##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30083105
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42897666314697	34.0	31.0	34.0	31.0	34.0
2	32.71921349209133	34.0	31.0	34.0	31.0	34.0
3	32.80870235967996	34.0	31.0	34.0	31.0	34.0
4	36.240876698066906	37.0	37.0	37.0	35.0	37.0
5	36.16509190125155	37.0	37.0	37.0	35.0	37.0
6	36.149353133594424	37.0	37.0	37.0	35.0	37.0
7	36.094966659857754	37.0	36.0	37.0	35.0	37.0
8	36.11749847630423	37.0	36.0	37.0	35.0	37.0
9	37.871280873433776	39.0	38.0	39.0	35.0	39.0
10-11	37.80218848752481	39.0	38.0	39.0	35.0	39.0
12-13	37.83238267791839	39.0	38.0	39.0	35.0	39.0
14-15	39.31868711690498	41.0	39.0	41.0	36.0	41.0
16-17	39.30372418339131	41.0	39.0	41.0	36.0	41.0
18-19	39.285230580420475	41.0	39.0	41.0	36.0	41.0
20-21	39.23217890905876	41.0	39.0	41.0	36.0	41.0
22-23	39.110919966539356	40.5	39.0	41.0	35.5	41.0
24-25	39.11492405787235	41.0	39.0	41.0	35.0	41.0
26-27	39.0692907863068	41.0	39.0	41.0	35.0	41.0
28-29	38.983978249585604	41.0	39.0	41.0	35.0	41.0
30-31	38.86937728003808	40.0	38.5	41.0	35.0	41.0
32-33	38.7874370680819	40.0	38.0	41.0	35.0	41.0
34-35	38.62901425900019	40.0	38.0	41.0	34.5	41.0
36-37	38.48113976266745	40.0	38.0	41.0	34.0	41.0
38-39	38.17924248510917	40.0	37.5	41.0	34.0	41.0
40-41	38.03568155614256	40.0	37.0	41.0	33.0	41.0
42-43	37.906464741588344	40.0	37.0	41.0	33.0	41.0
44-45	37.78180975667239	40.0	37.0	41.0	33.0	41.0
46-47	37.61385089737246	40.0	36.0	41.0	33.0	41.0
48-49	37.424064587083016	40.0	36.0	41.0	33.0	41.0
50-51	37.210985601386554	40.0	35.5	41.0	32.5	41.0
52-53	36.85040643244771	39.0	35.0	41.0	31.5	41.0
54-55	36.288877128873494	39.0	35.0	41.0	30.5	41.0
56-57	35.790363461484446	39.0	35.0	41.0	29.0	41.0
58-59	35.68883482938347	39.0	35.0	41.0	29.0	41.0
60-61	35.45023447546389	38.5	35.0	41.0	29.0	41.0
62-63	35.11954866361036	37.5	35.0	40.0	28.0	41.0
64-65	34.99375184177298	37.0	35.0	40.0	29.0	41.0
66-67	34.737229451547634	37.0	35.0	40.0	29.0	41.0
68-69	34.41401348032392	36.0	35.0	39.0	28.0	41.0
70-71	34.055884058510586	36.0	34.0	39.0	28.0	41.0
72-73	33.6933117442498	35.0	34.0	38.5	28.0	40.5
74-75	33.328721004696824	35.0	34.0	37.0	27.0	39.5
76-77	31.773075668219754	34.5	32.0	36.0	25.0	39.0
78-79	32.642661769787395	35.0	33.5	36.0	26.0	39.0
80-81	32.56298156390439	35.0	34.0	36.0	26.0	38.0
82-83	32.32880590284813	35.0	34.0	36.0	26.0	37.0
84-85	32.105893307888266	35.0	34.0	35.0	26.0	37.0
86-87	31.90451545809517	35.0	34.0	35.0	25.5	36.0
88-89	31.72622678077944	35.0	33.5	35.0	25.0	36.0
90-91	31.55351864443514	35.0	33.0	35.0	24.5	36.0
92-93	31.38080946099148	35.0	33.0	35.0	24.0	35.5
94-95	31.242787272125	35.0	33.0	35.0	23.0	35.0
96-97	31.062996439363555	35.0	33.0	35.0	21.5	35.0
98-99	30.905812298963156	35.0	33.0	35.0	18.5	35.0
100	30.803768427494436	35.0	33.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.551583943598601
1101	2	0.41502213296003276
1101	3	0.370154733621483
1101	4	0.23210524149614997
1101	5	0.2656227620012501
1101	6	0.2511744041504116
1101	7	0.26787173834762257
1101	8	0.24678905803742168
1101	9	0.35126748440080036
1101	10-11	0.32191271046441017
1101	12-13	0.3482507825486252
1101	14-15	0.4425689826173951
1101	16-17	0.4224268970537466
1101	18-19	0.3772233198122663
1101	20-21	0.36242598565890205
1101	22-23	0.25858640763463825
1101	24-25	0.3644137583171414
1101	26-27	0.444287543148171
1101	28-29	0.4601075799538279
1101	30-31	0.43049798007564277
1101	32-33	0.4272126688447031
1101	34-35	0.4704940803029132
1101	36-37	0.41107648576520006
1101	38-39	0.3416177094730557
1101	40-41	0.015006870556710794
1101	42-43	0.0896708572984437
1101	44-45	0.3068337642001069
1101	46-47	0.3210173962112677
1101	48-49	0.34148170891538143
1101	50-51	0.37352188305857226
1101	52-53	0.2986495019651727
1101	54-55	0.24737216689652541
1101	56-57	0.23767332904741778
1101	58-59	0.21454940308780834
1101	60-61	0.25054014707143324
1101	62-63	0.20655496117495886
1101	64-65	0.2598989847072275
1101	66-67	0.2520806433370808
1101	68-69	0.2753857200664527
1101	70-71	0.2986643682397627
1101	72-73	0.24931799969389346
1101	74-75	0.20032940523405784
1101	76-77	0.1299885856761236
1101	78-79	0.26935065264044766
1101	80-81	0.26897638032865956
1101	82-83	0.23574323122101504
1101	84-85	0.23144956364252067
1101	86-87	0.1755827946787214
1101	88-89	0.14884960414505954
1101	90-91	0.12640851001119913
1101	92-93	0.010124001516977899
1101	94-95	0.02767723479084694
1101	96-97	-0.0342100700440362
1101	98-99	-0.028062439264846972
1101	100	-0.04147477946184708
1102	1	0.49153488467241147
1102	2	0.35126311794224563
1102	3	0.26313396724513893
1102	4	0.19728428682748955
1102	5	0.22271849440645042
1102	6	0.23273151025610872
1102	7	0.2423442038433521
1102	8	0.24473934034278244
1102	9	0.31569141565937286
1102	10-11	0.2522080978216863
1102	12-13	0.3194845118376506
1102	14-15	0.37488998605063983
1102	16-17	0.33266352858048975
1102	18-19	0.31433487555460005
1102	20-21	0.2786519287544209
1102	22-23	0.3198611258313804
1102	24-25	0.36815901263874906
1102	26-27	0.39337532981728884
1102	28-29	0.3182898738146065
1102	30-31	0.3856753769797976
1102	32-33	0.38352354237508024
1102	34-35	0.46019271106474946
1102	36-37	0.45605797501314527
1102	38-39	0.43792712656161825
1102	40-41	0.4257678838136485
1102	42-43	0.42044674844822083
1102	44-45	0.40648108965475416
1102	46-47	0.37515716587159176
1102	48-49	0.379801820648062
1102	50-51	0.34342799165315085
1102	52-53	0.4002087134597687
1102	54-55	0.4187132522158876
1102	56-57	0.32161599763882265
1102	58-59	0.30570230069277926
1102	60-61	0.29600308604298675
1102	62-63	0.24584110618900468
1102	64-65	0.3258950180155793
1102	66-67	0.33453356704020365
1102	68-69	0.29907715483312103
1102	70-71	0.26583713679946896
1102	72-73	0.21418414537676256
1102	74-75	0.2655135176405352
1102	76-77	0.004769417714019397
1102	78-79	0.2818588792973813
1102	80-81	0.277644419547741
1102	82-83	0.09977558336091619
1102	84-85	0.1955483793845474
1102	86-87	0.2605779051824406
1102	88-89	0.28308659513779233
1102	90-91	0.27118376148153445
1102	92-93	0.23565427903889358
1102	94-95	0.20169792325642177
1102	96-97	0.2610979890629075
1102	98-99	0.2781259322321823
1102	100	0.2928402715363916
1103	1	0.46350712447053155
1103	2	0.33056803397590073
1103	3	0.28259185044809954
1103	4	0.21108599940252049
1103	5	0.19622890388370706
1103	6	0.1889590764063911
1103	7	0.22718296738124621
1103	8	0.22096364237728494
1103	9	0.2508911170500596
1103	10-11	0.24490382230022334
1103	12-13	0.25502818075678846
1103	14-15	0.29592048826366835
1103	16-17	0.33780520770559974
1103	18-19	0.34521320140688516
1103	20-21	0.33005204890602613
1103	22-23	0.3421950814669259
1103	24-25	0.3465867253408135
1103	26-27	0.3047260658976185
1103	28-29	0.31759601838005125
1103	30-31	0.37374972734765777
1103	32-33	0.32344356298910526
1103	34-35	0.41130509231415147
1103	36-37	0.37957129979547943
1103	38-39	0.3547786158764268
1103	40-41	0.32316467453295417
1103	42-43	0.3093271678486289
1103	44-45	0.31132852814887
1103	46-47	0.30849550480940735
1103	48-49	0.3548992845976713
1103	50-51	0.345149654567102
1103	52-53	0.3475183442513341
1103	54-55	0.284920865197428
1103	56-57	0.24818094580580663
1103	58-59	0.243952663673916
1103	60-61	0.2480640443029074
1103	62-63	0.22680333896731497
1103	64-65	0.21979346827872348
1103	66-67	0.1981008369356445
1103	68-69	0.23162343701122978
1103	70-71	0.1558345027657495
1103	72-73	0.22420608796337405
1103	74-75	0.232798861632439
1103	76-77	0.2575157889927375
1103	78-79	0.279511639787799
1103	80-81	0.26250839919224944
1103	82-83	0.2711190063856108
1103	84-85	0.25427801253743354
1103	86-87	0.24332053444052448
1103	88-89	0.23597644046332533
1103	90-91	0.21389213462246204
1103	92-93	0.20304777571993426
1103	94-95	0.20782764287674027
1103	96-97	0.2278787886413305
1103	98-99	0.26157266751367203
1103	100	0.2812821726265007
1104	1	0.39396444662802566
1104	2	0.2735112623341607
1104	3	0.1856050514946972
1104	4	0.16444526865453923
1104	5	0.15104385173339097
1104	6	0.17597529054577166
1104	7	0.15423148172770595
1104	8	0.17091974153844092
1104	9	0.20954928190638356
1104	10-11	0.1630398368306274
1104	12-13	0.1826364573630741
1104	14-15	0.21551475766144534
1104	16-17	0.25825851368917085
1104	18-19	0.25789477579357367
1104	20-21	0.23022405350329223
1104	22-23	0.268349916920684
1104	24-25	0.27454745368775235
1104	26-27	0.23209952303785286
1104	28-29	0.2065931152749414
1104	30-31	0.2769443980260107
1104	32-33	0.25848891074922875
1104	34-35	0.33417105784249657
1104	36-37	0.24471516451040998
1104	38-39	0.2885726904881665
1104	40-41	0.25877781409391787
1104	42-43	0.2676283539471669
1104	44-45	0.1325410517949166
1104	46-47	0.2069562869071504
1104	48-49	0.25132509029544536
1104	50-51	0.25447215417505475
1104	52-53	0.20627137597946898
1104	54-55	0.20364793414149318
1104	56-57	0.04501940976523855
1104	58-59	0.08811574872421346
1104	60-61	0.08870066721389236
1104	62-63	0.01796476706518746
1104	64-65	-7.874743264366657E-4
1104	66-67	0.06138767631943409
1104	68-69	0.04410240432523693
1104	70-71	0.06151487675109735
1104	72-73	0.08157729883701137
1104	74-75	0.10746304738143664
1104	76-77	-0.1900229291332387
1104	78-79	0.0917469541382161
1104	80-81	0.13675351106630984
1104	82-83	0.1401093059382177
1104	84-85	0.1082143862539624
1104	86-87	0.02694293125030356
1104	88-89	0.10027238564736507
1104	90-91	0.1335542330917825
1104	92-93	0.1308394426321975
1104	94-95	0.16927550485155507
1104	96-97	0.2393773840831379
1104	98-99	0.27272504638899875
1104	100	0.27688177564439087
1105	1	0.41613819605758096
1105	2	0.3013372315568006
1105	3	0.220198991312607
1105	4	0.16269091849093087
1105	5	0.16291624280952988
1105	6	0.14389401649884803
1105	7	0.16687068013769846
1105	8	0.1598675587875178
1105	9	0.22484229365255004
1105	10-11	0.20139625864900523
1105	12-13	0.2357040892481308
1105	14-15	0.2736368285232018
1105	16-17	0.2802501437648246
1105	18-19	0.24252257315713877
1105	20-21	0.23372439191791017
1105	22-23	0.24910750767276824
1105	24-25	0.1992535040902368
1105	26-27	0.2571074719840709
1105	28-29	0.22222691121308458
1105	30-31	0.2592048552904984
1105	32-33	0.21676192512037318
1105	34-35	0.17962372051819386
1105	36-37	0.17142908585554295
1105	38-39	0.14285506808895576
1105	40-41	0.15858770048559734
1105	42-43	0.25432605998280167
1105	44-45	0.2544162745483831
1105	46-47	0.2678358480564498
1105	48-49	0.2752067133587772
1105	50-51	0.1958961140958877
1105	52-53	0.18499780274887456
1105	54-55	0.18370094462741093
1105	56-57	0.1609777431519177
1105	58-59	0.11595392868225218
1105	60-61	0.11448960929982377
1105	62-63	0.1543151659837605
1105	64-65	0.10374816980817769
1105	66-67	0.17004861447627917
1105	68-69	0.17141856435821268
1105	70-71	0.14431535108159466
1105	72-73	0.16840833409701617
1105	74-75	0.11971471024417468
1105	76-77	0.001584958353642918
1105	78-79	0.20710780243081217
1105	80-81	0.16892211121677292
1105	82-83	0.19746330995500472
1105	84-85	0.18476678881902586
1105	86-87	0.1903005595460776
1105	88-89	0.1935096616852512
1105	90-91	0.21557860224949366
1105	92-93	0.2443454964916576
1105	94-95	0.2707974415906307
1105	96-97	0.2893806836982904
1105	98-99	0.30792367312092495
1105	100	0.36306397207859575
1106	1	0.43356561692951345
1106	2	0.2824076458994327
1106	3	0.23733755202626838
1106	4	0.16614727166154353
1106	5	0.16670340940403605
1106	6	0.145988344608746
1106	7	0.14618233486561394
1106	8	0.1733923793565637
1106	9	0.19425711968206372
1106	10-11	0.19955430490542625
1106	12-13	0.24506334781867878
1106	14-15	0.2647332358781327
1106	16-17	0.23867068032964056
1106	18-19	0.21635244425232258
1106	20-21	0.28863554192111707
1106	22-23	0.35642699773308806
1106	24-25	0.28881214812140144
1106	26-27	0.28110556083779414
1106	28-29	0.24335813411360618
1106	30-31	0.32810815139959715
1106	32-33	0.2836076094307316
1106	34-35	0.23470201961507087
1106	36-37	0.2824826807949492
1106	38-39	0.22534893239473064
1106	40-41	0.3063496328118873
1106	42-43	0.365059216087829
1106	44-45	0.27691819456781985
1106	46-47	0.34392928463829975
1106	48-49	0.3459396402443673
1106	50-51	0.3720340226106842
1106	52-53	0.41101115126395626
1106	54-55	0.370497317229983
1106	56-57	0.41578710569207544
1106	58-59	0.4108606707907896
1106	60-61	0.44429076029904024
1106	62-63	0.4380844453684887
1106	64-65	0.41889679302516925
1106	66-67	0.4563184740244637
1106	68-69	0.44544955216215243
1106	70-71	0.4512986187324657
1106	72-73	0.46334006485312074
1106	74-75	0.43829872348679544
1106	76-77	-0.34337391977637566
1106	78-79	0.42296859493058747
1106	80-81	0.41018360433473333
1106	82-83	0.4328098193260175
1106	84-85	0.44874980336106063
1106	86-87	0.433060149031089
1106	88-89	0.3976381471250683
1106	90-91	0.45796837703935367
1106	92-93	0.4552279010172704
1106	94-95	0.4803164232264159
1106	96-97	0.546191686630138
1106	98-99	0.5934494388088218
1106	100	0.6378857534965547
1107	1	0.541393168264392
1107	2	0.3954986957263813
1107	3	0.3220054929410878
1107	4	0.2246117936586245
1107	5	0.25813136281882976
1107	6	0.22722092826902696
1107	7	0.19339329240862213
1107	8	0.24459910758407943
1107	9	0.27949546421984195
1107	10-11	0.3148281533670243
1107	12-13	0.347710492005497
1107	14-15	0.37342396962042557
1107	16-17	0.38905061165976207
1107	18-19	0.3934490476373824
1107	20-21	0.39790934148950186
1107	22-23	0.4411412024718615
1107	24-25	0.45870443472740163
1107	26-27	0.46415239222449856
1107	28-29	0.4733271681542064
1107	30-31	0.5018799758498247
1107	32-33	0.4857514111929291
1107	34-35	0.488554462499188
1107	36-37	0.4458083811758726
1107	38-39	0.5015488650670008
1107	40-41	0.5643512963225419
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2314	86-87	-0.34176527602651063
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2314	90-91	-0.3422671905900394
2314	92-93	-0.32577713243028583
2314	94-95	-0.29932094120801267
2314	96-97	-0.27329844128022884
2314	98-99	-0.317520853714516
2314	100	-0.27090466715869965
2315	1	-1.6175622612031972
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2315	3	-0.5949279998152974
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2315	14-15	-0.4956416376331063
2315	16-17	-0.5180565017975383
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2315	26-27	-0.5628226071273019
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2315	96-97	-0.39374682366209157
2315	98-99	-0.4229941011229563
2315	100	-0.4134821813411165
2316	1	-4.4899441439831556
2316	2	-2.482130492583618
2316	3	-1.3260390170086787
2316	4	-0.7598257588931148
2316	5	-0.6785307790488915
2316	6	-0.9297320817297532
2316	7	-0.6585764607250724
2316	8	-0.5354142371549244
2316	9	-0.5439589661699031
2316	10-11	-0.5155254427161893
2316	12-13	-0.45021723383286627
2316	14-15	-0.6505592846027568
2316	16-17	-0.6229264381369646
2316	18-19	-0.6418513504737788
2316	20-21	-0.6183632141105804
2316	22-23	-0.5948359209948464
2316	24-25	-0.629288880305154
2316	26-27	-0.6146669932652813
2316	28-29	-0.6254856629654597
2316	30-31	-0.6079763719892171
2316	32-33	-0.6444719647360628
2316	34-35	-0.6087293078878133
2316	36-37	-0.6463245378629026
2316	38-39	-0.6687285605675797
2316	40-41	-0.6809513768444191
2316	42-43	-0.7037823424560372
2316	44-45	-0.6838638537304931
2316	46-47	-0.6978535048102117
2316	48-49	-0.6627608821201321
2316	50-51	-0.6680899973426122
2316	52-53	-0.6848083809313863
2316	54-55	-0.7191579564464732
2316	56-57	-0.6147970753554759
2316	58-59	-0.5382631113614238
2316	60-61	-0.5390238343435243
2316	62-63	-0.5927264280599545
2316	64-65	-0.5460695781504441
2316	66-67	-0.5039267433070052
2316	68-69	-0.5313717044652009
2316	70-71	-0.5407042478272004
2316	72-73	-0.5025407964048867
2316	74-75	-0.579223490719599
2316	76-77	-0.48386671328369957
2316	78-79	-0.5972256897163319
2316	80-81	-0.5185099918455762
2316	82-83	-0.53117976395475
2316	84-85	-0.49913210915195805
2316	86-87	-0.47938297442273026
2316	88-89	-0.4739995104624377
2316	90-91	-0.5528410258850904
2316	92-93	-0.5222516825327723
2316	94-95	-0.5310209286683332
2316	96-97	-0.4922676989426762
2316	98-99	-0.4922222765404136
2316	100	-0.4944690343995717
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	249.0
3	112.0
4	216.0
5	549.0
6	1330.0
7	3149.0
8	6783.0
9	12234.0
10	20034.0
11	30535.0
12	43406.0
13	52743.0
14	52554.0
15	51871.0
16	58781.0
17	75878.0
18	106467.0
19	167737.0
20	335608.0
21	408075.0
22	169622.0
23	126809.0
24	138377.0
25	158447.0
26	188030.0
27	231829.0
28	285290.0
29	350379.0
30	430288.0
31	530796.0
32	665063.0
33	855530.0
34	1183412.0
35	1903911.0
36	3482905.0
37	6997909.0
38	8586796.0
39	2362449.0
40	6952.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.911666111885342	32.3243129581123	26.221425810940836	23.542595119061524
2	22.959935465092986	30.461027974047074	26.613870516952463	19.965166043907473
3	25.333432170648607	25.45205024547832	25.894710668995106	23.31980691487797
4	21.52406142916431	26.694754414479487	25.25983936830989	26.521344788046314
5	23.071152396004337	29.843594934764877	25.203824538723644	21.881428130507143
6	27.01538288683964	25.60354724022005	25.600848050758056	21.780221822182252
7	22.53920265211985	25.792533716183886	29.687174910967467	21.981088720728795
8	26.279970102820172	25.602510113234654	25.630825009585944	22.48669477435923
9	26.068422790799023	25.06349992794959	26.330470209109063	22.537607072142322
10-11	22.085085631951888	28.079692904040325	27.61149821469559	22.223723249312197
12-13	22.13730597290406	25.96980265168772	27.320412902856933	24.57247847255129
14-15	22.10936171648505	30.321871695092646	25.189103651368434	22.379662937053872
16-17	24.50861538395056	25.789299342604426	25.19737739837693	24.504707875068082
18-19	22.331893599414023	28.071680100840656	25.163838639661694	24.432587660083623
20-21	24.542400127912327	28.057060599296513	25.16395332197258	22.236585950818576
22-23	24.404008495798553	25.984877558350444	27.389720908130993	22.22139303772001
24-25	22.318846076951118	27.903849563873713	25.165623005590977	24.61168135358419
26-27	22.430773529824364	25.738980222737556	29.5556228253442	22.274623422093875
28-29	22.457221187967406	25.868401621085525	29.458876350364253	22.215500840582816
30-31	22.443048246375312	25.814366484436484	29.444274752201665	22.298310516986536
32-33	22.439655306717853	25.87839778786989	29.3648906513916	22.317056254020663
34-35	22.466474482757107	25.942014742929757	29.33141788954296	22.26009288477017
36-37	22.441614059911412	25.9004788895216	29.35481292620264	22.303094124364346
38-39	22.482488094230966	25.881311121308787	29.349493677597444	22.286707106862806
40-41	22.460756627349472	26.03534109926485	29.295938700476565	22.20796357290911
42-43	22.49529262355066	25.97352899576024	29.235531039764677	22.295647340924415
44-45	22.554817729087475	26.041464137428633	29.17936662455554	22.22435150892835
46-47	22.570348040868787	26.190817071575555	29.09357262157613	22.145262265979525
48-49	22.59999424926383	26.25692726864464	28.920792916821586	22.222285565269942
50-51	22.657797458074892	26.30164505957746	28.81020094169136	22.23035654065629
52-53	22.733982025728768	26.452487487569766	28.554029582379787	22.25950090432168
54-55	22.95688801392657	26.66043697158876	27.916490208148343	22.466184806336333
56-57	23.303621942688025	27.015622998379612	27.10926807023808	22.571486988694282
58-59	23.357402489983286	27.39495619452291	26.638138431643277	22.60950288385053
60-61	23.418711960573265	27.60777093391177	26.348351065248625	22.62516604026634
62-63	23.390667166200544	27.747956327996366	26.22270036302333	22.638676142779765
64-65	23.343162492297708	27.889038677471547	26.10614936546486	22.661649464765887
66-67	23.29725277467678	27.810534543175464	26.153085593892534	22.73912708825522
68-69	23.228541402225602	27.733766178723908	26.25366131587813	22.78403110317236
70-71	23.186245236321184	27.708344933144367	26.3148584562664	22.79055137426805
72-73	23.16321104990708	27.66909827191378	26.24962244137497	22.91806823680417
74-75	23.22218238257605	27.72472390103034	26.084312757876322	22.968780958517286
76-77	23.23823953677654	27.881089734586904	25.86614480120985	23.014525927426707
78-79	23.34194684685637	27.97625280382962	25.568595938126037	23.11320441118797
80-81	23.393106475795207	28.08439811367156	25.339295719781358	23.183199690751877
82-83	23.398776392811328	28.212747030561914	25.137987940178096	23.25048863644866
84-85	23.383843324050716	28.29851390212386	24.96048521360568	23.357157560219743
86-87	23.390074801003628	28.35515888511208	24.840821859779417	23.413944454104875
88-89	23.37891697524181	28.43650935404271	24.723697606667553	23.460876064047927
90-91	23.391522569434485	28.422165131732797	24.623835830785918	23.562476468046796
92-93	23.44225327981735	28.419581599558025	24.57623702879497	23.56192809182965
94-95	23.48854614575191	28.394741832666544	24.551863911654067	23.564848109927482
96-97	23.49259825406985	28.35123236115421	24.516395498403508	23.639773886372435
98-99	23.46716863818834	28.290264380875946	24.561491685922153	23.681075295013564
100	23.451818213155644	28.298483010997643	24.557087391220826	23.692611384625888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47336.0
1	29393.5
2	9203.0
3	6726.0
4	6519.5
5	6610.5
6	6785.0
7	7108.0
8	7177.5
9	7204.5
10	7815.5
11	12274.0
12	101731.5
13	118001.0
14	46163.0
15	45554.0
16	51020.5
17	57593.5
18	64015.0
19	70836.0
20	79431.5
21	92111.0
22	108415.0
23	126917.0
24	146499.5
25	169526.0
26	196008.0
27	227710.0
28	262830.5
29	297402.5
30	336305.0
31	378326.0
32	419365.5
33	459020.0
34	497616.0
35	541162.5
36	593201.0
37	636012.5
38	670112.5
39	705084.5
40	735654.0
41	768388.5
42	809162.0
43	854690.5
44	905162.5
45	955626.0
46	996262.0
47	1025094.5
48	1054656.0
49	1087388.5
50	1120023.0
51	1144295.0
52	1150054.0
53	1140753.0
54	1108960.5
55	1059969.5
56	992495.5
57	909762.0
58	826031.5
59	728401.5
60	609222.5
61	498858.5
62	409167.5
63	323376.0
64	253799.5
65	201366.5
66	154397.5
67	121106.5
68	98195.5
69	78296.5
70	64848.5
71	58829.0
72	53612.0
73	42617.5
74	30881.0
75	23170.0
76	17800.5
77	13127.0
78	9520.5
79	6585.5
80	4240.5
81	2986.0
82	2015.0
83	1258.0
84	815.0
85	563.0
86	345.0
87	203.5
88	151.5
89	124.0
90	91.0
91	82.5
92	68.0
93	37.0
94	26.0
95	19.0
96	9.5
97	4.0
98	1.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02723123161655022
2	0.01083664734740646
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007110303274878042
26-27	0.028314896351290867
28-29	0.032985291910525856
30-31	0.030093303201248676
32-33	0.03687950429319048
34-35	0.0297858216430784
36-37	2.3435080920004766E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0057125087320607365
54-55	0.033031829659870546
56-57	0.045597021982936935
58-59	0.05063473334949966
60-61	0.055190446597849524
62-63	0.03679972529431386
64-65	0.0011119197968427793
66-67	2.1606812195749078E-5
68-69	0.0
70-71	0.0
72-73	9.473756116597672E-5
74-75	2.1606812195749078E-5
76-77	0.0
78-79	4.753498683064797E-4
80-81	0.01659403176633529
82-83	0.04048784192988057
84-85	0.05488795122710904
86-87	0.05894670779495667
88-89	0.05815556605609694
90-91	0.036440719799369116
92-93	0.0024980799023239125
94-95	0.0
96-97	0.0
98-99	4.105294317192325E-4
100	8.642724878299631E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.0083105E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.020795880498113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.90262441873526	14.859775342337509
2	18.450471087055885	11.446965949811853
3	11.17952590129113	10.403933730740816
4	6.613963570525448	8.206816555292818
5	4.311443719964301	6.687220779363402
6	2.8403374410366493	5.286571679408055
7	2.0012958795719404	4.345725368367817
8	1.3865391434974033	3.4409238200602905
9	1.0109904655374913	2.822555598171145
>10	4.095016571722469	20.51747911625807
>50	0.12768055103316608	2.6958404842558013
>100	0.07817996872285066	4.467452717895875
>500	0.001037087746204324	0.20544011523402306
>1k	8.085243688062708E-4	0.47497192362651214
>5k	3.21259466098886E-5	0.07915442638540868
>10k+	5.354324434848508E-5	4.059172392790545
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1121200	3.727008897519056	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32950	0.10952991720768186	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31633	0.10515204464432777	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04453330199791544	0.0	0.0	0.0	0.0
2	0.044536626122868635	0.0	0.0	0.0	0.0
3	0.04454327437277502	0.0	0.0	0.0	0.0
4	0.04454327437277502	0.0	0.0	0.0	0.0
5	0.04454327437277502	0.0	0.0	0.0	0.0
6	0.04454327437277502	3.3241249531921654E-6	0.0	0.0	0.0
7	0.04454327437277502	3.3241249531921654E-6	0.0	0.0	0.0
8	0.04454327437277502	3.3241249531921654E-6	0.0	0.0	0.0
9	0.04454659849772821	3.3241249531921654E-6	0.0	0.0	0.0
10-11	0.04455989499754098	8.310312382980413E-6	0.0	0.0	0.0
12-13	0.04460144655945589	1.3296499812768661E-5	0.0	0.0	0.0
14-15	0.04465629462118355	1.3296499812768661E-5	3.3241249531921654E-6	0.0	0.0
16-17	0.044736073620060164	1.3296499812768661E-5	3.3241249531921654E-6	3.3241249531921654E-6	3.3241249531921654E-6
18-19	0.04482250086884316	1.662062476596083E-5	9.972374859576496E-6	3.3241249531921654E-6	8.310312382980413E-6
20-21	0.044902279867719774	1.662062476596083E-5	9.972374859576496E-6	3.3241249531921654E-6	9.972374859576496E-6
22-23	0.045186492551217705	1.662062476596083E-5	9.972374859576496E-6	6.648249906384331E-6	9.972374859576496E-6
24-25	0.04624190222385621	1.662062476596083E-5	9.972374859576496E-6	9.972374859576496E-6	9.972374859576496E-6
26-27	0.04992669473446973	2.1606812195749074E-5	9.972374859576496E-6	1.3296499812768661E-5	1.828268724255691E-5
28-29	0.06038605389968888	2.6592999625537323E-5	9.972374859576496E-6	1.3296499812768661E-5	2.3268874672345157E-5
30-31	0.08430978118781289	3.6565374485113824E-5	9.972374859576496E-6	1.3296499812768661E-5	2.493093714894124E-5
32-33	0.12957771480038383	3.6565374485113824E-5	9.972374859576496E-6	1.4958562289364746E-5	2.6592999625537323E-5
34-35	0.20622372590861215	3.988949943830598E-5	9.972374859576496E-6	1.662062476596083E-5	2.6592999625537323E-5
36-37	0.3207564511708482	4.155156191490207E-5	9.972374859576496E-6	1.662062476596083E-5	2.6592999625537323E-5
38-39	0.4785626350737399	4.986187429788248E-5	9.972374859576496E-6	1.662062476596083E-5	2.6592999625537323E-5
40-41	0.6838954954948966	4.986187429788248E-5	9.972374859576496E-6	1.828268724255691E-5	2.6592999625537323E-5
42-43	0.9378237386067694	4.986187429788248E-5	9.972374859576496E-6	1.994474971915299E-5	2.6592999625537323E-5
44-45	1.2437230132993253	4.986187429788248E-5	9.972374859576496E-6	1.994474971915299E-5	2.6592999625537323E-5
46-47	1.6024476196855344	4.986187429788248E-5	9.972374859576496E-6	1.994474971915299E-5	2.6592999625537323E-5
48-49	2.012167626978665	4.986187429788248E-5	9.972374859576496E-6	2.1606812195749074E-5	2.6592999625537323E-5
50-51	2.4730891309258136	4.986187429788248E-5	9.972374859576496E-6	2.3268874672345157E-5	2.6592999625537323E-5
52-53	2.9779156772547246	5.3185999251074646E-5	9.972374859576496E-6	2.3268874672345157E-5	2.6592999625537323E-5
54-55	3.533257288434821	5.3185999251074646E-5	9.972374859576496E-6	2.3268874672345157E-5	2.6592999625537323E-5
56-57	4.135038587273488	5.3185999251074646E-5	9.972374859576496E-6	2.3268874672345157E-5	2.6592999625537323E-5
58-59	4.777937649720665	5.3185999251074646E-5	9.972374859576496E-6	3.157918705532557E-5	2.6592999625537323E-5
60-61	5.454586552817603	5.3185999251074646E-5	9.972374859576496E-6	4.155156191490207E-5	2.6592999625537323E-5
62-63	6.164885572815705	5.651012420426681E-5	9.972374859576496E-6	4.6537749344690314E-5	2.6592999625537323E-5
64-65	6.907405335985098	6.149631163405506E-5	9.972374859576496E-6	5.484806172767073E-5	2.8255062102133406E-5
66-67	7.6741479976884035	6.648249906384332E-5	9.972374859576496E-6	6.980662401703547E-5	2.991712457872949E-5
68-69	8.464649842494648	6.648249906384332E-5	9.972374859576496E-6	7.64548739234198E-5	3.324124953192166E-5
70-71	9.280431657569922	6.648249906384332E-5	9.972374859576496E-6	7.64548739234198E-5	4.155156191490207E-5
72-73	10.118938852887693	6.648249906384332E-5	9.972374859576496E-6	8.310312382980414E-5	4.6537749344690314E-5
74-75	10.980935977187194	6.980662401703547E-5	9.972374859576496E-6	8.64272487829963E-5	4.6537749344690314E-5
76-77	11.858950397573654	7.479281144682373E-5	9.972374859576496E-6	9.307549868938063E-5	4.6537749344690314E-5
78-79	12.754940023644501	7.64548739234198E-5	9.972374859576496E-6	9.307549868938063E-5	4.6537749344690314E-5
80-81	13.662565749113996	7.64548739234198E-5	9.972374859576496E-6	9.307549868938063E-5	4.6537749344690314E-5
82-83	14.58228630322568	7.64548739234198E-5	9.972374859576496E-6	9.307549868938063E-5	4.6537749344690314E-5
84-85	15.513019683307292	7.64548739234198E-5	9.972374859576496E-6	9.307549868938063E-5	4.6537749344690314E-5
86-87	16.45550883128587	7.64548739234198E-5	9.972374859576496E-6	9.473756116597671E-5	4.6537749344690314E-5
88	17.16991314560116	7.64548739234198E-5	9.972374859576496E-6	9.972374859576496E-5	4.6537749344690314E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	168040	0.0	75.27586	1
GTGGTAT	169180	0.0	73.872635	6
AGTGGTA	170535	0.0	73.45924	5
GGTATCA	169485	0.0	73.4569	8
TGGTATC	171095	0.0	72.73821	7
GTATCAA	171320	0.0	72.505554	9
GCAGTGG	179860	0.0	70.288124	3
CAGTGGT	179215	0.0	70.18453	4
AGCAGTG	181240	0.0	70.097084	2
AGTACTT	154855	0.0	40.956776	20-21
CAACGCA	165705	0.0	39.907093	12-13
GAGTACT	148855	0.0	39.90007	20-21
ACGCAGA	167490	0.0	39.521065	14-15
ATCAACG	167160	0.0	39.51476	10-11
AGAGTAC	167575	0.0	39.070824	18-19
GTACTTT	152680	0.0	38.769146	22-23
TACTTTT	166150	0.0	38.220303	22-23
TCAACGC	165425	0.0	37.449505	12-13
AACGCAG	166030	0.0	37.2239	14-15
GCAGAGT	178680	0.0	36.94609	16-17
>>END_MODULE