FastQCFastQC Report
Thu 26 May 2016
SRR1660581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35242142
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15697604.454212800118676No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582290.1652254848754653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558960.1586055694344572No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT420300.11926062836929718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2008400.082.611628
AGTGGTA2040150.081.632285
GTGGTAT2042600.081.382546
TGGTATC2041800.081.221127
GTATCAA2041000.081.110229
AAGCAGT2126300.079.0567251
GCAGTGG2181000.076.8624043
CAGTGGT2178400.076.671584
AGCAGTG2271000.074.1775742
CAACGCA1948700.044.55205512-13
ATCAACG1979250.043.8762610-11
ACGCAGA1994950.043.57114-15
AGAGTAC2033950.042.6562618-19
TCAACGC1947050.042.3153212-13
AACGCAG1975250.041.61604314-15
AGTACTT2074150.041.1570920-21
GAGTACT1969800.040.84033620-21
CGCAGAG2016650.040.7174916-17
TATCAAC2068100.039.96347410-11
GCAGAGT2221850.039.27375816-17
Produced by FastQC (version 0.11.5)