FastQCFastQC Report
Thu 26 May 2016
SRR1660583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36112624
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12516443.465945869787806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421700.11677356926486426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1733100.077.897068
GTGGTAT1746150.077.511346
AGTGGTA1750800.077.386645
TGGTATC1748250.077.149447
GTATCAA1756650.076.6869059
AAGCAGT1818300.075.519331
CAGTGGT1891450.071.927274
GCAGTGG1900550.071.821723
AGCAGTG1950900.070.347452
CAACGCA1688650.042.15970212-13
ATCAACG1719100.041.47506710-11
ACGCAGA1729300.041.16382214-15
AGAGTAC1719450.041.01943218-19
AGTACTT1673300.041.0133620-21
GAGTACT1593250.040.46484420-21
TCAACGC1693050.039.63153512-13
AACGCAG1702500.039.2726514-15
GTACTTT1678450.038.38660422-23
TACTTTT1795350.038.37927622-23
CGCAGAG1757000.037.95454816-17
Produced by FastQC (version 0.11.5)