##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38649917
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51879622406434	34.0	31.0	34.0	31.0	34.0
2	32.801074424040806	34.0	31.0	34.0	31.0	34.0
3	32.884385780181624	34.0	31.0	34.0	31.0	34.0
4	36.29995414996622	37.0	37.0	37.0	35.0	37.0
5	36.223114735278735	37.0	37.0	37.0	35.0	37.0
6	36.21002233976337	37.0	37.0	37.0	35.0	37.0
7	36.1482708746826	37.0	36.0	37.0	35.0	37.0
8	36.176625295210854	37.0	37.0	37.0	35.0	37.0
9	37.94164440249639	39.0	38.0	39.0	35.0	39.0
10-11	37.86227627603961	39.0	38.0	39.0	35.0	39.0
12-13	37.906207263006536	39.0	38.0	39.0	35.0	39.0
14-15	39.41725365154083	41.0	39.0	41.0	36.0	41.0
16-17	39.39969783117516	41.0	39.0	41.0	36.0	41.0
18-19	39.37954452787052	41.0	39.0	41.0	36.0	41.0
20-21	39.330464590648404	41.0	39.0	41.0	36.0	41.0
22-23	39.202536308163346	41.0	39.0	41.0	35.5	41.0
24-25	39.226881780884554	41.0	39.0	41.0	36.0	41.0
26-27	39.20497231598195	41.0	39.0	41.0	36.0	41.0
28-29	39.14262593371158	41.0	39.0	41.0	36.0	41.0
30-31	39.05564876116034	41.0	39.0	41.0	35.0	41.0
32-33	39.00041384306206	40.0	39.0	41.0	35.0	41.0
34-35	38.873550802191886	40.0	38.0	41.0	35.0	41.0
36-37	38.75280595039828	40.0	38.0	41.0	35.0	41.0
38-39	38.45372255262541	40.0	38.0	41.0	34.5	41.0
40-41	38.34247689328802	40.0	38.0	41.0	34.0	41.0
42-43	38.24641694573367	40.0	37.5	41.0	34.0	41.0
44-45	38.152949319399575	40.0	37.0	41.0	34.0	41.0
46-47	38.022616736796614	40.0	37.0	41.0	33.0	41.0
48-49	37.875443574691246	40.0	37.0	41.0	33.0	41.0
50-51	37.70010445300568	40.0	36.0	41.0	33.0	41.0
52-53	37.3697768225479	40.0	36.0	41.0	33.0	41.0
54-55	36.79923360508122	39.5	36.0	41.0	31.5	41.0
56-57	36.28135717600635	39.0	35.0	41.0	30.5	41.0
58-59	36.203916556923005	39.0	35.0	41.0	30.5	41.0
60-61	35.99918518583106	39.0	35.0	41.0	30.0	41.0
62-63	35.696767382450005	38.5	35.0	40.5	29.5	41.0
64-65	35.583020152410676	38.0	35.0	40.0	30.0	41.0
66-67	35.34654872609429	37.0	35.0	40.0	30.0	41.0
68-69	35.04104261077714	37.0	35.0	40.0	30.0	41.0
70-71	34.70322179475832	36.0	35.0	39.0	29.0	41.0
72-73	34.35722886545914	36.0	35.0	39.0	29.0	41.0
74-75	33.99870804379735	35.0	34.0	38.5	29.0	40.0
76-77	32.445699210686534	34.5	32.0	36.5	26.5	39.0
78-79	33.31905391931372	35.0	34.0	37.0	28.5	39.0
80-81	33.22811104613756	35.0	34.0	36.5	29.0	39.0
82-83	32.98192341525598	35.0	34.0	36.0	29.0	37.5
84-85	32.748808166910166	35.0	34.0	36.0	29.0	37.0
86-87	32.53914672054793	35.0	34.0	35.0	28.5	37.0
88-89	32.35619957476235	35.0	34.0	35.0	28.0	36.0
90-91	32.184727822313306	35.0	34.0	35.0	27.0	36.0
92-93	32.02160416023662	35.0	34.0	35.0	27.0	36.0
94-95	31.897643079026533	35.0	34.0	35.0	27.0	36.0
96-97	31.73614833377262	35.0	34.0	35.0	26.0	35.5
98-99	31.598814623586385	35.0	34.0	35.0	25.0	35.0
100	31.50863467572259	35.0	34.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5350039536954725
1101	2	0.3884621800837422
1101	3	0.3317865304155134
1101	4	0.22821736819027194
1101	5	0.237659326593608
1101	6	0.21503089169410572
1101	7	0.2600858354121982
1101	8	0.23299838876352652
1101	9	0.3378008660397782
1101	10-11	0.3088092580098376
1101	12-13	0.3246162563485697
1101	14-15	0.43036788765424205
1101	16-17	0.42563099607494337
1101	18-19	0.38073245078550855
1101	20-21	0.3396020885916258
1101	22-23	0.20201925067714654
1101	24-25	0.33767800799969194
1101	26-27	0.39589052625340315
1101	28-29	0.41243185622659695
1101	30-31	0.3793196847381708
1101	32-33	0.3908451142333007
1101	34-35	0.4307054895680622
1101	36-37	0.4095309483267471
1101	38-39	0.3313097854958329
1101	40-41	0.0019698437670498947
1101	42-43	0.10350564632486936
1101	44-45	0.2765809030235076
1101	46-47	0.31649024594058517
1101	48-49	0.3283122156850311
1101	50-51	0.35309469042392294
1101	52-53	0.2655957819069812
1101	54-55	0.14004779770649378
1101	56-57	0.14582281678296027
1101	58-59	0.1147381104024916
1101	60-61	0.15958991230913
1101	62-63	0.13618159521841022
1101	64-65	0.17459612356748266
1101	66-67	0.13475392823472276
1101	68-69	0.16587815059733657
1101	70-71	0.20283581323224809
1101	72-73	0.14876345786157685
1101	74-75	0.0761212488392502
1101	76-77	0.022409116086343772
1101	78-79	0.1656433902116916
1101	80-81	0.19283127942129852
1101	82-83	0.18592440163342872
1101	84-85	0.19030985529570188
1101	86-87	0.1679978883981761
1101	88-89	0.14673063866158742
1101	90-91	0.13450225980546548
1101	92-93	0.0753207274137182
1101	94-95	0.10945425221312632
1101	96-97	0.09650314413347161
1101	98-99	0.14603433333327587
1101	100	0.15864549958657292
1102	1	0.45209622169679164
1102	2	0.30022458785875017
1102	3	0.24787310544796526
1102	4	0.17488244505872785
1102	5	0.20727655557762859
1102	6	0.20367267956745394
1102	7	0.21118545631728836
1102	8	0.21767401241182682
1102	9	0.2776623473362392
1102	10-11	0.22738440092177115
1102	12-13	0.2794602018551373
1102	14-15	0.3175358118210454
1102	16-17	0.29447515487419906
1102	18-19	0.29112957797234174
1102	20-21	0.24974072802559988
1102	22-23	0.30184996643456685
1102	24-25	0.34937438402891274
1102	26-27	0.34114909105670677
1102	28-29	0.2836363356484384
1102	30-31	0.3427524643266864
1102	32-33	0.332829140790146
1102	34-35	0.4037040939039258
1102	36-37	0.4183976811392185
1102	38-39	0.3819636443842853
1102	40-41	0.3879041199720419
1102	42-43	0.38570989956706825
1102	44-45	0.3703770769025141
1102	46-47	0.33887302011726206
1102	48-49	0.34689233991420565
1102	50-51	0.30476427765919567
1102	52-53	0.37256770605203116
1102	54-55	0.39830395162483256
1102	56-57	0.26719519713438444
1102	58-59	0.18574880798685456
1102	60-61	0.16831644097376852
1102	62-63	0.1825581936740619
1102	64-65	0.22470360858154947
1102	66-67	0.17173047313353607
1102	68-69	0.16961794356851811
1102	70-71	0.14356808734957127
1102	72-73	0.09721298306609327
1102	74-75	0.148863147043933
1102	76-77	-0.1334450114585195
1102	78-79	0.18944375584448636
1102	80-81	0.19160136443763065
1102	82-83	0.030218552931799536
1102	84-85	0.1318784241914912
1102	86-87	0.1900647676715792
1102	88-89	0.21167575799893967
1102	90-91	0.2188761340196308
1102	92-93	0.1822921776239923
1102	94-95	0.17045761793336567
1102	96-97	0.24155027959983855
1102	98-99	0.2620559698068732
1102	100	0.24976078641813615
1103	1	0.425797997801034
1103	2	0.29162840724397654
1103	3	0.2562487496149046
1103	4	0.19029966385828345
1103	5	0.1796247546595211
1103	6	0.19005172426424366
1103	7	0.2070223024114597
1103	8	0.18596650225275368
1103	9	0.2311669707716888
1103	10-11	0.2234865909180357
1103	12-13	0.2372325227497427
1103	14-15	0.26249106100038233
1103	16-17	0.29070726269422664
1103	18-19	0.28245078206578
1103	20-21	0.2703269106134414
1103	22-23	0.3130109452367975
1103	24-25	0.31834470507580903
1103	26-27	0.2641451470359968
1103	28-29	0.28315454649511196
1103	30-31	0.31002514626980116
1103	32-33	0.28298149433649655
1103	34-35	0.3433767710370006
1103	36-37	0.31303346771385065
1103	38-39	0.287943113603383
1103	40-41	0.2657739331882425
1103	42-43	0.28387197156172306
1103	44-45	0.2740706890962272
1103	46-47	0.27594587215085653
1103	48-49	0.30360561096208016
1103	50-51	0.3180693605897815
1103	52-53	0.28522875627398747
1103	54-55	0.26966125915582495
1103	56-57	0.25414846092537147
1103	58-59	0.21247272584196963
1103	60-61	0.18525551466293422
1103	62-63	0.18609000846240775
1103	64-65	0.17826810576153207
1103	66-67	0.16788272060112774
1103	68-69	0.19775062493629747
1103	70-71	0.13906519562230812
1103	72-73	0.17544701501859095
1103	74-75	0.17504565252404092
1103	76-77	0.2191454246777127
1103	78-79	0.23066962206416974
1103	80-81	0.22878763608590447
1103	82-83	0.23441555657645097
1103	84-85	0.21305666084334263
1103	86-87	0.24172247424415616
1103	88-89	0.21760325627181487
1103	90-91	0.19950720991352
1103	92-93	0.20654709812513516
1103	94-95	0.19259073571278407
1103	96-97	0.21924237401320923
1103	98-99	0.257896080006784
1103	100	0.26790687719842055
1104	1	0.39163326142344346
1104	2	0.2735674815179294
1104	3	0.19940567292255906
1104	4	0.15802049187914946
1104	5	0.13724034418834918
1104	6	0.16589147147115568
1104	7	0.17370223442939903
1104	8	0.16876839450135606
1104	9	0.2277614337434244
1104	10-11	0.16831517739219493
1104	12-13	0.19673677868090778
1104	14-15	0.22007175221369835
1104	16-17	0.26320153077126207
1104	18-19	0.27429329859474905
1104	20-21	0.24845407454437662
1104	22-23	0.2689507922092602
1104	24-25	0.2837878500871298
1104	26-27	0.24746190553197778
1104	28-29	0.23728501692438897
1104	30-31	0.3063273793238679
1104	32-33	0.27219581415754845
1104	34-35	0.35255646398717744
1104	36-37	0.2592338590395826
1104	38-39	0.33108674183672804
1104	40-41	0.29150907789883007
1104	42-43	0.3198667555013017
1104	44-45	0.19525629967849767
1104	46-47	0.2509779530509064
1104	48-49	0.29998098275138574
1104	50-51	0.31328103315394884
1104	52-53	0.28558174277433324
1104	54-55	0.31351486064696843
1104	56-57	0.21225786237145172
1104	58-59	0.22828148018557215
1104	60-61	0.26193832676562323
1104	62-63	0.22164863737963714
1104	64-65	0.1938396787896579
1104	66-67	0.22917517406303034
1104	68-69	0.19496350148403963
1104	70-71	0.18883913014236242
1104	72-73	0.2125186482512973
1104	74-75	0.2464977951453946
1104	76-77	-0.05957500521277126
1104	78-79	0.22255251135101872
1104	80-81	0.25908669894297276
1104	82-83	0.2463096569283323
1104	84-85	0.23075214495992924
1104	86-87	0.12314862546231353
1104	88-89	0.1966775971648076
1104	90-91	0.21774001274821586
1104	92-93	0.22156161016466314
1104	94-95	0.2712073940008253
1104	96-97	0.2980162899235559
1104	98-99	0.33356614188924993
1104	100	0.35038905035547785
1105	1	0.4079046069009422
1105	2	0.2787040792027682
1105	3	0.21974175571184418
1105	4	0.1579612381789346
1105	5	0.14543870063787523
1105	6	0.1268683231019807
1105	7	0.13701020416331744
1105	8	0.1370671023937291
1105	9	0.2096970586579019
1105	10-11	0.19491101982913506
1105	12-13	0.20173180389058132
1105	14-15	0.24242565207656241
1105	16-17	0.24714984825481423
1105	18-19	0.2176419489707797
1105	20-21	0.21408283576197107
1105	22-23	0.2530281037379467
1105	24-25	0.1776553689249667
1105	26-27	0.22168279661493528
1105	28-29	0.20736944019799353
1105	30-31	0.24955708398543663
1105	32-33	0.22561100259905942
1105	34-35	0.20010167997838835
1105	36-37	0.18693776304056087
1105	38-39	0.1621092429498674
1105	40-41	0.18257847425223162
1105	42-43	0.27100601645019395
1105	44-45	0.28277920075503715
1105	46-47	0.31034689290208917
1105	48-49	0.32261818906815165
1105	50-51	0.2771610684813126
1105	52-53	0.2663537296797003
1105	54-55	0.28296934461391743
1105	56-57	0.25839674890553965
1105	58-59	0.20527880201293414
1105	60-61	0.20075330294931604
1105	62-63	0.23457643658515792
1105	64-65	0.1895664459156876
1105	66-67	0.24526111011874718
1105	68-69	0.2540379186987849
1105	70-71	0.20878141475439094
1105	72-73	0.22524389796076605
1105	74-75	0.20846098786884681
1105	76-77	0.08671029114246664
1105	78-79	0.2956645429610134
1105	80-81	0.25534620831553667
1105	82-83	0.2723397394328515
1105	84-85	0.25602843709583567
1105	86-87	0.23172650199108347
1105	88-89	0.22680547014604002
1105	90-91	0.24216429669368722
1105	92-93	0.2560117147979142
1105	94-95	0.297294276607893
1105	96-97	0.2565065572344345
1105	98-99	0.28482451079531756
1105	100	0.31229827427806
1106	1	0.389353588232602
1106	2	0.22263479672805886
1106	3	0.18336123718417952
1106	4	0.1438573915896697
1106	5	0.17098955075346822
1106	6	0.14551376680224593
1106	7	0.1303909693305627
1106	8	0.1435613500958013
1106	9	0.18147646274802298
1106	10-11	0.16337933963607298
1106	12-13	0.22325995746853522
1106	14-15	0.2128172369366439
1106	16-17	0.19952371200542274
1106	18-19	0.1789354759428079
1106	20-21	0.22089819693142232
1106	22-23	0.27152017386035965
1106	24-25	0.22614584028306695
1106	26-27	0.22271789736831238
1106	28-29	0.21080551599384023
1106	30-31	0.29182194364747005
1106	32-33	0.245429684496429
1106	34-35	0.19608257556641462
1106	36-37	0.2157195028778176
1106	38-39	0.15665898155507563
1106	40-41	0.2476576196589093
1106	42-43	0.2642365400576594
1106	44-45	0.22761390918562796
1106	46-47	0.2678885218481497
1106	48-49	0.28539660482526585
1106	50-51	0.3027592110839521
1106	52-53	0.2965272675821211
1106	54-55	0.24995148843000692
1106	56-57	0.26015131332069785
1106	58-59	0.24474708941514933
1106	60-61	0.25907163403655886
1106	62-63	0.2440130317767384
1106	64-65	0.24539938426856622
1106	66-67	0.2430926789285266
1106	68-69	0.2285411618985762
1106	70-71	0.245344184088502
1106	72-73	0.2570789259908466
1106	74-75	0.24873817946590293
1106	76-77	-0.5686747193279373
1106	78-79	0.19013269353599327
1106	80-81	0.2259823107800898
1106	82-83	0.2129837737140292
1106	84-85	0.2573388059774473
1106	86-87	0.2384873009736026
1106	88-89	0.22295502802975875
1106	90-91	0.25409725380362147
1106	92-93	0.2331834034403002
1106	94-95	0.21918197797370453
1106	96-97	0.2786026150348171
1106	98-99	0.29700103023872515
1106	100	0.3190535410145898
1107	1	0.5142880930347147
1107	2	0.3754883222942169
1107	3	0.2989578209517205
1107	4	0.19710515672770157
1107	5	0.24444678592453073
1107	6	0.21668573064089003
1107	7	0.18206433512833087
1107	8	0.23643751641735378
1107	9	0.261176442032486
1107	10-11	0.3076639840223905
1107	12-13	0.32559031683823747
1107	14-15	0.3318607109298597
1107	16-17	0.35401346738358086
1107	18-19	0.3557234773142497
1107	20-21	0.368174554671576
1107	22-23	0.42628946256927946
1107	24-25	0.4303378236820521
1107	26-27	0.45786903124746203
1107	28-29	0.43462222699912445
1107	30-31	0.44978126694326903
1107	32-33	0.44501813231182297
1107	34-35	0.44157496320446654
1107	36-37	0.3988147821407466
1107	38-39	0.47015664752785824
1107	40-41	0.5224194271771339
1107	42-43	0.4353462859130346
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2316	96-97	-0.4968006915837755
2316	98-99	-0.5116108423103114
2316	100	-0.530972678616088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	227.0
3	120.0
4	219.0
5	537.0
6	1028.0
7	2239.0
8	4794.0
9	8083.0
10	12328.0
11	17378.0
12	24113.0
13	31506.0
14	35114.0
15	35293.0
16	44527.0
17	64158.0
18	99694.0
19	179756.0
20	422494.0
21	547089.0
22	204107.0
23	137097.0
24	148279.0
25	167419.0
26	197297.0
27	243446.0
28	309891.0
29	392825.0
30	487555.0
31	608221.0
32	768123.0
33	998089.0
34	1393226.0
35	2307366.0
36	4134761.0
37	8826799.0
38	1.1946678E7
39	3835953.0
40	12088.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.26520294222836	33.20897414174417	27.987042364727028	22.538780551300444
2	21.737064387434074	32.101895272626926	27.15530146028303	19.00573887965597
3	25.094659323589237	25.99694845399021	26.466763175713936	22.44162904670662
4	20.641211208810617	27.5660307368836	25.86527417381	25.92748388049578
5	22.175025628127482	30.88728754579214	25.97356418643797	20.964122639642408
6	26.711374309031505	26.221735482640234	26.183494779561883	20.88339542876638
7	21.592020495153974	26.23054792071093	30.80780483952915	21.369626744605945
8	26.20862808062434	26.175261902891023	26.13186206842307	21.48424794806157
9	25.837258589714434	25.56955296954454	26.986898833443806	21.606289607297217
10-11	21.15011915808254	28.78304628700755	28.7537046974771	21.313129857432813
12-13	21.220648416916394	26.401992014627098	28.466221544537856	23.911138023918653
14-15	21.22112836620063	31.308966329733646	26.00585532952115	21.464049974544576
16-17	23.870731727573954	26.194912656604153	26.03411412241843	23.900241493403467
18-19	21.418980020060584	28.7106722635394	25.99837019054918	23.87197752585083
20-21	23.94375646395308	28.667607488005732	25.968160552582813	21.420475495458373
22-23	23.794780723591206	26.260504000564865	28.530986237305505	21.413729038538428
24-25	21.46507634581315	28.43722795461407	25.9835706968947	24.114125002678083
26-27	21.54269179131108	25.994932129732263	30.962214168814512	21.50016191014215
28-29	21.614633307827923	26.026734574812448	30.891214308581954	21.467417808777682
30-31	21.62506270700299	25.997137431622097	30.848809601545668	21.528990259829246
32-33	21.63036749937612	26.03336305180351	30.781978662051273	21.554290786769094
34-35	21.64984352379461	26.101394158616785	30.744772076649753	21.503990240938855
36-37	21.61506300768198	26.063766521393855	30.740502198057317	21.580668272866845
38-39	21.639289678164122	26.018280970693937	30.756698649572776	21.585730701569165
40-41	21.62174630284458	26.08571966661662	30.766794402171676	21.52573962836712
42-43	21.62650957309947	26.070706697766	30.7217115110493	21.58107221808523
44-45	21.657882214857022	26.067763612532467	30.70693916367272	21.567415008937793
46-47	21.64291969889612	26.13093321778673	30.68907495972113	21.537072123596023
48-49	21.68759508590924	26.11308324413737	30.60916793171897	21.59015373823442
50-51	21.703670152771608	26.12324626725589	30.5635830990271	21.609500480945407
52-53	21.749339338633806	26.266219750154963	30.378676977810493	21.60576393340074
54-55	21.987380591034544	26.447426408618963	29.7610814528723	21.804111547474193
56-57	22.324764059487258	26.80943573865178	28.885810873573803	21.979989328287157
58-59	22.351406971086465	27.17472650066751	28.493921420190837	21.979945108055183
60-61	22.29851102900965	27.328845610950815	28.33564877406765	22.036994585971886
62-63	22.255904756415106	27.415064274826978	28.277124248238422	22.051906720519494
64-65	22.19695797447823	27.487244103435966	28.326928136947565	21.98886978513824
66-67	22.176038556159046	27.303720878197176	28.50275348650248	22.0174870791413
68-69	22.12387157260907	27.159674107450215	28.725661946440923	21.990792373499794
70-71	22.087802931116254	27.091253520673796	28.917221219388388	21.90372232882156
72-73	22.068289180313354	27.039236636289647	28.958313472882086	21.934160710514913
74-75	22.110986505374683	27.0642249427463	28.88082573861887	21.94396281326014
76-77	22.12640197907799	27.18611659631766	28.739782287242686	21.947699137361667
78-79	22.200061943504423	27.283944214416273	28.48596540811943	22.030028433959874
80-81	22.24340541067536	27.38785887421315	28.300566249115068	22.068169465996426
82-83	22.224232396886872	27.561438966802605	28.144703534086794	22.06962510222372
84-85	22.22842058213084	27.630244644769775	28.002742949001018	22.138591824098363
86-87	22.25884129694978	27.67230846532309	27.90195509718479	22.16689514054234
88-89	22.25225860075821	27.75925788525267	27.817666157509493	22.170817356479628
90-91	22.282081262977805	27.72483850741575	27.715674697025484	22.277405532580964
92-93	22.29880213982417	27.74351797781237	27.691916219091127	22.265763663272335
94-95	22.28061475889605	27.81607173717956	27.668788107213633	22.23452539671075
96-97	22.301201836992302	27.77714632608396	27.622732022943286	22.298919813980454
98-99	22.289619089923267	27.761519659945037	27.606027071014584	22.342834179117112
100	22.291102704127553	27.81814472997819	27.56404043500474	22.32671213088952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158993.0
1	92927.0
2	19461.0
3	10924.5
4	9529.5
5	9047.0
6	8830.0
7	8840.5
8	8513.0
9	8400.5
10	9247.0
11	17219.5
12	194353.0
13	221025.0
14	71925.5
15	69470.5
16	77914.0
17	87513.5
18	97194.5
19	107561.5
20	121383.5
21	141570.5
22	167498.5
23	197674.5
24	229753.5
25	265958.0
26	309047.5
27	359747.5
28	420802.5
29	482946.0
30	536693.0
31	590435.5
32	650355.5
33	707156.5
34	760802.5
35	826826.5
36	905610.0
37	962603.5
38	1007682.0
39	1054784.5
40	1087416.0
41	1124774.0
42	1168403.0
43	1194968.5
44	1217226.0
45	1252818.5
46	1283565.0
47	1300905.5
48	1308317.5
49	1305188.0
50	1299757.5
51	1295929.0
52	1287664.5
53	1266540.0
54	1225176.5
55	1171465.0
56	1107886.0
57	1025002.5
58	926766.0
59	815358.0
60	686341.0
61	559213.0
62	445278.5
63	339831.5
64	254394.0
65	189011.5
66	135809.0
67	98573.5
68	74316.0
69	57807.0
70	46747.0
71	39598.0
72	34603.5
73	28417.5
74	22111.5
75	18672.0
76	15129.5
77	10473.5
78	7089.5
79	4646.5
80	2892.5
81	1966.5
82	1261.5
83	717.0
84	439.0
85	277.0
86	153.5
87	86.0
88	54.0
89	39.0
90	36.0
91	29.5
92	22.5
93	14.0
94	7.5
95	3.0
96	1.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02786034443489232
2	0.011371305144070556
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007433392418410627
26-27	0.02898065731939347
28-29	0.03368830002920834
30-31	0.030758151434063883
32-33	0.03770383258520322
34-35	0.030463196078791062
36-37	2.742567338501658E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.005901694433134229
54-55	0.03364690278636304
56-57	0.04626271254347066
58-59	0.051335168455859816
60-61	0.05578795938940826
62-63	0.0375059020178491
64-65	0.0011345431867292237
66-67	2.3285949100485776E-5
68-69	0.0
70-71	0.0
72-73	8.150082185170022E-5
74-75	2.8460604456149286E-5
76-77	0.0
78-79	4.721873012042949E-4
80-81	0.017049195733072335
82-83	0.04115274037975295
84-85	0.05541797153147832
86-87	0.05941151180221164
88-89	0.058688353716257656
90-91	0.036848720787679826
92-93	0.002692114448783939
94-95	1.2936638389158767E-6
96-97	0.0
98-99	4.2690906684223926E-4
100	1.0090577943543838E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.8649917E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.8702444095973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10533898414972	36.431811780465004
2	11.763627742696697	11.262554703728203
3	4.419752538465847	6.3472390273889445
4	2.223301706865138	4.257199844156361
5	1.2956839180214	3.101235291663452
6	0.8788563785616574	2.5242641785608066
7	0.6259448072239607	2.097489163611048
8	0.4349739585774631	1.6657847767130567
9	0.33775558888829477	1.4551598331711122
>10	1.6953186558389948	14.57578494859095
>50	0.13583532132914272	4.5127563518503875
>100	0.08277385528026826	6.283709187434002
>500	3.621325464343023E-4	0.10872451192621851
>1k	4.204445374567198E-4	0.41864977169963424
>5k	2.6983508999267216E-5	0.09427912273188545
>10k+	2.6983508568241098E-5	4.8633575063092795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1658659	4.291494338784737	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84756	0.21929154466230807	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77209	0.19976498267771184	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	49564	0.128238309024053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1384241782459714E-4	1.2936638389158765E-5	0.0	0.0	0.0
2	1.267790562137559E-4	1.552396606699052E-5	0.0	2.587327677831753E-6	0.0
3	1.4489034995857818E-4	1.8111293744822272E-5	0.0	7.76198303349526E-6	5.174655355663506E-6
4	1.655889713812322E-4	3.104793213398104E-5	0.0	7.76198303349526E-6	1.0349310711327012E-5
5	1.7335095441472747E-4	5.174655355663506E-5	0.0	1.0349310711327012E-5	1.2936638389158765E-5
6	1.7852560977039096E-4	5.433388123446682E-5	0.0	1.2936638389158765E-5	2.587327677831753E-5
7	1.9663690351521325E-4	6.209586426796208E-5	0.0	1.2936638389158765E-5	2.8460604456149286E-5
8	1.99224231193045E-4	7.244517497928909E-5	0.0	2.8460604456149286E-5	2.8460604456149286E-5
9	2.1474819726003552E-4	8.020715801278435E-5	0.0	2.8460604456149286E-5	3.363525981181279E-5
10-11	2.276848356491943E-4	9.443746024085899E-5	0.0	2.8460604456149286E-5	4.1397242845308055E-5
12-13	2.4838345707184833E-4	1.0866776246893363E-4	0.0	3.104793213398104E-5	4.527823436205568E-5
14-15	2.729630700112499E-4	1.15136081663513E-4	0.0	3.363525981181279E-5	4.786556203988744E-5
16-17	2.9624901911173574E-4	1.3066004773050352E-4	0.0	3.363525981181279E-5	5.562754507338269E-5
18-19	3.2988427892354855E-4	1.6429530754231633E-4	0.0	3.751625132856042E-5	6.209586426796208E-5
20-21	3.5575755570186604E-4	1.8887492048171798E-4	0.0	4.39845705231398E-5	6.59768557847097E-5
22-23	3.8421816015801535E-4	2.0181155887087675E-4	0.0	5.174655355663506E-5	7.373883881820496E-5
24-25	4.5666333513730447E-4	2.1862918877678313E-4	0.0	6.338952810687796E-5	8.279448569061611E-5
26-27	6.274269618742001E-4	2.250975079713625E-4	0.0	6.727051962362559E-5	8.926280488519548E-5
28-29	0.0011267812036957286	2.4191513787726895E-4	0.0	7.632616649603673E-5	9.443746024085899E-5
30-31	0.0023156582716594193	2.4579612939401655E-4	0.0	8.020715801278435E-5	9.96121155965225E-5
32-33	0.004610617921896184	2.664947508166706E-4	0.0	8.667547720736373E-5	1.0608043479110189E-4
34-35	0.008451505859637422	2.8848703607824047E-4	0.0	8.926280488519548E-5	1.1513608166351302E-4
36-37	0.014587353447615425	3.0918565750089446E-4	0.0	9.573112407977488E-5	1.2419172853592417E-4
38-39	0.02316693202730552	3.195349682122215E-4	0.0	1.0219944327435425E-4	1.319537115694194E-4
40-41	0.0344748476432692	3.2341595972896917E-4	0.0	1.0478677095218601E-4	1.435966861196623E-4
42-43	0.04907513772927378	3.363525981181279E-4	0.0	1.1125509014676538E-4	1.6429530754231633E-4
44-45	0.06696004030228578	3.4670190882945493E-4	0.0	1.1125509014676538E-4	1.6946996289797983E-4
46-47	0.08840510575999426	3.699878579299407E-4	0.0	1.1513608166351302E-4	1.7335095441472747E-4
48-49	0.11383983049691931	3.9068647935259473E-4	0.0	1.1901707318026064E-4	1.811129374482227E-4
50-51	0.14422023209001977	4.010357900639217E-4	0.0	1.2289806469700828E-4	1.9016858432063387E-4
52-53	0.17916209237913758	4.1785341996982816E-4	0.0	1.2936638389158766E-4	1.9793056735412912E-4
54-55	0.2182178036760079	4.3725837755356626E-4	0.0	1.3454103924725116E-4	2.1345453342111963E-4
56-57	0.26199668164876005	4.644253181707997E-4	0.0	1.3971569460291468E-4	2.1992285261569902E-4
58-59	0.3103797092242139	4.8383027575453784E-4	0.0	1.4230302228074643E-4	2.2380384413244663E-4
60-61	0.3638170814183119	5.071162248550237E-4	0.0	1.4230302228074643E-4	2.3544681868268953E-4
62-63	0.42124670021930444	5.316958377944252E-4	0.0	1.4489034995857818E-4	2.432088017161848E-4
64-65	0.48459612474717606	5.511007953781634E-4	0.0	1.487713414753258E-4	2.6261375929992293E-4
66-67	0.5520011854100488	5.575691145727427E-4	0.0	1.5265233299207345E-4	2.8589970840040875E-4
68-69	0.6239806414073282	5.717994168008174E-4	0.0	1.5912065218665284E-4	2.9495535527281985E-4
70-71	0.7017194888154611	5.769740721564809E-4	0.0	1.6300164370340044E-4	3.0142367446739924E-4
72-73	0.784424711701192	5.912043743845556E-4	0.0	1.655889713812322E-4	3.0918565750089446E-4
74-75	0.8727069193964893	6.183713150017889E-4	0.0	1.6688263522014808E-4	3.14360312856558E-4
76-77	0.9668067333753912	6.377762725855271E-4	0.0	1.7464461825364333E-4	3.195349682122215E-4
78-79	1.0662493790090157	6.571812301692653E-4	0.0	1.824066012871386E-4	3.234159597289691E-4
80-81	1.1716066557141636	6.70117868558424E-4	0.0	1.8628759280388623E-4	3.2988427892354855E-4
82-83	1.2838669226637667	6.856418346254146E-4	0.0	1.8887492048171798E-4	3.3635259811812794E-4
84-85	1.401845183781378	7.050467922091527E-4	0.0	1.9146224815954973E-4	3.4670190882945493E-4
86-87	1.5266332913470422	7.218644221150591E-4	0.0	1.9663690351521325E-4	3.531702280240343E-4
88	1.6240500594089244	7.425630435377132E-4	0.0	1.9663690351521325E-4	3.596385472186137E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	215745	0.0	82.65083	8
GTGGTAT	219565	0.0	81.4333	6
AGTGGTA	220285	0.0	81.369774	5
TGGTATC	219835	0.0	81.07466	7
GTATCAA	219710	0.0	80.98818	9
AAGCAGT	228190	0.0	79.07986	1
GCAGTGG	234805	0.0	76.8763	3
CAGTGGT	235935	0.0	76.26114	4
AGCAGTG	243410	0.0	74.46675	2
CAACGCA	210445	0.0	44.614613	12-13
ATCAACG	212730	0.0	44.112198	10-11
ACGCAGA	216330	0.0	43.52473	14-15
AGAGTAC	219010	0.0	42.70621	18-19
TCAACGC	209905	0.0	42.26252	12-13
AACGCAG	213590	0.0	41.47838	14-15
AGTACTT	224740	0.0	40.648415	20-21
GAGTACT	211060	0.0	40.618587	20-21
CGCAGAG	218915	0.0	40.46085	16-17
TATCAAC	223855	0.0	39.747433	10-11
GCAGAGT	238770	0.0	39.41748	16-17
>>END_MODULE