##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660578_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34500540 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.509990684203785 34.0 31.0 34.0 31.0 34.0 2 32.670698081827126 34.0 31.0 34.0 31.0 34.0 3 32.68020025193808 34.0 31.0 34.0 31.0 34.0 4 35.99732702734508 37.0 37.0 37.0 35.0 37.0 5 35.985619790298934 37.0 37.0 37.0 35.0 37.0 6 35.98564732609982 37.0 37.0 37.0 35.0 37.0 7 35.93707994135744 37.0 37.0 37.0 35.0 37.0 8 35.94921015729029 37.0 37.0 37.0 35.0 37.0 9 37.73380825343603 39.0 39.0 39.0 35.0 39.0 10-11 37.66038718524406 39.0 38.5 39.0 35.0 39.0 12-13 37.668782879920144 39.0 38.5 39.0 35.0 39.0 14-15 39.181994499216536 41.0 40.0 41.0 37.0 41.0 16-17 39.13655339887434 41.0 40.0 41.0 36.0 41.0 18-19 39.080660070827875 41.0 40.0 41.0 36.0 41.0 20-21 39.002517221469574 41.0 39.5 41.0 36.0 41.0 22-23 38.869466840229165 41.0 39.0 41.0 35.5 41.0 24-25 38.82853223456792 41.0 39.0 41.0 36.0 41.0 26-27 38.76277411889785 40.0 39.0 41.0 36.0 41.0 28-29 38.67749594644026 40.0 39.0 41.0 35.0 41.0 30-31 38.56495950498166 40.0 39.0 41.0 35.0 41.0 32-33 38.650284966554146 40.0 39.0 41.0 35.0 41.0 34-35 38.58880176947955 40.0 39.0 41.0 35.0 41.0 36-37 38.46463975636323 40.0 38.5 41.0 35.0 41.0 38-39 38.21501462006102 40.0 38.0 41.0 35.0 41.0 40-41 38.17873926031302 40.0 38.0 41.0 34.5 41.0 42-43 38.183811934537836 40.0 38.0 41.0 35.0 41.0 44-45 38.06857010064191 40.0 38.0 41.0 34.0 41.0 46-47 37.94019719691344 40.0 38.0 41.0 34.0 41.0 48-49 37.80985225158794 40.0 38.0 41.0 34.0 41.0 50-51 36.65372282578765 39.0 36.0 40.0 31.5 40.5 52-53 36.70459536285519 39.0 36.5 40.0 32.5 40.5 54-55 37.07292040936171 40.0 37.0 41.0 32.5 41.0 56-57 36.87548018088992 40.0 37.0 41.0 32.0 41.0 58-59 36.664360673774965 40.0 36.0 41.0 32.0 41.0 60-61 36.40198166463482 39.0 35.5 41.0 31.0 41.0 62-63 36.118939355731825 39.0 35.0 41.0 31.0 41.0 64-65 35.81905648433329 38.5 35.0 41.0 31.0 41.0 66-67 35.47607788167953 37.5 35.0 40.0 30.5 41.0 68-69 35.10450450920479 37.0 35.0 40.0 30.0 41.0 70-71 34.75437485906018 36.5 35.0 39.0 30.0 41.0 72-73 34.21833352463469 36.0 35.0 39.0 28.5 41.0 74-75 33.859544792632235 35.5 34.0 38.5 28.5 40.0 76-77 33.54953515220342 35.0 34.0 37.0 29.0 39.5 78-79 33.23602569698909 35.0 34.0 37.0 28.5 39.0 80-81 32.913462919710824 35.0 34.0 36.5 27.5 39.0 82-83 32.61573666673043 35.0 34.0 36.0 27.0 37.5 84-85 32.32368709301362 35.0 34.0 36.0 26.5 37.0 86-87 32.054251904462944 35.0 34.0 35.0 26.0 37.0 88-89 31.847668775619162 35.0 33.0 35.0 25.5 36.0 90-91 31.665125661801234 35.0 33.0 35.0 25.0 36.0 92-93 31.47598186868959 35.0 33.0 35.0 25.0 36.0 94-95 31.257484404591928 35.0 33.0 35.0 24.0 35.5 96-97 31.058049671106595 35.0 33.0 35.0 23.0 35.0 98-99 30.87902673407431 35.0 33.0 35.0 20.0 35.0 100 30.722656862762147 35.0 33.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4115894255989687 1101 2 0.40479857866668567 1101 3 0.43106030525605377 1101 4 0.34347457601078446 1101 5 0.3419489461429066 1101 6 0.35058824705629377 1101 7 0.394812928717478 1101 8 0.35688991387738866 1101 9 0.4557398120334639 1101 10-11 0.47018949773008956 1101 12-13 0.4579378341039728 1101 14-15 0.561369233763827 1101 16-17 0.5465366844402482 1101 18-19 0.5595466233890605 1101 20-21 0.5639815258723999 1101 22-23 0.5810488785068131 1101 24-25 0.5716269469240203 1101 26-27 0.581321737079449 1101 28-29 0.5842921760359943 1101 30-31 0.5688212879733143 1101 32-33 0.5752885120047893 1101 34-35 0.5882020699545834 1101 36-37 0.5871257276444339 1101 38-39 0.6139028068997305 1101 40-41 0.5901727611237888 1101 42-43 0.6233976453507779 1101 44-45 0.649425798084188 1101 46-47 0.6187290222840716 1101 48-49 0.6277540702794369 1101 50-51 0.629098481045034 1101 52-53 0.7145993505480206 1101 54-55 0.7203141731013645 1101 56-57 0.7258191208242479 1101 58-59 0.7580941303187458 1101 60-61 0.7477968799335528 1101 62-63 0.731140868098862 1101 64-65 0.7102667967329381 1101 66-67 0.7223956849754529 1101 68-69 0.741980468181545 1101 70-71 0.7403900554443155 1101 72-73 0.8464226344616677 1101 74-75 0.8166585284085102 1101 76-77 0.7163392277798337 1101 78-79 0.7159544927023873 1101 80-81 0.6917145311770412 1101 82-83 0.6774319759105651 1101 84-85 0.6906678133134392 1101 86-87 0.7444152656436742 1101 88-89 0.7226769790664598 1101 90-91 0.6902423290061677 1101 92-93 0.7487451273273962 1101 94-95 0.767252412318097 1101 96-97 0.7833916836256023 1101 98-99 0.8019910140002366 1101 100 0.8784495260116856 1102 1 0.3843580539533207 1102 2 0.36857056929948584 1102 3 0.39224009986571673 1102 4 0.3053856076039807 1102 5 0.3138926378908664 1102 6 0.3319655698393902 1102 7 0.3398208413004582 1102 8 0.34602324468572476 1102 9 0.409143738753194 1102 10-11 0.40815317230119064 1102 12-13 0.4177205919021887 1102 14-15 0.5001163880840735 1102 16-17 0.521848715894933 1102 18-19 0.506918747126285 1102 20-21 0.5310724422868915 1102 22-23 0.5200992886582654 1102 24-25 0.5089852122261149 1102 26-27 0.5291670515411653 1102 28-29 0.5148202309031333 1102 30-31 0.5364659659547399 1102 32-33 0.548980467672763 1102 34-35 0.5377151553098471 1102 36-37 0.5326767127745171 1102 38-39 0.5583242362863601 1102 40-41 0.5488768815463203 1102 42-43 0.5420943254342916 1102 44-45 0.6037168310566727 1102 46-47 0.5691418116927167 1102 48-49 0.555881253499912 1102 50-51 0.5161541923094859 1102 52-53 0.5719820932859463 1102 54-55 0.6150250192007292 1102 56-57 0.6412886461327005 1102 58-59 0.6268341805414082 1102 60-61 0.6150029478594874 1102 62-63 0.6123284615809652 1102 64-65 0.6473714230608039 1102 66-67 0.6262702145798187 1102 68-69 0.6382982223792197 1102 70-71 0.5945008168118235 1102 72-73 0.7661654522959367 1102 74-75 0.7617971611964975 1102 76-77 0.6195503240782543 1102 78-79 0.5772944652991612 1102 80-81 0.5974771928642184 1102 82-83 0.5861969018344055 1102 84-85 0.6227264700585664 1102 86-87 0.6554854960072234 1102 88-89 0.6439728837891714 1102 90-91 0.6605308789401789 1102 92-93 0.664579585278716 1102 94-95 0.6873540138051588 1102 96-97 0.6945857741794335 1102 98-99 0.7021950336762188 1102 100 0.7354710728006957 1103 1 0.40230576583720534 1103 2 0.38087652471453737 1103 3 0.3698459488008936 1103 4 0.33395484689950194 1103 5 0.3449852409379375 1103 6 0.35889287578818596 1103 7 0.36392905515203466 1103 8 0.3557168486671003 1103 9 0.42729566775290806 1103 10-11 0.4076690717319451 1103 12-13 0.4435446365962221 1103 14-15 0.506530563199675 1103 16-17 0.5150718849609888 1103 18-19 0.5402868362890771 1103 20-21 0.5437185121858548 1103 22-23 0.5596485304277721 1103 24-25 0.5523972885707664 1103 26-27 0.5782351530122583 1103 28-29 0.5774251996783235 1103 30-31 0.5828585895640899 1103 32-33 0.5674302947974752 1103 34-35 0.5509335202328813 1103 36-37 0.5929993378306619 1103 38-39 0.5714427536244457 1103 40-41 0.5405828253332459 1103 42-43 0.5822138716911596 1103 44-45 0.6223979212822357 1103 46-47 0.6201643560554047 1103 48-49 0.6483961304897647 1103 50-51 0.5851998798342066 1103 52-53 0.6784849999553657 1103 54-55 0.7247489743070901 1103 56-57 0.7217374564673733 1103 58-59 0.6954607303758777 1103 60-61 0.6949572662686379 1103 62-63 0.6671699669516045 1103 64-65 0.6590377912046179 1103 66-67 0.654425174604043 1103 68-69 0.6777799344836737 1103 70-71 0.6254324662563491 1103 72-73 0.7814532332587376 1103 74-75 0.738099262838162 1103 76-77 0.6264499047753063 1103 78-79 0.629132667451195 1103 80-81 0.6237633604640038 1103 82-83 0.6267191371246028 1103 84-85 0.6431260247355652 1103 86-87 0.6536338888640856 1103 88-89 0.6454217177807315 1103 90-91 0.6779877057636128 1103 92-93 0.6976577683370735 1103 94-95 0.705820595399917 1103 96-97 0.7068988592063867 1103 98-99 0.7694715405158803 1103 100 0.8392589723167809 1104 1 0.37966122127245683 1104 2 0.3856858319306511 1104 3 0.4119129429011892 1104 4 0.36106195476808267 1104 5 0.36014305242113664 1104 6 0.36115445597574336 1104 7 0.39219754693110076 1104 8 0.3812808326601953 1104 9 0.4395527789139493 1104 10-11 0.4588883025527579 1104 12-13 0.4360542087541006 1104 14-15 0.5068116228125206 1104 16-17 0.5128611200972202 1104 18-19 0.5661260477191874 1104 20-21 0.5734137990905097 1104 22-23 0.5761437298261001 1104 24-25 0.57419874572917 1104 26-27 0.5372305936866937 1104 28-29 0.5467811042525668 1104 30-31 0.5408603989505494 1104 32-33 0.5218924261216031 1104 34-35 0.5263290018593523 1104 36-37 0.5383924942011689 1104 38-39 0.5582761932599638 1104 40-41 0.6037539710979516 1104 42-43 0.5791006424451766 1104 44-45 0.6012383751891335 1104 46-47 0.6243148672253085 1104 48-49 0.6380011996449184 1104 50-51 0.577841126364234 1104 52-53 0.6567953958652808 1104 54-55 0.6898068680726865 1104 56-57 0.7235450937236507 1104 58-59 0.7313425333871422 1104 60-61 0.7441658443557557 1104 62-63 0.7408966180058059 1104 64-65 0.7130696851213827 1104 66-67 0.6918954323809317 1104 68-69 0.7078424955787455 1104 70-71 0.6910031529198548 1104 72-73 0.8384452674268701 1104 74-75 0.8113919346593264 1104 76-77 0.7151598981700644 1104 78-79 0.6844756557779874 1104 80-81 0.6747069276711954 1104 82-83 0.6742677049997283 1104 84-85 0.6844913755580393 1104 86-87 0.6935083835579903 1104 88-89 0.7136673886200207 1104 90-91 0.7638319978867258 1104 92-93 0.7710887710949663 1104 94-95 0.7654042920213975 1104 96-97 0.7679311777703042 1104 98-99 0.8100697885944115 1104 100 0.8534359770034854 1105 1 0.36849273752643796 1105 2 0.36491322108374646 1105 3 0.3650486871534042 1105 4 0.3412773714198849 1105 5 0.347512509328439 1105 6 0.3592930913375838 1105 7 0.365628590548539 1105 8 0.3556683538363643 1105 9 0.4072859832830886 1105 10-11 0.42913447682320793 1105 12-13 0.419127453224327 1105 14-15 0.4948683866255976 1105 16-17 0.5128462383330898 1105 18-19 0.529019553206858 1105 20-21 0.5225354353157741 1105 22-23 0.5386077969386278 1105 24-25 0.5486140326525728 1105 26-27 0.5402693972846038 1105 28-29 0.5246466122095086 1105 30-31 0.546758271309379 1105 32-33 0.5324134678983299 1105 34-35 0.5217400306345255 1105 36-37 0.5184373985368964 1105 38-39 0.5629048998723292 1105 40-41 0.532486929763202 1105 42-43 0.5336718694197558 1105 44-45 0.6014705132545757 1105 46-47 0.5635235957671156 1105 48-49 0.5788022755851401 1105 50-51 0.39651416970795594 1105 52-53 0.5320529981387381 1105 54-55 0.5984811901053746 1105 56-57 0.6245693046506702 1105 58-59 0.6668964462984874 1105 60-61 0.6496756819082279 1105 62-63 0.6286255074951796 1105 64-65 0.6337375390127278 1105 66-67 0.6276072913610804 1105 68-69 0.6354733514025881 1105 70-71 0.6093484922626473 1105 72-73 0.755677567270304 1105 74-75 0.7386249286396165 1105 76-77 0.6355057827148087 1105 78-79 0.617682564618093 1105 80-81 0.5834491919868725 1105 82-83 0.576927174707258 1105 84-85 0.5805960302272197 1105 86-87 0.6594059482994581 1105 88-89 0.6685675683109444 1105 90-91 0.6646434355876281 1105 92-93 0.6526694335334753 1105 94-95 0.641585236643671 1105 96-97 0.630270441933245 1105 98-99 0.6217990877966351 1105 100 0.6654835874993168 1106 1 0.3398217516432922 1106 2 0.3141984114774772 1106 3 0.33246111411301627 1106 4 0.31762819232343986 1106 5 0.3201442615929224 1106 6 0.32055831355154396 1106 7 0.3454587931052657 1106 8 0.344498825361363 1106 9 0.3773876701440031 1106 10-11 0.39440721665814493 1106 12-13 0.3895124754754349 1106 14-15 0.4423045929723699 1106 16-17 0.45042768205219375 1106 18-19 0.45666354103970974 1106 20-21 0.4786952465612373 1106 22-23 0.48305952082230164 1106 24-25 0.4694462087934639 1106 26-27 0.48734014023180805 1106 28-29 0.496045439306279 1106 30-31 0.4931680911540397 1106 32-33 0.4554504778580508 1106 34-35 0.49213296951104724 1106 36-37 0.4609731152529051 1106 38-39 0.49240600042441685 1106 40-41 0.47316142331304434 1106 42-43 0.4860699924883036 1106 44-45 0.48188607632709335 1106 46-47 0.48749550028018973 1106 48-49 0.5434487180649725 1106 50-51 0.45444219070319747 1106 52-53 0.5244345463695907 1106 54-55 0.5329360341645639 1106 56-57 0.5282536184321245 1106 58-59 0.5415436094114412 1106 60-61 0.5732388886734512 1106 62-63 0.5492412000979741 1106 64-65 0.5555555812563924 1106 66-67 0.5329759046854718 1106 68-69 0.514376126968827 1106 70-71 0.5311881331734085 1106 72-73 0.666996302835706 1106 74-75 0.6349612388625019 1106 76-77 0.5443343346259653 1106 78-79 0.5121259876825661 1106 80-81 0.5233199024459836 1106 82-83 0.5196077282152274 1106 84-85 0.5126346215872957 1106 86-87 0.5758220238779543 1106 88-89 0.5668213562276883 1106 90-91 0.5507669947489404 1106 92-93 0.5509182477592418 1106 94-95 0.5664860946337207 1106 96-97 0.5524123173815703 1106 98-99 0.5565835204390126 1106 100 0.6004762219322544 1107 1 0.2933060426013583 1107 2 0.28176828458530423 1107 3 0.3110903714054416 1107 4 0.28372612370247907 1107 5 0.2875398181445874 1107 6 0.29979194957515887 1107 7 0.31137880241477944 1107 8 0.30383799430610026 1107 9 0.3517966421690275 1107 10-11 0.3441577868334349 1107 12-13 0.35486420671161767 1107 14-15 0.41774413407391364 1107 16-17 0.42594911883907827 1107 18-19 0.4376659476880178 1107 20-21 0.4329224098461637 1107 22-23 0.4306224104786551 1107 24-25 0.4241610211760971 1107 26-27 0.4089693631944584 1107 28-29 0.4074067963890897 1107 30-31 0.417498256763146 1107 32-33 0.4342304337730951 1107 34-35 0.4234639517825869 1107 36-37 0.39674000227910966 1107 38-39 0.4234833744363371 1107 40-41 0.4095215424801779 1107 42-43 0.4383095004409867 1107 44-45 0.47166222661822843 1107 46-47 0.4307110751947505 1107 48-49 0.4485484319743236 1107 50-51 0.3867765402283254 1107 52-53 0.4535367398068644 1107 54-55 0.5145810416277214 1107 56-57 0.5173301705235644 1107 58-59 0.4825229978159413 1107 60-61 0.5119670801146654 1107 62-63 0.5531669645443174 1107 64-65 0.5141397491002948 1107 66-67 0.5122493390667486 1107 68-69 0.48508346136193836 1107 70-71 0.4887030921729618 1107 72-73 0.6659731735448204 1107 74-75 0.5943072034260339 1107 76-77 0.48896050974164496 1107 78-79 0.47164056234895924 1107 80-81 0.4722165953238999 1107 82-83 0.4505873072293838 1107 84-85 0.45866498255012544 1107 86-87 0.4836931802840425 1107 88-89 0.5005943112782738 1107 90-91 0.5336898095803306 1107 92-93 0.5101611672514039 1107 94-95 0.4841000537920941 1107 96-97 0.47212286663159375 1107 98-99 0.4809387732738841 1107 100 0.523975183551233 1108 1 0.2321696482687443 1108 2 0.2235915936373445 1108 3 0.21635406665381396 1108 4 0.21816292416465188 1108 5 0.2389328594978295 1108 6 0.2484638110237185 1108 7 0.2683463440997613 1108 8 0.2588193500791789 1108 9 0.2930564778914615 1108 10-11 0.28832795932095223 1108 12-13 0.2971369651135589 1108 14-15 0.3275012920480691 1108 16-17 0.3169939891859812 1108 18-19 0.36192048641063934 1108 20-21 0.3653253008753907 1108 22-23 0.3688213362331041 1108 24-25 0.36919094293163823 1108 26-27 0.35111928923753055 1108 28-29 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-1.4659398698503843 2316 100 -1.860358884559158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 277961.0 3 47133.0 4 27230.0 5 25316.0 6 27122.0 7 26989.0 8 26079.0 9 30104.0 10 35356.0 11 40623.0 12 42255.0 13 44873.0 14 44615.0 15 44834.0 16 51327.0 17 62502.0 18 82044.0 19 126744.0 20 277117.0 21 315528.0 22 141205.0 23 118876.0 24 133262.0 25 148168.0 26 160080.0 27 184290.0 28 219210.0 29 266173.0 30 325132.0 31 410374.0 32 529980.0 33 716053.0 34 1049861.0 35 1814460.0 36 3601100.0 37 8409583.0 38 1.1326688E7 39 3284308.0 40 5985.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.149125130535563 32.52465026629617 27.46617396371638 22.86005063945189 2 22.199272281325204 29.932443224028898 28.22662976403723 19.641654730608664 3 23.59718933104919 26.577724840642762 26.8288472152956 22.996238613012448 4 21.017055806270697 27.550893281582802 26.506991734723297 24.925059177423208 5 22.472782141692957 29.543513731804627 26.420582763037164 21.563121363465253 6 25.124084742973373 26.49776820060059 26.760808400326187 21.617338656099847 7 22.055453211307512 26.843634067385675 29.432101164174508 21.668811557132305 8 24.437410323073287 26.442330781957345 27.0349966365471 22.08526225842227 9 24.457159921446358 26.020416593072078 27.417425127251732 22.104998358229828 10-11 21.687157253808653 28.184016550729634 28.31635268363446 21.812473511827253 12-13 21.77997830913396 26.738958109611332 28.06511571625922 23.41594786499549 14-15 21.792688954445925 29.776291572193486 26.525167076303998 21.905852397056595 16-17 23.428532418001105 26.616512716815215 26.545090455614474 23.40986440956921 18-19 21.97602905535897 28.117420195506458 26.47296621317444 23.433584535960126 20-21 23.540317077797408 28.0460720963691 26.447308970695126 21.966301855138365 22-23 23.46647061988302 26.60365416469786 27.999111190178503 21.930764025240617 24-25 22.07840380725519 27.91683933523531 26.355647284638934 23.649109572870568 26-27 22.131121626213947 26.397772497965654 29.423923106815543 22.047182769004852 28-29 22.132618705127037 26.46183742644801 29.409400876145586 21.996142992279363 30-31 22.16459121069842 26.4099458127532 29.351451295455313 22.074011681093076 32-33 22.194547412134767 26.41865551817896 29.2981854740841 22.088611595602178 34-35 22.19609712497791 26.501944038938614 29.25516763871403 22.04679119736945 36-37 22.205846158646423 26.452119471470432 29.20627425120476 22.135760118678387 38-39 22.22829629235525 26.401606437859154 29.23868308325563 22.13141418652997 40-41 22.230544344719007 26.472350361661114 29.23634786818724 22.060757425432637 42-43 22.2436947464239 26.435237352833468 29.181736694769185 22.139331205973452 44-45 22.29280796351535 26.399492213454018 29.185904373843275 22.121795449187356 46-47 22.253970326339527 26.481924401746525 29.179229005318657 22.084876266595288 48-49 22.314288876960873 26.42484721848562 29.094003051045945 22.16686085350756 50-51 22.297791290513068 26.42654605203169 29.09140423077366 22.184258426681584 52-53 22.367277180392907 26.55057816098905 28.939860005910894 22.14228465270714 54-55 22.5084862599477 26.663777448392338 28.50532733278358 22.32240895887638 56-57 22.70068224903591 26.885026664222885 28.0117926967885 22.402498389952704 58-59 22.731229182256193 27.15263402040855 27.75489910970983 22.36123768762543 60-61 22.761861331929822 27.21156376521165 27.62695199924546 22.39962290361307 62-63 22.723071294719706 27.26053576224435 27.613716073236986 22.402676869798963 64-65 22.707031887079335 27.312037277975964 27.661707538631948 22.319223296312757 66-67 22.71201576148368 27.16849299837842 27.748265770690768 22.371225469447133 68-69 22.652030312820965 27.029256665187322 27.955350246441686 22.363362775550026 70-71 22.61420685294179 26.995713998081015 28.11486591702988 22.27521323194731 72-73 22.689724265545436 26.96061519348446 28.053527956093117 22.296132584876986 74-75 22.662699200221546 26.965294399687128 28.095853437966973 22.27615296212435 76-77 22.7127507712932 27.08816087681744 27.94939012112845 22.24969823076091 78-79 22.805363251267096 27.123625686915435 27.7585567707167 22.312454291100764 80-81 22.847000434674356 27.211798301490187 27.614712417543945 22.326488846291507 82-83 22.874204579128925 27.35177323559948 27.48290435193913 22.29111783333247 84-85 22.898090412864583 27.380248556219804 27.37654137746391 22.345119653451707 86-87 22.963399321726115 27.37819941913816 27.3189182953544 22.33948296378133 88-89 22.95998924137986 27.461476907689804 27.26050395016516 22.31802990076518 90-91 23.044917655991956 27.434065186242456 27.161953533776618 22.359063623988966 92-93 23.078456884846858 27.432134893249405 27.151026484013347 22.33838173789039 94-95 23.114858824816707 27.523454939891973 27.10449075529089 22.257195480000426 96-97 23.160542535290833 27.463818159375226 27.04862271314038 22.32701659219356 98-99 23.18148880876253 27.43973835071011 27.075460502316222 22.303312338211136 100 23.17232183952507 27.519184195951986 27.02728397619888 22.28120998832407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 163350.0 1 101913.0 2 29741.0 3 16278.0 4 11749.0 5 8882.0 6 7625.0 7 7280.5 8 7004.5 9 7010.0 10 7537.0 11 11297.5 12 98077.5 13 114880.5 14 46083.5 15 46068.5 16 51612.0 17 57557.5 18 63054.0 19 69294.5 20 77839.5 21 92038.5 22 113893.5 23 141566.5 24 172709.0 25 206574.5 26 243237.0 27 287861.5 28 345615.0 29 406324.5 30 458268.0 31 508114.0 32 560583.0 33 607464.5 34 654344.5 35 713242.0 36 777195.5 37 830826.5 38 886617.5 39 936167.5 40 964818.5 41 1003923.0 42 1055465.5 43 1086592.0 44 1108593.5 45 1144807.0 46 1182637.5 47 1206219.5 48 1213016.0 49 1215051.0 50 1215138.0 51 1213894.0 52 1208794.5 53 1185221.0 54 1143537.0 55 1094468.0 56 1039516.5 57 965538.0 58 867506.0 59 757378.0 60 636927.5 61 517540.0 62 408892.0 63 310624.0 64 230737.0 65 169249.0 66 123579.5 67 91284.5 68 66940.5 69 50017.0 70 38093.0 71 28838.0 72 22593.0 73 17330.0 74 13352.5 75 11065.5 76 8468.0 77 5305.5 78 3180.0 79 2165.5 80 1554.5 81 1145.0 82 844.0 83 619.5 84 478.5 85 393.0 86 342.5 87 309.5 88 279.5 89 243.0 90 201.0 91 159.0 92 133.5 93 116.0 94 93.0 95 68.5 96 63.0 97 41.5 98 22.5 99 20.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14815710130913892 2 0.24092086674585383 3 0.25574672164551626 4 0.2842332322914366 5 0.2953374063130606 6 0.33089627002939664 7 0.34893656736967016 8 0.35844366493973717 9 0.35655094094179396 10-11 0.3672638167402597 12-13 0.37637526832913343 14-15 0.3688304588855711 16-17 0.3736274852509555 18-19 0.3768433769442449 20-21 0.38252009968539624 22-23 0.3861765641929083 24-25 0.38945622300404575 26-27 0.39144169917340427 28-29 0.3936677512873712 30-31 0.38536063493498945 32-33 0.4033791934850875 34-35 0.4132123729077864 36-37 0.4158891426047245 38-39 0.41241673318736466 40-41 0.41637899001001144 42-43 0.41655579883677185 44-45 0.4207455883299218 46-47 0.43001500846073715 48-49 0.4186977942953936 50-51 0.42495131960253374 52-53 0.43110194796951007 54-55 0.4309715152284572 56-57 0.4253136327721247 58-59 0.4344439246458171 60-61 0.4306599259026091 62-63 0.41823403343831717 64-65 0.4402655726548048 66-67 0.44056411870654777 68-69 0.447184304941314 70-71 0.44558288073172186 72-73 0.456771111408691 74-75 0.46493475174591475 76-77 0.455775474818655 78-79 0.4447075321139901 80-81 0.4467712679279803 82-83 0.45189872390403163 84-85 0.42976573700005855 86-87 0.45133206610679133 88-89 0.44674083362173467 90-91 0.46121596937323295 92-93 0.46133335884018045 94-95 0.46518837096462834 96-97 0.4723882582707401 98-99 0.48522863700104407 100 0.48803294093367816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.450054E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.85818445747029 #Duplication Level Percentage of deduplicated Percentage of total 1 67.83438257128928 27.037553330850557 2 14.864353494961064 11.849322868864023 3 5.8305383799898305 6.97184022707984 4 3.074415577795409 4.90162492794758 5 1.8426610225638473 3.672256146497032 6 1.2625127386074357 3.019287936919269 7 0.9003103017848889 2.5119313854251994 8 0.6644307381832231 2.118640233737604 9 0.534353266843412 1.91685159677669 >10 2.8902167250933455 20.668354444883253 >50 0.19827952507701913 5.481751219580736 >100 0.10176534258943017 6.19345744548134 >500 0.001334676235339574 0.35692984780722253 >1k 4.091056464618074E-4 0.267510152686323 >5k 7.306667992800025E-6 0.01768642951764841 >10k+ 2.922667178887911E-5 3.0150018059456065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 912649 2.645318015312224 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50047 0.14506149758815368 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45941 0.13316023459342957 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.55068471392042E-4 8.69551607018325E-6 0.0 0.0 0.0 2 3.536176535207855E-4 1.1594021426910999E-5 0.0 0.0 2.8985053567277498E-6 3 5.56513028491728E-4 1.73910321403665E-5 0.0 0.0 2.8985053567277498E-6 4 7.391188659655762E-4 3.4782064280733E-5 0.0 0.0 2.8985053567277498E-6 5 8.173785105972254E-4 4.347758035091625E-5 0.0 0.0 2.8985053567277498E-6 6 8.927396498721469E-4 5.2173096421099495E-5 0.0 0.0 2.8985053567277498E-6 7 9.449127462932464E-4 5.5071601777827244E-5 0.0 0.0 2.8985053567277498E-6 8 0.0010579544552056287 5.7970107134554994E-5 0.0 0.0 2.8985053567277498E-6 9 0.0011333155944805503 6.37671178480105E-5 0.0 0.0 2.8985053567277498E-6 10-11 0.0013014289051707596 7.681039195328537E-5 0.0 0.0 4.347758035091625E-6 12-13 0.0014956287640715189 8.69551607018325E-5 0.0 0.0 5.7970107134554995E-6 14-15 0.0017318569506448306 9.709992945037962E-5 0.0 0.0 1.0144768748547124E-5 16-17 0.002002867201498875 1.1449096159074611E-4 0.0 0.0 1.8840284818730374E-5 18-19 0.0023361953175225665 1.2608498301765712E-4 0.0 0.0 2.3188042853821998E-5 20-21 0.002578220514809333 1.5362078390657074E-4 0.0 0.0 2.6086548210549747E-5 22-23 0.00275647859424809 1.62316299976754E-4 0.0 0.0 2.8985053567277497E-5 24-25 0.0030535753933126843 1.73910321403665E-4 0.0 1.4492526783638749E-6 3.0434306245641375E-5 26-27 0.0034492213745060223 1.8840284818730373E-4 2.8985053567277498E-6 5.7970107134554995E-6 3.188355892400525E-5 28-29 0.003983995612822292 2.0579388032767023E-4 2.8985053567277498E-6 8.69551607018325E-6 3.4782064280733E-5 30-31 0.00497818295017991 2.2318491246803673E-4 2.8985053567277498E-6 8.69551607018325E-6 4.05790749941885E-5 32-33 0.0068506174106260365 2.4492370264349485E-4 2.8985053567277498E-6 8.69551607018325E-6 4.347758035091625E-5 34-35 0.010205637361038408 2.594162294271336E-4 2.8985053567277498E-6 8.69551607018325E-6 4.347758035091625E-5 36-37 0.015279470987990332 2.6956099817568074E-4 2.8985053567277498E-6 8.69551607018325E-6 4.782533838600787E-5 38-39 0.022153276441470193 2.8115501960259174E-4 2.8985053567277498E-6 8.69551607018325E-6 5.5071601777827244E-5 40-41 0.03181544404812214 2.840535249593195E-4 2.8985053567277498E-6 1.0144768748547124E-5 5.5071601777827244E-5 42-43 0.0444862602150575 2.884012829944111E-4 2.8985053567277498E-6 1.1594021426910999E-5 5.941935981291887E-5 44-45 0.06017731896370317 3.01444557099686E-4 2.8985053567277498E-6 1.1594021426910999E-5 6.086861249128274E-5 46-47 0.07941180051094852 3.1593708388332473E-4 2.8985053567277498E-6 1.3043274105274874E-5 6.666562320473825E-5 48-49 0.1024766568871096 3.202848419184164E-4 2.8985053567277498E-6 1.73910321403665E-5 7.246263391819375E-5 50-51 0.12952695812877132 3.289803579885996E-4 2.8985053567277498E-6 1.73910321403665E-5 7.970889731001312E-5 52-53 0.16125544701619163 3.376758740587829E-4 2.8985053567277498E-6 1.73910321403665E-5 8.260740266674087E-5 54-55 0.19720271045032917 3.565161588775132E-4 2.8985053567277498E-6 1.73910321403665E-5 8.69551607018325E-5 56-57 0.23802381064180445 3.8115345440969907E-4 2.8985053567277498E-6 1.8840284818730374E-5 8.69551607018325E-5 58-59 0.28408251001288676 4.1738477136879596E-4 2.8985053567277498E-6 2.028953749709425E-5 9.420142409365187E-5 60-61 0.3349251924752482 4.318772981524347E-4 2.8985053567277498E-6 2.028953749709425E-5 9.565067677201574E-5 62-63 0.39096924280025763 4.3912356154425405E-4 2.8985053567277498E-6 2.3188042853821998E-5 9.565067677201574E-5 64-65 0.45213929984864004 4.492683302928012E-4 2.8985053567277498E-6 2.6086548210549747E-5 9.565067677201574E-5 66-67 0.5186715918069689 4.6376085707643995E-4 2.8985053567277498E-6 2.6086548210549747E-5 9.565067677201574E-5 68-69 0.5905965529814896 4.8260114189517035E-4 2.8985053567277498E-6 2.6086548210549747E-5 9.854918212874349E-5 70-71 0.6682243234453722 4.97093668678809E-4 2.8985053567277498E-6 2.6086548210549747E-5 1.0144768748547124E-4 72-73 0.7528577813564656 5.188324588542672E-4 2.8985053567277498E-6 3.0434306245641375E-5 1.0144768748547124E-4 74-75 0.8434679573131318 5.391219963513615E-4 2.8985053567277498E-6 3.768056963746075E-5 1.0144768748547124E-4 76-77 0.9404693375813828 5.507160177782725E-4 2.8985053567277498E-6 3.912982231582462E-5 1.0144768748547124E-4 78-79 1.04434742180847 5.768025659888222E-4 2.8985053567277498E-6 4.347758035091625E-5 1.0434619284219899E-4 80-81 1.155065978677435 6.14483135626283E-4 2.8985053567277498E-6 4.347758035091625E-5 1.0724469819892674E-4 82-83 1.2739858564532613 6.434681891935605E-4 2.8985053567277498E-6 4.6376085707643996E-5 1.0724469819892674E-4 84-85 1.4011780685171886 6.681054847257463E-4 2.8985053567277498E-6 4.6376085707643996E-5 1.0724469819892674E-4 86-87 1.5364469657576374 6.898442749012045E-4 2.8985053567277498E-6 4.6376085707643996E-5 1.1304170891238224E-4 88 1.6438931100788567 7.07235307041571E-4 2.8985053567277498E-6 4.6376085707643996E-5 1.1594021426910999E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 122365 0.0 79.78286 8 AGTGGTA 124845 0.0 78.42511 5 GTATCAA 124540 0.0 78.171326 9 GTGGTAT 125530 0.0 77.88673 6 TGGTATC 126405 0.0 77.15784 7 AAGCAGT 140230 0.0 70.77197 1 GCAGTGG 140605 0.0 70.30995 3 CAGTGGT 141575 0.0 69.38002 4 AGCAGTG 151475 0.0 65.576706 2 CAACGCA 116185 0.0 44.13984 12-13 ATCAACG 119440 0.0 43.018898 10-11 ACGCAGA 121700 0.0 42.221867 14-15 TCAACGC 116610 0.0 41.73923 12-13 AGAGTAC 123960 0.0 41.03766 18-19 AACGCAG 119550 0.0 40.615826 14-15 GAGTACT 119175 0.0 39.474773 20-21 CGCAGAG 123685 0.0 39.248917 16-17 TATCAAC 125530 0.0 38.80828 10-11 AGTACTT 130190 0.0 38.397533 20-21 GTACTTT 129560 0.0 36.407684 22-23 >>END_MODULE