FastQCFastQC Report
Thu 26 May 2016
SRR1660584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38649917
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17179614.444928044735517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA945650.24467064185415976No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT687360.1778425552634434No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT475320.12298085918269888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2226000.083.0968868
GTGGTAT2256450.081.995866
AGTGGTA2264000.081.830455
GTATCAA2264400.081.5088969
TGGTATC2266450.081.4232257
AAGCAGT2355750.079.800531
GCAGTGG2406400.077.816993
CAGTGGT2408300.077.1389164
AGCAGTG2502950.075.144652
CAACGCA2175550.044.76719312-13
ATCAACG2201650.044.34770210-11
ACGCAGA2231050.043.6795714-15
AGAGTAC2236800.042.97760818-19
TCAACGC2175900.042.49910412-13
AACGCAG2213850.041.6153514-15
AGTACTT2294600.040.92299720-21
GAGTACT2164400.040.86627220-21
CGCAGAG2249850.040.86292616-17
TATCAAC2301250.040.0987310-11
GCAGAGT2439050.039.69478216-17
Produced by FastQC (version 0.11.5)