FastQCFastQC Report
Thu 26 May 2016
SRR1660579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35805626
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9026512.520975334993445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA519340.14504424528145382No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492870.13765155230074738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1221500.079.134728
GTGGTAT1254450.077.189276
AGTGGTA1260850.076.920675
GTATCAA1258050.076.649159
TGGTATC1262350.076.551367
AAGCAGT1409400.069.6967241
GCAGTGG1409450.069.411783
CAGTGGT1409900.069.053344
AGCAGTG1503700.065.2877662
CAACGCA1163250.043.9304412-13
ATCAACG1187500.043.0862510-11
ACGCAGA1214350.042.13883214-15
TCAACGC1164250.041.36489512-13
AGAGTAC1247250.040.57629418-19
AACGCAG1196300.040.159514-15
CGCAGAG1235850.038.8102116-17
GAGTACT1207700.038.65673420-21
TATCAAC1262350.038.22433510-11
AGTACTT1335550.037.27792420-21
GTACTTT1290750.036.17171522-23
Produced by FastQC (version 0.11.5)