FastQCFastQC Report
Thu 26 May 2016
SRR1660581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35242142
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15439014.3808375779202064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA673170.19101279371724908No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488880.13872028550364504No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT398470.1130663397247534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1996750.082.801268
AGTGGTA2024800.081.883035
GTGGTAT2030400.081.5377666
GTATCAA2026050.081.413639
TGGTATC2030000.081.357077
AAGCAGT2125900.078.9416661
GCAGTGG2162550.077.204243
CAGTGGT2164600.076.881234
AGCAGTG2256650.074.368712
CAACGCA1941700.044.6405412-13
ATCAACG1973900.043.93239210-11
ACGCAGA1990800.043.52616514-15
AGAGTAC2018300.042.68424618-19
TCAACGC1940450.042.38710412-13
AACGCAG1970800.041.6540314-15
AGTACTT2051350.041.2073320-21
GAGTACT1948150.040.92590720-21
CGCAGAG2016600.040.6329316-17
TATCAAC2063400.039.9590210-11
GCAGAGT2199150.039.39245616-17
Produced by FastQC (version 0.11.5)