FastQCFastQC Report
Thu 26 May 2016
SRR1660585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30083105
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11212003.727008897519056No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT329500.10952991720768186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316330.10515204464432777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1680400.075.275861
GTGGTAT1691800.073.8726356
AGTGGTA1705350.073.459245
GGTATCA1694850.073.45698
TGGTATC1710950.072.738217
GTATCAA1713200.072.5055549
GCAGTGG1798600.070.2881243
CAGTGGT1792150.070.184534
AGCAGTG1812400.070.0970842
AGTACTT1548550.040.95677620-21
CAACGCA1657050.039.90709312-13
GAGTACT1488550.039.9000720-21
ACGCAGA1674900.039.52106514-15
ATCAACG1671600.039.5147610-11
AGAGTAC1675750.039.07082418-19
GTACTTT1526800.038.76914622-23
TACTTTT1661500.038.22030322-23
TCAACGC1654250.037.44950512-13
AACGCAG1660300.037.223914-15
GCAGAGT1786800.036.9460916-17
Produced by FastQC (version 0.11.5)