FastQCFastQC Report
Thu 26 May 2016
SRR1660582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33624873
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15240804.532597045050549No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT593280.17644081510731655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA554390.1648749721671811No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT448390.13335068953271584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1950000.082.3172158
AGTGGTA1979000.081.4433445
GTGGTAT1987050.081.021196
GTATCAA1976600.080.981229
TGGTATC1981950.080.9665157
AAGCAGT2041650.079.618691
GCAGTGG2106050.076.9630053
CAGTGGT2105600.076.762994
AGCAGTG2202700.073.998972
CAACGCA1900950.044.43039712-13
ATCAACG1937700.043.6471510-11
ACGCAGA1949100.043.41114814-15
AGAGTAC1989350.042.45675718-19
TCAACGC1901600.041.94179512-13
AACGCAG1929600.041.25282714-15
AGTACTT2014900.041.15981720-21
GAGTACT1910350.040.74289320-21
CGCAGAG1971300.040.3384716-17
TATCAAC2012750.039.7505410-11
GCAGAGT2165700.039.1822516-17
Produced by FastQC (version 0.11.5)