FastQCFastQC Report
Thu 26 May 2016
SRR1660582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33624873
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15075914.483558941620389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA679410.20205578174228345No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482520.14350091374322813No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT423110.12583244552328868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1944550.082.666188
AGTGGTA1969100.082.016685
GTATCAA1972750.081.3129969
GTGGTAT1983400.081.31236
TGGTATC1981550.081.1060267
AAGCAGT2047400.079.763471
CAGTGGT2101400.077.1265264
GCAGTGG2108800.077.108833
AGCAGTG2203350.074.181032
CAACGCA1895700.044.5145312-13
ATCAACG1931600.043.73801810-11
ACGCAGA1946550.043.41721714-15
AGAGTAC1974150.042.53771218-19
TCAACGC1895650.042.21810512-13
AACGCAG1925450.041.48934614-15
AGTACTT2000550.041.18240420-21
GAGTACT1897850.040.8776820-21
CGCAGAG1961050.040.64978816-17
TATCAAC2010950.039.8718310-11
GCAGAGT2149350.039.28565616-17
Produced by FastQC (version 0.11.5)