##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660584_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38649917 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89515866230709 34.0 31.0 34.0 30.0 34.0 2 32.05009772724738 34.0 31.0 34.0 30.0 34.0 3 32.060284605527094 34.0 31.0 34.0 30.0 34.0 4 35.43246550827004 37.0 35.0 37.0 33.0 37.0 5 35.40796079846692 37.0 35.0 37.0 33.0 37.0 6 35.432731796034645 37.0 35.0 37.0 33.0 37.0 7 35.402271808242176 37.0 35.0 37.0 33.0 37.0 8 35.41155288897516 37.0 35.0 37.0 33.0 37.0 9 37.06467553345587 39.0 37.0 39.0 34.0 39.0 10-11 37.0010184109839 39.0 37.0 39.0 34.0 39.0 12-13 37.02575266849861 39.0 37.0 39.0 34.0 39.0 14-15 38.41710673013864 41.0 38.0 41.0 34.0 41.0 16-17 38.387268063214734 40.5 38.0 41.0 34.0 41.0 18-19 38.33364172554368 40.0 38.0 41.0 34.0 41.0 20-21 38.28490934404853 40.0 38.0 41.0 34.0 41.0 22-23 38.14245153488945 40.0 38.0 41.0 33.5 41.0 24-25 38.1324819662614 40.0 38.0 41.0 34.0 41.0 26-27 38.108474851317276 40.0 38.0 41.0 33.5 41.0 28-29 37.99923241749782 40.0 38.0 41.0 33.0 41.0 30-31 37.90766396729907 40.0 38.0 41.0 33.0 41.0 32-33 37.82433935110391 40.0 38.0 41.0 33.0 41.0 34-35 37.71630293539828 40.0 38.0 41.0 33.0 41.0 36-37 37.54285868194749 40.0 38.0 41.0 32.0 41.0 38-39 37.17256769270682 40.0 36.0 41.0 32.0 41.0 40-41 37.05389663579355 40.0 36.0 41.0 31.5 41.0 42-43 36.900754754531555 40.0 36.0 41.0 31.0 41.0 44-45 36.7664241297077 40.0 35.0 41.0 31.0 41.0 46-47 36.60564986465559 40.0 35.0 41.0 31.0 41.0 48-49 36.439585148397605 39.0 35.0 41.0 30.0 41.0 50-51 35.25909256157006 38.0 34.0 40.0 28.5 40.5 52-53 35.276440930002515 38.5 35.0 39.5 28.5 40.5 54-55 35.598149421640414 39.0 35.0 40.5 28.0 41.0 56-57 35.50707092074738 39.0 35.0 41.0 27.0 41.0 58-59 35.35837489638076 39.0 35.0 41.0 26.5 41.0 60-61 35.14513324828097 39.0 35.0 41.0 26.0 41.0 62-63 34.87484314649369 38.5 35.0 41.0 26.0 41.0 64-65 34.582962170397415 38.0 35.0 40.5 26.0 41.0 66-67 34.27449483267972 37.0 35.0 40.0 26.0 41.0 68-69 33.94811909945369 37.0 34.0 40.0 25.0 41.0 70-71 33.61551899322319 36.0 34.0 39.0 25.0 41.0 72-73 33.261475231628566 36.0 34.0 39.0 24.0 41.0 74-75 32.905956964926986 35.0 34.0 38.5 23.5 40.5 76-77 32.5535178122116 35.0 34.0 37.0 22.0 39.5 78-79 32.21010475908655 35.0 33.5 37.0 20.5 39.0 80-81 31.888362800365133 35.0 33.0 36.0 20.0 39.0 82-83 31.500844658993707 35.0 33.0 36.0 18.0 37.5 84-85 31.240107902430942 35.0 33.0 35.5 16.0 37.0 86-87 30.949292323189205 35.0 33.0 35.0 8.5 37.0 88-89 30.760399731259447 35.0 33.0 35.0 6.0 36.0 90-91 30.57057843099637 35.0 33.0 35.0 2.0 36.0 92-93 30.393436187715487 35.0 32.5 35.0 2.0 36.0 94-95 30.173606013177206 35.0 32.0 35.0 2.0 36.0 96-97 30.021899814170364 35.0 32.0 35.0 2.0 35.0 98-99 29.85819069158674 35.0 32.0 35.0 2.0 35.0 100 29.730336186750414 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45733812084210257 1101 2 0.416644815278147 1101 3 0.4740143959386458 1101 4 0.3748199017161511 1101 5 0.3781569903279305 1101 6 0.3975952580936877 1101 7 0.38473757980856504 1101 8 0.4098126762090857 1101 9 0.461831926436858 1101 10-11 0.4996334302737395 1101 12-13 0.48183306389621805 1101 14-15 0.5930971708060326 1101 16-17 0.5877169943944338 1101 18-19 0.5352438447931505 1101 20-21 0.5676438288415042 1101 22-23 0.5750558261944789 1101 24-25 0.5992891867209522 1101 26-27 0.5873785029842367 1101 28-29 0.540846108933529 1101 30-31 0.6000004515496968 1101 32-33 0.5806324213494634 1101 34-35 0.5426449394772419 1101 36-37 0.5516030769358622 1101 38-39 0.599960917947854 1101 40-41 0.5920495105616155 1101 42-43 0.6571607428936588 1101 44-45 0.6359908918579364 1101 46-47 0.610260911042225 1101 48-49 0.6071521878493726 1101 50-51 0.5328097993389775 1101 52-53 0.5763796920104269 1101 54-55 0.6340866113873815 1101 56-57 0.6522882620627186 1101 58-59 0.6373245285310034 1101 60-61 0.6363129224358701 1101 62-63 0.6225548376065575 1101 64-65 0.6451320878515858 1101 66-67 0.6263579960715333 1101 68-69 0.5704118564951344 1101 70-71 0.5399978460619792 1101 72-73 0.5327046011262553 1101 74-75 0.5315746337412719 1101 76-77 0.5838427831577704 1101 78-79 0.5365726702510543 1101 80-81 0.5332324707261762 1101 82-83 0.43834127711926385 1101 84-85 0.41424686401805744 1101 86-87 0.36085240208664615 1101 88-89 0.35748203279367274 1101 90-91 0.2551690976167933 1101 92-93 0.32827406825600747 1101 94-95 0.29871047986801713 1101 96-97 0.24591088852311316 1101 98-99 0.2864652763948605 1101 100 0.27871375753588623 1102 1 0.3385718772578201 1102 2 0.38047195352837093 1102 3 0.3660827148885275 1102 4 0.30409942855758487 1102 5 0.2501542227252358 1102 6 0.2741565162192714 1102 7 0.31597433345897485 1102 8 0.34324438200894036 1102 9 0.40584570877869197 1102 10-11 0.32589465191295375 1102 12-13 0.3948997884786749 1102 14-15 0.4684319239043617 1102 16-17 0.4855099552975375 1102 18-19 0.43286185933210675 1102 20-21 0.43380594246292503 1102 22-23 0.4376381426441043 1102 24-25 0.48683844863682424 1102 26-27 0.4946599940283889 1102 28-29 0.5249957379024579 1102 30-31 0.553113271892471 1102 32-33 0.4962466648530537 1102 34-35 0.45425841897424846 1102 36-37 0.5161752143586682 1102 38-39 0.5193905152246359 1102 40-41 0.42525919072486573 1102 42-43 0.4896606934484353 1102 44-45 0.47133884191151054 1102 46-47 0.420567518044912 1102 48-49 0.4457670160409535 1102 50-51 0.4594998975214395 1102 52-53 0.500175238260752 1102 54-55 0.5112023384305857 1102 56-57 0.5261325878158871 1102 58-59 0.5070278988471415 1102 60-61 0.4781587708100332 1102 62-63 0.4510198940688639 1102 64-65 0.4970050902631655 1102 66-67 0.5135498475854163 1102 68-69 0.4825041483034056 1102 70-71 0.42663133883228 1102 72-73 0.4357185342590455 1102 74-75 0.37960993114121777 1102 76-77 0.40557248387205647 1102 78-79 0.42103443706685084 1102 80-81 0.4300582002896576 1102 82-83 0.32319278109689975 1102 84-85 0.2584198202767034 1102 86-87 0.2446859535305812 1102 88-89 0.1978725088868032 1102 90-91 0.2536562961418838 1102 92-93 0.24052615408107414 1102 94-95 0.27603117937443855 1102 96-97 0.25461862851442163 1102 98-99 0.23762350666770615 1102 100 0.2641254603787928 1103 1 0.3873963449210365 1103 2 0.381452825626738 1103 3 0.3685047869159206 1103 4 0.30427078050676926 1103 5 0.2852501231344604 1103 6 0.3190948181161346 1103 7 0.2816392973562998 1103 8 0.2570451743933262 1103 9 0.37677444698135787 1103 10-11 0.3822094971759711 1103 12-13 0.40543061743643705 1103 14-15 0.469758058320096 1103 16-17 0.459201753527104 1103 18-19 0.4536284772513284 1103 20-21 0.4731929299085067 1103 22-23 0.4600864786756773 1103 24-25 0.43762371346353035 1103 26-27 0.3789679527112426 1103 28-29 0.47806458474716607 1103 30-31 0.515506999690821 1103 32-33 0.43850222694933194 1103 34-35 0.44290505242358336 1103 36-37 0.4605635705117237 1103 38-39 0.45701773008138247 1103 40-41 0.5170819835603524 1103 42-43 0.5086838233329232 1103 44-45 0.45184232493118515 1103 46-47 0.46408714676812224 1103 48-49 0.5131465256399537 1103 50-51 0.6116133771998804 1103 52-53 0.581729784198636 1103 54-55 0.5858623256430562 1103 56-57 0.5777986793925081 1103 58-59 0.5525362019590503 1103 60-61 0.5539814142965511 1103 62-63 0.537004186774638 1103 64-65 0.5416533549062095 1103 66-67 0.5138494603344128 1103 68-69 0.5104888222294477 1103 70-71 0.506358168677373 1103 72-73 0.43660476393835523 1103 74-75 0.464477406353069 1103 76-77 0.47163115414213763 1103 78-79 0.42080327593249933 1103 80-81 0.3850999276202174 1103 82-83 0.3075415922211562 1103 84-85 0.29870086086091163 1103 86-87 0.3208265689905119 1103 88-89 0.2715213696102019 1103 90-91 0.28133063193603647 1103 92-93 0.29237800855861096 1103 94-95 0.2747948425824305 1103 96-97 0.2722044397469965 1103 98-99 0.23812898099083668 1103 100 0.19160850455336842 1104 1 0.27062190272770437 1104 2 0.3176539082639209 1104 3 0.277293564072842 1104 4 0.28351309243858935 1104 5 0.26590647403245526 1104 6 0.30184724660474416 1104 7 0.31082073555036516 1104 8 0.30063621959063624 1104 9 0.3809036341090035 1104 10-11 0.40798941558346513 1104 12-13 0.3964637680414427 1104 14-15 0.4674993173752142 1104 16-17 0.43393456053654944 1104 18-19 0.42629571369696606 1104 20-21 0.38934915384862023 1104 22-23 0.3724787613887912 1104 24-25 0.44444236998172215 1104 26-27 0.41977212343496006 1104 28-29 0.4699472489881842 1104 30-31 0.4691724783510054 1104 32-33 0.43579455355178 1104 34-35 0.43401035704359003 1104 36-37 0.44758608257134824 1104 38-39 0.4308393670748245 1104 40-41 0.440119333342885 1104 42-43 0.3929747916969575 1104 44-45 0.4499893018502732 1104 46-47 0.4305297087100186 1104 48-49 0.4268652751561106 1104 50-51 0.37394574078643217 1104 52-53 0.49491924330385473 1104 54-55 0.5376451234250297 1104 56-57 0.4745772418159149 1104 58-59 0.49936381221299087 1104 60-61 0.5079060511743947 1104 62-63 0.4404497638172131 1104 64-65 0.49727146046280524 1104 66-67 0.46266593333680106 1104 68-69 0.46787727026591597 1104 70-71 0.4126013328380509 1104 72-73 0.4504205722835266 1104 74-75 0.4478609154481532 1104 76-77 0.4955311304366674 1104 78-79 0.46712221236639806 1104 80-81 0.48565719049383915 1104 82-83 0.37310310631850996 1104 84-85 0.34800213275313396 1104 86-87 0.33072528489717357 1104 88-89 0.3308851564148476 1104 90-91 0.3209260089605408 1104 92-93 0.31206728249897964 1104 94-95 0.3438511685763892 1104 96-97 0.31918199498494815 1104 98-99 0.29414435341954004 1104 100 0.3086017738959441 1105 1 0.28314122836655997 1105 2 0.28543703394098685 1105 3 0.2167114308569964 1105 4 0.2744601256324657 1105 5 0.2494967122364713 1105 6 0.23942519304940646 1105 7 0.28217452105983654 1105 8 0.21327414502327713 1105 9 0.35033006708673753 1105 10-11 0.36296237911014373 1105 12-13 0.3383969301928218 1105 14-15 0.41303290221209465 1105 16-17 0.44459476095514816 1105 18-19 0.43697618558510953 1105 20-21 0.36859470881771017 1105 22-23 0.45318732432790654 1105 24-25 0.4364621043280863 1105 26-27 0.45466026011390426 1105 28-29 0.4560913782163496 1105 30-31 0.4002805305278727 1105 32-33 0.4018897540730251 1105 34-35 0.42456124416051466 1105 36-37 0.42375573483288065 1105 38-39 0.4367785609890049 1105 40-41 0.47266543324276 1105 42-43 0.46223734622852675 1105 44-45 0.45615825288327727 1105 46-47 0.39686953983080997 1105 48-49 0.44031161538141106 1105 50-51 0.5360223467108298 1105 52-53 0.5271123714283235 1105 54-55 0.5019094954815344 1105 56-57 0.49712697565692565 1105 58-59 0.46292151709399576 1105 60-61 0.4306381746530903 1105 62-63 0.39150001449550587 1105 64-65 0.396205438610032 1105 66-67 0.41747590465427464 1105 68-69 0.4181829591925066 1105 70-71 0.43112242596899364 1105 72-73 0.3953226249524775 1105 74-75 0.38752201621404225 1105 76-77 0.3607098747133932 1105 78-79 0.33993037926401826 1105 80-81 0.32316516006662255 1105 82-83 0.3125596256738916 1105 84-85 0.2804792624990178 1105 86-87 0.23414975480458367 1105 88-89 0.24050117528922854 1105 90-91 0.2440881661778107 1105 92-93 0.21998113914215622 1105 94-95 0.2603847237808843 1105 96-97 0.1924911319055198 1105 98-99 0.2196926046282357 1105 100 0.1998152088876779 1106 1 0.2403871921768257 1106 2 0.2700356226069758 1106 3 0.28622983120576606 1106 4 0.20904096619600665 1106 5 0.17869566339274456 1106 6 0.2225789211949234 1106 7 0.2525714201625604 1106 8 0.2507257260942666 1106 9 0.3261430350175232 1106 10-11 0.3051224383141573 1106 12-13 0.35069139164335184 1106 14-15 0.35567296756140365 1106 16-17 0.306475339198208 1106 18-19 0.34932547104570233 1106 20-21 0.4208720189910693 1106 22-23 0.42074822761900776 1106 24-25 0.40119777284765235 1106 26-27 0.4007747030247302 1106 28-29 0.4202507148761683 1106 30-31 0.39094845096580144 1106 32-33 0.3461542248784184 1106 34-35 0.40798580806450957 1106 36-37 0.40881470167356326 1106 38-39 0.4108282859991377 1106 40-41 0.42816778210295325 1106 42-43 0.4906770957643616 1106 44-45 0.4662856579888768 1106 46-47 0.44714915378477116 1106 48-49 0.40567907077547005 1106 50-51 0.44142790536700005 1106 52-53 0.47800759399758164 1106 54-55 0.5621377003001342 1106 56-57 0.5424269394026382 1106 58-59 0.5224034931351369 1106 60-61 0.5378095550234008 1106 62-63 0.516973966391788 1106 64-65 0.47345905379037845 1106 66-67 0.422024366132284 1106 68-69 0.4966046484100275 1106 70-71 0.4528081839236151 1106 72-73 0.3255422481072756 1106 74-75 0.39349625689355605 1106 76-77 0.4074285725370572 1106 78-79 0.3989437127218025 1106 80-81 0.3877502331817766 1106 82-83 0.3513198305041456 1106 84-85 0.3169841535850928 1106 86-87 0.3054220677494328 1106 88-89 0.27738046030131613 1106 90-91 0.3290405052760512 1106 92-93 0.3272994395336468 1106 94-95 0.3784422255732771 1106 96-97 0.2592303181009612 1106 98-99 0.21675560972366625 1106 100 0.2105676252357931 1107 1 0.40532635695652885 1107 2 0.40374728898974155 1107 3 0.4419588311777005 1107 4 0.40630686132597305 1107 5 0.3819611002267891 1107 6 0.4241598787951588 1107 7 0.4085293842162372 1107 8 0.45060163933166564 1107 9 0.5530660414205073 1107 10-11 0.5179715563296057 1107 12-13 0.47587423166903875 1107 14-15 0.6270123460668344 1107 16-17 0.5665418990850881 1107 18-19 0.5703827183606762 1107 20-21 0.6144868242613342 1107 22-23 0.6280122392654164 1107 24-25 0.6180299797300748 1107 26-27 0.6703217466626299 1107 28-29 0.6669117337132278 1107 30-31 0.6984782213389238 1107 32-33 0.675637125314374 1107 34-35 0.703533420533887 1107 36-37 0.7077628183087654 1107 38-39 0.6901290037878667 1107 40-41 0.7368475601361766 1107 42-43 0.7138068149246592 1107 44-45 0.7133437721280771 1107 46-47 0.7247569747501075 1107 48-49 0.7397860582139515 1107 50-51 0.7302414445640508 1107 52-53 0.7249371543055716 1107 54-55 0.73889261197575 1107 56-57 0.7315591747911512 1107 58-59 0.6949918975742477 1107 60-61 0.6369206047402045 1107 62-63 0.6816106713510024 1107 64-65 0.6872280242196567 1107 66-67 0.6911843490412934 1107 68-69 0.6780949616959333 1107 70-71 0.633819628905826 1107 72-73 0.6248821812582648 1107 74-75 0.6486977443701463 1107 76-77 0.6413372657594394 1107 78-79 0.6364179472865885 1107 80-81 0.6427832579764754 1107 82-83 0.6004764652488603 1107 84-85 0.5741904250026373 1107 86-87 0.5545008852556421 1107 88-89 0.5418197339733801 1107 90-91 0.5676236051457728 1107 92-93 0.5539444860398532 1107 94-95 0.5838792025276973 1107 96-97 0.5339617315682084 1107 98-99 0.4937499519219841 1107 100 0.5100568354162469 1108 1 0.09384389110589808 1108 2 0.12217263676419066 1108 3 0.0833600819129785 1108 4 0.07884301901091817 1108 5 0.07346112473764066 1108 6 0.15498688385221726 1108 7 0.1641677634934453 1108 8 0.14007774949035934 1108 9 0.07059642392592735 1108 10-11 0.09295188880126659 1108 12-13 0.14967161896565528 1108 14-15 0.16498719813346696 1108 16-17 0.17818954208680537 1108 18-19 0.1754097088324471 1108 20-21 0.12997584481249191 1108 22-23 0.20856371380386207 1108 24-25 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-0.8273046040997798 2316 72-73 -0.8615518457158231 2316 74-75 -0.8309977544642493 2316 76-77 -0.8343920760372647 2316 78-79 -0.8039541396347545 2316 80-81 -0.8116714011633519 2316 82-83 -0.8005617783864238 2316 84-85 -0.77954147951721 2316 86-87 -0.756710167417328 2316 88-89 -0.7265166536347216 2316 90-91 -0.774545311232842 2316 92-93 -0.7727325775748355 2316 94-95 -0.6853135669480217 2316 96-97 -0.7916767368300768 2316 98-99 -0.7908748306707274 2316 100 -0.7072963086248123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 399718.0 3 65897.0 4 43753.0 5 40998.0 6 42674.0 7 45149.0 8 52062.0 9 61999.0 10 72051.0 11 79746.0 12 79658.0 13 78446.0 14 75344.0 15 73737.0 16 86136.0 17 110576.0 18 149595.0 19 232008.0 20 454507.0 21 574711.0 22 243273.0 23 193127.0 24 215482.0 25 245258.0 26 270006.0 27 308286.0 28 366113.0 29 437561.0 30 529573.0 31 656782.0 32 827948.0 33 1073679.0 34 1493714.0 35 2440956.0 36 4252423.0 37 8260212.0 38 1.0549399E7 39 3456035.0 40 11325.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.87297664322292 33.66056637142308 27.677286288282076 22.789170697071924 2 21.571575060257153 32.22583620330389 26.992284397864484 19.210304338574467 3 25.358451284070576 25.836262928176158 26.172879297585872 22.632406490167394 4 20.383278479566705 27.38139501419666 25.63133216681476 26.603994339421877 5 22.108660243929638 31.090956011348318 25.829105863496387 20.97127788122566 6 26.974330622784535 26.118867757532676 26.033623749213834 20.873177870468947 7 21.602284355352225 26.128737853514224 30.905983415238186 21.36299437589536 8 26.500294312538458 26.11212981219657 25.943269353653598 21.444306521611374 9 26.126659449866775 25.53557690449237 26.767595639223362 21.570168006417497 10-11 21.15186605883619 28.872259791048172 28.695750129982162 21.280124020133478 12-13 21.19518889835138 26.339061334342173 28.46309606789062 24.00265369941583 14-15 21.2241409171476 31.506399336906334 25.858283472958277 21.411176272987795 16-17 23.978536549320363 26.14777375720527 25.893208653065663 23.9804810404087 18-19 21.433564319984384 28.78263798316621 25.846141576687742 23.937656120161666 20-21 24.07347539777865 28.75760352485554 25.804160273172368 21.36476080419344 22-23 23.911238245461142 26.229532646030567 28.490882736067675 21.368346372440616 24-25 21.44038677726839 28.515753608842886 25.841064783409568 24.202794830479156 26-27 21.523867366305453 25.956331240231584 31.061195435816185 21.458605957646782 28-29 21.6341242389767 25.997460996592007 30.96545806738736 21.40295669704393 30-31 21.550281973379455 25.973026911631642 30.97256711996281 21.504123995026088 32-33 21.59463490827408 26.010217180087892 30.89259089567644 21.50255701596159 34-35 21.572624225233348 26.08804424981217 30.87681854597059 21.462512978983888 36-37 21.565947618372107 26.054173579197286 30.84164846304494 21.538230339385663 38-39 21.62710087683511 25.99657178223819 30.851248240915613 21.525079100011087 40-41 21.57991476674041 26.071539211060223 30.86267878257292 21.485867239626444 42-43 21.596780973848016 26.065612016691595 30.80354338926704 21.534063620193344 44-45 21.641564736069103 26.050197349361632 30.794257614774207 21.513980299795055 46-47 21.617382937896974 26.121623881515283 30.7687985472622 21.492194633325546 48-49 21.668065402215948 26.091927693365246 30.682971075465055 21.55703582895375 50-51 21.673854349035597 26.119688715223376 30.659439315431975 21.54701762030905 52-53 21.71140700237602 26.256141777301963 30.483407864027505 21.549043356294508 54-55 21.95551786019342 26.47020118736244 29.842980843228023 21.731300109216114 56-57 22.306243333667748 26.876493047553407 28.902459450243 21.914804168535838 58-59 22.335554569368377 27.25603230156307 28.505680339674512 21.90273278939404 60-61 22.292740878667615 27.424597563488234 28.336669258237336 21.945992299606818 62-63 22.25387856282661 27.50948680750134 28.274624250855446 21.96201037881661 64-65 22.22416759062309 27.54490117272289 28.346933349985708 21.883997886668315 66-67 22.202874374091095 27.3388631463471 28.552842104330225 21.90542037523158 68-69 22.186914675981413 27.17581589411487 28.793073259319684 21.84419617058403 70-71 22.142125441601713 27.09970146425907 28.98734134248165 21.770831751657564 72-73 22.13483257432606 27.05756852977639 29.032319334859185 21.775279561038367 74-75 22.20394217528617 27.09388165078524 28.93782880488005 21.764347369048544 76-77 22.27901116379427 27.238827301542585 28.71440496844187 21.76775656622128 78-79 22.402942011192607 27.323422058771406 28.442376078905042 21.831259851130945 80-81 22.46917385827584 27.44550162142925 28.242028605369462 21.84329591492544 82-83 22.46256731864553 27.621120949010958 28.07360389619814 21.84270783614537 84-85 22.49369381990079 27.688147347839987 27.930919329998666 21.887239502260556 86-87 22.547544543772847 27.73155339824071 27.831299140499627 21.889602917486815 88-89 22.576953142188597 27.814178739397267 27.736424487302603 21.872443631111537 90-91 22.651693445190958 27.783189550807375 27.62841651408836 21.936700489913306 92-93 22.68287440999663 27.79795730766381 27.58746187721377 21.93170640512579 94-95 22.726409439965416 27.867915327785862 27.568639407907497 21.837035824341225 96-97 22.723854757827148 27.840824950652415 27.519506577100582 21.91581371441985 98-99 22.798026041127713 27.797655266955783 27.491934931890118 21.91238376002639 100 22.7727500520924 27.87135289598972 27.46198760102214 21.893909450895734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 160892.0 1 93525.0 2 19588.0 3 12002.5 4 10825.0 5 10772.0 6 11262.0 7 11946.5 8 12386.5 9 13029.5 10 14631.5 11 23219.5 12 198076.0 13 226899.0 14 83066.0 15 81185.0 16 89512.0 17 98582.5 18 107147.0 19 116690.5 20 129410.0 21 147828.0 22 172579.5 23 201483.5 24 232707.5 25 268509.5 26 310964.0 27 361256.5 28 421565.5 29 482554.5 30 534564.5 31 588161.0 32 647330.0 33 702326.5 34 755811.0 35 822576.0 36 900511.5 37 956199.5 38 1002135.0 39 1049128.0 40 1080176.5 41 1117804.5 42 1160997.5 43 1187221.5 44 1211195.5 45 1244674.0 46 1275257.0 47 1295075.0 48 1301384.0 49 1298503.5 50 1291574.5 51 1285114.0 52 1277311.0 53 1255580.5 54 1214843.5 55 1163393.5 56 1099705.5 57 1017150.5 58 920674.5 59 810950.0 60 684451.5 61 561047.5 62 449573.0 63 344759.5 64 259614.0 65 193823.0 66 140249.0 67 103010.0 68 78415.5 69 61034.5 70 49572.5 71 41969.0 72 36487.0 73 30293.0 74 24157.5 75 20444.5 76 16744.5 77 11979.5 78 8396.0 79 6108.5 80 4322.0 81 3085.0 82 2214.5 83 1604.5 84 1200.5 85 924.0 86 680.5 87 539.0 88 435.5 89 338.5 90 296.0 91 241.0 92 188.0 93 167.0 94 148.0 95 123.5 96 90.5 97 73.5 98 58.0 99 40.5 100 26.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023428252122766523 2 0.03902725069241417 3 0.05837528706724002 4 0.13569498739156413 5 0.18137943219903938 6 0.1115086482591929 7 0.12475317864201363 8 0.15081274301313508 9 0.1369705399367352 10-11 0.1353508728104125 12-13 0.13577907554109364 14-15 0.148722182249447 16-17 0.13893949629956515 18-19 0.1424220393539267 20-21 0.1516303385593299 22-23 0.16097447246781926 24-25 0.15753203299246413 26-27 0.14859281586555542 28-29 0.1673975134280366 30-31 0.15986709622170728 32-33 0.15603267660316064 34-35 0.14311156218006885 36-37 0.1512719936759502 38-39 0.14666525674557077 40-41 0.14110638322974925 42-43 0.1452344645397298 44-45 0.13846472167068302 46-47 0.1471025151231243 48-49 0.1406057353240888 50-51 0.14338452525008008 52-53 0.15842078004979931 54-55 0.1609447181995242 56-57 0.1552720022658781 58-59 0.15288777981075613 60-61 0.15373901061676276 62-63 0.14742981207437003 64-65 0.14703006994814502 66-67 0.1602422587349929 68-69 0.15432374667195276 70-71 0.14717496029810362 72-73 0.15107276944475717 74-75 0.1509848003037109 76-77 0.11512055769744602 78-79 0.03244120808849344 80-81 0.04125882081454405 82-83 0.17141822063938714 84-85 0.0454748712655709 86-87 0.1871427046014096 88-89 0.04144122741583119 90-91 0.17828628196019153 92-93 0.18782705277219613 94-95 0.24543519718295903 96-97 0.27158919901432127 98-99 0.2993007721077383 100 0.31623612542298607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.8649917E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.005936583460254 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10946610023775 37.7882160569273 2 11.306277718571737 11.081494577426323 3 4.229587421119115 6.218246788006936 4 2.135553143146181 4.186191276145238 5 1.2697788358711202 3.111335055286005 6 0.8384774966287917 2.4654225015869446 7 0.6063479748372111 2.0800255281667344 8 0.39585790990935904 1.5519510103263336 9 0.30498812444898316 1.3451605816909817 >10 1.5959916128180711 14.052182275589153 >50 0.1280568773649781 4.354599418171157 >100 0.07876711450523655 6.073339286838224 >500 3.8297625023654333E-4 0.12114227416761209 >1k 4.035072470003249E-4 0.4426090762727278 >5k 3.685911212366098E-5 0.12062573097851678 >10k+ 2.632793196245826E-5 5.0074585624199015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1717961 4.444928044735517 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94565 0.24467064185415976 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68736 0.1778425552634434 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 47532 0.12298085918269888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0608043479110189E-4 1.0349310711327012E-5 0.0 5.174655355663506E-6 5.174655355663506E-6 2 1.0866776246893363E-4 2.587327677831753E-5 0.0 5.174655355663506E-6 5.174655355663506E-6 3 1.216044008580924E-4 4.139724284530805E-5 0.0 7.76198303349526E-6 1.0349310711327012E-5 4 1.3454103924725116E-4 6.209586426796208E-5 2.587327677831753E-6 1.2936638389158765E-5 1.552396606699052E-5 5 1.5265233299207342E-4 6.985784730145734E-5 2.587327677831753E-6 1.2936638389158765E-5 2.0698621422654024E-5 6 1.604143160255687E-4 6.985784730145734E-5 2.587327677831753E-6 1.552396606699052E-5 2.328594910048578E-5 7 1.759382820925592E-4 7.503250265712084E-5 2.587327677831753E-6 1.552396606699052E-5 2.328594910048578E-5 8 1.811129374482227E-4 8.020715801278435E-5 2.587327677831753E-6 1.552396606699052E-5 2.328594910048578E-5 9 1.9404957583738148E-4 8.27944856906161E-5 2.587327677831753E-6 1.552396606699052E-5 2.587327677831753E-5 10-11 2.0827987806545614E-4 1.0090577943543838E-4 2.587327677831753E-6 1.552396606699052E-5 3.363525981181279E-5 12-13 2.2251018029353077E-4 1.1254875398568126E-4 2.587327677831753E-6 1.8111293744822272E-5 4.39845705231398E-5 14-15 2.4191513787726895E-4 1.319537115694194E-4 2.587327677831753E-6 2.19922852615699E-5 4.9159225878803314E-5 16-17 2.755503976890817E-4 1.4747767763640993E-4 2.587327677831753E-6 2.4579612939401657E-5 5.174655355663506E-5 18-19 3.0918565750089446E-4 1.6170797986448456E-4 2.587327677831753E-6 2.975426829506516E-5 6.209586426796207E-5 20-21 3.3117794276246444E-4 1.759382820925592E-4 2.587327677831753E-6 3.751625132856042E-5 8.020715801278435E-5 22-23 3.583448833796978E-4 1.824066012871386E-4 2.587327677831753E-6 4.1397242845308055E-5 8.667547720736373E-5 24-25 4.2173441148657577E-4 1.9922423119304498E-4 2.587327677831753E-6 5.174655355663506E-5 9.055646872411136E-5 26-27 5.769740721564809E-4 2.1733552493786727E-4 2.587327677831753E-6 5.433388123446682E-5 9.96121155965225E-5 28-29 0.0010530423648775235 2.30272163327026E-4 2.587327677831753E-6 5.56275450733827E-5 1.112550901467654E-4 30-31 0.0022289827944520554 2.4062147403835306E-4 2.587327677831753E-6 5.8214872751214446E-5 1.1384241782459714E-4 32-33 0.004504537487105082 2.4967712091076416E-4 2.587327677831753E-6 5.950853659013032E-5 1.2289806469700828E-4 34-35 0.008363536718591143 2.548517762664277E-4 2.587327677831753E-6 6.209586426796208E-5 1.3066004773050352E-4 36-37 0.014556305515481444 2.703757423334182E-4 2.587327677831753E-6 6.468319194579383E-5 1.3971569460291468E-4 38-39 0.02310354249919864 2.8589970840040875E-4 2.587327677831753E-6 7.373883881820496E-5 1.435966861196623E-4 40-41 0.03436876720847809 3.0013001062848335E-4 2.587327677831753E-6 7.76198303349526E-5 1.5653332450882106E-4 42-43 0.04894447768154327 3.1047932133981034E-4 2.587327677831753E-6 8.020715801278435E-5 1.6817629905906394E-4 44-45 0.06685395986749468 3.272969512457168E-4 2.587327677831753E-6 8.79691410462796E-5 1.720572905758116E-4 46-47 0.08821364351183471 3.4411458115162315E-4 2.587327677831753E-6 8.926280488519548E-5 1.772319459314751E-4 48-49 0.11373504372596713 3.622258748964454E-4 2.587327677831753E-6 9.443746024085899E-5 1.875812566428021E-4 50-51 0.14400936488427646 3.764561771245201E-4 2.587327677831753E-6 9.702478791869074E-5 1.927559119984656E-4 52-53 0.17894605051803864 3.8939281551367885E-4 2.587327677831753E-6 9.831845175760663E-5 1.9793056735412914E-4 54-55 0.2179344912952853 4.100914369363329E-4 2.587327677831753E-6 1.0608043479110189E-4 2.0569255038762436E-4 56-57 0.2616874959912592 4.2173441148657577E-4 2.587327677831753E-6 1.0866776246893363E-4 2.1086720574328788E-4 58-59 0.3098040288158963 4.592506628151362E-4 2.587327677831753E-6 1.1254875398568126E-4 2.1345453342111963E-4 60-61 0.3630538197533516 4.7736195655995845E-4 2.587327677831753E-6 1.1384241782459714E-4 2.1862918877678313E-4 62-63 0.42024669807182247 4.941795864658649E-4 2.587327677831753E-6 1.1384241782459714E-4 2.2897849948811015E-4 64-65 0.4834512322497355 5.084098886939394E-4 2.587327677831753E-6 1.1901707318026064E-4 2.445024655551007E-4 66-67 0.5504733684162892 5.148782078885189E-4 2.587327677831753E-6 1.2289806469700828E-4 2.548517762664277E-4 68-69 0.622231607897114 5.34283165472257E-4 2.587327677831753E-6 1.2548539237484003E-4 2.587327677831753E-4 70-71 0.6995603638683106 5.459261400224999E-4 2.587327677831753E-6 1.3066004773050352E-4 2.639074231388388E-4 72-73 0.7818257410488101 5.66624761445154E-4 2.587327677831753E-6 1.3454103924725116E-4 2.742567338501658E-4 74-75 0.8695827212255074 5.834423913510603E-4 2.587327677831753E-6 1.384220307639988E-4 2.8201871688366114E-4 76-77 0.9629542024630997 5.963790297402191E-4 2.587327677831753E-6 1.4489034995857818E-4 2.8978069991715636E-4 78-79 1.0613502740510414 6.157839873239572E-4 2.587327677831753E-6 1.5265233299207342E-4 3.0142367446739924E-4 80-81 1.1658071607243037 6.300142895520319E-4 2.587327677831753E-6 1.5653332450882106E-4 3.169476405343898E-4 82-83 1.2772265461786114 6.4941924713577E-4 2.587327677831753E-6 1.5912065218665284E-4 3.221222958900533E-4 84-85 1.3942190354509687 6.727051962362558E-4 2.587327677831753E-6 1.604143160255687E-4 3.363525981181279E-4 86-87 1.5180769987164524 6.972848091756575E-4 2.587327677831753E-6 1.6170797986448456E-4 3.4928923650728665E-4 88 1.6146399486446503 7.063404560480686E-4 2.587327677831753E-6 1.6300164370340044E-4 3.544638918629502E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 222600 0.0 83.096886 8 GTGGTAT 225645 0.0 81.99586 6 AGTGGTA 226400 0.0 81.83045 5 GTATCAA 226440 0.0 81.508896 9 TGGTATC 226645 0.0 81.423225 7 AAGCAGT 235575 0.0 79.80053 1 GCAGTGG 240640 0.0 77.81699 3 CAGTGGT 240830 0.0 77.138916 4 AGCAGTG 250295 0.0 75.14465 2 CAACGCA 217555 0.0 44.767193 12-13 ATCAACG 220165 0.0 44.347702 10-11 ACGCAGA 223105 0.0 43.67957 14-15 AGAGTAC 223680 0.0 42.977608 18-19 TCAACGC 217590 0.0 42.499104 12-13 AACGCAG 221385 0.0 41.61535 14-15 AGTACTT 229460 0.0 40.922997 20-21 GAGTACT 216440 0.0 40.866272 20-21 CGCAGAG 224985 0.0 40.862926 16-17 TATCAAC 230125 0.0 40.09873 10-11 GCAGAGT 243905 0.0 39.694782 16-17 >>END_MODULE