FastQCFastQC Report
Thu 26 May 2016
SRR1660583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36112624
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12752903.5314243573106183No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT375670.10402733404252208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1771250.077.779428
AGTGGTA1795650.077.070555
GTGGTAT1793400.077.062446
TGGTATC1793500.076.780437
GTATCAA1794200.076.567179
AAGCAGT1851450.075.415671
GCAGTGG1926750.072.30453
CAGTGGT1938000.071.6738364
AGCAGTG1985350.070.526082
CAACGCA1722100.042.14697612-13
ATCAACG1747600.041.58038310-11
ACGCAGA1768000.041.05144514-15
AGTACTT1717200.040.96194520-21
AGAGTAC1761950.040.95227418-19
GAGTACT1636650.040.35024320-21
TCAACGC1723500.039.66590512-13
AACGCAG1739150.039.1941414-15
GTACTTT1711250.038.56526622-23
TACTTTT1831400.038.43879722-23
CGCAGAG1796050.037.92236316-17
Produced by FastQC (version 0.11.5)