FastQCFastQC Report
Thu 26 May 2016
SRR1660580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28803334
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7506642.6061705217875124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA387790.13463371983257216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361330.12544728329019134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1005600.080.180868
GTATCAA1027550.078.252959
AGTGGTA1037900.077.952035
GTGGTAT1039600.077.742916
TGGTATC1041500.077.304187
AAGCAGT1150000.071.2031
CAGTGGT1165700.069.682294
GCAGTGG1176900.069.321243
AGCAGTG1239100.066.124712
CAACGCA958050.044.05030412-13
ATCAACG981700.043.13257210-11
ACGCAGA997400.042.4031714-15
TCAACGC956450.041.99735612-13
AGAGTAC1021050.040.9854118-19
AACGCAG983050.040.7476414-15
CGCAGAG1016250.039.33782216-17
GAGTACT988500.039.25266620-21
TATCAAC1048250.038.37092610-11
AGTACTT1074550.038.27788520-21
GTACTTT1061000.036.52951422-23
Produced by FastQC (version 0.11.5)