-53	0.33242705664981287
1102	54-55	0.3056440170412884
1102	56-57	0.22459925937727832
1102	58-59	0.1907819912677482
1102	60-61	0.1465837595832582
1102	62-63	0.12273179495978326
1102	64-65	0.2083361841866207
1102	66-67	0.1960493524115563
1102	68-69	0.17215581852084227
1102	70-71	0.17768585288190053
1102	72-73	0.1208534547303799
1102	74-75	0.19105245953538486
1102	76-77	-0.07660067030342788
1102	78-79	0.2346960856890803
1102	80-81	0.22894051106646884
1102	82-83	0.06783249831041616
1102	84-85	0.1529220843496688
1102	86-87	0.23586000063302492
1102	88-89	0.25206064767295544
1102	90-91	0.27105151951133877
1102	92-93	0.2528957145702506
1102	94-95	0.24258994167097114
1102	96-97	0.31475654081560833
1102	98-99	0.35940319377742114
1102	100	0.37326864349614297
1103	1	0.447152320485408
1103	2	0.3098024992749302
1103	3	0.2588687347358629
1103	4	0.1967086657839019
1103	5	0.17476274210144283
1103	6	0.17455101631151138
1103	7	0.2136920487061147
1103	8	0.21710666826915315
1103	9	0.24051307481056483
1103	10-11	0.24732339065362652
1103	12-13	0.24892226479249047
1103	14-15	0.28283290621428847
1103	16-17	0.3026014128235417
1103	18-19	0.3189867299353679
1103	20-21	0.32512477424765507
1103	22-23	0.34238953519140836
1103	24-25	0.3336788210378572
1103	26-27	0.30391654794967593
1103	28-29	0.29704013777037375
1103	30-31	0.36782500189159606
1103	32-33	0.32838115376744526
1103	34-35	0.4084395900899196
1103	36-37	0.35060127919688
1103	38-39	0.29600550699360895
1103	40-41	0.30203901592830107
1103	42-43	0.3098386509075368
1103	44-45	0.3079835033296163
1103	46-47	0.3136119080300688
1103	48-49	0.3434497647475112
1103	50-51	0.3238995548190502
1103	52-53	0.34808457064433895
1103	54-55	0.2996941001571898
1103	56-57	0.257392901431615
1103	58-59	0.26053343321466826
1103	60-61	0.2492302469374792
1103	62-63	0.23601177487871894
1103	64-65	0.21409945710257716
1103	66-67	0.20747732776166572
1103	68-69	0.23495833731817584
1103	70-71	0.16841047853716162
1103	72-73	0.2055245297631032
1103	74-75	0.2419747641026575
1103	76-77	0.29250172921259576
1103	78-79	0.2775024171977165
1103	80-81	0.2735153473299974
1103	82-83	0.2871817521264646
1103	84-85	0.26573056108971116
1103	86-87	0.2656772135550156
1103	88-89	0.24621326444836455
1103	90-91	0.21231904599947882
1103	92-93	0.19898679002677966
1103	94-95	0.20133415435288526
1103	96-97	0.23491253872414575
1103	98-99	0.24173165255202278
1103	100	0.276515593868357
1104	1	0.3845572633427423
1104	2	0.2435269495171255
1104	3	0.17779370223189517
1104	4	0.15540910768792315
1104	5	0.13781124844785353
1104	6	0.1667390901407444
1104	7	0.15652364728178725
1104	8	0.16173058341674107
1104	9	0.20706219619967925
1104	10-11	0.16065186478866167
1104	12-13	0.1786030129788614
1104	14-15	0.20670438108486167
1104	16-17	0.2512215478884414
1104	18-19	0.263836259840339
1104	20-21	0.2411445838100832
1104	22-23	0.2628247734901592
1104	24-25	0.281571416693545
1104	26-27	0.2539769739350035
1104	28-29	0.2064283434753591
1104	30-31	0.2821610528507037
1104	32-33	0.25399294534862804
1104	34-35	0.34247414534728904
1104	36-37	0.24002909758229407
1104	38-39	0.2673058495450604
1104	40-41	0.30065057689809294
1104	42-43	0.3005179416266728
1104	44-45	0.18935909174091137
1104	46-47	0.20202995519873923
1104	48-49	0.290898268497493
1104	50-51	0.27100805664213823
1104	52-53	0.271082337695681
1104	54-55	0.290411427425326
1104	56-57	0.163300583005217
1104	58-59	0.14287403251246644
1104	60-61	0.15407763530185292
1104	62-63	0.13612130122899657
1104	64-65	0.09756654671258502
1104	66-67	0.11406591145330225
1104	68-69	0.07850854681415598
1104	70-71	0.10550087356303806
1104	72-73	0.11918731722734321
1104	74-75	0.09859138714708138
1104	76-77	-0.1846155498373534
1104	78-79	0.12665535080366652
1104	80-81	0.14336251086429996
1104	82-83	0.1626781775958719
1104	84-85	0.1304321009808831
1104	86-87	0.04013495769729758
1104	88-89	0.1011113200747431
1104	90-91	0.15991789389101996
1104	92-93	0.1350189530007384
1104	94-95	0.15105802595324747
1104	96-97	0.19148787403097245
1104	98-99	0.2121080127288799
1104	100	0.18957782689614433
1105	1	0.40655418862492354
1105	2	0.2669563927186047
1105	3	0.1954311617644322
1105	4	0.14441520009076214
1105	5	0.12205362690850308
1105	6	0.12234933983764762
1105	7	0.14920114287548358
1105	8	0.13394713225253696
1105	9	0.17408176523400698
1105	10-11	0.17533239349724994
1105	12-13	0.20270452147207862
1105	14-15	0.22625193083298
1105	16-17	0.25520966331300343
1105	18-19	0.22086738095020308
1105	20-21	0.22111197236424118
1105	22-23	0.23742004774126002
1105	24-25	0.15939700918759314
1105	26-27	0.21541740472428472
1105	28-29	0.2037564001702421
1105	30-31	0.2409462283862709
1105	32-33	0.20989514826860045
1105	34-35	0.15380749033067076
1105	36-37	0.15644144548960526
1105	38-39	0.12596915926420849
1105	40-41	0.1397544547875924
1105	42-43	0.2112112898903078
1105	44-45	0.267559703274479
1105	46-47	0.2635935637043332
1105	48-49	0.26394538988785854
1105	50-51	0.2147296499579454
1105	52-53	0.2053302609112322
1105	54-55	0.18644672817382002
1105	56-57	0.14662194221147473
1105	58-59	0.12279650827447597
1105	60-61	0.0870816696018224
1105	62-63	0.12179972474211809
1105	64-65	0.0939221366588896
1105	66-67	0.13398128203167659
1105	68-69	0.16813848327274883
1105	70-71	0.14612532064402473
1105	72-73	0.1395942540943551
1105	74-75	0.07147045900372717
1105	76-77	-0.05431474972119332
1105	78-79	0.1545770887324025
1105	80-81	0.11264440401513554
1105	82-83	0.12240127652875543
1105	84-85	0.11743957354254064
1105	86-87	0.10243356195047681
1105	88-89	0.10717194565121702
1105	90-91	0.10044787804007171
1105	92-93	0.09573780579074764
1105	94-95	0.1327588026722566
1105	96-97	0.09418687164015438
1105	98-99	0.0946786396473307
1105	100	0.14135185933712435
1106	1	0.39277216378307855
1106	2	0.24409941443022376
1106	3	0.1782952725470608
1106	4	0.13513846494173976
1106	5	0.14246292397654514
1106	6	0.13938528279405915
1106	7	0.10821188849507024
1106	8	0.12893786408849905
1106	9	0.15303909943771998
1106	10-11	0.1310050785976884
1106	12-13	0.19649885002122147
1106	14-15	0.2136047631714888
1106	16-17	0.1986844771006986
1106	18-19	0.15482788349819288
1106	20-21	0.2059033183835055
1106	22-23	0.25349453737766936
1106	24-25	0.2125204919467123
1106	26-27	0.20169732790508732
1106	28-29	0.17842699402454087
1106	30-31	0.30443389190563863
1106	32-33	0.2222292912716881
1106	34-35	0.17989275192584842
1106	36-37	0.19331028186201138
1106	38-39	0.12286532281981977
1106	40-41	0.2653890766684128
1106	42-43	0.28802309450681207
1106	44-45	0.2200162085036652
1106	46-47	0.24521726341326655
1106	48-49	0.30438656669203823
1106	50-51	0.33364272380157445
1106	52-53	0.3191483194297078
1106	54-55	0.24802677764424885
1106	56-57	0.30548594642756655
1106	58-59	0.29633309984917133
1106	60-61	0.2868277131289787
1106	62-63	0.27801715855232345
1106	64-65	0.24108920196243133
1106	66-67	0.22914591817471575
1106	68-69	0.2008023922150457
1106	70-71	0.20800535314509716
1106	72-73	0.2243594781206113
1106	74-75	0.2279343878377844
1106	76-77	-0.5770894340704658
1106	78-79	0.22600113820916334
1106	80-81	0.22343766631029638
1106	82-83	0.22377325460497843
1106	84-85	0.27497808550652536
1106	86-87	0.2480558626746685
1106	88-89	0.21102313297527076
1106	90-91	0.2425837361394727
1106	92-93	0.2210166620475036
1106	94-95	0.2163037602923623
1106	96-97	0.27215773705090385
1106	98-99	0.3101698452453441
1106	100	0.34450106100977607
1107	1	0.5109631175690978
1107	2	0.3678808527296482
1107	3	0.3001232722759397
1107	4	0.19095637762166717
1107	5	0.23265280595531834
1107	6	0.20425793239983392
1107	7	0.17989578715936716
1107	8	0.2270584390493866
1107	9	0.24987222362483408
1107	10-11	0.2746273818211691
1107	12-13	0.3116184849108521
1107	14-15	0.31903524446062903
1107	16-17	0.33144428917890423
1107	18-19	0.3403083384958805
1107	20-21	0.3480990822308385
1107	22-23	0.36995459870383485
1107	24-25	0.3915830533048137
1107	26-27	0.4107050154976122
1107	28-29	0.3987435166290183
1107	30-31	0.41334258292237536
1107	32-33	0.4135419108293803
1107	34-35	0.4112593961247768
1107	36-37	0.36867578222847897
1107	38-39	0.39576968098067056
1107	40-41	0.45564280943262503
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2316	3	-1.2844906781541816
2316	4	-0.718205038576933
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2316	54-55	-0.6889419654285831
2316	56-57	-0.5873163287532677
2316	58-59	-0.5383644462271988
2316	60-61	-0.5201499556486695
2316	62-63	-0.6155101413023658
2316	64-65	-0.5256473667832324
2316	66-67	-0.5026987551973932
2316	68-69	-0.5082098903820906
2316	70-71	-0.5050497926333151
2316	72-73	-0.4784770302072303
2316	74-75	-0.5102973335351848
2316	76-77	-0.4522242715742735
2316	78-79	-0.533620766237334
2316	80-81	-0.46061011152573883
2316	82-83	-0.46361596597010646
2316	84-85	-0.4512541058811266
2316	86-87	-0.4551501169816561
2316	88-89	-0.43680758638973316
2316	90-91	-0.47800122212569107
2316	92-93	-0.47665778111172585
2316	94-95	-0.5051931701642758
2316	96-97	-0.4574574047999249
2316	98-99	-0.4456161336841582
2316	100	-0.4603243784358284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	172.0
3	82.0
4	141.0
5	387.0
6	763.0
7	1722.0
8	3782.0
9	6347.0
10	9397.0
11	13679.0
12	18579.0
13	25042.0
14	27363.0
15	27646.0
16	35159.0
17	50518.0
18	78748.0
19	141276.0
20	328728.0
21	418212.0
22	160334.0
23	110270.0
24	121678.0
25	136868.0
26	160068.0
27	197439.0
28	249499.0
29	315495.0
30	390040.0
31	486260.0
32	615371.0
33	798531.0
34	1107309.0
35	1825031.0
36	3276562.0
37	7022105.0
38	9457490.0
39	2994475.0
40	10129.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.165894368837737	33.47922929381484	27.250880502493413	22.10399583485401
2	21.912573929465673	31.90779947718457	27.261404022094748	18.918222571255004
3	25.258343509064535	26.026371877042703	26.61056927807502	22.104715335817744
4	20.88235402649218	27.273094201990112	25.80714232975626	26.03740944176145
5	22.15916841028078	31.01342771996862	25.867809749089048	20.95959412066155
6	26.72150006905009	26.20891621662194	26.188130980102763	20.881452734225206
7	21.67726768155006	26.281127361185725	30.858669959736073	21.18293499752814
8	26.112487740710755	26.06176392627991	26.29342869440925	21.53231963860009
9	25.914654088109874	25.590662376994423	26.900040842255013	21.594642692640694
10-11	21.202877721710795	28.8221315059219	28.666797375815722	21.308193396551584
12-13	21.265511003162132	26.397100163973146	28.41562256910291	23.92176626376181
14-15	21.26500974097742	31.338769736708688	25.959661554303988	21.436558968009905
16-17	23.927203407328886	26.187048449716887	25.984693640798522	23.901054502155706
18-19	21.47354134092108	28.724483346453777	25.94404895166484	23.857926360960303
20-21	23.978820350147473	28.69491540865914	25.9229240977697	21.403340143423684
22-23	23.84973962286862	26.25462904198151	28.487409191946746	21.40822214320313
24-25	21.501018858982444	28.441787483220406	25.9446897845785	24.112503873218646
26-27	21.580861106983093	25.973480492368278	30.950469009157246	21.495189391491387
28-29	21.646550488447403	26.023504836650428	30.882463142190115	21.447481532712054
30-31	21.663296659733273	25.979244376615846	30.83699990120352	21.520459062447365
32-33	21.64668250712037	26.02845032337991	30.78416844715102	21.5406987223487
34-35	21.68127250555277	26.086306340890292	30.738274900178208	21.49414625337873
36-37	21.65647833813839	26.056976767044965	30.727643112990126	21.55890178182652
38-39	21.657547014882457	26.021932359517518	30.757272946925607	21.563247678674415
40-41	21.652829925463458	26.080152247857203	30.761421830350216	21.505595996329127
42-43	21.66281108420986	26.070936860982556	30.703415835646354	21.562836219161234
44-45	21.671079461093843	26.052817947419847	30.722912812023058	21.553189779463253
46-47	21.665132891462825	26.116052090382503	30.696319791819775	21.522495226334897
48-49	21.711356775662182	26.096693899952704	30.603989583281972	21.587959741103145
50-51	21.724113653346734	26.11370905704354	30.584371781492663	21.577805508117066
52-53	21.77274937059956	26.253224236767004	30.379744375809466	21.594282016823975
54-55	22.008973583943668	26.458468721847712	29.735678718098264	21.796878976110364
56-57	22.320557931373557	26.820726984953495	28.89350944352671	21.965205640146234
58-59	22.36312662547761	27.184314205193438	28.48559477492161	21.96696439440735
60-61	22.31930918019525	27.33209736859685	28.333455559695476	22.015137891512428
62-63	22.277846133677723	27.43861685284246	28.284807159729574	21.99872985375024
64-65	22.22355403716678	27.49990223651025	28.337463075403445	21.93908065091952
66-67	22.173112308788653	27.30216162409625	28.54557612789927	21.979149939215826
68-69	22.102496393443072	27.128477612536873	28.827637226074504	21.941388767945554
70-71	22.052624888003823	27.065839432757997	29.038399197823754	21.843136481414422
72-73	22.021015967991683	27.010713550672882	29.08340365841741	21.884866822918024
74-75	22.057392545030666	27.059474661401378	29.015049234084216	21.86808355948374
76-77	22.09003994651418	27.18821108408577	28.843009810664295	21.87873915873576
78-79	22.161377455277652	27.31055426302606	28.55557937605141	21.972488905644873
80-81	22.199892267731588	27.43544861948945	28.34708537835346	22.017573734425504
82-83	22.195752846112672	27.627570030417786	28.142298503507806	22.034378619961736
84-85	22.178839630677803	27.716963787691505	28.00914812022623	22.095048461404463
86-87	22.207057313877858	27.74567535446496	27.921202619981205	22.126064711675973
88-89	22.21056358196646	27.82099185864731	27.837575823320897	22.130868736065327
90-91	22.247138934229	27.810580314347806	27.731838764000706	22.210441987422488
92-93	22.255162644600926	27.84003050622085	27.688446435042625	22.2163604141356
94-95	22.268765223389696	27.89673783468517	27.659379577180935	22.175117364744196
96-97	22.288859795726026	27.863935367939668	27.59529312522669	22.251911711107613
98-99	22.26163523228437	27.839585850607655	27.609806008136545	22.28897290897143
100	22.263729957986683	27.89764067616307	27.556115939196946	22.282513426653296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124730.0
1	73482.0
2	16381.0
3	9509.5
4	8174.0
5	7675.5
6	7708.0
7	7709.5
8	7355.5
9	7281.5
10	8026.0
11	14850.0
12	164197.5
13	187001.0
14	62009.5
15	60050.0
16	66511.5
17	74229.0
18	81544.0
19	88886.0
20	98280.5
21	111580.0
22	131280.0
23	155672.5
24	181782.0
25	211623.5
26	246388.0
27	288345.0
28	336103.0
29	381042.0
30	423307.5
31	465214.5
32	508559.0
33	550191.5
34	589802.5
35	636401.5
36	687569.0
37	732407.5
38	777361.5
39	817044.5
40	842605.5
41	875112.0
42	915186.5
43	943220.0
44	969037.0
45	1001220.5
46	1028898.0
47	1043332.5
48	1049002.0
49	1053750.5
50	1048944.0
51	1042465.5
52	1037935.5
53	1018812.0
54	982627.0
55	936502.5
56	883664.5
57	818169.5
58	738709.5
59	648833.0
60	545949.5
61	445314.5
62	356397.5
63	273548.0
64	203746.5
65	148534.5
66	105617.5
67	76372.5
68	55492.5
69	41208.0
70	32075.0
71	25652.0
72	21560.0
73	17424.0
74	13494.0
75	11199.0
76	9045.0
77	6252.0
78	4079.0
79	2653.5
80	1616.0
81	1024.5
82	628.0
83	350.5
84	222.5
85	131.0
86	75.0
87	47.0
88	22.0
89	15.5
90	9.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02822742882509663
2	0.01135758878455415
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007082981619809647
26-27	0.028549085666752343
28-29	0.03327270618913807
30-31	0.03023411033979143
32-33	0.03726157758083816
34-35	0.029850408015988924
36-37	2.44916376895216E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.005520415135218168
54-55	0.03324005067221872
56-57	0.04590712568523928
58-59	0.05074340774099682
60-61	0.05523843964494701
62-63	0.03677501037873967
64-65	0.0010596715240333012
66-67	1.4694982613712958E-5
68-69	0.0
70-71	0.0
72-73	9.633377491211829E-5
74-75	2.9389965227425916E-5
76-77	0.0
78-79	4.6370834025494224E-4
80-81	0.016750647403786807
82-83	0.04075571789120991
84-85	0.055013116578203416
86-87	0.05894320803944865
88-89	0.058231317770606555
90-91	0.03643865855447024
92-93	0.002436101562184415
94-95	0.0
96-97	0.0
98-99	4.049284098000905E-4
100	8.163879229840533E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.0622697E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.28906051421431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65479352026095	35.30355048455795
2	12.499707684567282	11.821988662109838
3	4.563179698812118	6.473654427430817
4	2.3762007669326435	4.494732074456009
5	1.4260569662676088	3.371844708727292
6	0.8765462393225689	2.487062889289917
7	0.6321537536341598	2.0925769976925457
8	0.44650727295037695	1.6891927560469748
9	0.34549182271151996	1.4704185340234144
>10	1.9739500313458156	17.624283979343144
>50	0.17120677900006287	5.491567517347661
>100	0.03338732425774013	2.1995613404474623
>500	2.848963538499884E-4	0.09088902556423989
>1k	4.7123916266401814E-4	0.4222399784051271
>5k	3.444690038007929E-5	0.10912739254825204
>10k+	2.755752023728094E-5	4.85730923200927
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1326492	4.331728194939851	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63962	0.20887121731962408	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62283	0.20338835602886318	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38185	0.1246950913565843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017503357068778102	1.6327758459681065E-5	0.0	0.0	0.0
2	0.0017829912237971725	2.285886184355349E-5	0.0	3.265551691936213E-6	0.0
3	0.0018287089474842795	2.938996522742592E-5	0.0	6.531103383872426E-6	3.265551691936213E-6
4	0.0018646300160955777	4.571772368710698E-5	0.0	6.531103383872426E-6	9.79665507580864E-6
5	0.0018809577745552588	6.531103383872426E-5	0.0	9.79665507580864E-6	1.3062206767744853E-5
6	0.0019103477397826847	6.857658553066047E-5	0.0	9.79665507580864E-6	1.6327758459681065E-5
7	0.0019332066016262382	7.510768891453291E-5	0.0	9.79665507580864E-6	1.6327758459681065E-5
8	0.0019658621185456005	8.490434399034154E-5	0.0	1.3062206767744853E-5	1.6327758459681065E-5
9	0.00199198653208109	9.79665507580864E-5	0.0	1.3062206767744853E-5	1.6327758459681065E-5
10-11	0.002037704255768197	1.1755986090970368E-4	0.0	1.3062206767744853E-5	1.959331015161728E-5
12-13	0.00208668753114724	1.436842744451934E-4	0.0	1.3062206767744853E-5	2.4491637689521596E-5
14-15	0.0021422019099101557	1.5674648121293824E-4	0.0	1.959331015161728E-5	2.938996522742592E-5
16-17	0.0022075129437488803	1.6654313628874687E-4	0.0	1.959331015161728E-5	3.1022741073394025E-5
18-19	0.0022858861843553496	1.959331015161728E-4	0.0	2.1226085997585385E-5	3.7553844457266454E-5
20-21	0.002343033338964233	2.1879196335972627E-4	0.0	2.7757189381457814E-5	4.245217199517077E-5
22-23	0.00241487547618683	2.3511972181940736E-4	0.0	2.938996522742592E-5	4.735049953307509E-5
24-25	0.0025112092510989475	2.547130319710246E-4	0.0	3.1022741073394025E-5	4.89832753790432E-5
26-27	0.002636932991238492	2.7593911796861003E-4	0.0	3.428829276533024E-5	4.89832753790432E-5
28-29	0.002852459402906282	2.938996522742592E-4	0.0	3.592106861129835E-5	5.224882707097941E-5
30-31	0.003374947673616076	3.1839128996378075E-4	0.0	3.7553844457266454E-5	5.7147154608883734E-5
32-33	0.0044248225425735695	3.445157034992705E-4	0.0	4.245217199517077E-5	6.857658553066047E-5
34-35	0.006359661920045775	3.706401170347602E-4	0.0	4.245217199517077E-5	7.020936137662858E-5
36-37	0.009316618977094016	3.8533509964847317E-4	0.0	4.245217199517077E-5	7.18421372225967E-5
38-39	0.013774097036586948	4.000300822621861E-4	0.0	4.245217199517077E-5	7.18421372225967E-5
40-41	0.02014682116340047	4.081939614920267E-4	0.0	4.245217199517077E-5	7.510768891453291E-5
42-43	0.028447853564302322	4.261544957976758E-4	0.0	4.4084947841138876E-5	7.837324060646912E-5
44-45	0.03915233201046923	4.457478059492931E-4	0.0	4.571772368710698E-5	8.163879229840533E-5
46-47	0.05292316349536424	4.5881001271703796E-4	0.0	4.571772368710698E-5	8.653711983630965E-5
48-49	0.06981259684605834	4.8003609871462337E-4	0.0	4.571772368710698E-5	8.980267152824586E-5
50-51	0.08967041668472245	4.881999779444639E-4	0.0	4.571772368710698E-5	9.143544737421396E-5
52-53	0.11313830391882204	5.028949605581769E-4	0.0	4.89832753790432E-5	1.0286487829599071E-4
54-55	0.14026524182373618	5.224882707097941E-4	0.0	5.224882707097941E-5	1.0939598167986314E-4
56-57	0.17130757620728182	5.37183253323507E-4	0.0	6.0412706300819945E-5	1.1429430921776747E-4
58-59	0.20674860871986553	5.616748910130286E-4	0.0	6.531103383872426E-5	1.1429430921776747E-4
60-61	0.24678590523884947	5.861665287025502E-4	0.0	6.694380968469237E-5	1.1755986090970368E-4
62-63	0.29069288051277786	6.008615113162633E-4	0.0	7.020936137662858E-5	1.2245818844760798E-4
64-65	0.3388662990722209	6.204548214678806E-4	0.0	7.18421372225967E-5	1.2572374013954422E-4
66-67	0.3913649408476334	6.433136833114341E-4	0.0	7.34749130685648E-5	1.3388761936938473E-4
68-69	0.4496501402211569	6.498447866953065E-4	0.0	7.674046476050101E-5	1.3388761936938473E-4
70-71	0.5131406289916267	6.678053210009556E-4	0.0	7.837324060646912E-5	1.3388761936938473E-4
72-73	0.5818511021416566	7.004608379203177E-4	0.0	7.837324060646912E-5	1.4041872275325716E-4
74-75	0.6558746278944667	7.249524756098393E-4	0.0	8.000601645243722E-5	1.436842744451934E-4
76-77	0.7351850818365214	7.494441132993609E-4	0.0	8.163879229840533E-5	1.4531705029116149E-4
78-79	0.8210201080590649	7.65771871759042E-4	0.0	8.163879229840533E-5	1.5021537782906582E-4
80-81	0.9126514885347949	7.788340785267868E-4	0.0	8.327156814437344E-5	1.5348092952100203E-4
82-83	1.0115405576458534	8.11489595446149E-4	0.0	8.490434399034154E-5	1.5348092952100203E-4
84-85	1.116309252578243	8.327156814437344E-4	0.0	8.980267152824586E-5	1.6327758459681066E-4
86-87	1.228407478283183	8.555745432872879E-4	0.0	9.470099906615019E-5	1.6980868798068308E-4
88	1.316376542536407	8.816989568227776E-4	0.0	9.79665507580864E-5	1.6980868798068308E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	172750	0.0	82.677086	8
AGTGGTA	176595	0.0	81.323975	5
GTGGTAT	176560	0.0	81.14848	6
GTATCAA	175915	0.0	80.99728	9
TGGTATC	176590	0.0	80.90586	7
AAGCAGT	182850	0.0	79.06293	1
GCAGTGG	187900	0.0	77.00627	3
CAGTGGT	187680	0.0	76.77107	4
AGCAGTG	195705	0.0	74.20473	2
CAACGCA	168250	0.0	44.587612	12-13
ATCAACG	170715	0.0	43.969948	10-11
ACGCAGA	173095	0.0	43.416954	14-15
AGAGTAC	176050	0.0	42.41986	18-19
TCAACGC	168135	0.0	42.214706	12-13
AACGCAG	171170	0.0	41.372868	14-15
AGTACTT	177680	0.0	41.177216	20-21
GAGTACT	169115	0.0	40.630756	20-21
CGCAGAG	174965	0.0	40.415062	16-17
TATCAAC	178170	0.0	39.96365	10-11
GCAGAGT	190000	0.0	39.49958	16-17
>>END_MODULE