FastQCFastQC Report
Thu 26 May 2016
SRR1660580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28803334
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7325832.5433965387479103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA383060.13299154882556305No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350800.12179145650291733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1014650.079.856918
GTATCAA1037000.077.877629
AGTGGTA1047850.077.631545
GTGGTAT1047150.077.575786
TGGTATC1048450.077.354167
AAGCAGT1162350.070.6994551
GCAGTGG1177800.069.49683
CAGTGGT1184000.069.041644
AGCAGTG1256050.065.539912
CAACGCA963050.044.15271812-13
ATCAACG987000.043.0647510-11
ACGCAGA1000200.042.37262714-15
TCAACGC964100.041.7393612-13
AGAGTAC1031150.040.98753718-19
AACGCAG989150.040.58330514-15
CGCAGAG1022200.039.090116-17
GAGTACT1003700.038.945120-21
TATCAAC1055700.038.2713210-11
AGTACTT1088950.038.1610420-21
GTACTTT1070750.036.65452222-23
Produced by FastQC (version 0.11.5)