FastQCFastQC Report
Thu 26 May 2016
SRR1660585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30083105
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11674373.880706462979802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA351090.11670670298162374No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT324320.10780802048192832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1715400.076.850281
GTGGTAT1729650.074.913366
AGTGGTA1739250.074.6173555
GGTATCA1734850.074.586468
TGGTATC1746950.073.967017
GTATCAA1753400.073.541119
GCAGTGG1816400.072.203523
CAGTGGT1818250.071.6368944
AGCAGTG1844150.071.557982
AGTACTT1576750.041.3545820-21
CAACGCA1696000.040.40114212-13
GAGTACT1523950.040.12946320-21
ATCAACG1712700.040.1174110-11
ACGCAGA1707950.040.03882614-15
AGAGTAC1698950.039.68578718-19
GTACTTT1568500.038.88571522-23
TACTTTT1696950.038.4788222-23
TCAACGC1693600.038.05311612-13
AACGCAG1699950.037.6803114-15
GCAGAGT1815500.037.41749616-17
Produced by FastQC (version 0.11.5)