FastQCFastQC Report
Thu 26 May 2016
SRR1660579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35805626
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8820792.4635206768902744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510860.1426759023847258No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483260.13496761654160158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1221200.078.925398
GTGGTAT1252400.077.127966
GTATCAA1249450.077.0394069
AGTGGTA1257900.076.906695
TGGTATC1256700.076.6173867
AAGCAGT1409500.069.2714161
CAGTGGT1408000.068.9816744
GCAGTGG1412650.068.887513
AGCAGTG1518400.064.30942
CAACGCA1158000.043.95586412-13
ATCAACG1182150.043.0664110-11
ACGCAGA1204550.042.1973414-15
TCAACGC1157050.041.42854712-13
AGAGTAC1247400.040.599218-19
AACGCAG1191850.040.1997614-15
CGCAGAG1227950.038.8294816-17
GAGTACT1201750.038.6803320-21
TATCAAC1256950.038.29519710-11
AGTACTT1329750.037.3434120-21
GTACTTT1291450.036.16745822-23
Produced by FastQC (version 0.11.5)