FastQCFastQC Report
Thu 26 May 2016
SRR1660584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38649917
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16586594.291494338784737No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT847560.21929154466230807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA772090.19976498267771184No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT495640.128238309024053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2157450.082.650838
GTGGTAT2195650.081.43336
AGTGGTA2202850.081.3697745
TGGTATC2198350.081.074667
GTATCAA2197100.080.988189
AAGCAGT2281900.079.079861
GCAGTGG2348050.076.87633
CAGTGGT2359350.076.261144
AGCAGTG2434100.074.466752
CAACGCA2104450.044.61461312-13
ATCAACG2127300.044.11219810-11
ACGCAGA2163300.043.5247314-15
AGAGTAC2190100.042.7062118-19
TCAACGC2099050.042.2625212-13
AACGCAG2135900.041.4783814-15
AGTACTT2247400.040.64841520-21
GAGTACT2110600.040.61858720-21
CGCAGAG2189150.040.4608516-17
TATCAAC2238550.039.74743310-11
GCAGAGT2387700.039.4174816-17
Produced by FastQC (version 0.11.5)