##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660585_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30083105
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05633999548916	34.0	31.0	34.0	30.0	34.0
2	32.20546602486678	34.0	31.0	34.0	30.0	34.0
3	32.21621006209299	34.0	31.0	34.0	30.0	34.0
4	35.60896004584633	37.0	35.0	37.0	35.0	37.0
5	35.58923864408278	37.0	35.0	37.0	35.0	37.0
6	35.61348481149137	37.0	35.0	37.0	35.0	37.0
7	35.59242604777665	37.0	35.0	37.0	35.0	37.0
8	35.601128008561616	37.0	35.0	37.0	35.0	37.0
9	37.285138452297396	39.0	38.0	39.0	35.0	39.0
10-11	37.2313851578818	39.0	37.0	39.0	34.5	39.0
12-13	37.24202624695822	39.0	38.0	39.0	35.0	39.0
14-15	38.65183126874703	41.0	39.0	41.0	35.0	41.0
16-17	38.62638148555477	41.0	38.5	41.0	35.0	41.0
18-19	38.57665004992005	41.0	38.5	41.0	34.0	41.0
20-21	38.52315743338329	40.0	39.0	41.0	34.0	41.0
22-23	38.388521813157254	40.0	38.5	41.0	34.0	41.0
24-25	38.363083730884824	40.0	38.0	41.0	34.0	41.0
26-27	38.32300204716235	40.0	38.0	41.0	34.0	41.0
28-29	38.19934699227357	40.0	38.0	41.0	34.0	41.0
30-31	38.08785670561599	40.0	38.0	41.0	33.5	41.0
32-33	37.98462153424654	40.0	38.0	41.0	33.0	41.0
34-35	37.85671341106578	40.0	38.0	41.0	33.0	41.0
36-37	37.66392544918485	40.0	38.0	41.0	33.0	41.0
38-39	37.300746465499486	40.0	37.0	41.0	32.5	41.0
40-41	37.15863643397183	40.0	36.0	41.0	32.0	41.0
42-43	36.983148980133535	40.0	36.0	41.0	31.5	41.0
44-45	36.82303532165314	40.0	35.5	41.0	31.0	41.0
46-47	36.62876652858806	40.0	35.0	41.0	31.0	41.0
48-49	36.43039230824079	39.0	35.0	41.0	30.5	41.0
50-51	35.18886979917798	38.0	34.0	40.0	28.5	40.5
52-53	35.16718651548768	38.5	34.5	39.5	28.5	40.5
54-55	35.50125633640543	39.0	35.0	40.5	28.0	41.0
56-57	35.433508692005034	39.0	35.0	41.0	27.5	41.0
58-59	35.25589190344547	39.0	35.0	41.0	27.0	41.0
60-61	35.00035305531128	39.0	35.0	41.0	26.5	41.0
62-63	34.692067474417954	38.0	35.0	41.0	26.0	41.0
64-65	34.36202734059533	37.0	35.0	40.0	26.0	41.0
66-67	34.012116053180016	37.0	34.5	40.0	25.5	41.0
68-69	33.657223215489225	36.0	34.0	39.0	25.0	41.0
70-71	33.301520803786715	35.5	34.0	39.0	24.0	41.0
72-73	32.93047105676093	35.0	34.0	38.5	23.5	40.5
74-75	32.56562189973408	35.0	34.0	37.0	22.5	40.0
76-77	32.21399843865851	35.0	33.0	37.0	20.5	39.0
78-79	31.87721458606085	35.0	33.0	36.0	20.0	39.0
80-81	31.567685084368783	35.0	33.0	36.0	18.5	38.0
82-83	31.19732865008449	35.0	33.0	35.5	14.0	37.0
84-85	30.953822220146492	35.0	33.0	35.0	10.5	37.0
86-87	30.676546653013375	35.0	32.5	35.0	6.0	36.0
88-89	30.4981445897955	35.0	32.0	35.0	2.0	36.0
90-91	30.311797053528885	35.0	32.0	35.0	2.0	36.0
92-93	30.13383453935357	35.0	32.0	35.0	2.0	35.5
94-95	29.904020462648386	35.0	32.0	35.0	2.0	35.0
96-97	29.736516559710175	35.0	32.0	35.0	2.0	35.0
98-99	29.54808986638846	35.0	31.5	35.0	2.0	35.0
100	29.397007157339644	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3671210139004728
1101	2	0.3199197746138438
1101	3	0.378056221417161
1101	4	0.27380097282321714
1101	5	0.24640838510563157
1101	6	0.2648774122602262
1101	7	0.24148760931309
1101	8	0.3019649864525036
1101	9	0.3660573913315446
1101	10-11	0.390568385290166
1101	12-13	0.3641100890879301
1101	14-15	0.45127264737404005
1101	16-17	0.45541101279523133
1101	18-19	0.4096619710270062
1101	20-21	0.4099224759977673
1101	22-23	0.4037850101781473
1101	24-25	0.4416484565206318
1101	26-27	0.4041285362022293
1101	28-29	0.39792281834616716
1101	30-31	0.41299627927154603
1101	32-33	0.43085009047930356
1101	34-35	0.4017368960152581
1101	36-37	0.41185978158162584
1101	38-39	0.4335185918776219
1101	40-41	0.42605996015223013
1101	42-43	0.4825009816848791
1101	44-45	0.4565278670237518
1101	46-47	0.4138272027641392
1101	48-49	0.4279309369493163
1101	50-51	0.3662139558438824
1101	52-53	0.3709706543873139
1101	54-55	0.4378813416605425
1101	56-57	0.44402424744057356
1101	58-59	0.4079454077000051
1101	60-61	0.38717192693207636
1101	62-63	0.38847278114005235
1101	64-65	0.36499410881991423
1101	66-67	0.3764331348269536
1101	68-69	0.3101874070882573
1101	70-71	0.29164855168917825
1101	72-73	0.2693837508810262
1101	74-75	0.2771689327743587
1101	76-77	0.29561545725812977
1101	78-79	0.2461824741801344
1101	80-81	0.2730784063564258
1101	82-83	0.20329798143803401
1101	84-85	0.17078031951369255
1101	86-87	0.11367948632110725
1101	88-89	0.08042473959950058
1101	90-91	0.017014505635806643
1101	92-93	0.07038987397611152
1101	94-95	0.046194361764019476
1101	96-97	-0.03463335993572869
1101	98-99	0.015298817163806433
1101	100	0.006300454456006577
1102	1	0.3120370298987396
1102	2	0.3443951881955982
1102	3	0.3524180062800468
1102	4	0.25999057646473034
1102	5	0.2282230923785491
1102	6	0.22580204041201313
1102	7	0.2818686277453608
1102	8	0.2912556680718055
1102	9	0.3794648648523733
1102	10-11	0.2855135590290274
1102	12-13	0.3463599375997575
1102	14-15	0.4048813944662015
1102	16-17	0.4115402045726242
1102	18-19	0.3564018724160789
1102	20-21	0.38361769737927887
1102	22-23	0.40706211607659526
1102	24-25	0.44001306364761916
1102	26-27	0.43464934024999025
1102	28-29	0.4438378661387645
1102	30-31	0.443970072200095
1102	32-33	0.3891966798550328
1102	34-35	0.3612268320735055
1102	36-37	0.42892918518570156
1102	38-39	0.46799163618948825
1102	40-41	0.365035771458615
1102	42-43	0.4197379965221373
1102	44-45	0.42844193877610337
1102	46-47	0.3621848555176186
1102	48-49	0.39983941572046433
1102	50-51	0.36878040703997783
1102	52-53	0.45769746611624385
1102	54-55	0.5044064635354317
1102	56-57	0.5656588514697205
1102	58-59	0.5185205505151416
1102	60-61	0.4847358946558842
1102	62-63	0.4679836849219754
1102	64-65	0.5015670012196196
1102	66-67	0.47474080855445067
1102	68-69	0.47946387074542685
1102	70-71	0.43643504008473855
1102	72-73	0.4541589359019085
1102	74-75	0.39489556118616065
1102	76-77	0.40892703058584345
1102	78-79	0.45289094835458954
1102	80-81	0.4297659364783186
1102	82-83	0.32445331649661213
1102	84-85	0.23666622289209016
1102	86-87	0.24557942734143623
1102	88-89	0.2546124355635868
1102	90-91	0.3046413531637704
1102	92-93	0.2927039743333637
1102	94-95	0.3218093462145255
1102	96-97	0.289050357231897
1102	98-99	0.30842623200550534
1102	100	0.2892262130334977
1103	1	0.31385265796873085
1103	2	0.30884594087009276
1103	3	0.312555039297969
1103	4	0.2182249668227101
1103	5	0.21270675294846342
1103	6	0.23128817437464022
1103	7	0.1825640776209596
1103	8	0.18037607436103542
1103	9	0.2889986733718857
1103	10-11	0.29178183258450474
1103	12-13	0.30738774165945415
1103	14-15	0.3877127738184356
1103	16-17	0.359356777851211
1103	18-19	0.3828679182168031
1103	20-21	0.38763991644966467
1103	22-23	0.3525183395219855
1103	24-25	0.35926553753191115
1103	26-27	0.28704445573754356
1103	28-29	0.4116654226796541
1103	30-31	0.4356753239127542
1103	32-33	0.37782713108014576
1103	34-35	0.35722051466722604
1103	36-37	0.35496545431674775
1103	38-39	0.3764060444992481
1103	40-41	0.4067604129423401
1103	42-43	0.43358729867418333
1103	44-45	0.37949899762311645
1103	46-47	0.38541771256191737
1103	48-49	0.3796165427839142
1103	50-51	0.42785185330231457
1103	52-53	0.45666210534638907
1103	54-55	0.45002741026750215
1103	56-57	0.4450675643334634
1103	58-59	0.399859370606535
1103	60-61	0.40520980870260104
1103	62-63	0.4117290175484456
1103	64-65	0.42823646008171323
1103	66-67	0.42686481349285543
1103	68-69	0.40277418855728087
1103	70-71	0.38042230028700175
1103	72-73	0.34210877452994026
1103	74-75	0.33761806628987756
1103	76-77	0.36405653125905957
1103	78-79	0.3269135666764349
1103	80-81	0.28415826250620313
1103	82-83	0.2502851851242447
1103	84-85	0.2519946833057958
1103	86-87	0.25065874800525023
1103	88-89	0.20047308705818523
1103	90-91	0.22057258068931063
1103	92-93	0.24017188225315778
1103	94-95	0.21680782828955003
1103	96-97	0.1924491797735115
1103	98-99	0.1911992582349562
1103	100	0.14752524451101223
1104	1	0.21548140976425145
1104	2	0.23714164242121427
1104	3	0.20369355764175623
1104	4	0.1988088229182594
1104	5	0.14270675415139067
1104	6	0.22415167871334063
1104	7	0.217264638293436
1104	8	0.23371834223782884
1104	9	0.31608687630607335
1104	10-11	0.3110443285652167
1104	12-13	0.2816107577603546
1104	14-15	0.34477453560438676
1104	16-17	0.3176207874755974
1104	18-19	0.3592365519786682
1104	20-21	0.27542045668359805
1104	22-23	0.24359831739935345
1104	24-25	0.3096808602779575
1104	26-27	0.31484616308461
1104	28-29	0.3713204675831392
1104	30-31	0.3856065244797051
1104	32-33	0.35341698958338696
1104	34-35	0.3766730130453837
1104	36-37	0.3693412718814031
1104	38-39	0.3523175217066452
1104	40-41	0.34404078594931065
1104	42-43	0.2967275212454439
1104	44-45	0.34187361699179064
1104	46-47	0.34622046457131717
1104	48-49	0.3232616733193936
1104	50-51	0.3108284845511875
1104	52-53	0.43272115168391423
1104	54-55	0.4303428651711698
1104	56-57	0.40981872735383007
1104	58-59	0.42444547032224733
1104	60-61	0.4323920825715888
1104	62-63	0.35525594642363956
1104	64-65	0.3824363069971639
1104	66-67	0.37012442581579563
1104	68-69	0.36144500620594044
1104	70-71	0.28732461603870973
1104	72-73	0.32454512292485305
1104	74-75	0.30528678358703587
1104	76-77	0.33579079262688794
1104	78-79	0.3157979031530651
1104	80-81	0.3436197022444034
1104	82-83	0.25230878583989025
1104	84-85	0.20968485441380835
1104	86-87	0.1685066894695133
1104	88-89	0.19369876880631764
1104	90-91	0.16138428167730368
1104	92-93	0.15112670033663989
1104	94-95	0.16151645298695527
1104	96-97	0.13686155951735302
1104	98-99	0.10348265359725062
1104	100	0.08253255570382478
1105	1	0.22626714227519784
1105	2	0.2277417457406372
1105	3	0.16642361961477548
1105	4	0.18511572083076544
1105	5	0.1676983649145427
1105	6	0.1581672607819371
1105	7	0.20485592471721503
1105	8	0.14243715344201746
1105	9	0.27795576069812
1105	10-11	0.2909758313738706
1105	12-13	0.25175443510013906
1105	14-15	0.32476248211474257
1105	16-17	0.35827891930237854
1105	18-19	0.35663919184278114
1105	20-21	0.2640955634982163
1105	22-23	0.3064698140478299
1105	24-25	0.29834451940940454
1105	26-27	0.3335844772325771
1105	28-29	0.32508597538647876
1105	30-31	0.31780342506589676
1105	32-33	0.31126006486660884
1105	34-35	0.2905362546300907
1105	36-37	0.32727746106682076
1105	38-39	0.2916150589642399
1105	40-41	0.30356261350035396
1105	42-43	0.33071488963195605
1105	44-45	0.32150716658502176
1105	46-47	0.2998139303761107
1105	48-49	0.33153688610416765
1105	50-51	0.45006498193637867
1105	52-53	0.41092671203525555
1105	54-55	0.3580511765525074
1105	56-57	0.30925969097352635
1105	58-59	0.3219115274888793
1105	60-61	0.30869051678472914
1105	62-63	0.2939229777393422
1105	64-65	0.2704581718263199
1105	66-67	0.3093453070363026
1105	68-69	0.27580970418632944
1105	70-71	0.3062394827421002
1105	72-73	0.2386330146850284
1105	74-75	0.22920260570147377
1105	76-77	0.20486027961160147
1105	78-79	0.23347638562588813
1105	80-81	0.23258285437368187
1105	82-83	0.21121141202035076
1105	84-85	0.21423708724059054
1105	86-87	0.1922594953851231
1105	88-89	0.22181054509929155
1105	90-91	0.22195516128679316
1105	92-93	0.20503744984449312
1105	94-95	0.18560732523548396
1105	96-97	0.1064829602172388
1105	98-99	0.13251854946501496
1105	100	0.1560070715317643
1106	1	0.2501574211068984
1106	2	0.2574900541402769
1106	3	0.28344723107820613
1106	4	0.1934912528774717
1106	5	0.16982262373358736
1106	6	0.23949544229103736
1106	7	0.25893834721253484
1106	8	0.25612937765676236
1106	9	0.2965099372785076
1106	10-11	0.31691212370125044
1106	12-13	0.33858336780198073
1106	14-15	0.36558805585042364
1106	16-17	0.32474699589770495
1106	18-19	0.3432975800750242
1106	20-21	0.40325464978976555
1106	22-23	0.4284035712233063
1106	24-25	0.3769026438270302
1106	26-27	0.40259644413214346
1106	28-29	0.43331023547106184
1106	30-31	0.3527944723812553
1106	32-33	0.3257999656341539
1106	34-35	0.4044655408516249
1106	36-37	0.4086963978566942
1106	38-39	0.39873025936636
1106	40-41	0.4301920930817502
1106	42-43	0.4634771758113274
1106	44-45	0.43662245991579596
1106	46-47	0.4407364050585727
1106	48-49	0.38498828072127367
1106	50-51	0.4269449602794495
1106	52-53	0.4531781934609782
1106	54-55	0.48500358776350794
1106	56-57	0.441588920500088
1106	58-59	0.4522249317237623
1106	60-61	0.457539584334441
1106	62-63	0.44250264438332465
1106	64-65	0.42415948860058705
1106	66-67	0.36352009231586635
1106	68-69	0.4228365709117199
1106	70-71	0.4146153606902061
1106	72-73	0.30342839943687494
1106	74-75	0.3893718248819482
1106	76-77	0.4108868060747355
1106	78-79	0.4066108124635157
1106	80-81	0.38489653516046474
1106	82-83	0.32790184539897993
1106	84-85	0.31672735179310507
1106	86-87	0.3291857893977337
1106	88-89	0.29105606165348163
1106	90-91	0.32212559233605376
1106	92-93	0.35882990752870114
1106	94-95	0.41735953348267785
1106	96-97	0.29969472344599524
1106	98-99	0.27084414765963416
1106	100	0.24889397915402967
1107	1	0.3396851622662993
1107	2	0.357325514925229
1107	3	0.39149040095954746
1107	4	0.3345980066771759
1107	5	0.31037884002326876
1107	6	0.36273773667809905
1107	7	0.3270608803597099
1107	8	0.354884734759203
1107	9	0.4712715382375947
1107	10-11	0.4382780559413817
1107	12-13	0.4180354645568869
1107	14-15	0.5511281302949129
1107	16-17	0.4917863127785793
1107	18-19	0.472479598840053
1107	20-21	0.5201057814346157
1107	22-23	0.5402062184405025
1107	24-25	0.509329651891754
1107	26-27	0.5629552388996402
1107	28-29	0.5695362456185151
1107	30-31	0.6048578698518057
1107	32-33	0.5638875586417456
1107	34-35	0.5843452613731159
1107	36-37	0.5849245580450457
1107	38-39	0.5786372115047982
1107	40-41	0.6715254095454455
1107	42-43	0.6049859760065956
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2311	24-25	-0.29383668785045813
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2312	78-79	-0.18125117965490034
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2312	82-83	-0.1976389294098304
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2312	86-87	-0.09777157366331224
2312	88-89	-0.13143818690634745
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2312	100	-0.06892638822014518
2313	1	-0.3146653340748067
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2313	24-25	-0.3842467589232825
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2313	30-31	-0.374740366612464
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2313	34-35	-0.44263513363619467
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2313	100	-0.15462976213925117
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2314	4	-0.39555935515105034
2314	5	-0.28905120528050077
2314	6	-0.3154881251107824
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2314	84-85	-0.44008278963119807
2314	86-87	-0.37100565589067713
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2314	90-91	-0.3314923310001774
2314	92-93	-0.2808422741709151
2314	94-95	-0.2512399622126438
2314	96-97	-0.2785375307532334
2314	98-99	-0.30458448300948504
2314	100	-0.293481072451268
2315	1	-0.4397521704977372
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2315	3	-0.45938768682274045
2315	4	-0.38497503779040443
2315	5	-0.3249257716938345
2315	6	-0.3777205466569029
2315	7	-0.3484368688281734
2315	8	-0.4438027545129728
2315	9	-0.4707621413557206
2315	10-11	-0.5035225606527831
2315	12-13	-0.5203788885958147
2315	14-15	-0.5724487168078838
2315	16-17	-0.6102808934978015
2315	18-19	-0.6237380760245657
2315	20-21	-0.6294687693561869
2315	22-23	-0.6403016477901176
2315	24-25	-0.6282612309887767
2315	26-27	-0.6341625242698541
2315	28-29	-0.618145290330844
2315	30-31	-0.5843467431450833
2315	32-33	-0.6135728749538032
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2315	54-55	-0.6139086841118555
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2315	64-65	-0.6484159305473014
2315	66-67	-0.6038376215956802
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2315	82-83	-0.47389089736396883
2315	84-85	-0.4385115246459357
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2315	88-89	-0.4814877693638131
2315	90-91	-0.5295416137279858
2315	92-93	-0.6474238621014585
2315	94-95	-0.4981124760031044
2315	96-97	-0.4515276936717818
2315	98-99	-0.3958520541528543
2315	100	-0.3875464922513743
2316	1	-0.642636062572219
2316	2	-0.5552658678545761
2316	3	-0.5954269336291276
2316	4	-0.5232009432318705
2316	5	-0.4921649808869617
2316	6	-0.5342541639095657
2316	7	-0.5431257411597485
2316	8	-0.49934095394974065
2316	9	-0.5834376660812737
2316	10-11	-0.6075717063287627
2316	12-13	-0.5901478887941849
2316	14-15	-0.8144085948933935
2316	16-17	-0.9088588881252093
2316	18-19	-0.858390001050239
2316	20-21	-0.7743678242810077
2316	22-23	-0.783694386727781
2316	24-25	-0.7438988477832424
2316	26-27	-0.7563512463945727
2316	28-29	-0.7503871386513552
2316	30-31	-0.7137566338580257
2316	32-33	-0.8280821564461363
2316	34-35	-0.8102536797991178
2316	36-37	-0.8069169788036632
2316	38-39	-0.7271697342047361
2316	40-41	-0.9288509304679664
2316	42-43	-0.8134355685232109
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2316	48-49	-0.8727964618301982
2316	50-51	-0.7806056471673557
2316	52-53	-0.780121523661478
2316	54-55	-0.7942187430462582
2316	56-57	-0.812040130302826
2316	58-59	-0.8856699309419227
2316	60-61	-1.3158911312183434
2316	62-63	-1.2033431738779115
2316	64-65	-0.9276138292857041
2316	66-67	-0.7672076249855166
2316	68-69	-0.7127944536354747
2316	70-71	-0.673712210471912
2316	72-73	-0.7298169126774852
2316	74-75	-0.6548925259146543
2316	76-77	-0.6553666367144757
2316	78-79	-0.6359882782022268
2316	80-81	-0.6463077584125188
2316	82-83	-0.6260023909998331
2316	84-85	-0.6328781258692118
2316	86-87	-0.6355986328943146
2316	88-89	-0.6320315241492445
2316	90-91	-0.6389875684437136
2316	92-93	-0.6384870728064129
2316	94-95	-0.5684996741291677
2316	96-97	-0.7035570155938125
2316	98-99	-0.6753810508590412
2316	100	-0.5867512480504118
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	257902.0
3	40974.0
4	27296.0
5	26579.0
6	30351.0
7	31790.0
8	34106.0
9	41944.0
10	51940.0
11	63203.0
12	71908.0
13	73428.0
14	70143.0
15	67092.0
16	74805.0
17	93029.0
18	124217.0
19	186814.0
20	351934.0
21	431847.0
22	188442.0
23	152068.0
24	168996.0
25	190992.0
26	208435.0
27	242608.0
28	287891.0
29	347540.0
30	426303.0
31	536437.0
32	684431.0
33	900968.0
34	1268992.0
35	2028071.0
36	3621965.0
37	6786945.0
38	7699470.0
39	2183814.0
40	7435.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.523430455402668	32.68670551904094	25.967630927270164	23.822233098286222
2	22.82417789395653	30.531455721046136	26.566311519533937	20.0780548654634
3	25.559171675371406	25.207901020388817	25.69534879776119	23.53757850647858
4	21.279165185038803	26.469610909627473	25.129586892693595	27.121637012640125
5	23.05075235323197	29.963730598417655	25.139252490347392	21.846264558002986
6	27.24777469943692	25.47459246222758	25.50870549573582	21.76892734259968
7	22.540449849069926	25.660029728383776	29.82182040471033	21.977700017835964
8	26.523112768610513	25.492344969859797	25.54688536177561	22.437656899754074
9	26.348147669073736	24.946483313521956	26.201262418282845	22.504106599121464
10-11	22.07332218972725	28.073892107356478	27.634882114392408	22.217903588523864
12-13	22.11380351029513	25.87189606644661	27.3617279191577	24.652572504100554
14-15	22.1331222698396	30.41137773425508	25.13035726305808	22.32514273284724
16-17	24.622419112064403	25.66404469020391	25.128068882329814	24.58546731540188
18-19	22.32396260211475	28.07410557804092	25.105036395967744	24.49689542387658
20-21	24.643310477330424	28.10316759528351	25.075604314762103	22.17791761262397
22-23	24.537578002842242	25.86068313156949	27.414132782588464	22.187606082999807
24-25	22.313077900839193	27.918819041015	25.08804233316486	24.68006072498095
26-27	22.381631139217813	25.695252661968286	29.69738854504534	22.22572765376856
28-29	22.472561687974675	25.766434373341173	29.587866152496506	22.173137786187645
30-31	22.395438135209737	25.71993870161073	29.600995308932294	22.283627854247243
32-33	22.416150146499785	25.779402897182745	29.542169192936797	22.262277763380673
34-35	22.409125811761722	25.86974309936582	29.49996206810358	22.22116902076887
36-37	22.368563458285248	25.833375362931825	29.51023949214263	22.287821686640296
38-39	22.42103682617029	25.7995411303185	29.485646877737576	22.29377516577363
40-41	22.41884884200309	25.91379904641325	29.453119811832472	22.214232299751195
42-43	22.500299308940473	25.902419576357634	29.379275118270098	22.218005996431796
44-45	22.588484180783766	25.94344305547338	29.306409047526678	22.161663716216175
46-47	22.56020085594139	26.09317284210358	29.242512510219896	22.104113791735134
48-49	22.68715773191602	26.073003177263416	29.08743140740841	22.152407683412154
50-51	22.745454229821586	26.12272508848969	29.01978158159812	22.112039100090605
52-53	22.84948544269028	26.266303256836785	28.82414693417777	22.060064366295162
54-55	23.167675850647072	26.423091577536546	28.18246369095118	22.226768880865194
56-57	23.583175978170495	26.807243937223596	27.32700203940814	22.28257804519777
58-59	23.773182367846648	27.15913769306076	26.8814052887791	22.186274650313496
60-61	23.936378126417914	27.381613022721474	26.592573470835536	22.089435380025076
62-63	24.107384891341145	27.469488211289672	26.44892317864626	21.974203718722922
64-65	24.231721566603973	27.54595615772566	26.402180462391183	21.820141813279182
66-67	24.393041633034432	27.37870687610477	26.459374900534925	21.768876590325874
68-69	24.555519132343264	27.193169869219176	26.630597976353847	21.620713022083716
70-71	24.74447038401772	27.101597288341246	26.74615663197553	21.4077756956655
72-73	24.94151790580105	27.03476040917691	26.680983188969638	21.342738496052398
74-75	25.206384512468944	27.054010632620212	26.54490911045074	21.194695744460105
76-77	25.500135944488058	27.151432176013667	26.254939011342465	21.09349286815581
78-79	25.793805216423593	27.182207659723268	25.99159003049619	21.032397093356952
80-81	26.045245181572028	27.25022504270649	25.78075411562365	20.92377566009784
82-83	26.20544299245733	27.36706135787411	25.601263727519708	20.82623192214885
84-85	26.339122078039207	27.373792490991512	25.461049801127118	20.826035629842167
86-87	26.510012540058426	27.367941958633207	25.39284820541592	20.729197295892448
88-89	26.639670670080356	27.366801073004897	25.336137086916168	20.657391169998576
90-91	26.766285574019783	27.31673207276491	25.25594340313915	20.661038950076158
92-93	26.851095241668098	27.281446122559394	25.241286123566454	20.626172512206047
94-95	26.988941277191074	27.3110216245403	25.16762571197412	20.532411386294505
96-97	27.059742478830778	27.32589647114983	25.09662764120233	20.51773340881707
98-99	27.19101356595983	27.331365336879927	25.04644330715954	20.431177790000703
100	27.235868949712643	27.425401788611133	24.98255891073437	20.35617035094186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48233.0
1	29825.0
2	9537.5
3	7423.0
4	7346.5
5	7610.5
6	8175.5
7	8828.5
8	9242.0
9	9858.0
10	11052.5
11	16246.0
12	107401.5
13	125191.5
14	53281.0
15	52655.0
16	57780.0
17	63119.0
18	68069.5
19	73591.5
20	81051.0
21	92214.0
22	107101.0
23	124279.0
24	143029.0
25	164228.0
26	188938.5
27	219332.0
28	252511.0
29	285018.5
30	321851.5
31	361562.5
32	401122.0
33	441274.0
34	482494.5
35	528507.0
36	581718.5
37	627737.0
38	666172.0
39	705908.5
40	738264.0
41	771587.0
42	814267.0
43	857122.0
44	904753.0
45	957801.0
46	1003583.0
47	1037916.0
48	1067050.0
49	1093716.5
50	1117417.5
51	1131923.0
52	1136046.5
53	1129509.0
54	1100606.0
55	1054767.0
56	990120.0
57	910458.0
58	830388.0
59	735413.0
60	618425.5
61	509042.0
62	417406.5
63	330885.0
64	260523.5
65	206545.0
66	158616.5
67	124352.0
68	100560.0
69	80848.0
70	68262.5
71	61873.0
72	55959.5
73	45063.5
74	33038.5
75	24948.5
76	19500.0
77	14900.5
78	11111.5
79	8106.0
80	5740.5
81	4260.0
82	3113.0
83	2241.5
84	1702.0
85	1358.0
86	1023.0
87	777.0
88	659.0
89	580.5
90	489.5
91	405.5
92	341.0
93	285.0
94	252.5
95	210.0
96	164.0
97	133.5
98	109.0
99	83.0
100	68.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022820117803664216
2	0.038018017089658795
3	0.05689239857388391
4	0.13341707912132075
5	0.1783725449882916
6	0.10971939233001382
7	0.12323196026473995
8	0.1489773080272133
9	0.13497941784932108
10-11	0.1335201269948697
12-13	0.1336896573674825
14-15	0.14665706880988516
16-17	0.13716669206852153
18-19	0.1403063280868115
20-21	0.14949088533248148
22-23	0.1584311193940918
24-25	0.1552432835639805
26-27	0.14628642887760424
28-29	0.1648682873659484
30-31	0.15758180546855122
32-33	0.15357789696243124
34-35	0.14102267701422444
36-37	0.1487545916553494
38-39	0.1443384916550336
40-41	0.13891518179390058
42-43	0.14318169617132273
44-45	0.13656336338951713
46-47	0.14514791608113592
48-49	0.1385711348612452
50-51	0.14113735932510957
52-53	0.15637847223549564
54-55	0.15828485789615135
56-57	0.15277345872375872
58-59	0.15084380418843069
60-61	0.15149367061677976
62-63	0.14564321069916153
64-65	0.1449484685839444
66-67	0.15768651540457673
68-69	0.15220669541923948
70-71	0.14503821995768057
72-73	0.14909032827562183
74-75	0.14857342684540042
76-77	0.11326955778002304
78-79	0.03161907655476388
80-81	0.040374821681472044
82-83	0.1663674677198381
84-85	0.04368897425980463
86-87	0.18261246636608822
88-89	0.04064241374020401
90-91	0.17416254073507373
92-93	0.183378677167799
94-95	0.2402361724296744
96-97	0.26650673193475205
98-99	0.2940886587338641
100	0.3110682889947697
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.0083105E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.18591290235675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.544972513626895	16.26836082975611
2	17.596625081003516	11.327268844652108
3	10.64748322007343	10.280969026517651
4	6.338509261097455	8.160428280338571
5	4.012589501199834	6.457442809926449
6	2.7214591110822117	5.255558754997036
7	1.8051900333932014	4.067118243089722
8	1.343158776595482	3.458463311803058
9	0.9911481806411777	2.8710908113901668
>10	3.8018071673406117	19.80327862276876
>50	0.12224409878040252	2.6909984878057402
>100	0.0729849132535826	4.353823039874977
>500	9.038423403429696E-4	0.1877995604631522
>1k	8.416882045244318E-4	0.5197666864149064
>5k	3.0979262756733576E-5	0.081469378484845
>10k+	5.163210459412963E-5	4.216163311716874
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1167437	3.880706462979802	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35109	0.11670670298162374	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32432	0.10780802048192832	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04443690237427287	0.0	0.0	0.0	3.3241249531921654E-6
2	0.04444022649922606	6.648249906384331E-6	0.0	0.0	3.3241249531921654E-6
3	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	3.3241249531921654E-6
4	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	3.3241249531921654E-6
5	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	6.648249906384331E-6
6	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	6.648249906384331E-6
7	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	6.648249906384331E-6
8	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	1.994474971915299E-5
9	0.04444022649922606	6.648249906384331E-6	0.0	3.3241249531921654E-6	1.994474971915299E-5
10-11	0.04445352299903883	9.972374859576496E-6	0.0	3.3241249531921654E-6	1.994474971915299E-5
12-13	0.04449839868590692	1.3296499812768661E-5	0.0	3.3241249531921654E-6	1.994474971915299E-5
14-15	0.0445499226226814	1.662062476596083E-5	0.0	6.648249906384331E-6	1.994474971915299E-5
16-17	0.044609756871838865	1.994474971915299E-5	0.0	1.3296499812768663E-5	1.994474971915299E-5
18-19	0.04468288762080909	1.994474971915299E-5	0.0	1.9944749719152995E-5	1.994474971915299E-5
20-21	0.04475601836977931	2.3268874672345157E-5	4.986187429788248E-6	2.3268874672345157E-5	1.994474971915299E-5
22-23	0.045021948366034695	2.3268874672345157E-5	9.972374859576496E-6	2.3268874672345157E-5	1.994474971915299E-5
24-25	0.04605076503904766	2.9917124578729492E-5	9.972374859576496E-6	2.3268874672345157E-5	1.994474971915299E-5
26-27	0.04969733011269947	3.6565374485113824E-5	9.972374859576496E-6	2.3268874672345157E-5	1.994474971915299E-5
28-29	0.06008854471637819	3.6565374485113824E-5	9.972374859576496E-6	2.3268874672345157E-5	2.493093714894124E-5
30-31	0.08378124532025534	4.653774934469032E-5	9.972374859576496E-6	3.324124953192166E-5	2.6592999625537323E-5
32-33	0.12895610343413688	5.651012420426681E-5	9.972374859576496E-6	3.324124953192166E-5	2.6592999625537323E-5
34-35	0.20534449485849282	5.651012420426681E-5	9.972374859576496E-6	3.324124953192166E-5	2.6592999625537323E-5
36-37	0.31959134537475437	5.651012420426681E-5	9.972374859576496E-6	3.988949943830598E-5	2.6592999625537323E-5
38-39	0.4771282751564374	5.651012420426681E-5	9.972374859576496E-6	3.988949943830598E-5	2.6592999625537323E-5
40-41	0.6819026825854579	5.651012420426681E-5	9.972374859576496E-6	3.988949943830598E-5	2.6592999625537323E-5
42-43	0.934945046397305	5.651012420426681E-5	9.972374859576496E-6	4.155156191490207E-5	2.8255062102133406E-5
44-45	1.2399119040404905	6.648249906384332E-5	9.972374859576496E-6	4.6537749344690314E-5	2.991712457872949E-5
46-47	1.598289139369091	6.648249906384332E-5	9.972374859576496E-6	4.986187429788248E-5	2.991712457872949E-5
48-49	2.0069138474901447	7.313074897022765E-5	9.972374859576496E-6	5.3185999251074646E-5	2.991712457872949E-5
50-51	2.4661001582117272	7.313074897022765E-5	9.972374859576496E-6	5.3185999251074646E-5	2.991712457872949E-5
52-53	2.96987628105543	7.313074897022765E-5	9.972374859576496E-6	5.3185999251074646E-5	2.991712457872949E-5
54-55	3.524195723812419	7.313074897022765E-5	9.972374859576496E-6	5.3185999251074646E-5	2.991712457872949E-5
56-57	4.123362598375401	7.313074897022765E-5	9.972374859576496E-6	5.3185999251074646E-5	2.991712457872949E-5
58-59	4.763268286302228	7.313074897022765E-5	9.972374859576496E-6	5.3185999251074646E-5	2.991712457872949E-5
60-61	5.4371681380628765	7.313074897022765E-5	1.1634437336172579E-5	5.3185999251074646E-5	2.991712457872949E-5
62-63	6.145022264157905	7.313074897022765E-5	1.3296499812768661E-5	5.3185999251074646E-5	2.991712457872949E-5
64-65	6.884576907869052	7.313074897022765E-5	1.3296499812768661E-5	6.64824990638433E-5	2.991712457872949E-5
66-67	7.647712893998143	7.313074897022765E-5	1.3296499812768661E-5	7.977899887661197E-5	2.991712457872949E-5
68-69	8.43452329804387	7.313074897022765E-5	1.3296499812768661E-5	8.310312382980414E-5	2.991712457872949E-5
70-71	9.246708409919787	7.313074897022765E-5	1.3296499812768661E-5	8.808931125959239E-5	2.991712457872949E-5
72-73	10.078776775203224	7.313074897022765E-5	1.3296499812768661E-5	9.141343621278455E-5	2.991712457872949E-5
74-75	10.934104042784147	7.479281144682373E-5	1.3296499812768661E-5	9.307549868938063E-5	2.991712457872949E-5
76-77	11.804482948153126	7.64548739234198E-5	1.3296499812768661E-5	9.307549868938063E-5	2.991712457872949E-5
78-79	12.69267417708378	7.977899887661197E-5	1.3296499812768661E-5	9.307549868938063E-5	3.324124953192166E-5
80-81	13.592551367287387	7.977899887661197E-5	1.3296499812768661E-5	1.0138581107236105E-4	3.6565374485113824E-5
82-83	14.504131139388704	7.977899887661197E-5	1.3296499812768661E-5	1.0969612345534146E-4	3.6565374485113824E-5
84-85	15.426043953907019	8.310312382980414E-5	1.3296499812768661E-5	1.0969612345534146E-4	3.6565374485113824E-5
86-87	16.360159631128504	8.64272487829963E-5	1.4958562289364746E-5	1.0969612345534146E-4	3.6565374485113824E-5
88	17.067805999413956	1.0304787354895713E-4	1.662062476596083E-5	1.1966849831491795E-4	3.6565374485113824E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	171540	0.0	76.85028	1
GTGGTAT	172965	0.0	74.91336	6
AGTGGTA	173925	0.0	74.617355	5
GGTATCA	173485	0.0	74.58646	8
TGGTATC	174695	0.0	73.96701	7
GTATCAA	175340	0.0	73.54111	9
GCAGTGG	181640	0.0	72.20352	3
CAGTGGT	181825	0.0	71.636894	4
AGCAGTG	184415	0.0	71.55798	2
AGTACTT	157675	0.0	41.35458	20-21
CAACGCA	169600	0.0	40.401142	12-13
GAGTACT	152395	0.0	40.129463	20-21
ATCAACG	171270	0.0	40.11741	10-11
ACGCAGA	170795	0.0	40.038826	14-15
AGAGTAC	169895	0.0	39.685787	18-19
GTACTTT	156850	0.0	38.885715	22-23
TACTTTT	169695	0.0	38.47882	22-23
TCAACGC	169360	0.0	38.053116	12-13
AACGCAG	169995	0.0	37.68031	14-15
GCAGAGT	181550	0.0	37.417496	16-17
>>END_MODULE