FastQCFastQC Report
Thu 26 May 2016
SRR1660578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34500540
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9126492.645318015312224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500470.14506149758815368No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459410.13316023459342957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1223650.079.782868
AGTGGTA1248450.078.425115
GTATCAA1245400.078.1713269
GTGGTAT1255300.077.886736
TGGTATC1264050.077.157847
AAGCAGT1402300.070.771971
GCAGTGG1406050.070.309953
CAGTGGT1415750.069.380024
AGCAGTG1514750.065.5767062
CAACGCA1161850.044.1398412-13
ATCAACG1194400.043.01889810-11
ACGCAGA1217000.042.22186714-15
TCAACGC1166100.041.7392312-13
AGAGTAC1239600.041.0376618-19
AACGCAG1195500.040.61582614-15
GAGTACT1191750.039.47477320-21
CGCAGAG1236850.039.24891716-17
TATCAAC1255300.038.8082810-11
AGTACTT1301900.038.39753320-21
GTACTTT1295600.036.40768422-23
Produced by FastQC (version 0.11.5)