##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660579_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35805626 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50416071485526 34.0 31.0 34.0 31.0 34.0 2 32.66700805621999 34.0 31.0 34.0 31.0 34.0 3 32.676820201384 34.0 31.0 34.0 31.0 34.0 4 36.001916374817746 37.0 37.0 37.0 35.0 37.0 5 35.990747012773916 37.0 37.0 37.0 35.0 37.0 6 35.98872163274006 37.0 37.0 37.0 35.0 37.0 7 35.941693297025445 37.0 37.0 37.0 35.0 37.0 8 35.951119301754424 37.0 37.0 37.0 35.0 37.0 9 37.732075456521834 39.0 39.0 39.0 35.0 39.0 10-11 37.66163663777307 39.0 38.0 39.0 35.0 39.0 12-13 37.66878417374968 39.0 38.0 39.0 35.0 39.0 14-15 39.1745293323457 41.0 40.0 41.0 36.0 41.0 16-17 39.12888143611845 41.0 40.0 41.0 36.0 41.0 18-19 39.072158646241796 41.0 40.0 41.0 36.0 41.0 20-21 38.995008577702286 41.0 39.0 41.0 36.0 41.0 22-23 38.865235605711796 41.0 39.0 41.0 35.5 41.0 24-25 38.82317069390157 41.0 39.0 41.0 36.0 41.0 26-27 38.75523970730186 40.0 39.0 41.0 35.5 41.0 28-29 38.66693394775447 40.0 39.0 41.0 35.0 41.0 30-31 38.5552150519586 40.0 39.0 41.0 35.0 41.0 32-33 38.642031534373956 40.0 39.0 41.0 35.0 41.0 34-35 38.58008946694578 40.0 39.0 41.0 35.0 41.0 36-37 38.45755824796919 40.0 38.5 41.0 35.0 41.0 38-39 38.21434592429692 40.0 38.0 41.0 34.5 41.0 40-41 38.17821350197871 40.0 38.0 41.0 34.5 41.0 42-43 38.183229222133974 40.0 38.0 41.0 35.0 41.0 44-45 38.0709234353283 40.0 38.0 41.0 34.0 41.0 46-47 37.94171258170434 40.0 38.0 41.0 34.0 41.0 48-49 37.80982274964275 40.0 38.0 41.0 33.5 41.0 50-51 36.65364542153236 39.0 36.0 40.0 31.5 40.5 52-53 36.71202053554377 39.0 36.5 40.0 32.5 40.5 54-55 37.09547026213143 40.0 37.0 41.0 32.5 41.0 56-57 36.90961709481074 40.0 37.0 41.0 32.0 41.0 58-59 36.703762727120036 40.0 36.0 41.0 32.0 41.0 60-61 36.444999048473555 39.0 35.5 41.0 31.0 41.0 62-63 36.166953260361936 39.0 35.0 41.0 31.0 41.0 64-65 35.87145620635148 39.0 35.0 41.0 31.0 41.0 66-67 35.53203261967826 37.5 35.0 40.0 30.5 41.0 68-69 35.16364101552085 37.0 35.0 40.0 30.0 41.0 70-71 34.816984976048175 36.5 35.0 39.5 30.0 41.0 72-73 34.28079289830039 36.0 35.0 39.0 28.5 41.0 74-75 33.923991525801 35.5 34.0 38.5 28.5 40.5 76-77 33.61224065737602 35.0 34.0 37.0 29.0 39.5 78-79 33.297180281110016 35.0 34.0 37.0 28.5 39.0 80-81 32.97125824584103 35.0 34.0 36.5 28.0 39.0 82-83 32.66895276457393 35.0 34.0 36.0 27.0 37.5 84-85 32.36873456702028 35.0 34.0 36.0 26.5 37.0 86-87 32.08839274308456 35.0 34.0 35.0 26.0 37.0 88-89 31.876552081508084 35.0 33.0 35.0 25.5 36.0 90-91 31.691179578874 35.0 33.0 35.0 25.0 36.0 92-93 31.49588649839553 35.0 33.0 35.0 25.0 36.0 94-95 31.271528097288396 35.0 33.0 35.0 24.0 36.0 96-97 31.066606906970428 35.0 33.0 35.0 23.0 35.0 98-99 30.88280063864824 35.0 33.0 35.0 20.0 35.0 100 30.72266470079311 35.0 33.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42211284121859194 1101 2 0.4184689451317496 1101 3 0.40886433862510785 1101 4 0.3687289591146694 1101 5 0.37322278876616366 1101 6 0.3637063332354131 1101 7 0.393206102505836 1101 8 0.38244125805523765 1101 9 0.45083060123526764 1101 10-11 0.48383465726384145 1101 12-13 0.4705290625754799 1101 14-15 0.5962550727990319 1101 16-17 0.5795039472953718 1101 18-19 0.6059111133211488 1101 20-21 0.5952530751552914 1101 22-23 0.6100858880916107 1101 24-25 0.6260699047534857 1101 26-27 0.6030309735840405 1101 28-29 0.590169144375082 1101 30-31 0.643083787686237 1101 32-33 0.6087904759207419 1101 34-35 0.6134397456721743 1101 36-37 0.6134983421888265 1101 38-39 0.6387361727080361 1101 40-41 0.6578783065927709 1101 42-43 0.677330665428137 1101 44-45 0.6985174911216561 1101 46-47 0.6439154291370954 1101 48-49 0.6670718257284634 1101 50-51 0.6610239336282788 1101 52-53 0.7348790283946371 1101 54-55 0.7070066769661949 1101 56-57 0.7015414876714345 1101 58-59 0.6918824097423837 1101 60-61 0.7136371735482214 1101 62-63 0.6915598734888988 1101 64-65 0.6525896513391629 1101 66-67 0.6694680676592313 1101 68-69 0.6681353509704877 1101 70-71 0.6751609748366434 1101 72-73 0.8001134908342564 1101 74-75 0.7978312354908113 1101 76-77 0.6644989826603549 1101 78-79 0.6730299772759594 1101 80-81 0.6572532893599927 1101 82-83 0.6575316406376857 1101 84-85 0.6409956335729774 1101 86-87 0.6793760090959395 1101 88-89 0.6879141937767308 1101 90-91 0.6930493787651493 1101 92-93 0.7095500146252505 1101 94-95 0.7296935034488925 1101 96-97 0.724392390049772 1101 98-99 0.7264181188490966 1101 100 0.8127934951977096 1102 1 0.4059948664614055 1102 2 0.3684999313094792 1102 3 0.39699319273411504 1102 4 0.3257055543040295 1102 5 0.34075541194295766 1102 6 0.37273110312736435 1102 7 0.38338136187059035 1102 8 0.3730652147247113 1102 9 0.4227801669942366 1102 10-11 0.42145297815820726 1102 12-13 0.4286010592123475 1102 14-15 0.5113603025677875 1102 16-17 0.5335638709512125 1102 18-19 0.5306606887929561 1102 20-21 0.5617105464481895 1102 22-23 0.5634018401729008 1102 24-25 0.5340064544058336 1102 26-27 0.5395461973276383 1102 28-29 0.5408245080308234 1102 30-31 0.5412010414894368 1102 32-33 0.5290511707147303 1102 34-35 0.5357330621133727 1102 36-37 0.5310129658500742 1102 38-39 0.576266099477273 1102 40-41 0.5691462006019989 1102 42-43 0.5799140691221822 1102 44-45 0.6000057758191772 1102 46-47 0.6118429709492261 1102 48-49 0.6113810760906588 1102 50-51 0.5533836498660207 1102 52-53 0.647586605541477 1102 54-55 0.7152300247512855 1102 56-57 0.7536885723669613 1102 58-59 0.7599852467253569 1102 60-61 0.7636975320221637 1102 62-63 0.7981066634342184 1102 64-65 0.7826488570654107 1102 66-67 0.7554509999017185 1102 68-69 0.7321350136631608 1102 70-71 0.7045904521594224 1102 72-73 0.865869203505568 1102 74-75 0.8547856013523472 1102 76-77 0.7503786204013991 1102 78-79 0.7037792959520175 1102 80-81 0.7081845007406855 1102 82-83 0.7020149868403536 1102 84-85 0.7396317316026995 1102 86-87 0.7620460562404006 1102 88-89 0.7537450123402287 1102 90-91 0.7351825458400079 1102 92-93 0.7541917115463335 1102 94-95 0.773054915192148 1102 96-97 0.7431976383334096 1102 98-99 0.7481125627025662 1102 100 0.7821406965446336 1103 1 0.43011743088521115 1103 2 0.41877844085526306 1103 3 0.4117401046230995 1103 4 0.3640116621514835 1103 5 0.3833569676217081 1103 6 0.39511902363849316 1103 7 0.3966264699943167 1103 8 0.4064844218197763 1103 9 0.4579938671920303 1103 10-11 0.46356878619801734 1103 12-13 0.5020913312356754 1103 14-15 0.558049508796735 1103 16-17 0.580702758982568 1103 18-19 0.5896272774734541 1103 20-21 0.6081292254054134 1103 22-23 0.6255385823874704 1103 24-25 0.6156504684220465 1103 26-27 0.6283533331161948 1103 28-29 0.6167605306044379 1103 30-31 0.6435158357590041 1103 32-33 0.6357879313235486 1103 34-35 0.6315666044722335 1103 36-37 0.6449122497393276 1103 38-39 0.6507976848783841 1103 40-41 0.6332493415518101 1103 42-43 0.6481636512855076 1103 44-45 0.6624174089367116 1103 46-47 0.630407082044762 1103 48-49 0.6911249229864964 1103 50-51 0.6671691800969697 1103 52-53 0.7519617841064488 1103 54-55 0.797540589023022 1103 56-57 0.7900678132091059 1103 58-59 0.7761152019345872 1103 60-61 0.786460059333038 1103 62-63 0.7979479290898723 1103 64-65 0.7939399918603698 1103 66-67 0.7948510437506329 1103 68-69 0.8136521965264123 1103 70-71 0.8071434551380889 1103 72-73 0.9189020799698326 1103 74-75 0.8827415599948765 1103 76-77 0.7745323555339709 1103 78-79 0.772247751962972 1103 80-81 0.7527153397241406 1103 82-83 0.7405240549037799 1103 84-85 0.7678007220972844 1103 86-87 0.8027416876640032 1103 88-89 0.7530453753085915 1103 90-91 0.7636890946991706 1103 92-93 0.7735757252809918 1103 94-95 0.7832935349847929 1103 96-97 0.7924742903032573 1103 98-99 0.829905306422404 1103 100 0.8929388053245191 1104 1 0.3933473212926799 1104 2 0.396926602788092 1104 3 0.40151151418452713 1104 4 0.3604361412507231 1104 5 0.36005961199117564 1104 6 0.37872071199399926 1104 7 0.39362218112756153 1104 8 0.3932755652202147 1104 9 0.45446054665891467 1104 10-11 0.47446376400356627 1104 12-13 0.4680942576467544 1104 14-15 0.5327423772864393 1104 16-17 0.5182590831628033 1104 18-19 0.5644172387937232 1104 20-21 0.5722531350878413 1104 22-23 0.5657844023170782 1104 24-25 0.574060989947121 1104 26-27 0.5671490726685278 1104 28-29 0.5808382304738657 1104 30-31 0.5995165348545584 1104 32-33 0.5702313507199506 1104 34-35 0.5632235860144235 1104 36-37 0.5814280039681776 1104 38-39 0.5843508984310049 1104 40-41 0.6197175257566059 1104 42-43 0.6277336625997876 1104 44-45 0.6298992779290344 1104 46-47 0.6462285211171519 1104 48-49 0.6455748555167204 1104 50-51 0.6001540183277072 1104 52-53 0.6234044617427301 1104 54-55 0.6724319324170835 1104 56-57 0.707565471745383 1104 58-59 0.6860975500152975 1104 60-61 0.7159874614096537 1104 62-63 0.701180650479948 1104 64-65 0.700240140620842 1104 66-67 0.6541015386991873 1104 68-69 0.686293726628584 1104 70-71 0.6854978271053795 1104 72-73 0.8368471737845056 1104 74-75 0.7733630918441037 1104 76-77 0.666676906265657 1104 78-79 0.652790126236944 1104 80-81 0.6633300486663813 1104 82-83 0.6588821739546233 1104 84-85 0.6753728606735407 1104 86-87 0.6943749909475869 1104 88-89 0.7202835172932929 1104 90-91 0.7415980797009034 1104 92-93 0.7443339819623773 1104 94-95 0.7426366106164792 1104 96-97 0.7644322613468297 1104 98-99 0.7898746608148883 1104 100 0.82362734659662 1105 1 0.3251576247926451 1105 2 0.3446393222275077 1105 3 0.33813513876425816 1105 4 0.31312918570969117 1105 5 0.324322202332894 1105 6 0.34572876548129017 1105 7 0.33919810015589746 1105 8 0.33241075616309246 1105 9 0.40082705601912494 1105 10-11 0.41186171912809044 1105 12-13 0.3929870198166938 1105 14-15 0.4612904584429742 1105 16-17 0.4791601566949595 1105 18-19 0.4749710543988783 1105 20-21 0.4940389463094732 1105 22-23 0.4973745422285418 1105 24-25 0.4938251927799229 1105 26-27 0.4827638709122084 1105 28-29 0.47506641869751576 1105 30-31 0.494388176097992 1105 32-33 0.46581870361355016 1105 34-35 0.47037258789810465 1105 36-37 0.45756196636546065 1105 38-39 0.5324596484554291 1105 40-41 0.4981569496146747 1105 42-43 0.4726815892958882 1105 44-45 0.5219950679434504 1105 46-47 0.5169748246282353 1105 48-49 0.5668791657611294 1105 50-51 0.3520987776789042 1105 52-53 0.510850196290555 1105 54-55 0.6187790579093573 1105 56-57 0.6461372852686935 1105 58-59 0.6605280445116222 1105 60-61 0.6386056858141984 1105 62-63 0.6495188551235387 1105 64-65 0.657319236699557 1105 66-67 0.6823022666658431 1105 68-69 0.6667959093433851 1105 70-71 0.6111692711033498 1105 72-73 0.7733490281762556 1105 74-75 0.7520317317129823 1105 76-77 0.6292132778953103 1105 78-79 0.59966248017043 1105 80-81 0.5496210437778046 1105 82-83 0.5298630548813819 1105 84-85 0.5449002492822785 1105 86-87 0.6166133891238346 1105 88-89 0.6339161690525934 1105 90-91 0.6555727367805382 1105 92-93 0.6426797280997931 1105 94-95 0.6377978413295864 1105 96-97 0.655818035502822 1105 98-99 0.6576469972563537 1105 100 0.6742539106302878 1106 1 0.3665259871823281 1106 2 0.33439395077657963 1106 3 0.3439289608985163 1106 4 0.3202279092638136 1106 5 0.33507417404894824 1106 6 0.3287690855507108 1106 7 0.340370018711738 1106 8 0.3371715690772419 1106 9 0.40107162897456305 1106 10-11 0.3986615062914396 1106 12-13 0.3971625536074157 1106 14-15 0.4612739380497999 1106 16-17 0.43759909269910224 1106 18-19 0.44684443019142606 1106 20-21 0.4727236117790241 1106 22-23 0.48339732914473643 1106 24-25 0.47215908932828654 1106 26-27 0.4794525072638436 1106 28-29 0.45923953813412055 1106 30-31 0.464704078191744 1106 32-33 0.4524918395328612 1106 34-35 0.4909921308610947 1106 36-37 0.47721360414296754 1106 38-39 0.5153927255334025 1106 40-41 0.4882550638375278 1106 42-43 0.4966979427444045 1106 44-45 0.5057712657072315 1106 46-47 0.48111451231038416 1106 48-49 0.524850466991559 1106 50-51 0.4842528967247759 1106 52-53 0.5501305762554054 1106 54-55 0.5803658142670898 1106 56-57 0.5462763043713466 1106 58-59 0.5671257623804777 1106 60-61 0.604845208275691 1106 62-63 0.5692656399778997 1106 64-65 0.5851466544095061 1106 66-67 0.5920206369857155 1106 68-69 0.5458058461260933 1106 70-71 0.5515708686115914 1106 72-73 0.689396519292913 1106 74-75 0.6623277593038281 1106 76-77 0.5705386571121736 1106 78-79 0.540027677930226 1106 80-81 0.5362082488019553 1106 82-83 0.5274624557857592 1106 84-85 0.5393797020341466 1106 86-87 0.580498532962352 1106 88-89 0.6003331223269406 1106 90-91 0.6054867877479175 1106 92-93 0.6265705828334092 1106 94-95 0.6230806749243882 1106 96-97 0.6093278854271524 1106 98-99 0.6028326006524729 1106 100 0.6588681105492711 1107 1 0.2882375410432658 1107 2 0.2832218747835924 1107 3 0.3056869391533539 1107 4 0.2639571611968421 1107 5 0.2948372738033811 1107 6 0.29500072427345003 1107 7 0.29879976944057063 1107 8 0.309977612140905 1107 9 0.3262231475804498 1107 10-11 0.33577358748200226 1107 12-13 0.35791017370006983 1107 14-15 0.4151345289889292 1107 16-17 0.4098060299860933 1107 18-19 0.4209292022089315 1107 20-21 0.39333020285278053 1107 22-23 0.42317231283182366 1107 24-25 0.40792882086454085 1107 26-27 0.39317721103429903 1107 28-29 0.3940078021593294 1107 30-31 0.4171344096701972 1107 32-33 0.4047639456858434 1107 34-35 0.4173780627699202 1107 36-37 0.3753095930488328 1107 38-39 0.45001005197436683 1107 40-41 0.41955732127745904 1107 42-43 0.416899093242904 1107 44-45 0.45888802515905525 1107 46-47 0.44086565266735533 1107 48-49 0.4723280018239464 1107 50-51 0.3984391732011119 1107 52-53 0.47558279587303787 1107 54-55 0.5233716884171002 1107 56-57 0.5538628653000615 1107 58-59 0.5158868619923638 1107 60-61 0.5268383791165263 1107 62-63 0.5229196770873159 1107 64-65 0.4828791722026793 1107 66-67 0.49175603082749575 1107 68-69 0.4814276046910564 1107 70-71 0.4780577716252026 1107 72-73 0.6318206375588957 1107 74-75 0.5882689976741418 1107 76-77 0.504443060015511 1107 78-79 0.4863191837496359 1107 80-81 0.4988004372673842 1107 82-83 0.4803991543156698 1107 84-85 0.49660746425021784 1107 86-87 0.5241285022386322 1107 88-89 0.5242599385968667 1107 90-91 0.5223879972721868 1107 92-93 0.5358700518667661 1107 94-95 0.5145266544206315 1107 96-97 0.5409847297041424 1107 98-99 0.5203466493370108 1107 100 0.5630591864470986 1108 1 0.2134750917627244 1108 2 0.22268603036807377 1108 3 0.22332070310294938 1108 4 0.22987274311402217 1108 5 0.2117945793810705 1108 6 0.2512757791660647 1108 7 0.2751883310057863 1108 8 0.24689755875421326 1108 9 0.28296588404752043 1108 10-11 0.2742976888218962 1108 12-13 0.27518950933838937 1108 14-15 0.3294712042027683 1108 16-17 0.32164945031620107 1108 18-19 0.3618010598840655 1108 20-21 0.3530562970776643 1108 22-23 0.34920139509397075 1108 24-25 0.3484765896224786 1108 26-27 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-2.0284519462859514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 260756.0 3 48204.0 4 28287.0 5 26367.0 6 28151.0 7 28379.0 8 27764.0 9 31963.0 10 37490.0 11 42583.0 12 45684.0 13 47490.0 14 47602.0 15 47695.0 16 55110.0 17 66382.0 18 86781.0 19 132155.0 20 283325.0 21 319299.0 22 145036.0 23 122855.0 24 136670.0 25 153617.0 26 168399.0 27 195090.0 28 232339.0 29 281474.0 30 346767.0 31 434949.0 32 564413.0 33 758575.0 34 1110785.0 35 1904528.0 36 3730762.0 37 8597133.0 38 1.1679113E7 39 3545046.0 40 6608.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.920245791040582 32.598451973251755 27.350377315052494 23.13092492065516 2 22.09664576890357 29.618059940615556 28.873507665416938 19.41178662506394 3 23.092805541447444 26.835641845100668 27.03296068219661 23.038591931255283 4 20.694942089559596 27.768547011323857 26.864632717599324 24.671878181517222 5 22.384396130612107 29.628275874683517 26.596583844781463 21.390744149922913 6 24.83227569630931 26.689443307857164 27.013602321380354 21.46467867445317 7 21.962822354119382 27.137499960063156 29.442391363506758 21.457286322310708 8 24.016146048788674 26.517203358018037 27.414186137044037 22.052464456149252 9 24.094192499740462 26.08445824819348 27.79693245398524 22.02441679808082 10-11 21.546747275705595 28.218991744915773 28.483114187623276 21.751146791755353 12-13 21.673438915194794 26.88303567477581 28.172444078611747 23.271081331417644 14-15 21.688779320697236 29.792822296427463 26.695855656842472 21.822542726032832 16-17 23.247761335449063 26.755365723647866 26.712352191793702 23.284520749109365 18-19 21.867228085181846 28.211653236498833 26.63281995062194 23.288298727697377 20-21 23.367474309180956 28.131312105475775 26.6125344145654 21.88867917077787 22-23 23.289288662610456 26.766327085474906 28.100020031986695 21.844364219927943 24-25 21.971022811272857 28.00210632367286 26.528530682089603 23.498340182964686 26-27 22.022331528705315 26.523217748989264 29.494199193653937 21.960251528651483 28-29 22.035732445599503 26.60344376312636 29.447798696465714 21.913025094808418 30-31 22.071182925719793 26.55962696376637 29.378434151139725 21.990755959374106 32-33 22.100357389242177 26.56789747153823 29.33223431403607 21.999510825183517 34-35 22.105885757445762 26.630187708510135 29.31302706196947 21.950899472074635 36-37 22.118545240744606 26.603370763120203 29.237876778343725 22.04020721779147 38-39 22.132745890588048 26.541851769139285 29.275785616264884 22.049616724007787 40-41 22.13823030303234 26.61337730513346 29.29159848831933 21.956793903514864 42-43 22.159010328485188 26.577312534251295 29.217191816555815 22.046485320707703 44-45 22.188623706199735 26.554311775693552 29.22120189232636 22.035862625780354 46-47 22.15221386090104 26.639120479022004 29.209191875106434 21.999473784970522 48-49 22.222522618116432 26.561425800817318 29.128429467272298 22.08762211379395 50-51 22.205580417371774 26.576483522656925 29.12543527616464 22.092500783806663 52-53 22.28025157505077 26.68207587997884 28.98297887874637 22.054693666224026 54-55 22.412596055684045 26.786652061558776 28.57743498489175 22.223316897865438 56-57 22.595723424124312 27.01483191555419 28.086505032752644 22.302939627568858 58-59 22.632233869443674 27.254543076701378 27.8525556899211 22.26066736393385 60-61 22.638999596676708 27.320834261446247 27.73037450164316 22.309791640233883 62-63 22.606158781245504 27.369953722621226 27.72634647091867 22.297541025214596 64-65 22.595044311972806 27.410737047286748 27.7666903718292 22.227528268911247 66-67 22.601098671356233 27.261420841430905 27.84970004397592 22.287780443236947 68-69 22.56646202871417 27.13008207638013 28.04696019721481 22.25649569769089 70-71 22.53069194119538 27.108602305250358 28.183862058081026 22.176843695473238 72-73 22.591851010229778 27.070130594844006 28.12216702165438 22.215851373271843 74-75 22.56670896741017 27.06792296091718 28.155610470406927 22.20975760126572 76-77 22.61644353090669 27.189999382914525 28.015707160945162 22.17784992523362 78-79 22.705922666403346 27.22476317862425 27.81999078747456 22.24932336749785 80-81 22.73870703234704 27.298403389230547 27.716077628319624 22.24681195010279 82-83 22.767212045360456 27.434131616664086 27.580887658804524 22.21776867917093 84-85 22.785648638875973 27.468448892160797 27.48709142020596 22.25881104875727 86-87 22.85135623013462 27.450113186217486 27.435684892690166 22.262845690957736 88-89 22.85344352181212 27.536892902815467 27.383712615394735 22.225950959977677 90-91 22.937508478554218 27.508735278178648 27.285413491811976 22.268342751455155 92-93 22.962801425981343 27.497507113914093 27.277863788816497 22.261827671288067 94-95 22.98415570241431 27.574752019078712 27.25243567358502 22.18865660492196 96-97 23.031172099425174 27.536541909390355 27.1928358535292 22.239450137655272 98-99 23.035110274965646 27.50303211867341 27.222346365579465 22.23951124078148 100 23.051979733912674 27.578432918575725 27.168797088987397 22.200790258524208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 171926.0 1 106704.0 2 30280.0 3 16315.0 4 11602.5 5 8566.5 6 7212.0 7 6802.0 8 6543.0 9 6538.0 10 7210.5 11 11042.0 12 95765.0 13 111869.0 14 44488.0 15 45129.0 16 51073.5 17 57998.5 18 64715.5 19 71198.5 20 81401.0 21 97641.5 22 120203.0 23 148848.0 24 182370.5 25 220714.5 26 265185.0 27 318297.5 28 383033.0 29 447960.0 30 502767.5 31 552732.0 32 606389.5 33 660828.0 34 711640.5 35 769972.0 36 841097.5 37 901428.0 38 950060.5 39 993581.0 40 1020660.5 41 1050267.5 42 1093053.5 43 1123580.0 44 1155795.0 45 1197127.5 46 1224674.5 47 1242491.0 48 1244114.5 49 1236459.5 50 1233191.0 51 1228471.0 52 1216986.0 53 1190565.5 54 1148345.5 55 1098501.5 56 1043566.5 57 974606.0 58 881231.0 59 769656.5 60 644699.0 61 522917.0 62 416022.0 63 318740.0 64 237053.0 65 174558.5 66 127824.5 67 95038.0 68 70817.5 69 53324.0 70 41161.0 71 31564.0 72 24695.5 73 18917.0 74 14345.5 75 11283.5 76 8501.5 77 5571.5 78 3556.0 79 2453.5 80 1609.5 81 1109.5 82 772.5 83 536.0 84 403.5 85 319.5 86 264.0 87 216.5 88 174.0 89 141.0 90 111.5 91 87.5 92 77.0 93 68.5 94 51.0 95 35.0 96 30.5 97 27.0 98 19.0 99 12.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14842080962360496 2 0.24066888259403704 3 0.2551191256927054 4 0.2830728333027888 5 0.29380578348218234 6 0.32926110550336424 7 0.3471298058020268 8 0.3569131845369775 9 0.3548995345033208 10-11 0.36554032039545964 12-13 0.3747721098354767 14-15 0.36710571685019555 16-17 0.371815870500351 18-19 0.3751281991271428 20-21 0.380715309934813 22-23 0.3844954421408524 24-25 0.38748938504803687 26-27 0.3897432207999938 28-29 0.3917847994055459 30-31 0.3832945135493512 32-33 0.4008546031285698 34-35 0.41051369971858614 36-37 0.41326885333606517 38-39 0.4096046247033916 40-41 0.4139782390622077 42-43 0.4139544997760966 44-45 0.417867292698639 46-47 0.42702926070891767 48-49 0.41607008909717147 50-51 0.4220188749108869 52-53 0.4283195607304841 54-55 0.42803050001136694 56-57 0.4226444749213434 58-59 0.43160396078538055 60-61 0.4275459392889821 62-63 0.4158452640934137 64-65 0.43727765016592646 66-67 0.4372692715943578 68-69 0.44409920385137236 70-71 0.4428494002590542 72-73 0.4534859968654088 74-75 0.46137023271147387 76-77 0.4531843682889387 78-79 0.4426846216848715 80-81 0.4449161145793122 82-83 0.44992091466296386 84-85 0.4280682035834257 86-87 0.447932400344013 88-89 0.44425420742539173 90-91 0.45867372909497517 92-93 0.4586039076652368 94-95 0.4621759720106556 96-97 0.46934523641619896 98-99 0.4818753901970601 100 0.48453000095571575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5805626E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.24631080300978 #Duplication Level Percentage of deduplicated Percentage of total 1 67.45357671881482 27.14757613400085 2 15.470330330689 12.45247445428277 3 5.948513923098405 7.182172204951481 4 3.067146042802108 4.937652516673406 5 1.8159431950160083 3.6542507113612435 6 1.2093066472304255 2.920207870834884 7 0.8817783358810288 2.4841827475660043 8 0.632778790597842 2.037360950076271 9 0.4838487351053101 1.752581391722231 >10 2.7378791027923937 20.079875897123227 >50 0.20726981584513654 5.7849220521555305 >100 0.08990663536773282 6.039263877280028 >500 0.0012876317867900088 0.34259269683520527 >1k 3.9221381625980295E-4 0.2520283393693323 >5k 1.3960386909462045E-5 0.03350335744755054 >10k+ 2.792076971159494E-5 2.899354798319973 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 902651 2.520975334993445 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51934 0.14504424528145382 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49287 0.13765155230074738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1171428758151024E-4 8.378571568613267E-6 0.0 5.585714379075512E-6 0.0 2 1.2009285915012351E-4 1.396428594768878E-5 0.0 5.585714379075512E-6 0.0 3 1.396428594768878E-4 2.5135714705839804E-5 0.0 5.585714379075512E-6 2.792857189537756E-6 4 1.5919285980365208E-4 3.9100000653528584E-5 0.0 5.585714379075512E-6 2.792857189537756E-6 5 1.647785741827276E-4 4.747857222214185E-5 0.0 5.585714379075512E-6 2.792857189537756E-6 6 1.7315714575134086E-4 5.585714379075512E-5 0.0 5.585714379075512E-6 5.585714379075512E-6 7 1.7315714575134086E-4 6.144285816983063E-5 0.0 8.378571568613267E-6 5.585714379075512E-6 8 1.8153571731995414E-4 6.702857254890614E-5 0.0 1.1171428758151023E-5 5.585714379075512E-6 9 1.8153571731995414E-4 7.261428692798165E-5 0.0 1.396428594768878E-5 8.378571568613267E-6 10-11 1.9270714607810515E-4 8.099285849659491E-5 4.1892857843066335E-6 1.396428594768878E-5 8.378571568613267E-6 12-13 2.2482500375778933E-4 9.635357303905257E-5 1.1171428758151023E-5 1.396428594768878E-5 9.775000163382146E-6 14-15 2.5694286143747357E-4 1.0473214460766583E-4 1.1171428758151023E-5 1.396428594768878E-5 1.1171428758151023E-5 16-17 2.75096433169469E-4 1.14507144771048E-4 1.6757143137226534E-5 1.396428594768878E-5 1.5360714542457655E-5 18-19 3.0721429084915313E-4 1.2986785931350564E-4 1.6757143137226534E-5 1.6757143137226534E-5 1.8153571731995413E-5 20-21 3.3514286274453073E-4 1.5360714542457656E-4 1.6757143137226534E-5 1.9550000326764292E-5 1.9550000326764292E-5 22-23 3.588821488556016E-4 1.7455357434610974E-4 1.6757143137226534E-5 1.9550000326764292E-5 1.9550000326764292E-5 24-25 4.0077500669866796E-4 1.9270714607810515E-4 2.094642892153317E-5 1.9550000326764292E-5 2.2342857516302046E-5 26-27 5.501928663389379E-4 2.1086071781010058E-4 2.653214330060868E-5 1.9550000326764292E-5 2.2342857516302046E-5 28-29 8.909214434625441E-4 2.276178609473271E-4 2.792857189537756E-5 1.9550000326764292E-5 2.5135714705839804E-5 30-31 0.001612875026958054 2.611321472217802E-4 2.792857189537756E-5 1.9550000326764292E-5 2.653214330060868E-5 32-33 0.0031796679102887348 2.8487143333285106E-4 2.792857189537756E-5 1.9550000326764292E-5 3.77035720587597E-5 34-35 0.006017210814859095 3.0162857647007764E-4 2.792857189537756E-5 1.9550000326764292E-5 4.468571503260409E-5 36-37 0.01036708588756415 3.1280000522822867E-4 2.792857189537756E-5 1.9550000326764292E-5 4.747857222214185E-5 38-39 0.01691493956843542 3.3793571993406845E-4 2.9325000490146438E-5 2.2342857516302046E-5 5.446071519598624E-5 40-41 0.025755729001917187 3.477107200974506E-4 3.2117857679684196E-5 2.2342857516302046E-5 5.8650000980292876E-5 42-43 0.03701373633294387 3.5469286307129497E-4 3.7703572058759705E-5 2.2342857516302046E-5 6.0046429575061755E-5 44-45 0.05120284728439045 3.5469286307129497E-4 4.1892857843066335E-5 2.3739286111070925E-5 6.28392867645995E-5 46-47 0.06885649757945861 3.6726072042421486E-4 4.1892857843066335E-5 2.653214330060868E-5 6.842500114367502E-5 48-49 0.09026514436586028 3.8122500637190367E-4 4.608214362737297E-5 3.351428627445307E-5 6.98214297384439E-5 50-51 0.11521513406859582 4.021714352934368E-4 4.747857222214185E-5 3.351428627445307E-5 7.121785833321277E-5 52-53 0.14411701669452726 4.301000071888144E-4 4.887500081691073E-5 3.491071486922195E-5 7.959642990182604E-5 54-55 0.1772919708204515 4.552357218946542E-4 5.027142941167961E-5 3.9100000653528584E-5 8.378571568613267E-5 56-57 0.21461012858705503 4.7757857941095627E-4 5.027142941167961E-5 4.0496429248297456E-5 8.378571568613267E-5 58-59 0.25589833284858643 5.166785800644849E-4 5.027142941167961E-5 4.1892857843066335E-5 8.657857287567043E-5 60-61 0.3021871479079852 5.446071519598623E-4 5.027142941167961E-5 4.3289286437835214E-5 9.076785865997706E-5 62-63 0.3531847201889446 5.781214382343154E-4 5.166785800644849E-5 4.468571503260409E-5 9.775000163382146E-5 64-65 0.4086606389733278 6.130321531035375E-4 5.306428660121736E-5 4.468571503260409E-5 1.0054285882335922E-4 66-67 0.4691734756990424 6.283928676459951E-4 5.306428660121736E-5 4.468571503260409E-5 1.0473214460766583E-4 68-69 0.5340948374984422 6.437535821884528E-4 5.585714379075512E-5 4.468571503260409E-5 1.1171428758151024E-4 70-71 0.6036802708043703 6.563214395413726E-4 6.144285816983063E-5 4.468571503260409E-5 1.14507144771048E-4 72-73 0.6791907506379025 6.702857254890615E-4 6.423571535936839E-5 4.608214362737297E-5 1.1730000196058575E-4 74-75 0.7598708091292692 6.954214401949012E-4 6.702857254890614E-5 5.027142941167961E-5 1.2009285915012351E-4 76-77 0.8466909641518348 7.177642977112032E-4 6.702857254890614E-5 5.027142941167961E-5 1.2148928774489238E-4 78-79 0.9403717728604997 7.540714411751941E-4 6.702857254890614E-5 5.027142941167961E-5 1.25678573529199E-4 80-81 1.0400181245260174 7.820000130705717E-4 6.702857254890614E-5 5.027142941167961E-5 1.326607165030434E-4 82-83 1.1458478620091714 8.169107279397936E-4 6.98214297384439E-5 5.306428660121736E-5 1.396428594768878E-4 84-85 1.2595213389091424 8.560107285933221E-4 6.98214297384439E-5 5.306428660121736E-5 1.4383214526119445E-4 86-87 1.3802020944976636 8.769571575148554E-4 7.261428692798165E-5 5.306428660121736E-5 1.508142882350388E-4 88 1.475282124658287 8.937143006520819E-4 7.261428692798165E-5 5.306428660121736E-5 1.5360714542457656E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 122150 0.0 79.13472 8 GTGGTAT 125445 0.0 77.18927 6 AGTGGTA 126085 0.0 76.92067 5 GTATCAA 125805 0.0 76.64915 9 TGGTATC 126235 0.0 76.55136 7 AAGCAGT 140940 0.0 69.696724 1 GCAGTGG 140945 0.0 69.41178 3 CAGTGGT 140990 0.0 69.05334 4 AGCAGTG 150370 0.0 65.287766 2 CAACGCA 116325 0.0 43.93044 12-13 ATCAACG 118750 0.0 43.08625 10-11 ACGCAGA 121435 0.0 42.138832 14-15 TCAACGC 116425 0.0 41.364895 12-13 AGAGTAC 124725 0.0 40.576294 18-19 AACGCAG 119630 0.0 40.1595 14-15 CGCAGAG 123585 0.0 38.81021 16-17 GAGTACT 120770 0.0 38.656734 20-21 TATCAAC 126235 0.0 38.224335 10-11 AGTACTT 133555 0.0 37.277924 20-21 GTACTTT 129075 0.0 36.171715 22-23 >>END_MODULE